作者: 符合規(guī)定 時(shí)間: 2025-3-22 00:17 作者: 胖人手藝好 時(shí)間: 2025-3-22 02:30
Mutagenesis in ,otypes, which include dominant or gain-of-function versions of proteins owing to alterations of specific amino acids. This chapter describes the use of EMS mutagenesis in . and how mutagenized plant populations should be handled after the mutagenesis event.作者: DEAF 時(shí)間: 2025-3-22 05:28 作者: NEG 時(shí)間: 2025-3-22 11:46
Wojciech Gagatek,Micha? Kotnarowskisurement and manipulation need to be adopted by circadian biologists. To this end, we describe light measurement in terms of both radiometric and photometric units and consider the appropriate use of light as a stimulus in circadian experiments. In addition, the construction of action spectra has be作者: candle 時(shí)間: 2025-3-22 14:31
Shabnam Sepehri-Boroujeni,Evan Cleave length, and (3) the phase of the circadian activity cycles with respect to the entraining stimulus. Here we describe some of the rationale and the steps required to set up experiments to monitor circadian locomotor activity in .. Suggestions for the statistical analysis of the data obtained in such作者: candle 時(shí)間: 2025-3-22 19:38
Siedlungsstrukturen und Stadtsysteme,otypes, which include dominant or gain-of-function versions of proteins owing to alterations of specific amino acids. This chapter describes the use of EMS mutagenesis in . and how mutagenized plant populations should be handled after the mutagenesis event.作者: 傻瓜 時(shí)間: 2025-3-22 23:48 作者: EWE 時(shí)間: 2025-3-23 02:12
Phasenmodelle der Stadtentwicklung,of reporter gene expression in yeast reporter strains. The method described in this chapter can be used to identify novel protein interactions, analyze protein-protein interactions between two known proteins, as well as dissect interacting protein domains.作者: scrape 時(shí)間: 2025-3-23 07:44 作者: 令人不快 時(shí)間: 2025-3-23 10:46
Yeast Two-Hybrid Screeningof reporter gene expression in yeast reporter strains. The method described in this chapter can be used to identify novel protein interactions, analyze protein-protein interactions between two known proteins, as well as dissect interacting protein domains.作者: Original 時(shí)間: 2025-3-23 14:37
Braulio Gómez Fortes,Laura Cabeza Perezf nonsporulating mycelium and mycelium laden with orange conidia. Assaying . conidiation on race tubes is a simple yet powerful and versatile tool for studying the circadian clock of this model organism.作者: clarify 時(shí)間: 2025-3-23 19:36 作者: Harrowing 時(shí)間: 2025-3-24 01:33 作者: fertilizer 時(shí)間: 2025-3-24 06:24 作者: atopic 時(shí)間: 2025-3-24 07:49 作者: antiandrogen 時(shí)間: 2025-3-24 13:39
https://doi.org/10.1007/3-211-32323-6t approaches to mutagenize the chromosome of ., including insertional mutagenesis, hit-and-run allele replacement, .-mediated gene replacement, and regulated expression of genes from ectopic sites, the neutral sites of the . genome.作者: 文字 時(shí)間: 2025-3-24 18:01
Book 2007 are already active in the field but are interested in learning new techniques and researchers who are considering moving to a new a model system or undertaking comparative studies and would like to consult protocols applied to different organisms before starting the study of new species. This book 作者: Lacerate 時(shí)間: 2025-3-24 21:13 作者: Aprope 時(shí)間: 2025-3-24 23:36
https://doi.org/10.1007/978-1-4613-4080-5expressed genes have been fused to the firefly . gene. Using this reporter gene we have developed a number of automated techniques for monitoring luciferase activity in vivo in plants. This provides us with a robust and high-throughput assay for the circadian clock in ..作者: 自戀 時(shí)間: 2025-3-25 05:44 作者: Prophylaxis 時(shí)間: 2025-3-25 09:07
Phasenmodelle der Stadtentwicklung, an overview of quantitative polymerase chain reaction experiments using the ABI PRISM? 7900HT system for quality control of samples, and micorarray experiments using the Affymetrix GeneChip system for genome-wide expression analysis.作者: Immunotherapy 時(shí)間: 2025-3-25 13:57 作者: 古老 時(shí)間: 2025-3-25 16:42 作者: 哎呦 時(shí)間: 2025-3-25 22:12
