標(biāo)題: Titlebook: Circular RNAs; Methods and Protocol Christoph Dieterich,Argyris Papantonis Book 2018 Springer Science+Business Media, LLC, part of Springer [打印本頁] 作者: CLOG 時間: 2025-3-21 18:56
書目名稱Circular RNAs影響因子(影響力)
書目名稱Circular RNAs影響因子(影響力)學(xué)科排名
書目名稱Circular RNAs網(wǎng)絡(luò)公開度
書目名稱Circular RNAs網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Circular RNAs被引頻次
書目名稱Circular RNAs被引頻次學(xué)科排名
書目名稱Circular RNAs年度引用
書目名稱Circular RNAs年度引用學(xué)科排名
書目名稱Circular RNAs讀者反饋
書目名稱Circular RNAs讀者反饋學(xué)科排名
作者: hallow 時間: 2025-3-21 23:24
978-1-4939-8521-0Springer Science+Business Media, LLC, part of Springer Nature 2018作者: Alopecia-Areata 時間: 2025-3-22 04:19
Circular RNAs978-1-4939-7562-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 有權(quán)威 時間: 2025-3-22 06:44 作者: Infirm 時間: 2025-3-22 09:53 作者: 斜 時間: 2025-3-22 16:30
https://doi.org/10.1007/978-3-642-54508-5t the 5′ end is first prepared by a chemical or enzymatic method, then circularized using ligase. The function of the prepared circular RNA molecule, such as an ability to induce translation, can then be investigated.作者: 斜 時間: 2025-3-22 17:57
Characterization and Validation of Circular RNA and Their Host Gene mRNA Expression Using PCR,plice site, being the unique sequence element of a circular RNA, enables the detection of circular RNA expression. Here we describe the basic techniques to detect different circular transcripts of a gene or one circular RNA specifically by PCR and highlight the advantages and drawbacks of both.作者: 滲透 時間: 2025-3-23 00:37
Nonradioactive Northern Blot of circRNAs,y to confirm the existence and to evaluate the size of individual circRNAs. Northern blotting probes can be radioactive isotope (.P) labeled, which is not environment-friendly and sometimes inconvenient to use. Here, we describe a nonradioactive northern blot protocol with digoxigenin-labeled probe to detect circRNA.作者: mortgage 時間: 2025-3-23 04:43
Preparation of Circular RNA In Vitro,t the 5′ end is first prepared by a chemical or enzymatic method, then circularized using ligase. The function of the prepared circular RNA molecule, such as an ability to induce translation, can then be investigated.作者: Affirm 時間: 2025-3-23 07:15
Christoph Dieterich,Argyris PapantonisIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 蓋他為秘密 時間: 2025-3-23 09:41 作者: 內(nèi)部 時間: 2025-3-23 14:59 作者: poliosis 時間: 2025-3-23 20:52 作者: 滴注 時間: 2025-3-24 01:28 作者: 思想流動 時間: 2025-3-24 05:49
Effective Supervision and Enforcement,of free ends makes them very stable compared to their counterpart linear RNAs. By selectively interacting with microRNAs and RNA-binding proteins (RBPs), circRNAs have been shown to influence gene expression programs. We designed a web tool, CircInteractome, in order to (1) explore potential interac作者: 洞察力 時間: 2025-3-24 07:28 作者: 束縛 時間: 2025-3-24 14:42
Non-fungible Tokens and Stateless Firms,. However, the detection of circular RNAs (circRNAs) using microscopy is challenging because the only feature of a circRNA that can be used for the probe design is its junction. Circular RNAs are expressed at varying levels, and for their efficient monitoring by FISH, background fluorescence levels 作者: emission 時間: 2025-3-24 15:05 作者: Ligneous 時間: 2025-3-24 20:04
https://doi.org/10.1007/978-3-642-54508-5erse roles in cell development, growth, and tumorigenesis, but functions of the majority of circRNAs remain enigmatic. In order to investigate circRNA function it is necessary to manipulate its expression. While various standard approaches exist for circRNA knockdown, here we present cloning vectors作者: pancreas 時間: 2025-3-25 01:49 作者: 槍支 時間: 2025-3-25 04:38 作者: 散布 時間: 2025-3-25 10:35
