作者: Obedient 時(shí)間: 2025-3-21 20:43
Culture of the Mouse Preimplantation Embryo,sed on balanced salt solutions, containing the carbohydrates pyruvate, lactate, and glucose as the sole energy sources. Such media used a bicarbonate–carbon dioxide buffer system to maintain the desired pH, and were typically supplemented with serum albumin, but lacked free amino acids. In contrast 作者: Hallowed 時(shí)間: 2025-3-22 01:51
In Vitro Culture of Rat Preimplantation Embryos,ed animal models to study human diseases and conditions. In addition to in vitro fertilization (IVF), the ability to grow IVF-derived or in vivo-collected zygotes to a desired preimplantation stage (zygote to blastocyst) entirely in vitro has a great importance for studies of developmental biology a作者: 媽媽不開心 時(shí)間: 2025-3-22 07:46
Enabling Hamster Embryo Culture System: Development of Preimplantation Embryos,es being studied. While the mouse model has been versatile for studying in vitro development of early embryos, other rodent species are important to gain insights into comparative early embryogenesis. The golden hamster (.) offers unique advantages to study cellular and molecular regulation of gamet作者: 豪華 時(shí)間: 2025-3-22 08:57 作者: Iniquitous 時(shí)間: 2025-3-22 14:38
In Vitro Maturation, Fertilization, and Culture of Pig Oocytes and Embryos,roduction agriculture. Methods of oocyte maturation, fertilization, and culture all play an extremely important role in how the embryo, fetus, and offspring will develop. In this chapter, we discuss the historical methods and recent advances that have been essential in promoting efficient and compet作者: Iniquitous 時(shí)間: 2025-3-22 20:36 作者: creditor 時(shí)間: 2025-3-23 00:49
Production and Culture of the Bovine Embryo,for in vitro maturation of oocytes, capacitation of sperm, fertilization, and development of the resultant embryos to the blastocyst stage. The protocol can be easily modified to use oocytes collected by ultrasound-guided follicular aspiration.作者: 虛度 時(shí)間: 2025-3-23 03:53
In Vitro Embryo Production in Sheep,o revolutionize the productive efficiency of livestock species. The development of technology for sequencing of whole genomes and mass screening of gene regulation has widened our approach to genetic profiling and mapping, as well as furthering our understanding of underlying physiological mechanism作者: mortgage 時(shí)間: 2025-3-23 07:45 作者: 審問 時(shí)間: 2025-3-23 12:34 作者: 藥物 時(shí)間: 2025-3-23 15:32
In Vitro Production of Bison Embryos,ver the last few decades (e.g., superovulation and embryo transfer), but their efficiencies are low. Since 2007, several methods for producing bison embryos in vitro have been published. All of these methods are based on cattle embryo production models and have varying degrees of success with regard作者: photopsia 時(shí)間: 2025-3-23 20:12
In Vitro Embryo Production in Buffalo Species (,),nditions make buffalo an irreplaceable milk producer. Since cattle cannot express their own productive potential in these conditions, buffaloes are best suited to satisfy animal protein demand, as they are easy to maintain, long-lived, and parasite resistant. Here we describe how to produce buffalo 作者: 生命層 時(shí)間: 2025-3-23 23:46 作者: 貪婪地吃 時(shí)間: 2025-3-24 02:40
In Vitro Culture of Camelid Embryos,on occurs in response to copulation. Little is known about the early embryonic development and placentation in camelid species. Here we describe protocols for the culture of both in vitro-produced and in vivo-retrieved camel embryos. A chemically defined medium enables the development of in vitro-pr作者: BILK 時(shí)間: 2025-3-24 09:29
In Vitro Culture of Embryos from Horses, failure of standard in vitro fertilization techniques in horses. However, the development of intracytoplasmic sperm injection (ICSI) for the assisted fertilization of equine oocytes has resulted in a renewed interest in establishing optimal methods for embryo culture. Currently, ICSI-produced equin作者: arousal 時(shí)間: 2025-3-24 10:56 作者: Alveolar-Bone 時(shí)間: 2025-3-24 17:45
The Domestic Dog Embryo: In Vitro Fertilization, Culture, and Transfer,aking advantage of the dog as a biomedical research model for human and companion animal medicine, developing assisted reproductive technologies to manage genetic diversity in endangered canids maintained ex situ, and improving breeding in rare or working breeds of dogs. Here, we focus on recent adv作者: maladorit 時(shí)間: 2025-3-24 19:38 作者: MELON 時(shí)間: 2025-3-25 02:51
