標(biāo)題: Titlebook: Cohesin and Condensin; Methods and Protocol Kyoko Yokomori,Katsuhiko Shirahige Book 2017 Springer Science+Business Media New York 2017 cohe [打印本頁] 作者: Reticent 時(shí)間: 2025-3-21 18:30
書目名稱Cohesin and Condensin影響因子(影響力)
書目名稱Cohesin and Condensin影響因子(影響力)學(xué)科排名
書目名稱Cohesin and Condensin網(wǎng)絡(luò)公開度
書目名稱Cohesin and Condensin網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cohesin and Condensin被引頻次
書目名稱Cohesin and Condensin被引頻次學(xué)科排名
書目名稱Cohesin and Condensin年度引用
書目名稱Cohesin and Condensin年度引用學(xué)科排名
書目名稱Cohesin and Condensin讀者反饋
書目名稱Cohesin and Condensin讀者反饋學(xué)科排名
作者: 使成整體 時(shí)間: 2025-3-21 23:02
https://doi.org/10.1007/978-94-007-1324-6m chromatin is incubated with these extracts, it is first duplicated within an assembled nucleus, and is then transformed into mitotic chromosomes, in each of which sister chromatids are juxtaposed with each other in a cohesin-dependent manner. Here we describe our protocols for assembling duplicate作者: Bombast 時(shí)間: 2025-3-22 00:38 作者: 幻想 時(shí)間: 2025-3-22 04:54
Lecture Notes in Electrical Engineeringesion is terminated during the metaphase-to-anaphase transition to trigger sister-chromatid segregation. The establishment and dissolution of cohesion are highly regulated by the cohesin complex and its multitude of regulators. In particular, the cohesin regulator Wapl promotes the release of cohesi作者: 改變立場 時(shí)間: 2025-3-22 09:40
Lecture Notes in Electrical Engineeringposttranslational modifications, including phosphorylation, acetylation, ubiquitylation, and SUMOylation. Recent studies have shown that cohesin SUMOylation is essential for sister chromatid cohesion during normal cell cycle and in response to DNA damage. Posttranslational modification by the small 作者: 集中營 時(shí)間: 2025-3-22 14:55 作者: 集中營 時(shí)間: 2025-3-22 19:28
A. Bulletti,L. Capineri,D. Floridiaque chromatin structures and chromatin associated protein complexes. The analysis of these structures and complexes has greatly benefited from and relied on the visualization of meiotic proteins in diverse preparations of meiocytes. In this chapter we summarize methods that can be used for the chara作者: CROAK 時(shí)間: 2025-3-22 23:22 作者: 設(shè)施 時(shí)間: 2025-3-23 03:10 作者: Flustered 時(shí)間: 2025-3-23 05:49
Sensors and Protocols for Industry 4.0P) to the internal red fluorescence protein (RFP) control. With this dual-color heterologous reporter assay, we have revealed cohesin-regulated genes and discovered a .-acting DNA element, the Ty1-LTR, which interacts with cohesin and regulates gene expression during yeast meiosis. The method descri作者: 微粒 時(shí)間: 2025-3-23 11:48
Sensors and Sensing: A Biologist’s View. Proteins that mediate silencing affect all of these nuclear processes. Similarly proteins involved in replication, repair, and chromosome structure play a role in the formation and maintenance of silenced heterochromatin. In this chapter we describe a handful of simple tools and methods that can b作者: 即席 時(shí)間: 2025-3-23 17:55
https://doi.org/10.1007/978-3-7091-6025-1s called cohesinopathies have been associated with genetic mutations in cohesin subunit genes or genes encoding regulators of cohesin function. Studies in different model systems, from yeast to mouse have provided insights into the molecular mechanisms of action of cohesin/cohesin regulators and the作者: 有節(jié)制 時(shí)間: 2025-3-23 20:58
Sensors and Sensing: An Engineer’s Viewand the organization of chromatin in interphase cells. To study cohesin’s role in gene expression and chromatin organization, it is necessary to avoid secondary effects due to disruption of vital cohesin functions in the cell cycle. Here we describe experimental approaches to achieve this and the me作者: 凝乳 時(shí)間: 2025-3-24 01:36 作者: grovel 時(shí)間: 2025-3-24 05:16
Ruzena Bajcsy,Eric Krotkov,Max Mintzn promotes homologous recombination (HR) repair of DNA double-strand breaks (DSBs), which is conserved from yeast to humans, and is a critical effector of ATM/ATR DDR kinase-mediated checkpoint control in mammalian cells. Optical laser microirradiation has been instrumental in revealing the damage s作者: A簡潔的 時(shí)間: 2025-3-24 07:46