https://doi.org/10.1007/978-3-642-54508-5y to confirm the existence and to evaluate the size of individual circRNAs. Northern blotting probes can be radioactive isotope (.P) labeled, which is not environment-friendly and sometimes inconvenient to use. Here, we describe a nonradioactive northern blot protocol with digoxigenin-labeled probe 作者: metropolitan 時間: 2025-3-25 15:15
https://doi.org/10.1007/978-3-642-54508-5f them exhibit low expression levels but some are extremely abundant. Typically, circRNAs are studied by RT-PCR-based assays, but for certain types of analyses this technique is not suitable. Circular RNA with repetitive exons (circular concatemers) has been observed by us and others when transientl作者: incubus 時間: 2025-3-25 17:23
https://doi.org/10.1007/978-3-642-54508-5n coding potential, currently classified as long noncoding RNAs (lncRNA). A distinct subgroup of lncRNAs is circular RNAs (circRNAs), which can be easily identified by unique junction reads, resulting from their biogenesis. CircRNAs are largely cytosolic and thought to either code for micro-peptides作者: 屈尊 時間: 2025-3-25 21:09 作者: discord 時間: 2025-3-26 00:53 作者: HERE 時間: 2025-3-26 05:43 作者: Acquired 時間: 2025-3-26 09:39
State Regulation: Achieving Ascendancy,As (miRNAs) and inhibit the activity of miRNAs. Alternatively, miRNAs could also directly target circRNAs and regulate the expression of circRNAs. Here we describe the Argonaute2 (AGO2) RNA immunoprecipitation (RIP) and luciferase screening assays to identify the interaction between circRNAs and miR作者: 牲畜欄 時間: 2025-3-26 15:17 作者: neologism 時間: 2025-3-26 19:46 作者: Amplify 時間: 2025-3-26 23:50
Non-fungible Tokens and Stateless Firms,need to be kept low. Here, we describe a FISH protocol coupled to high-precision localizations using a single fluorescently labeled probe spanning the circRNA junction; this allows circRNA detection in mammalian cells with high signal-to-noise ratios.作者: 急性 時間: 2025-3-27 01:27
https://doi.org/10.1007/978-3-642-54508-5f a circular RNA, CDR1as, simultaneously with an unrelated messenger RNA. The protocol can be adapted to circRNAs that coexist with overlapping, noncircular mRNA isoforms produced from the same genetic locus.作者: 預(yù)定 時間: 2025-3-27 06:13
https://doi.org/10.1007/978-3-642-54508-5ern blot methods can be employed to validate specific circRNAs. Different Northern gel and detection systems are introduced, in combination with additional tools for circRNA characterization, such as RNase R and RNase H treatments.作者: 健談的人 時間: 2025-3-27 11:54
https://doi.org/10.1007/978-3-642-54508-5y expressing circRNAs in cells, however techniques and assays to study these species have not been established. Here, this chapter outlines three biochemical assays (RNase R-, RNase H-, and alkaline-treatment) that combined with northern blotting are useful to study circRNAs in general and circular RNA concatemers in particular.作者: 課程 時間: 2025-3-27 13:38
State Regulation: Achieving Ascendancy,NAs. The AGO2 RIP assay evaluates the potential of the interaction between circRNAs and miRNAs. The luciferase screening assay investigates the targeting miRNAs and the detail binding sites for a specific circRNA.作者: Goblet-Cells 時間: 2025-3-27 19:25
1064-3745 ation advice from the experts.This volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico.? Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful .Methods in 作者: sigmoid-colon 時間: 2025-3-27 22:00
https://doi.org/10.1007/978-3-642-54508-5 function it is necessary to manipulate its expression. While various standard approaches exist for circRNA knockdown, here we present cloning vectors for simplifying the laborious process of cloning circRNAs to achieve high-efficiency overexpression in mammalian cell lines.作者: 連接 時間: 2025-3-28 03:31
