標題: Titlebook: Chronic Lymphocytic Leukemia; Methods and Protocol Sami N. Malek Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 20 [打印本頁] 作者: Philanthropist 時間: 2025-3-21 18:04
書目名稱Chronic Lymphocytic Leukemia影響因子(影響力)
書目名稱Chronic Lymphocytic Leukemia影響因子(影響力)學科排名
書目名稱Chronic Lymphocytic Leukemia網(wǎng)絡公開度
書目名稱Chronic Lymphocytic Leukemia網(wǎng)絡公開度學科排名
書目名稱Chronic Lymphocytic Leukemia被引頻次
書目名稱Chronic Lymphocytic Leukemia被引頻次學科排名
書目名稱Chronic Lymphocytic Leukemia年度引用
書目名稱Chronic Lymphocytic Leukemia年度引用學科排名
書目名稱Chronic Lymphocytic Leukemia讀者反饋
書目名稱Chronic Lymphocytic Leukemia讀者反饋學科排名
作者: Gobble 時間: 2025-3-21 21:19
Library of Ethics and Applied Philosophye the first agent is administered to patients and second drug is tested in these patients’ cells in vitro. These assays have been effective in identifying novel agents that work additively or synergistically. In this chapter, we provide a step-by-step protocol for ex vivo drug testing that can be used for combination strategies.作者: Daily-Value 時間: 2025-3-22 01:14
Stage 4: Formulating a Goal Statementhas been employed widely in cytogenetics laboratories worldwide. This chapter describes techniques and trouble-shooting to maximize the efficiency of microscope slide preparation for FISH analysis in CLL.作者: Promotion 時間: 2025-3-22 08:00
https://doi.org/10.1007/978-981-13-9475-1icated DNA with deuterium (.H), a nonradioactive isotope of hydrogen, administered to the patients in drinking water (.H.O) is a safe and reliable method to measure the in vivo birth rates of cells. Here, we describe a protocol to measure chronic lymphocytic leukemia B-cell birth/proliferation and death rates over time using this approach.作者: NORM 時間: 2025-3-22 11:03 作者: 小卒 時間: 2025-3-22 16:28 作者: 小卒 時間: 2025-3-22 18:38 作者: 傷心 時間: 2025-3-22 22:58
https://doi.org/10.1007/978-1-4939-8876-1Mouse modeling; apoptosis; PCR; FISH; Immunoglobulin gene analysis作者: 隱語 時間: 2025-3-23 01:59 作者: 一窩小鳥 時間: 2025-3-23 07:00 作者: Mitigate 時間: 2025-3-23 12:13 作者: 放牧 時間: 2025-3-23 14:10
Stage 4: Formulating a Goal Statementchromosomes microscopically using traditional cytogenetic techniques requires dividing cells to be arrested during metaphase. The major challenge for performing this analysis on CLL samples is stimulating the cells to divide in culture. Stimulation of CLL cells with CpG oligodeoxynucleotides has imp作者: ANT 時間: 2025-3-23 20:23 作者: Allege 時間: 2025-3-24 01:27
https://doi.org/10.1007/978-3-030-99228-6ecombination of IG genes of the heavy chain, followed by VJ recombination of the light chain genes at the pre-B II cell stage. As a result, a fully functional BcR IG is expressed on the surface of any given naive B cell. After antigen encounter, somatic hypermutation (SHM) and class-switch recombina作者: ventilate 時間: 2025-3-24 02:24
Stage 2: Selecting Suitable Goalsthodologies which can be used to identify . mutations in CLL patients; both protocols are primarily intended for research purposes. The functional analysis of separated alleles in yeast (FASAY) can be flexibly adapted to a variable number of samples and provides an immediate functional readout of id作者: 花束 時間: 2025-3-24 10:14
Stage 1: Enhancing Self-Awarenessd CLL harbor these mutations which are typically limited to HEAT domains in the carboxyl-terminus of the protein. Importantly, the mutations are not specific to CLL but also present in several unrelated clonal disorders. Analysis of patient samples and cell lines has shown the primary splicing aberr作者: 高談闊論 時間: 2025-3-24 13:45 作者: Pulmonary-Veins 時間: 2025-3-24 18:40
