標(biāo)題: Titlebook: Cilia; Methods and Protocol Vito Mennella Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Sc [打印本頁] 作者: 武士精神 時間: 2025-3-21 19:33
書目名稱Cilia影響因子(影響力)
書目名稱Cilia影響因子(影響力)學(xué)科排名
書目名稱Cilia網(wǎng)絡(luò)公開度
書目名稱Cilia網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cilia被引頻次
書目名稱Cilia被引頻次學(xué)科排名
書目名稱Cilia年度引用
書目名稱Cilia年度引用學(xué)科排名
書目名稱Cilia讀者反饋
書目名稱Cilia讀者反饋學(xué)科排名
作者: colloquial 時間: 2025-3-22 00:02 作者: micturition 時間: 2025-3-22 01:38
Live-Imaging Centriole Amplification in Mouse Brain Multiciliated Cells,loped and optimized over the years to live image the centriole amplification dynamics. We explain why mouse brain MCC is a good model and provide the tips to enable successful spatially and temporally resolved monitoring of this massive organelle reorganization.作者: Mercantile 時間: 2025-3-22 05:36
Affinity Purification of Intraflagellar Transport (IFT) Proteins in Mice Using Endogenous Streptavi for the formation and function of all types of cilia. We describe a method to generate mice with an endogenous C-terminal streptavidin/FLAG tag, using . as an example, and demonstrate the successful implementation of AP in this model. This method can easily be adapted for N- and C-terminal tagging 作者: 疾馳 時間: 2025-3-22 09:00 作者: reserve 時間: 2025-3-22 15:57 作者: reserve 時間: 2025-3-22 19:12
Zeitgeschichte und Politikwissenschafttinct deletions. We describe the use of CRISPR-Cas9 RNP in combination with single-cell RNA sequencing to functionally characterize the impact of gene invalidation in ALI cultures. The complex ecosystem of the airway epithelium, composed of many cell types, makes single-cell approaches particularly 作者: Pedagogy 時間: 2025-3-22 23:09 作者: Flustered 時間: 2025-3-23 05:13
Regierungssystem und Verwaltungspolitikloped and optimized over the years to live image the centriole amplification dynamics. We explain why mouse brain MCC is a good model and provide the tips to enable successful spatially and temporally resolved monitoring of this massive organelle reorganization.作者: Inoperable 時間: 2025-3-23 05:53
Ulrich Widmaier,Andrea Gawrich,Ute Becker for the formation and function of all types of cilia. We describe a method to generate mice with an endogenous C-terminal streptavidin/FLAG tag, using . as an example, and demonstrate the successful implementation of AP in this model. This method can easily be adapted for N- and C-terminal tagging 作者: 以煙熏消毒 時間: 2025-3-23 11:19
Grotz Florian,Ferdinand Müller-Rommelm the murine brain parenchyma and establishing cell cultures of multiciliated ependymal cells from mouse and rat neonatal brain tissue. Our methodology generates a functional readout of ciliary function, specifically high-speed video microscopy (HSVM) enables the quantification of ciliary beat frequ作者: 進(jìn)入 時間: 2025-3-23 16:34 作者: Haphazard 時間: 2025-3-23 21:36 作者: 步兵 時間: 2025-3-24 01:16
Zeitgeschichte und Politikwissenschaftny organisms. It transduces extracellular chemical and mechanical cues to regulate diverse cellular processes during development and physiology. Loss-of-function studies via RNA interference and CRISPR/Cas9-mediated gene knockouts have been the main tool for elucidating the functions of proteins, pr作者: 勉強(qiáng) 時間: 2025-3-24 04:51 作者: Working-Memory 時間: 2025-3-24 09:53 作者: 使腐爛 時間: 2025-3-24 12:38
Die Gauck-Beh?rde: das unm?gliche Amttion and cellular content in three dimensions at nanoscale resolution. Here, we describe the procedure to process and image an air-liquid interface culture of human or mouse airway epithelial cells for visualization of the multiciliated epithelium by SBF-SEM in vertical or horizontal cross section.作者: 持久 時間: 2025-3-24 18:12 作者: fringe 時間: 2025-3-24 20:15 作者: 偏見 時間: 2025-3-25 01:43 作者: 充氣女 時間: 2025-3-25 04:25 作者: CRUMB 時間: 2025-3-25 11:08 作者: Cardiac-Output 時間: 2025-3-25 11:41
Frank Schimmelfennig,Guido Schwellnusy tract. The current chapter details protocols from sample collection to culturing HNEC under air-liquid interface (ALI) conditions to generate well-differentiated, functional airway cultures including displaying beating motile cilia.作者: 壓碎 時間: 2025-3-25 19:00
Regierungssysteme in Mittel- und Osteuropase, and therapies. However, the primary basal cells required to establish the ALI cultures generally lose their ability to differentiate by the second or third passage, requiring a fresh batch, which can be limiting, particularly from donors with rare genotypes or in studies where gene modification 作者: 陰謀小團(tuán)體 時間: 2025-3-25 22:13
Grotz Florian,Ferdinand Müller-Rommelom their apical surface into the cerebrospinal fluid (CSF). This specialized layer of E1 cells constitutes the border between the CSF and the brain interstitial fluid (BIF), and by controlling influx and efflux across the CSF to BIF interface, it is increasingly recognized to play an integral role i作者: 大吃大喝 時間: 2025-3-26 01:11 作者: aptitude 時間: 2025-3-26 07:19
Regierungssysteme in Mittel- und Osteuropa fluid. In cultures, this ciliary beating is not well coordinated or occurs in small focal areas so the resulting mucociliary transport (MCT) is only linear over short distances. We present a method which induces ciliated cells in cultures to align during growth. The cells align along the axis of a 作者: ALLAY 時間: 2025-3-26 11:03
