標題: Titlebook: Collagen; Methods and Protocol Irit Sagi,Nikolaos A. Afratis Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 E [打印本頁] 作者: tricuspid-valve 時間: 2025-3-21 19:40
書目名稱Collagen影響因子(影響力)
書目名稱Collagen影響因子(影響力)學科排名
書目名稱Collagen網(wǎng)絡公開度
書目名稱Collagen網(wǎng)絡公開度學科排名
書目名稱Collagen被引頻次
書目名稱Collagen被引頻次學科排名
書目名稱Collagen年度引用
書目名稱Collagen年度引用學科排名
書目名稱Collagen讀者反饋
書目名稱Collagen讀者反饋學科排名
作者: 小溪 時間: 2025-3-21 21:06 作者: Harrowing 時間: 2025-3-22 01:01
Production and Characterization of Chemically Cross-Linked Collagen Scaffoldsntation. Herein, we describe techniques to produce cross-linked with glutaraldehyde (GTA; amine terminal cross-linker), 4-arm polyethylene glycol succinimidyl glutarate (4SP; amine terminal cross-linker), diphenyl phosphoryl azide (DPPA; carboxyl terminal cross-linker), and 1-ethyl-3-(3-dimethylamin作者: NOT 時間: 2025-3-22 07:21 作者: 軟膏 時間: 2025-3-22 11:49
Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopydentifying features on an atomic length scale in tissues and protein samples without extensive extraction or purification. Hydroxylation is a key posttranslational modification of collagen that gives rise to distinctive signals in the ssNMR spectrum of collagen proteins. Here we outline the type of 作者: 致詞 時間: 2025-3-22 16:30
Measurement of Collagen Cross-Links from Tissue Samples by Mass Spectrometrych as fibrosis or cancer. One of the key contributors in the establishment of a fibrotic state is the abnormal deposition of extracellular matrix and cross-linked proteins, in particular collagen, leading to tissue stiffening and disruption of organ function. The precise and sensitive measurement of作者: 致詞 時間: 2025-3-22 20:32
Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomess that enable robust and accurate determination of collagen interactomes via mass spectrometry-based proteomics. Using collagen type-I as an example, protocols for working with large, repetitive, and GC-rich collagen genes are described, followed by strategies for engineering cells that stably and i作者: defeatist 時間: 2025-3-22 23:49 作者: 粗糙濫制 時間: 2025-3-23 01:34 作者: 旁觀者 時間: 2025-3-23 09:01
In Situ Detection of Degraded and Denatured Collagen via Triple Helical Hybridization: New Tool in H arthritis, and fibrosis). Here we describe histological staining of such collagens using fluorescent collagen hybridizing peptide that can specifically bind to collagen strands by folding into triple helix. The method can report the amount of denatured collagen and/or collagen remodeling activity i作者: parsimony 時間: 2025-3-23 13:23 作者: DOSE 時間: 2025-3-23 14:51
Analysis of Collagen-Binding Integrin Interactions with Supramolecular Aggregates of the Extracellul macromolecules forming aggregates or suprastructures. Moreover, the molecular composition is important for the macromolecular organization and, thereby, the functional properties of the ECM. In addition, collagen molecules lose their integrin-binding capabilities after incorporation into fibrils. T作者: 漸強 時間: 2025-3-23 19:24
Assessing Collagen Deposition During Aging in Mammalian Tissue and in urring: a local excess in collagen deposition, for instance during fibrosis, or a gradual overall reduction of collagen mass. Here, we describe a histological and a colorimetric method to assess collagen levels in mammalian tissues and in the nematode .. The first method is the polychrome Herovici s作者: Decimate 時間: 2025-3-24 00:39 作者: 相信 時間: 2025-3-24 05:31 作者: obscurity 時間: 2025-3-24 08:05 作者: 替代品 時間: 2025-3-24 13:03 作者: 不確定 時間: 2025-3-24 15:05
https://doi.org/10.1007/978-3-030-23573-4 and therapeutic interests in the ability to modify collagens, as this would allow for studies precisely describing interactions of collagens with specific interaction partners, addressing consequences of individual disease-causing mutations, and assessing therapeutic applicability of precision medi作者: hysterectomy 時間: 2025-3-24 20:33
Cristina Cavaco,Jo?o Pedro Costacollagen, which can be employed to?a wide range of tissue sources, and subsequently use it to study the?collagen crosslinking and stabilization abilities of various compounds. The protocol is designed for a multi-well format assay and thus can be used for simultaneous assessment of multiple number o作者: 殘酷的地方 時間: 2025-3-24 23:16 作者: Acetaminophen 時間: 2025-3-25 05:48 作者: Enzyme 時間: 2025-3-25 09:17
Juan Martín Dabezies,Paula Ballesteros-Ariasdentifying features on an atomic length scale in tissues and protein samples without extensive extraction or purification. Hydroxylation is a key posttranslational modification of collagen that gives rise to distinctive signals in the ssNMR spectrum of collagen proteins. Here we outline the type of 作者: Watemelon 時間: 2025-3-25 12:01
https://doi.org/10.1007/978-94-007-6796-6ch as fibrosis or cancer. One of the key contributors in the establishment of a fibrotic state is the abnormal deposition of extracellular matrix and cross-linked proteins, in particular collagen, leading to tissue stiffening and disruption of organ function. The precise and sensitive measurement of作者: Bravado 時間: 2025-3-25 17:53