Microarrays an overview of quantitative polymerase chain reaction experiments using the ABI PRISM? 7900HT system for quality control of samples, and micorarray experiments using the Affymetrix GeneChip system for genome-wide expression analysis.作者: nostrum 時(shí)間: 2025-3-26 03:45
Rhythmic Conidiation in ,f nonsporulating mycelium and mycelium laden with orange conidia. Assaying . conidiation on race tubes is a simple yet powerful and versatile tool for studying the circadian clock of this model organism.作者: 使激動(dòng) 時(shí)間: 2025-3-26 04:32 作者: 不容置疑 時(shí)間: 2025-3-26 10:07 作者: Hangar 時(shí)間: 2025-3-26 12:40
Detection of Rhythmic Bioluminescence From Luciferase Reporters in Cyanobacteriavectors have been created in our lab for introducing luciferase reporter genes into the . chromosome. A choice of methods and equipment to detect light production from the luciferase fusions provides a means for high-throughput, automated mutant screens as well as testing rhythms from two promoter fusions within the same cell culture.作者: Ondines-curse 時(shí)間: 2025-3-26 19:50 作者: MARS 時(shí)間: 2025-3-26 21:29
Specialized Techniques for Site-Directed Mutagenesis in Cyanobacteriat approaches to mutagenize the chromosome of ., including insertional mutagenesis, hit-and-run allele replacement, .-mediated gene replacement, and regulated expression of genes from ectopic sites, the neutral sites of the . genome.作者: 敏捷 時(shí)間: 2025-3-27 02:50 作者: 極少 時(shí)間: 2025-3-27 09:19
Derek S. Hutcheson,Arjan H. Schakelpared with the known input parameters. Trends are removed; spectra, autocorrelation functions, and rhythmicity indices are produced and discussed. References covering theory and details of all analyses are supplied. All programs employed are available from the author free of charge.作者: modish 時(shí)間: 2025-3-27 09:27
Statistical Analysis of Biological Rhythm Datapared with the known input parameters. Trends are removed; spectra, autocorrelation functions, and rhythmicity indices are produced and discussed. References covering theory and details of all analyses are supplied. All programs employed are available from the author free of charge.作者: deforestation 時(shí)間: 2025-3-27 15:39
Light, Photoreceptors, and Circadian Clocksf the results to emerge are surprising. For example, the rods and cones within the mammalian eye are not required for the alignment (entrainment) of circadian rhythms to the dawn-dusk cycle. There exists a population of directly light-sensitive ganglion cells within the eye that act as brightness de作者: defile 時(shí)間: 2025-3-27 18:18
Statistical Analysis of Biological Rhythm Dataiodicities that is of very high resolution and functions well even in the presence of extreme noise and trend. A method for quantifying the significance, strength, and regularity of the rhythmic process is included. To illustrate these techniques, the author presents analyses of artificial periodic 作者: anthropologist 時(shí)間: 2025-3-27 22:38
Rhythmic Conidiation in ,f agar medium in long growth tubes (so-called “race tubes”), restricting its growth to one direction only, bright orange bands are clearly visible. This banding pattern persists with a periodicity of approx 24 h in the absence of any environmental stimuli. The bands are formed by alternating zones o作者: 紡織品 時(shí)間: 2025-3-28 05:53
Monitoring and Analyzing , Circadian Locomotor Activityarkably rapid advancements in the understanding of the genetic and molecular bases of a complex behavioral trait. To this end, the design of appropriate electronic devices, apt to continuously monitor behavioral activity, has proven to be fundamental to such progress. In particular, most of the muta作者: 啞巴 時(shí)間: 2025-3-28 09:33 作者: wreathe 時(shí)間: 2025-3-28 13:06 作者: nocturia 時(shí)間: 2025-3-28 15:34 作者: LINES 時(shí)間: 2025-3-28 20:16
Analysis of Rhythmic Gene Expression in Adult , Using the Firefly Luciferase Reporter Genected during a specific time of the circadian day need to be sacrificed, followed by the extraction of clock gene products. This procedure makes it impossible to analyze molecular rhythms in an individual over time. To measure clock gene expression within the living animal, firefly luciferase can be 作者: airborne 時(shí)間: 2025-3-29 00:17