SARS-CoV-2 Infection of Human Primary Nasal Multiciliated Epithelial Cells Grown on Air-Liquid Inteetter understanding respiratory pathogen host-cell interactions in the airways, one approach is to instead grow and differentiate these cells at an air-liquid interface (ALI). This chapter provides the working protocols used in our lab for producing ALI cultures, infecting them with SARS-CoV-2 and monitoring viral replication.作者: 使成整體 時間: 2025-3-26 14:04 作者: 圣人 時間: 2025-3-26 20:18
Endogenous Tagging of Ciliary Genes in Human RPE1 Cells for Live-Cell Imaging,living cells. Here, we describe experimental strategies for endogenous PCR-tagging of ciliary genes in human RPE1 cells and how image acquisition and analysis of the expressed fluorescently tagged proteins can be utilized to study the dynamic ciliary processes of intraflagellar transport and vesicular trafficking.作者: 陳舊 時間: 2025-3-27 00:59
Primary Human Nasal Epithelial Cell Culture,y tract. The current chapter details protocols from sample collection to culturing HNEC under air-liquid interface (ALI) conditions to generate well-differentiated, functional airway cultures including displaying beating motile cilia.作者: 賠償 時間: 2025-3-27 03:48
https://doi.org/10.1007/978-1-0716-3507-0Tissue development; Homeostasis; Ciliopathies; Ciliary proteins; Human disease; Toxicant exposure作者: Arrhythmia 時間: 2025-3-27 08:17
978-1-0716-3509-4The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: 慢慢啃 時間: 2025-3-27 11:35
Vito MennellaIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: Expressly 時間: 2025-3-27 14:47 作者: 誓言 時間: 2025-3-27 19:22
Cilia978-1-0716-3507-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Locale 時間: 2025-3-28 00:42 作者: 即席 時間: 2025-3-28 05:51
Die Gauck-Beh?rde: das unm?gliche Amttion and cellular content in three dimensions at nanoscale resolution. Here, we describe the procedure to process and image an air-liquid interface culture of human or mouse airway epithelial cells for visualization of the multiciliated epithelium by SBF-SEM in vertical or horizontal cross section.作者: IDEAS 時間: 2025-3-28 07:30
Wie gefestigt ist unsere Demokratie?living cells. Here, we describe experimental strategies for endogenous PCR-tagging of ciliary genes in human RPE1 cells and how image acquisition and analysis of the expressed fluorescently tagged proteins can be utilized to study the dynamic ciliary processes of intraflagellar transport and vesicular trafficking.作者: 古老 時間: 2025-3-28 14:04 作者: 表被動 時間: 2025-3-28 17:27 作者: OMIT 時間: 2025-3-28 20:32 作者: ESO 時間: 2025-3-28 23:52 作者: Osteons 時間: 2025-3-29 06:54
Book 2024f different ciliated tissues and their wide applicability in cilia biology. Chapters in this book primarily focus on methods to study multiciliated cells, and discuss topics such as SARS-CoV-2 infections of human primary nasal multiciliated epithelial cells; expansion microscopy of ciliary proteins;作者: antiandrogen 時間: 2025-3-29 11:10 作者: 忘川河 時間: 2025-3-29 14:17 作者: AORTA 時間: 2025-3-29 18:09
Regierungssysteme in Mittel- und Osteuropaodified inserts – referred to as mucociliary transport devices (MCTDs) – are simple to prepare and result reproducibly in a high percentage of cultures demonstrating complete circular transport (CCT).作者: 用手捏 時間: 2025-3-29 22:55
Immunolabel-First-Expand-Later Expansion Microscopy Approach Using Stable STED Dyes,ing their use in an immunolabel-first-expand-later approach. Our protocol overcomes the frequent technical limitation of poor, unreproducible binding of primary antibodies to proteins after denaturation. We demonstrate the applicability of this approach by analyzing both a centriole appendage protein Cep164 and a ciliary protein ARL13B.作者: famine 時間: 2025-3-30 03:29 作者: 縮減了 時間: 2025-3-30 07:04
Mucociliary Transport Device Construction and Application to Study Mucociliary Clearance,odified inserts – referred to as mucociliary transport devices (MCTDs) – are simple to prepare and result reproducibly in a high percentage of cultures demonstrating complete circular transport (CCT).作者: Goblet-Cells 時間: 2025-3-30 11:17 作者: 嬰兒 時間: 2025-3-30 14:19
https://doi.org/10.1007/978-3-663-11275-4n this chapter, we provide a step-by-step protocol describing the process of manual segmentation using 3dMOD within the IMOD package and the process of automated segmentation using Empanada-Napari plugins for the 3D reconstruction of airway cellular structures.作者: REP 時間: 2025-3-30 18:45
Regierungssysteme in Mittel- und Osteuropapassaged more than 20 times retaining their differentiation capacity in ALI cultures. .-transduced basal cells can be frozen and stored long term in liquid nitrogen, enabling transfer of samples between research groups.作者: 土坯 時間: 2025-3-30 22:46
Structural Analysis of Sperm Centrioles Using N-STORM, both the sperm head and tail at the microscale and centriolar substructure details at the nanoscale. This chapter describes the detailed procedures for the selection, preparation, antibody staining, 3D N-STORM imaging, and image quantification of bovine spermatozoa.作者: 減去 時間: 2025-3-31 03:24
Airway Cells 3D Reconstruction via Manual and Machine-Learning Aided Segmentation of Volume EM Datan this chapter, we provide a step-by-step protocol describing the process of manual segmentation using 3dMOD within the IMOD package and the process of automated segmentation using Empanada-Napari plugins for the 3D reconstruction of airway cellular structures.