https://doi.org/10.1057/9781137317193etion assay we described here enables us to evaluate the depletion of CLL cells and monocyte populations upon treatment with drugs targeting the interactions between CLL cells and monocytes. The assay is based on a quantitative multi-color flow cytometry analysis and, when combined to fluorescence-a作者: 含糊其辭 時間: 2025-3-24 21:52
Visuals in the Special Needs Classroomndrial oxidative phosphorylation and extracellular acidification rate which is a measure of glycolysis. Collectively, these assays are used to assess the metabolic phenotype of a cell. Up to four drugs can be loaded and tested in the XF cartridges used in the assay and their effect on cells could be作者: GRIPE 時間: 2025-3-25 02:36 作者: 顧客 時間: 2025-3-25 04:55 作者: 完成 時間: 2025-3-25 10:01
Gretchen Geng,Pamela Smith,Leigh Disneyand molecular profiling of the original tumors, and therefore have been extensively used in cancer research in both the basic and preclinical fields. Here, we describe a PDX model of CLL using autologous activated T cells to support CLL B-cell growth in lymphoid tissues such as spleen and bone marro作者: Indigence 時間: 2025-3-25 15:03
Reflective Practice in Teachingation. Rather they coalesce with numerous “normal” cells of the body and, in the case of CLL, inhabit key immunological niches within secondary lymphoid organs (SLO), where a plethora of stromal and immune cells mediate their growth and survival. With the advent and approval of targeted immune thera作者: 鳥籠 時間: 2025-3-25 16:13 作者: 減少 時間: 2025-3-25 20:50
https://doi.org/10.1007/978-3-030-24505-4n a specific and efficient manner. The Type II CRISPR-Cas9 system enables gene editing by directed DNA cleavage followed by either non-homologous end joining (NHEJ) or homology-directed repair (HDR). Here, we described the use of the Type II CRISPR-Cas9 system in detail from designing the guides to 作者: Debility 時間: 2025-3-26 01:58 作者: Commodious 時間: 2025-3-26 04:51 作者: 反感 時間: 2025-3-26 09:08 作者: 使服水土 時間: 2025-3-26 16:04 作者: Focus-Words 時間: 2025-3-26 20:28
Analysis of Common Abnormalities Seen in Chronic Lymphocytic Leukemia Using Fluorescence In Situ Hyhas been employed widely in cytogenetics laboratories worldwide. This chapter describes techniques and trouble-shooting to maximize the efficiency of microscope slide preparation for FISH analysis in CLL.作者: 平躺 時間: 2025-3-27 00:30 作者: Arb853 時間: 2025-3-27 04:04
Gene Disruption Using CRISPR-Cas9 Technology,n a specific and efficient manner. The Type II CRISPR-Cas9 system enables gene editing by directed DNA cleavage followed by either non-homologous end joining (NHEJ) or homology-directed repair (HDR). Here, we described the use of the Type II CRISPR-Cas9 system in detail from designing the guides to analyzing the desired gene disruption events.作者: 不可知論 時間: 2025-3-27 08:44
Rare Variant Quantitation Using Droplet Digital PCR,an enumerate both mutant and wild-type alleles enabling highly sensitive detection and quantitation of the variant allele frequency of mutated genes. In this protocol, we describe a method for using ddPCR to detect mutations in genomic DNA with a sensitivity of up to 1 in 50,000 DNA copies.作者: Camouflage 時間: 2025-3-27 10:22
Methods for Measuring ctDNA in Lymphomas,tary source of tumor DNA to tissue biopsy for genotyping. Applications of cfDNA analysis in lymphomas include: (1) identification of tumor mutations in a biopsy-free manner; (2) tracking tumor clonal evolution and identification of mutations causing resistance to treatment; and (3) monitoring of residual disease after therapy.作者: ensemble 時間: 2025-3-27 15:13
Sami N. MalekInclude cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implemenation advice from the experts作者: CORD 時間: 2025-3-27 20:58