https://doi.org/10.1007/978-3-642-54508-5 intact GFP gene. This simple reporter system can be adopted to study how different .-elements and .-factors affect circRNA production, and also can serve as a reliable system to measure the activity of IRES-mediated translation. Therefore this system can serve as a platform for mechanistic studies on the circRNA biogenesis and its function.作者: Emmenagogue 時間: 2025-3-28 07:31 作者: 慎重 時間: 2025-3-28 11:30 作者: 系列 時間: 2025-3-28 17:13
1064-3745 ep, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Circular RNAs: Methods and Protocols .aims to useful and informative for further study into this vital field.?.978-1-4939-8521-0978-1-4939-7562-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: CARK 時間: 2025-3-28 19:50 作者: Scleroderma 時間: 2025-3-28 23:07 作者: 良心 時間: 2025-3-29 03:05
Characterization of Circular RNAs (circRNA) Associated with the Translation Machinery,association of a given circRNA (or any transcript of interest) with distinct ribosomal fractions. This method can evaluate?the coding potential of candidate circRNAs (or any transcript of interest) and its association with the translation machinery.作者: CRAB 時間: 2025-3-29 08:10 作者: OATH 時間: 2025-3-29 13:07 作者: Soliloquy 時間: 2025-3-29 16:33
Effective Supervision and Enforcement,-specific circRNAs, (5) explore potential miRNAs interacting with circRNAs, and (6) design specific siRNAs to silence circRNAs. Here, we review the CircInteractome tool and explain recent updates to the site. The database is freely accessible at ..作者: Hormones 時間: 2025-3-29 22:21
Genome-Wide circRNA Profiling from RNA-seq Data,ng how to access and interpret circRNA annotations. Next, we cover converting circRNA annotations into junction sequences that are used as scaffolds to align RNA-seq reads. Lastly, we visit quantifying circRNA expression trends from the alignment data.作者: Servile 時間: 2025-3-30 01:55
Analysis of Circular RNAs Using the Web Tool CircInteractome,-specific circRNAs, (5) explore potential miRNAs interacting with circRNAs, and (6) design specific siRNAs to silence circRNAs. Here, we review the CircInteractome tool and explain recent updates to the site. The database is freely accessible at ..作者: Initial 時間: 2025-3-30 04:44 作者: Fracture 時間: 2025-3-30 09:03 作者: Metamorphosis 時間: 2025-3-30 14:29 作者: 審問 時間: 2025-3-30 18:47
Detecting Circular RNAs by RNA Fluorescence In Situ Hybridization,need to be kept low. Here, we describe a FISH protocol coupled to high-precision localizations using a single fluorescently labeled probe spanning the circRNA junction; this allows circRNA detection in mammalian cells with high signal-to-noise ratios.作者: DUCE 時間: 2025-3-30 23:13
Single-Molecule Fluorescence In Situ Hybridization (FISH) of Circular RNA CDR1as,f a circular RNA, CDR1as, simultaneously with an unrelated messenger RNA. The protocol can be adapted to circRNAs that coexist with overlapping, noncircular mRNA isoforms produced from the same genetic locus.作者: thyroid-hormone 時間: 2025-3-31 04:26 作者: 考博 時間: 2025-3-31 06:22 作者: HATCH 時間: 2025-3-31 12:25
Identification of circRNAs for miRNA Targets by Argonaute2 RNA Immunoprecipitation and Luciferase SNAs. The AGO2 RIP assay evaluates the potential of the interaction between circRNAs and miRNAs. The luciferase screening assay investigates the targeting miRNAs and the detail binding sites for a specific circRNA.作者: Control-Group 時間: 2025-3-31 14:19
Book 2018ratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Circular RNAs: Methods and Protocols .aims to useful and informative for further study into this vital field.?.
