標(biāo)題: Titlebook: Clinical Applications of PCR; Y. M. Dennis Lo Book 1998 Humana Press 1998 [打印本頁] 作者: 專家 時間: 2025-3-21 17:58
書目名稱Clinical Applications of PCR影響因子(影響力)
書目名稱Clinical Applications of PCR影響因子(影響力)學(xué)科排名
書目名稱Clinical Applications of PCR網(wǎng)絡(luò)公開度
書目名稱Clinical Applications of PCR網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Clinical Applications of PCR被引頻次
書目名稱Clinical Applications of PCR被引頻次學(xué)科排名
書目名稱Clinical Applications of PCR年度引用
書目名稱Clinical Applications of PCR年度引用學(xué)科排名
書目名稱Clinical Applications of PCR讀者反饋
書目名稱Clinical Applications of PCR讀者反饋學(xué)科排名
作者: Dungeon 時間: 2025-3-22 00:04
https://doi.org/10.1007/978-3-031-61231-2 (Perkin Elmer, Applied Biosystems Division [PE-ABD], Foster City, CA), which can detect four different fluorescent colors in the same lane without controlling gel-temperature. We designed and attached a gel temperature-controlling system to ABI373 A and developed a sensitive method of multiple fluo作者: TRAWL 時間: 2025-3-22 03:43 作者: 帶來墨水 時間: 2025-3-22 07:57
Risks in the Conversion of Energy,ll or organelle. In many cases the level of mutant in an affected tissue correlates well with disease severity. Ideally, a screening test to detect a pathogenic point mutation should not only identify the presence or absence of a pathogenic mutation, but also quantitate the level of the mutation com作者: 預(yù)測 時間: 2025-3-22 11:45
1543-1894 new to molecular diagnosis. The next chapter includes useful tips for setting up a PCR laboratory. Section 2 then outlines some of the most commonly used PCR-ba978-0-89603-359-7978-1-59259-600-3Series ISSN 1543-1894 Series E-ISSN 1940-6037 作者: 奇思怪想 時間: 2025-3-22 14:16
Multiple Fluorescence-Based PCR-SSCP Analysis with Primer-, Post-, and Internal-Labeling (Perkin Elmer, Applied Biosystems Division [PE-ABD], Foster City, CA), which can detect four different fluorescent colors in the same lane without controlling gel-temperature. We designed and attached a gel temperature-controlling system to ABI373 A and developed a sensitive method of multiple fluo作者: 奇思怪想 時間: 2025-3-22 17:06 作者: 個人長篇演說 時間: 2025-3-23 01:15
Diagnosis of Mitochondrial Disorders Using the PCRll or organelle. In many cases the level of mutant in an affected tissue correlates well with disease severity. Ideally, a screening test to detect a pathogenic point mutation should not only identify the presence or absence of a pathogenic mutation, but also quantitate the level of the mutation com作者: misshapen 時間: 2025-3-23 04:24
Risks of Harm from Psychopathic Individualssed into the blood stream as a result of surgery. Detection of circulating tumor cells at diagnosis in early stage patients would imply that the disease is more advanced than indicated by conventional staging. These patients might then be offered adjuvant chemotherapy or radiotherapy post surgery.作者: 生命層 時間: 2025-3-23 06:44 作者: 同義聯(lián)想法 時間: 2025-3-23 13:34 作者: 草本植物 時間: 2025-3-23 15:43
Community Knowledge About Security:oidance is therefore the single most important consideration when setting up a PCR laboratory, especially one concerned with generating diagnostic information. In many situations, precautions which are normally taken in the handling of microbiological materials are equally applicable to PCR-related procedures.作者: 暫時別動 時間: 2025-3-23 18:50 作者: INCUR 時間: 2025-3-23 23:47
Blockchain Olive Oil Supply Chain,tly there is a move to use nonisotopic labels, such as biotin and digoxigenin. Nonisotopic probes also have the advantage of much better stability over time compared with isotopic probes that have limited half-lives.作者: 喃喃訴苦 時間: 2025-3-24 05:43
Domenico Geraci,Leonard Sassanod in the broad area of research applications in gene expression studies. Since its introduction (.,.) the method is constantly finding new applications, especially in projects aimed at detecting and cloning differentially expressed genes (.,.).作者: Sleep-Paralysis 時間: 2025-3-24 07:14 作者: ALIBI 時間: 2025-3-24 14:38
Mutation Screening Using PCR-SSCPlly developed by Orita et al. (.). It has the ability of detecting a single base change, and has been applied to a number of genes, including the insulin receptor (.), GLUT 4 (.), glucokinase (.), and the mitochondrial genome (.).作者: anthropologist 時間: 2025-3-24 17:57