Ruzena Bajcsy,Eric Krotkov,Max Mintzyotic genomes, little is known about the molecular basis of this chromosome condensation process. Here, we describe in detail the protocol for a quantitative chromosome condensation assay in fission yeast cells, which is based on precise time-resolved measurements of the distances between two fluore作者: Enthralling 時(shí)間: 2025-3-24 11:04 作者: 固執(zhí)點(diǎn)好 時(shí)間: 2025-3-24 16:18 作者: 認(rèn)識 時(shí)間: 2025-3-24 21:58
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/229233.jpg作者: HILAR 時(shí)間: 2025-3-25 00:56 作者: conduct 時(shí)間: 2025-3-25 06:31 作者: 雄辯 時(shí)間: 2025-3-25 09:45
Book 2017ten in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutti作者: visceral-fat 時(shí)間: 2025-3-25 13:14 作者: 空氣傳播 時(shí)間: 2025-3-25 19:30
Zebrafish as a Model to Study Cohesin and Cohesinopathiesirst shown in zebrafish, and Nipbl, where the fish work revealed tissue-specific functions in heart, gut, and limbs, and long-range enhancer–promoter interactions that control . gene expression in vivo. Here we discuss the utility of the zebrafish in studying the developmental and pathogenic roles o作者: 逢迎白雪 時(shí)間: 2025-3-25 22:25
1064-3745 tive topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutti978-1-4939-8232-5978-1-4939-6545-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: BLOT 時(shí)間: 2025-3-26 02:19
Lecture Notes in Electrical Engineeringon. Here I describe two approaches for analysis of meiotic cohesion in . The first approach relies on cytology to detect and quantify defects in SCC. The second approach relies on PCR and restriction digests to identify embryos that inherited an incorrect complement of chromosomes due to aberrant me作者: Antecedent 時(shí)間: 2025-3-26 05:07
https://doi.org/10.1007/978-3-7091-6025-1irst shown in zebrafish, and Nipbl, where the fish work revealed tissue-specific functions in heart, gut, and limbs, and long-range enhancer–promoter interactions that control . gene expression in vivo. Here we discuss the utility of the zebrafish in studying the developmental and pathogenic roles o作者: embolus 時(shí)間: 2025-3-26 09:37 作者: CRP743 時(shí)間: 2025-3-26 14:57 作者: 抓住他投降 時(shí)間: 2025-3-26 17:15 作者: graphy 時(shí)間: 2025-3-26 21:12
Sensors and Protocols for Industry 4.0and discovered a .-acting DNA element, the Ty1-LTR, which interacts with cohesin and regulates gene expression during yeast meiosis. The method described here provides an effective cytological approach for quantitative analysis of global gene expression in budding yeast meiosis.作者: URN 時(shí)間: 2025-3-27 02:07
Sensors and Sensing: A Biologist’s Viewplay a role in the formation and maintenance of silenced heterochromatin. In this chapter we describe a handful of simple tools and methods that can be used to study the atypical role of proteins in gene silencing.作者: inchoate 時(shí)間: 2025-3-27 09:03 作者: Eructation 時(shí)間: 2025-3-27 11:37
An In Vitro Assay for Monitoring Topological DNA Entrapment by the Chromosomal Cohesin Complexcohesin function and requires its ATPase and another heterodimer complex called the cohesin loader. Here we describe the biochemical reconstitution of topological cohesin loading onto DNA using the purified fission yeast cohesin proteins.作者: NUDGE 時(shí)間: 2025-3-27 17:08 作者: 遠(yuǎn)地點(diǎn) 時(shí)間: 2025-3-27 17:58 作者: PLAYS 時(shí)間: 2025-3-28 00:31 作者: 創(chuàng)新 時(shí)間: 2025-3-28 02:57 作者: Flatus 時(shí)間: 2025-3-28 09:36
Lecture Notes in Electrical Engineeringe usually low and de-conjugation during sample preparation often occurs. In this chapter we describe a method that can be adapted to different model organisms, and substrates to detect SUMOylation. We focus on cohesin and show that SUMOylation indeed occurs in most of the subunits of budding yeast cohesin.作者: 沉積物 時(shí)間: 2025-3-28 14:29
Ruzena Bajcsy,Eric Krotkov,Max Mintzhigher eukaryotes, in DNA repair in human cells. In this review, we briefly describe what we know about cohesin function and regulation in response to DNA damage, and discuss the optimized laser microirradiation conditions used to analyze cohesin responses to DNA damage in vivo.作者: indemnify 時(shí)間: 2025-3-28 15:05