Historical Note: Lessons Learned from Developing the First Semi-Defined Medium for Culture of One-Cdium that stimulated this “renewed interest” referred to above and includes some of the initial difficulties in getting the work published. The phrase “semi-defined medium” is understood here to mean a defined medium (all of whose components are chemically known) supplemented with an undefined compo作者: 訓(xùn)誡 時(shí)間: 2025-3-25 04:43
Culture of the Mouse Preimplantation Embryo,ly more complex. A main addition to such media has been an array of amino acids. Although the media of today contain more components than their predecessors, their preparation remains relatively easy to accomplish, made feasible through the use of stock solutions, which also readily facilitates any 作者: 加劇 時(shí)間: 2025-3-25 07:51 作者: lactic 時(shí)間: 2025-3-25 15:17
Culture of Preimplantation Rabbit Embryos,tro blastocyst growth comparable to in vivo growth still remain an unsolved problem. Citrate, often found as a contaminant in serum albumin, may have an essential role in rabbit blastocyst growth, which would fit in with its role in the development of serum-free media for culture of various types of作者: patella 時(shí)間: 2025-3-25 16:10
In Vitro Culture of Embryos from LOPU-Derived Goat Oocytes,efore, strategies to improve in vitro embryo quality are crucial, such as the use of oviductal epithelium cells for coculture. Here we describe the main steps and culture media which can be utilized to produce embryos in vitro from LOPU or slaughterhouse oocytes in goats.作者: Angiogenesis 時(shí)間: 2025-3-25 22:41 作者: 閑逛 時(shí)間: 2025-3-26 02:26 作者: Grating 時(shí)間: 2025-3-26 04:22 作者: MOTIF 時(shí)間: 2025-3-26 10:17 作者: Airtight 時(shí)間: 2025-3-26 16:24 作者: Infraction 時(shí)間: 2025-3-26 18:45
Was bleibt? - ein synoptischer Versuch!comprehensive perspective (historical and current) on the embryo culture systems and details an optimized culture protocols to achieve normal and viable development of preimplantation embryos in the golden hamster.作者: fabricate 時(shí)間: 2025-3-27 01:01
Was bleibt? - ein synoptischer Versuch!tro blastocyst growth comparable to in vivo growth still remain an unsolved problem. Citrate, often found as a contaminant in serum albumin, may have an essential role in rabbit blastocyst growth, which would fit in with its role in the development of serum-free media for culture of various types of作者: 銼屑 時(shí)間: 2025-3-27 04:40
Was bleibt? - ein synoptischer Versuch!efore, strategies to improve in vitro embryo quality are crucial, such as the use of oviductal epithelium cells for coculture. Here we describe the main steps and culture media which can be utilized to produce embryos in vitro from LOPU or slaughterhouse oocytes in goats.作者: 預(yù)防注射 時(shí)間: 2025-3-27 06:44
https://doi.org/10.1007/978-3-658-39798-2 has been formulated based on deer oviduct fluid. Red deer calves (.) and Thamin Eld’s deer fawn (.) have been produced after the transfer of in vitro embryos (IVF and SCNT) grown in DSOF culture. Here we describe the in vitro method of maturation, fertilization, and embryo culture for deer species.作者: Germinate 時(shí)間: 2025-3-27 10:25
https://doi.org/10.1007/978-3-8350-5490-5of the development of rat in vitro culture systems and a step-by-step protocol to produce rat blastocyst stage embryos from zygotes under in vitro conditions from commonly used laboratory rat strains in biomedical research.作者: Palliation 時(shí)間: 2025-3-27 13:53
https://doi.org/10.1007/978-3-8350-5490-5embryos have resulted in commercial in vitro production of embryos. Here we describe in vitro maturation, in vitro fertilization, embryo production, embryo culture, and quantitation of gene expression in sheep embryos.作者: engender 時(shí)間: 2025-3-27 19:01
https://doi.org/10.1007/978-3-322-90706-6s. Methods to age match prediapause embryos in a mammal that exhibits superfetation, and to synchronize collection of reactivation from diapause stages using prolactin will be discussed. Finally, a quantitative method to determine the extent of cell proliferation in the blastocyst, a hallmark of reactivation from diapause, will be detailed.作者: Omniscient 時(shí)間: 2025-3-28 01:39
In Vitro Culture of Rat Preimplantation Embryos,of the development of rat in vitro culture systems and a step-by-step protocol to produce rat blastocyst stage embryos from zygotes under in vitro conditions from commonly used laboratory rat strains in biomedical research.作者: PHONE 時(shí)間: 2025-3-28 05:37 作者: 不知疲倦 時(shí)間: 2025-3-28 10:13 作者: Maximizer 時(shí)間: 2025-3-28 11:34 作者: 縱火 時(shí)間: 2025-3-28 15:09 作者: 彈藥 時(shí)間: 2025-3-28 20:41