Generation of Labeled Probes by PCRtly there is a move to use nonisotopic labels, such as biotin and digoxigenin. Nonisotopic probes also have the advantage of much better stability over time compared with isotopic probes that have limited half-lives.作者: Hiatal-Hernia 時間: 2025-3-24 22:41
Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)d in the broad area of research applications in gene expression studies. Since its introduction (.,.) the method is constantly finding new applications, especially in projects aimed at detecting and cloning differentially expressed genes (.,.).作者: Missile 時間: 2025-3-24 23:33
Noureddine Amraoui,Belhassen Zouarin factor makes PCR very sensitive to small variations of the experimental conditions; the second problem is caused by the saturation phenomenon, i.e., the gradual decrease of the amplification efficiency that starts in the later stages of PCR, usually following the accumulation of some threshold amount of product.作者: predict 時間: 2025-3-25 04:57
Salah Gontara,Amine Boufaied,Ouajdi Korbaawing bone marrow transplantation (. Chapter 15) and noninvasive prenatal diagnosis (. Chapters 21 and 22). This chapter reviews the theoretical and practical considerations of minority DNA population detection by PCR.作者: 尖酸一點 時間: 2025-3-25 11:29
Violence and Criminal Psychopathy,. In the longterm this may lead to improved clinical outcome by additional treament of persistent disease following conventional therapy or early intervention in patients with micrometastatic disease and preclinical relapse.作者: 共同給與 時間: 2025-3-25 14:47 作者: glomeruli 時間: 2025-3-25 16:30
PCR for the Detection of Minority DNA Populationswing bone marrow transplantation (. Chapter 15) and noninvasive prenatal diagnosis (. Chapters 21 and 22). This chapter reviews the theoretical and practical considerations of minority DNA population detection by PCR.作者: reptile 時間: 2025-3-25 21:16
Detection of Circulating Solid Tumor Cells by Reverse Transcriptase Polymerase Chain Reaction. In the longterm this may lead to improved clinical outcome by additional treament of persistent disease following conventional therapy or early intervention in patients with micrometastatic disease and preclinical relapse.作者: Carminative 時間: 2025-3-26 02:52
Julien Bringer,Hervé Chabanne,Linda Guigaity and speed of PCR have revolutionized molecular diagnostics, allowmg the realization of a number of applications that were impossible in the pre-PCR era. This chapter offers an mtroductory guide to the process.作者: declamation 時間: 2025-3-26 05:51
Akram Idani,Yves Ledru,German Vegasite-directed mutagenesis using primers with mismatches near the 3′ ends, it is possible to create an artificial restriction fragment length polymorphism (A-RFLP) for almost all naturally occurring DNA polymorphisms .–.. . illustrates the principles of this approach.作者: 捕鯨魚叉 時間: 2025-3-26 09:12 作者: 槍支 時間: 2025-3-26 15:43
,How to Develop a “Safe Society”,ved after BMT and the relationships between mixed chimerism, engraftment and relapse have remained controversial (.–.). To evaluate the clinical relevance of mixed chimerism after BMT, we developed a method based on the amplification of minisatellite DNA regions by the polymerase chain reaction (PCR) (.,.).作者: Pathogen 時間: 2025-3-26 19:18
Introduction to the Polymerase Chain Reactionity and speed of PCR have revolutionized molecular diagnostics, allowmg the realization of a number of applications that were impossible in the pre-PCR era. This chapter offers an mtroductory guide to the process.作者: GRIEF 時間: 2025-3-26 21:02 作者: Projection 時間: 2025-3-27 01:58
Long Range PCRThe . polymerase lacks proofreading properties (.) and thus is unable to correct such misincorporations. The higher extension K. value for a misincorporated nucleotide is thought to cause detachment of the . polymerase from template DNA.作者: 外來 時間: 2025-3-27 07:27 作者: 陳列 時間: 2025-3-27 09:46 作者: DEI 時間: 2025-3-27 14:56 作者: Nefarious 時間: 2025-3-27 19:39 作者: –DOX 時間: 2025-3-28 01:55
Sequencing of PCR ProductsIt is often necessary to obtain DNA sequence information following successful amplification. Although polymerase chain reaction (PCR) products may be cloned into a vector and sequenced at a later stage, it is often preferable to sequence PCR products directly, without subcloning, and it is this approach that is addressed in this chapter.作者: 不怕任性 時間: 2025-3-28 04:41 作者: antedate 時間: 2025-3-28 08:48
