標(biāo)題: Titlebook: Circulating Tumor Cells; Methods and Protocol Mark Jesus M. Magbanua,John W. Park Book 2017 Springer Science+Business Media LLC 2017 Cytome [打印本頁(yè)] 作者: 贖罪 時(shí)間: 2025-3-21 17:49
書目名稱Circulating Tumor Cells影響因子(影響力)
作者: unstable-angina 時(shí)間: 2025-3-21 23:00 作者: Nutrient 時(shí)間: 2025-3-22 02:01 作者: spinal-stenosis 時(shí)間: 2025-3-22 08:07
Microfluidic-Based Enrichment and Retrieval of Circulating Tumor Cells for RT-PCR Analysis, enrichment technologies. We have developed a novel device to enrich and retrieve CTCs from blood samples by using a microfluidic chip. The Celsee PREP100 device captures CTCs with high sensitivity and allows the captured CTCs to be retrieved for molecular analysis. It uses the microfluidic chip whi作者: Acetabulum 時(shí)間: 2025-3-22 12:14
Microscale Laminar Vortices for High-Purity Extraction and Release of Circulating Tumor Cells,nable noninvasive tumor profiling, treatment monitoring, and identification of targeted treatments. Accurate and rapid purification of CTCs holds great potential to improve cancer care but the task remains technically challenging. Microfluidic isolation of CTCs within microscale vortices enables hig作者: 注入 時(shí)間: 2025-3-22 13:22 作者: 注入 時(shí)間: 2025-3-22 19:25 作者: meritorious 時(shí)間: 2025-3-22 21:24
Fourier Ptychographic Microscopy for Rapid, High-Resolution Imaging of Circulating Tumor Cells Enridue to the development of technologies aimed at the enrichment of circulating tumor cells (CTCs), the subpopulation of primary tumor cells that gain access to the circulatory system and are responsible for colonization at distant sites. In contrast to other technologies, filtration-based CTC enrichm作者: 殺子女者 時(shí)間: 2025-3-23 03:24 作者: BARB 時(shí)間: 2025-3-23 05:48 作者: COM 時(shí)間: 2025-3-23 11:24 作者: chalice 時(shí)間: 2025-3-23 17:52 作者: 意外 時(shí)間: 2025-3-23 20:22 作者: 惡名聲 時(shí)間: 2025-3-24 00:16 作者: 地殼 時(shí)間: 2025-3-24 03:01 作者: 護(hù)身符 時(shí)間: 2025-3-24 10:02 作者: 強(qiáng)行引入 時(shí)間: 2025-3-24 12:03 作者: ATP861 時(shí)間: 2025-3-24 16:45
Flow Cytometric Detection of Circulating Tumor Cells Using a Candidate Stem Cell Marker, p75 Neurott using antibodies against epithelial cell adhesion molecule (EpCAM), which is overexpressed in epithelial cells. A neurotrophin receptor p75 (p75NTR) is expressed in a candidate stem cell fraction in esophageal squamous cell carcinoma (ESCC), which shows significantly higher colony formation, enhan作者: 描述 時(shí)間: 2025-3-24 19:53 作者: 多產(chǎn)子 時(shí)間: 2025-3-25 02:57 作者: painkillers 時(shí)間: 2025-3-25 03:26 作者: 冷漠 時(shí)間: 2025-3-25 09:42
1064-3745 ation advice from the experts.This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Each chapter provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included ar作者: 蜿蜒而流 時(shí)間: 2025-3-25 13:34 作者: conference 時(shí)間: 2025-3-25 19:27
https://doi.org/10.1007/978-1-4684-5395-9racteristics and properties. Microfluidics presents a unique platform to isolate and study CTCs. In this chapter, we describe the detailed procedures for the fabrication and use of a microfluidic device to detect CTCs from the blood of pancreatic cancer patients.作者: Gyrate 時(shí)間: 2025-3-25 22:12
R. C. Mühlbauer,J.-P. Bonjour,H. Fleischysical properties (size and deformability). Viable circulating tumor cells can be successfully enriched and analyzed, or easily released for further characterization due to the novel separable two-layer design.作者: Melodrama 時(shí)間: 2025-3-26 00:12 作者: Outspoken 時(shí)間: 2025-3-26 04:23 作者: Matrimony 時(shí)間: 2025-3-26 10:26 作者: Cloudburst 時(shí)間: 2025-3-26 14:24