Monitoring Circadian Rhythms in , Using Luciferase Reporter Genesnot display any obvious circadian rhythms; however, rhythms in gene expression can be readily detected. For this reason the promoters of rhythmically expressed genes have been fused to the firefly . gene. Using this reporter gene we have developed a number of automated techniques for monitoring luci作者: 微生物 時(shí)間: 2025-3-29 04:03 作者: IOTA 時(shí)間: 2025-3-29 07:13 作者: 帶來(lái) 時(shí)間: 2025-3-29 11:59
Mutagenesis With , . pacemaker genes were discovered through the isolation of gene variants affecting the free-running period of the circadian pacemaker. There are different ways to mutagenize flies. An alkylating agent can be used to randomly alter the fly genome, or transposable elements can be mobilized to disrupt作者: 不滿分子 時(shí)間: 2025-3-29 19:31 作者: Paraplegia 時(shí)間: 2025-3-29 23:40 作者: obviate 時(shí)間: 2025-3-30 03:50
Microarrayshis technology, it is important to perform quality control experiments before engaging in genome-wide expression analysis. In this chapter, we provide an overview of quantitative polymerase chain reaction experiments using the ABI PRISM? 7900HT system for quality control of samples, and micorarray e作者: Fluctuate 時(shí)間: 2025-3-30 04:56 作者: Morsel 時(shí)間: 2025-3-30 09:36
Ezio RosatoMethods for analysis of rhythmic readouts in select model organisms.Novel techniques for identifying clock genes by mutagenesis and mircroarrays.Procedures for isolation, extraction, and analysis of R作者: Repetitions 時(shí)間: 2025-3-30 14:19
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/226574.jpg作者: Bumptious 時(shí)間: 2025-3-30 19:59
https://doi.org/10.1007/978-1-59745-257-1Arabidopsis thaliana; Microarray; PCR; Quantitative PCR; Vivo; Western Blotting; cell lines; cyanobacteria; 作者: SLAY 時(shí)間: 2025-3-30 22:25
Shabnam Sepehri-Boroujeni,Evan CleaveA method is described for measurement of the circadian activity rhythms of up to 150 larval zebrafish simultaneously with a single video image analysis system. Most of the required equipment and software are commercially available, although some components are custom-built.作者: inscribe 時(shí)間: 2025-3-31 02:24
Automated Video Image Analysis of Larval Zebrafish Locomotor RhythmsA method is described for measurement of the circadian activity rhythms of up to 150 larval zebrafish simultaneously with a single video image analysis system. Most of the required equipment and software are commercially available, although some components are custom-built.作者: Abnormal 時(shí)間: 2025-3-31 08:02
Wojciech Gagatek,Micha? Kotnarowskif the results to emerge are surprising. For example, the rods and cones within the mammalian eye are not required for the alignment (entrainment) of circadian rhythms to the dawn-dusk cycle. There exists a population of directly light-sensitive ganglion cells within the eye that act as brightness de作者: anesthesia 時(shí)間: 2025-3-31 11:53
Derek S. Hutcheson,Arjan H. Schakeliodicities that is of very high resolution and functions well even in the presence of extreme noise and trend. A method for quantifying the significance, strength, and regularity of the rhythmic process is included. To illustrate these techniques, the author presents analyses of artificial periodic 作者: 糾纏 時(shí)間: 2025-3-31 16:36 作者: 笨拙的你 時(shí)間: 2025-3-31 19:01
Shabnam Sepehri-Boroujeni,Evan Cleavearkably rapid advancements in the understanding of the genetic and molecular bases of a complex behavioral trait. To this end, the design of appropriate electronic devices, apt to continuously monitor behavioral activity, has proven to be fundamental to such progress. In particular, most of the muta作者: 節(jié)省 時(shí)間: 2025-3-31 22:34 作者: MANIA 時(shí)間: 2025-4-1 02:11 作者: 推崇 時(shí)間: 2025-4-1 07:31 作者: 藝術(shù) 時(shí)間: 2025-4-1 10:50
The pollution dispersion matrix,cted during a specific time of the circadian day need to be sacrificed, followed by the extraction of clock gene products. This procedure makes it impossible to analyze molecular rhythms in an individual over time. To measure clock gene expression within the living animal, firefly luciferase can be