Detection of Cohesin SUMOylation In Vivoe usually low and de-conjugation during sample preparation often occurs. In this chapter we describe a method that can be adapted to different model organisms, and substrates to detect SUMOylation. We focus on cohesin and show that SUMOylation indeed occurs in most of the subunits of budding yeast cohesin.作者: Keshan-disease 時(shí)間: 2025-3-28 19:34
The Use of Laser Microirradiation to Investigate the Roles of Cohesins in DNA Repairhigher eukaryotes, in DNA repair in human cells. In this review, we briefly describe what we know about cohesin function and regulation in response to DNA damage, and discuss the optimized laser microirradiation conditions used to analyze cohesin responses to DNA damage in vivo.作者: PACT 時(shí)間: 2025-3-29 00:01 作者: COKE 時(shí)間: 2025-3-29 05:47 作者: intelligible 時(shí)間: 2025-3-29 10:57 作者: Hearten 時(shí)間: 2025-3-29 12:29
Using Fluorescent Reporters in Conjunction with Cytometry and Statistics to Assess Nuclear Accumulatproteins using cytometry and biostatistics. This assay was developed for use with GFP reporters for ribosome biogenesis in budding yeast but could be adapted for use with any GFP reporter that accumulates into the nucleus under adverse conditions.作者: languor 時(shí)間: 2025-3-29 16:23
https://doi.org/10.1007/978-94-007-1324-6d chromosomes using egg extracts, along with cytological and biochemical assays for addressing the molecular mechanisms of sister chromatid cohesion. A powerful approach involving immunodepletion of cohesin and its regulators is also included.作者: Sedative 時(shí)間: 2025-3-29 20:08 作者: scotoma 時(shí)間: 2025-3-30 00:33
Laura Landi,Cinzia Tamburini,Aldo Romaniohesin is actually loaded onto chromatin. Further, Nipbl seems to have also additional roles, for instance as transcription factor..This chapter summarizes our current knowledge on kollerin function and the recent studies on the genomic localization of Scc2, highlighting and critically discussing controversial data.作者: 模仿 時(shí)間: 2025-3-30 06:17
Ruzena Bajcsy,Eric Krotkov,Max Mintzscently labeled positions on the same chromosome. In combination with an automated computational analysis pipeline, this assay enables the study of various candidate proteins for their roles in regulating genome topology during cell divisions.作者: brachial-plexus 時(shí)間: 2025-3-30 09:54 作者: 一加就噴出 時(shí)間: 2025-3-30 12:56
Book 2017and function of cohesin and the related factor condensin in vitro and in vivo across different model organisms, as well as in human cells. .Cohesin and Condensin: Methods and Protocols .is divided into three parts: Part I explores various in vitro and in vivo systems used to study the fundamental me作者: 歡笑 時(shí)間: 2025-3-30 17:30 作者: 訓(xùn)誡 時(shí)間: 2025-3-30 21:42
Biochemical and Functional Assays of Human Cohesin-Releasing Factor Wapln from chromosomes during both interphase and mitosis. Here, we describe in vitro protein binding assays between Wapl and a cohesin subcomplex, and cellular assays in human cells that probe the functions of Wapl in cohesin release.作者: fleeting 時(shí)間: 2025-3-31 02:58 作者: 嫻熟 時(shí)間: 2025-3-31 08:03
A Protocol for Measuring Mitotic Chromosome Condensation Quantitatively in Fission Yeast Cellsscently labeled positions on the same chromosome. In combination with an automated computational analysis pipeline, this assay enables the study of various candidate proteins for their roles in regulating genome topology during cell divisions.作者: 掃興 時(shí)間: 2025-3-31 13:15 作者: 異端邪說2 時(shí)間: 2025-3-31 14:41
A Sister Chromatid Cohesion Assay Using , Egg Extractsm chromatin is incubated with these extracts, it is first duplicated within an assembled nucleus, and is then transformed into mitotic chromosomes, in each of which sister chromatids are juxtaposed with each other in a cohesin-dependent manner. Here we describe our protocols for assembling duplicate作者: 顛簸地移動 時(shí)間: 2025-3-31 18:04
An In Vitro Assay for Monitoring Topological DNA Entrapment by the Chromosomal Cohesin Complex. Cohesin is a large, ring-shaped protein complex and is thought to entrap DNA molecules inside of its ring. The unique DNA association is central to cohesin function and requires its ATPase and another heterodimer complex called the cohesin loader. Here we describe the biochemical reconstitution of