Separable Bilayer Microfiltration Device for Label-Free Enrichment of Viable Circulating Tumor Cellysical properties (size and deformability). Viable circulating tumor cells can be successfully enriched and analyzed, or easily released for further characterization due to the novel separable two-layer design.作者: 鐵砧 時(shí)間: 2025-3-26 19:03 作者: 冰河期 時(shí)間: 2025-3-26 23:58
Book 2017 provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included are protocols for capture of CTCs via filtration and density gradient centrifugation; microfluidic and immunomagnetic separation of CTCs; detection of CTCs by immunocytochemistry, f作者: 出生 時(shí)間: 2025-3-27 04:57 作者: Inculcate 時(shí)間: 2025-3-27 08:12 作者: 鋼筆記下懲罰 時(shí)間: 2025-3-27 11:57 作者: 誘騙 時(shí)間: 2025-3-27 15:35 作者: commute 時(shí)間: 2025-3-27 21:42 作者: jaundiced 時(shí)間: 2025-3-27 22:09 作者: 首創(chuàng)精神 時(shí)間: 2025-3-28 03:23
Hiroaki Kataoka,Tsuyoshi Fukushima two steps: (1) EPCAM-based immunomagnetic enrichment followed by (2) flow cytometry (IE/FC). The assay takes approximately 2?h to complete. In addition to tumor cell enumeration, IE/FC offers opportunities for direct isolation of highly pure tumor cells for downstream molecular characterization.作者: Lipohypertrophy 時(shí)間: 2025-3-28 10:09
https://doi.org/10.1007/978-1-4684-6003-2ced tumor formation in mice, along with strong expression of epithelial mesenchymal transition-related genes. Here, we describe a method to detect CTCs in ESCC based on the combined expression of EpCAM and p75NTR using flow cytometry, demonstrating the feasibility of expression analysis of multiple cell surface markers in viable cells.作者: Bucket 時(shí)間: 2025-3-28 14:17 作者: 憤怒事實(shí) 時(shí)間: 2025-3-28 15:05 作者: NUL 時(shí)間: 2025-3-28 22:37 作者: medieval 時(shí)間: 2025-3-29 02:44
Capture and Genetic Analysis of Circulating Tumor Cells Using a Magnetic Separation Device (Magnetimethod allows rapid isolation of CTCs and subsequent integration with downstream genetic profiling methods for clinical applications such as targeted therapy, therapy monitoring, or further biological studies.作者: ANTE 時(shí)間: 2025-3-29 06:33 作者: 轎車 時(shí)間: 2025-3-29 09:33 作者: Torrid 時(shí)間: 2025-3-29 15:19 作者: 厭惡 時(shí)間: 2025-3-29 17:12
Maryse A. Block,Eric Maréchal,Jacques Joyardtegrated with a microfiltration-based CTC and rare cell enrichment device we have previously described, we present a protocol for Fourier Ptychographic Microscopy (FPM), a method that, unlike many automated imaging platforms, produces high-speed, high-resolution images that can be digitally refocuse作者: Inferior 時(shí)間: 2025-3-29 23:00
The Differentiation of Male Germ Cells, from a blood sample, and the spreading of these nucleated cells onto glass slides permitting immunofluorescent staining, cell identification, and individual cell picking described in subsequent chapters. In addition to collection of rare cells such as CTCs, AccuCyte also collects cells of the circu作者: 同義聯(lián)想法 時(shí)間: 2025-3-30 00:00 作者: 不知疲倦 時(shí)間: 2025-3-30 08:06 作者: 思想上升 時(shí)間: 2025-3-30 10:44 作者: obscurity 時(shí)間: 2025-3-30 12:48
Fourier Ptychographic Microscopy for Rapid, High-Resolution Imaging of Circulating Tumor Cells Enritegrated with a microfiltration-based CTC and rare cell enrichment device we have previously described, we present a protocol for Fourier Ptychographic Microscopy (FPM), a method that, unlike many automated imaging platforms, produces high-speed, high-resolution images that can be digitally refocuse作者: 熱心 時(shí)間: 2025-3-30 19:37 作者: precede 時(shí)間: 2025-3-30 23:33
Multispectral Imaging Analysis of Circulating Tumor Cells in Negatively Enriched Peripheral Blood S, this application uses a previously developed, technology that performs a purely negative enrichment methodology on peripheral blood, yielding highly enriched blood samples that contain CTCs as well as other, undefined cell types. The focus of this contribution is the use of multispectral imaging o作者: insightful 時(shí)間: 2025-3-31 04:21