Amplificationtissue specimens. . hybrrdization (ISH) does permit localization of specific nucleic acid sequences at the individual cell level. In conventional nonisotopic . (IS) detection systems, most protocols do not detect single copy genes, except for those incorporating elaborate sandwich detection techniques as described by Herrington et al. (.).作者: 概觀 時間: 2025-3-28 11:54
PCR Analysis of CD44 Variants in Tumorshain disulfide bonds. It is essentially composed of a 37-kDa protein core highly glycosylated by .- and .-linked sugars to form an 85–90-kDa product and is sometimes additionally linked to chondroitin sulfate side chains to produce a 180–220 kDa form on sodium dodecyl sulfate (SDS) gels (.,.).作者: essential-fats 時間: 2025-3-28 17:25
Clinical Applications of PCR978-1-59259-600-3Series ISSN 1543-1894 Series E-ISSN 1940-6037 作者: 青石板 時間: 2025-3-28 21:11 作者: 不能平靜 時間: 2025-3-28 23:25
https://doi.org/10.1007/978-981-10-6271-1tissue specimens. . hybrrdization (ISH) does permit localization of specific nucleic acid sequences at the individual cell level. In conventional nonisotopic . (IS) detection systems, most protocols do not detect single copy genes, except for those incorporating elaborate sandwich detection techniques as described by Herrington et al. (.).作者: 喪失 時間: 2025-3-29 05:43 作者: Nostalgia 時間: 2025-3-29 08:46
Julien Bringer,Hervé Chabanne,Linda Guigaegantly simple but the resulting method is extremely powerful. The adoption of the thermostable . polymerase in 1988 greatly simplifies the process and enables the automatron of PCR (.). Since then a large number of applications have been developed that are based on the basic PCR theme. The versatil作者: CLIFF 時間: 2025-3-29 12:53
Community Knowledge About Security:ty has also given PCR its main weakness, namely, its tendency to produce false-positive results due to exogenous contamination (.,.). Contamination avoidance is therefore the single most important consideration when setting up a PCR laboratory, especially one concerned with generating diagnostic inf作者: micronutrients 時間: 2025-3-29 15:52
Community Knowledge About Security:amplify nucleic acid sequences from archival materials, which in many institutions consist of paraffin-embedded tissue samples (.,.). This ability has allowed the carrying out of large scale retrospective studies of archival materials and has facilitated the use of materials from multiple institutio作者: 懶洋洋 時間: 2025-3-29 19:56 作者: MULTI 時間: 2025-3-30 02:24
Lecture Notes in Computer Scienceetecting mutations as single-stranded conformational polymorphisms (SSCP) is a convenient method of screening for possible mutations. SSCP was originally developed by Orita et al. (.). It has the ability of detecting a single base change, and has been applied to a number of genes, including the insu作者: 易怒 時間: 2025-3-30 05:56
https://doi.org/10.1007/978-3-031-61231-2ding even one point mutation. A disadvantage of PCR-SSCP, despite its sensitivity, is the necessity to use radioisotopes. To avoid radioisotopes, silver staining was introduced for band detection (.). In the fluorescent amplification refractory mutation system (ARMS)-SSCP using two different fluores作者: albuminuria 時間: 2025-3-30 10:24
Risks and Security of Internet and Systems a way that it is able to discriminate among templates that differ by a single nucleotide residue (.),.) ARMS has also been termed allele-specific PCR (.) or PCR amplification of specific alleles (PASA) (.). Thus, an ARMS primer can be designed to amplify a specific member of a multi-allelic system 作者: 值得贊賞 時間: 2025-3-30 14:46 作者: 背景 時間: 2025-3-30 18:41
Risks and Security of Internet and Systems λ DNA to be amplified (.). The maximum amplifiable length of PCR is limited by the low fidelity of the . (.) DNA polymerase (.), the most commonly used thermostable polymerase. It is believed that inadvertent nucleotide misincorporations during the PCR extension steps cause chain terminations (.). 作者: 孤獨無助 時間: 2025-3-30 22:59
Blockchain Olive Oil Supply Chain,olecule, which is usually a radioisotope. For diagnostic techniques carried out in a clinical laboratory, radioisotopes are hazardous and, thus, recently there is a move to use nonisotopic labels, such as biotin and digoxigenin. Nonisotopic probes also have the advantage of much better stability ove作者: NUDGE 時間: 2025-3-31 04:21