Juan Martín Dabezies,Paula Ballesteros-Ariass that enable robust and accurate determination of collagen interactomes via mass spectrometry-based proteomics. Using collagen type-I as an example, protocols for working with large, repetitive, and GC-rich collagen genes are described, followed by strategies for engineering cells that stably and i作者: conspicuous 時間: 2025-3-25 23:54 作者: ferment 時間: 2025-3-26 00:31
Valbona Zeneli,Marios P. Efthymiopoulosurfaces, imaging of the extracellular matrix requires cell removal before sample preparation. In this protocol, we will describe a method for preparing extracellular matrices derived from murine colon for imaging under a scanning electron microscope.作者: 笨拙處理 時間: 2025-3-26 06:52 作者: Fecundity 時間: 2025-3-26 11:12 作者: 善于騙人 時間: 2025-3-26 15:52 作者: 粗語 時間: 2025-3-26 18:55 作者: Cursory 時間: 2025-3-27 00:02 作者: Crohns-disease 時間: 2025-3-27 04:22 作者: receptors 時間: 2025-3-27 08:17 作者: 礦石 時間: 2025-3-27 11:01
Irit Sagi,Nikolaos A. AfratisIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: Gullible 時間: 2025-3-27 16:41 作者: 杠桿 時間: 2025-3-27 20:54 作者: Largess 時間: 2025-3-27 22:23 作者: 勉勵 時間: 2025-3-28 03:30 作者: 樂器演奏者 時間: 2025-3-28 06:41 作者: creatine-kinase 時間: 2025-3-28 14:21
1064-3745 avoiding known pitfalls.?.Authoritative and practical, .Collagen: Methods and Protocols. is an ideal guide to high quality and repeatable protocols in this vi978-1-4939-9095-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 可觸知 時間: 2025-3-28 17:41
Juan Martín Dabezies,Paula Ballesteros-Ariasprotocols presented herein may be readily adapted to define interactomes of other collagen types, as well as to determine comparative interactomes of normal and disease-causing collagen variants using quantitative isotopic labeling (SILAC)- or isobaric mass tags (iTRAQ or TMT)-based mass spectrometr作者: 瑣碎 時間: 2025-3-28 20:03
Felicia Pratto,Iris ?e?elj,Marija Brankovi? modified (e.g., acetylated) N termini. In this chapter, we provide a protocol for tandem mass tag (TMT)-TAILS analysis and further discuss specific considerations regarding N-terminome data interpretation using Proteome Discoverer? software.作者: 水土 時間: 2025-3-29 00:32 作者: 消耗 時間: 2025-3-29 05:37 作者: 元音 時間: 2025-3-29 08:13
Cloning and Mutagenesis Strategies for Large Collagenscine approaches. Using collagen VII as an example, we will here describe a strategy for rapid and simple modification of cDNAs encoding large collagens. The method is flexible and can be used for the creation of point mutations, small or large deletions, and insertion of DNA.作者: BURSA 時間: 2025-3-29 11:36 作者: 樹上結蜜糖 時間: 2025-3-29 15:48
A Biomimetic Model for Mineralization of Type-I Collagen Fibrilsor horse tendon is self-assembled into fibrils and mineralized by HAP in vitro. The mineralization process is monitored by cryoTEM in combination with two-dimensional (2D) and three-dimensional (3D) stochastic optical reconstruction microscopy (STORM), which enables in situ and high-resolution visualization of the process.作者: bonnet 時間: 2025-3-29 20:41
Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopyinformation that ssNMR can provide and describe the procedures involved in a ssNMR structural study, with particular focus on using dynamic nuclear polarization to enhance sensitivity for detecting hydroxylysine residues by ssNMR.作者: GLIB 時間: 2025-3-30 02:19 作者: LATE 時間: 2025-3-30 04:35
Book 2019t importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beginning with a section on in vitro models for the characterization of collagen formation, the book continues by highlighting large-scale analysis of co作者: 上下倒置 時間: 2025-3-30 10:31
1064-3745 expertsThis detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and g作者: MAUVE 時間: 2025-3-30 15:42
Cristina Cavaco,Jo?o Pedro Costaies of various compounds. The protocol is designed for a multi-well format assay and thus can be used for simultaneous assessment of multiple number of compounds and can be easily adapted to a high-throughput screening setup.作者: Accolade 時間: 2025-3-30 18:34 作者: Mingle 時間: 2025-3-30 22:45
Exploring the Polyhedron Kingdomby, the functional properties of the ECM. In addition, collagen molecules lose their integrin-binding capabilities after incorporation into fibrils. Therefore, we have established detailed protocols for the analysis of integrin-matrix interactions at the supramolecular level.作者: GROG 時間: 2025-3-31 03:19 作者: 比目魚 時間: 2025-3-31 07:59
Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics skin. This multiscale approach provides simultaneously the stress/stretch response of a skin biopsy and the collagen reorganization in the dermis by use of second harmonic generation (SHG) signals and appropriate image processing.作者: Glucocorticoids 時間: 2025-3-31 10:39
Analysis of Collagen-Binding Integrin Interactions with Supramolecular Aggregates of the Extracellulby, the functional properties of the ECM. In addition, collagen molecules lose their integrin-binding capabilities after incorporation into fibrils. Therefore, we have established detailed protocols for the analysis of integrin-matrix interactions at the supramolecular level.作者: eardrum 時間: 2025-3-31 16:45