Book 2017known pitfalls..Cutting-edge and authoritative, .Circula.ting Tumor Cells: Methods and Protocols?is a valuable resource for laboratory researchers and clinicians who are interested in furthering their studies on CTCs..作者: fallible 時(shí)間: 2025-3-31 05:26
Edward E. Wallach,Susan J. Atlaspter is to describe the use of the 4-channel CMx (cells captured in maximum) microfluidic chip, containing special micropatterns coated with an antibody-conjugated supported lipid bilayer (SLB) on its surface, to capture and isolate CTCs from the blood of cancer patients. Captured CTCs are subsequen作者: Hiatal-Hernia 時(shí)間: 2025-3-31 12:26
A. Negro-Vilar,M. M. Valenca,M. D. Culleric and diagnostic values. Here, we describe a procedure for separating CTCs from whole blood based on size and deformability using the microfluidic ratchet device. This device leverages the ratcheting motion of single cells created as they are deformed through funnel-shaped constrictions using oscil作者: 抵消 時(shí)間: 2025-3-31 15:58
https://doi.org/10.1007/978-1-4684-5395-9ogression. Analysis of these rare malignant cells has the potential to further our understanding of cancer metastasis by gaining insights into CTC characteristics and properties. Microfluidics presents a unique platform to isolate and study CTCs. In this chapter, we describe the detailed procedures 作者: ALE 時(shí)間: 2025-3-31 17:31 作者: Missile 時(shí)間: 2025-3-31 23:59 作者: 決定性 時(shí)間: 2025-4-1 03:32
R. C. Mühlbauer,J.-P. Bonjour,H. Fleisch, and the mechanism of cancer metastasis. Here we describe in detail a label-free enrichment process of circulating tumor cells based on its unique physical properties (size and deformability). Viable circulating tumor cells can be successfully enriched and analyzed, or easily released for further c作者: considerable 時(shí)間: 2025-4-1 08:54
https://doi.org/10.1007/0-306-48148-0ore geometry reduces the shear stress that the cells would typically experience during filtration process and allows the cells to remain viable. The microfilter provides a platform capable of high CTC capture efficiency; however, the release of these cells from the filter following capture is nontri作者: 哄騙 時(shí)間: 2025-4-1 10:11
Maryse A. Block,Eric Maréchal,Jacques Joyarddue to the development of technologies aimed at the enrichment of circulating tumor cells (CTCs), the subpopulation of primary tumor cells that gain access to the circulatory system and are responsible for colonization at distant sites. In contrast to other technologies, filtration-based CTC enrichm作者: 沖擊力 時(shí)間: 2025-4-1 16:53
W. R. Bartle,V. Braun,N. E. Winsladecell types, including circulating tumor cells (CTCs), captured on the filter membrane can be simultaneously identified via immunocytochemistry (ICC) analysis of specific cellular biomarkers. Here, we describe an automated microfluidic filtration method combined with a liquid handling system for sequ作者: Celiac-Plexus 時(shí)間: 2025-4-1 22:21
Jonathan B. Meddings,John M. Dietschyr gene-amplifications and screen cancer patients eligible for targeted therapies. As the number of CTCs is a critical parameter to identify such biomarkers, we developed fluorescent in situ hybridization (FISH) for CTCs enriched on filters (filter-adapted-FISH, FA-FISH). Here, we describe the FA-FIS作者: Resign 時(shí)間: 2025-4-1 23:52
https://doi.org/10.1007/978-94-011-4818-4tate, colon, and lung cancers. Higher numbers of these cells in blood are associated with a poorer prognosis of patients. Genomic profiling of CTCs would help characterize markers specific for the identification of these cells in blood, and also define genomic alterations that give these cells a met