Was bleibt? - ein synoptischer Versuch!st suited to satisfy animal protein demand, as they are easy to maintain, long-lived, and parasite resistant. Here we describe how to produce buffalo embryos from slaughterhouse oocytes, with protocols for media formulation, oocyte selection, semen capacitation, and in the end embryo evaluation.作者: pacific 時(shí)間: 2025-3-29 02:37 作者: 裙帶關(guān)系 時(shí)間: 2025-3-29 06:56 作者: 不可思議 時(shí)間: 2025-3-29 07:55
In Vitro Maturation, Fertilization, and Culture of Pig Oocytes and Embryos,spring will develop. In this chapter, we discuss the historical methods and recent advances that have been essential in promoting efficient and competent embryo development. Here we describe the procedures that can be used to mature, fertilize, and culture pig embryos to the blastocyst stage.作者: ingenue 時(shí)間: 2025-3-29 12:16 作者: justify 時(shí)間: 2025-3-29 18:53
In Vitro Culture of Camelid Embryos,cols for the culture of both in vitro-produced and in vivo-retrieved camel embryos. A chemically defined medium enables the development of in vitro-produced embryos from cleavage to the hatching blastocyst stage. In vivo-retrieved embryos will survive in vitro for 23?days postinsemination, reaching a diameter of ~5?mm.作者: gospel 時(shí)間: 2025-3-29 21:54 作者: Incumbent 時(shí)間: 2025-3-30 03:36 作者: Deduct 時(shí)間: 2025-3-30 05:17 作者: 昆蟲 時(shí)間: 2025-3-30 11:09 作者: biosphere 時(shí)間: 2025-3-30 15:52
https://doi.org/10.1007/978-3-531-91757-3l embryo culture medium was subsequently modified for both IVM and IVF. Using a small number of stock solutions, a series of media can be easily prepared that allows for immature cat oocytes to be matured and fertilized in vitro, with the resulting embryos developing to the blastocyst stage without the use of serum.作者: 牢騷 時(shí)間: 2025-3-30 18:19 作者: 襲擊 時(shí)間: 2025-3-30 22:35 作者: 慟哭 時(shí)間: 2025-3-31 02:58 作者: Osteoarthritis 時(shí)間: 2025-3-31 06:39
In Vitro Culture of Late Stage Pig Embryos in a Chemically Defined Medium, Porcine Blastocyst Mediune blastocyst medium (PBM) particularly designed for the late stage embryo culture could improve the potential of morulae or blastocysts to develop into hatching and hatched blastocysts with good quality.作者: 蚊子 時(shí)間: 2025-3-31 09:13
In Vitro Production of Bison Embryos, to embryo production rates. In this chapter, a brief summary of these reports is presented followed by a detailed protocol that has been successfully used to produce bison embryos in vitro and live offspring following embryo transfer.作者: botany 時(shí)間: 2025-3-31 15:36
In Vitro Culture of Embryos from Horses,e embryos are cultured using media designed for other species or other cell cultures and, typically, with the addition of serum. Although systems specifically for horse embryo culture still have not been established, ICSI-produced embryos are developmentally competent and capable of producing live offspring.作者: fabricate 時(shí)間: 2025-3-31 18:28
In Vitro Culture of Embryos from Domestic Cats,l embryo culture medium was subsequently modified for both IVM and IVF. Using a small number of stock solutions, a series of media can be easily prepared that allows for immature cat oocytes to be matured and fertilized in vitro, with the resulting embryos developing to the blastocyst stage without the use of serum.作者: 我的巨大 時(shí)間: 2025-3-31 21:50
https://doi.org/10.1007/978-3-8350-5490-5nd differentiation in vitro, Plenum Press, New York, 1987) summarized the history of early preimplantation embryo culture as follows “The culture of early mammalian embryos has been studied predominantly in two species: the rabbit and the mouse. Up to 1949, the rabbit was used almost exclusively, wi作者: avulsion 時(shí)間: 2025-4-1 04:59
Was bleibt? - ein synoptischer Versuch!sed on balanced salt solutions, containing the carbohydrates pyruvate, lactate, and glucose as the sole energy sources. Such media used a bicarbonate–carbon dioxide buffer system to maintain the desired pH, and were typically supplemented with serum albumin, but lacked free amino acids. In contrast 作者: Limpid 時(shí)間: 2025-4-1 08:01
https://doi.org/10.1007/978-3-8350-5490-5ed animal models to study human diseases and conditions. In addition to in vitro fertilization (IVF), the ability to grow IVF-derived or in vivo-collected zygotes to a desired preimplantation stage (zygote to blastocyst) entirely in vitro has a great importance for studies of developmental biology a作者: 倒轉(zhuǎn) 時(shí)間: 2025-4-1 10:50 作者: Obliterate 時(shí)間: 2025-4-1 17:14