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/226392.jpg作者: sundowning 時間: 2025-3-28 00:16 作者: 表被動 時間: 2025-3-28 02:24 作者: 祖先 時間: 2025-3-28 10:10 作者: 畏縮 時間: 2025-3-28 11:54 作者: 躲債 時間: 2025-3-28 15:05 作者: leniency 時間: 2025-3-28 18:56
Ideologies, Worldviews, and Personalities, or lethal phenotype under various conditions in organisms and tissues. Here, we describe a complete protocol for scalable CRISPR/Cas9-based dropout screening for essential genes from focused genomic regions to whole genomes.作者: 喃喃訴苦 時間: 2025-3-29 01:47 作者: 空氣傳播 時間: 2025-3-29 04:07 作者: alliance 時間: 2025-3-29 09:44 作者: 后天習得 時間: 2025-3-29 13:08
CRISPR/Cas9-Based Gene Dropout Screens, or lethal phenotype under various conditions in organisms and tissues. Here, we describe a complete protocol for scalable CRISPR/Cas9-based dropout screening for essential genes from focused genomic regions to whole genomes.作者: BUCK 時間: 2025-3-29 17:40 作者: 折磨 時間: 2025-3-29 21:58 作者: neutralize 時間: 2025-3-30 03:14
Book 2019wn pitfalls..?Authoritative and cutting-edge, .Chronic Lymphocytic?.Leukemia.: Methods and Protocols .aims to accelerate research on chronic lymphocytic leukemia?and further improvements in patient outcomes.?.作者: 我不重要 時間: 2025-3-30 05:04 作者: 詞匯表 時間: 2025-3-30 10:50
,Reflection on Teachers’ Roles,therapy and the novel drugs targeting the BCR pathway, here we describe the flow cytometric approaches followed by us to quantify the CD49d expression levels and the VLA-4 activation status in CLL cells.作者: 悄悄移動 時間: 2025-3-30 15:54
Adolescents and Cultures of Reflectionh high and very low level MRD, respectively. This chapter has a particular focus on samples acquired shortly after anti-CD20 treatment. The standardization developed by the EuroFlow consortium is additionally described as technical basis for reproducible and standardized flow cytometric MRD assessments.作者: 彈藥 時間: 2025-3-30 16:47 作者: 承認 時間: 2025-3-30 22:37
Minimal Residual Disease Quantification in Chronic Lymphocytic Leukemia: Clinical Significance and h high and very low level MRD, respectively. This chapter has a particular focus on samples acquired shortly after anti-CD20 treatment. The standardization developed by the EuroFlow consortium is additionally described as technical basis for reproducible and standardized flow cytometric MRD assessments.作者: Eulogy 時間: 2025-3-31 03:39 作者: chance 時間: 2025-3-31 06:49
Visuals in the Special Needs Classroom determined. While adherent cell lines are easy to use for this assay, suspension cultures or primary cells are difficult to use. In the following sections, we describe the methodology for this assay for CLL cells in suspension cultures and CLL-stroma cocultures.作者: 排斥 時間: 2025-3-31 09:44
https://doi.org/10.1007/978-94-011-4972-3):2313–2324, 2017). It is important to note that immunoblotting should always be combined with other quantitative methods such as flow cytometry to confirm activation of these signaling pathways (Aguilar-Hernandez et al. Blood 127(24):3015–3025, 2016).作者: APRON 時間: 2025-3-31 17:07
Stage 1: Enhancing Self-Awarenessve allowed development of isogenic models and detailed analysis of changes to the transcriptome with relative ease. In this manuscript, we focus on two relevant methods to study splicing factor mutations in CLL: development of isogenic scalable cell lines and informatics analysis of RNA-Seq datasets.作者: 漸變 時間: 2025-3-31 19:48 作者: 羊欄 時間: 2025-4-1 01:46
Reflective Practice in Teachingrate methods to measure their activities. Here, we describe a series of reliable assays capable of measuring important antibody-mediated effector functions: antibody-dependent cellular phagocytosis (ADCP), antibody-dependent cellular cytotoxicity (ADCC), and complement-dependent cytotoxicity (CDC) that measure these immune activities.作者: 統(tǒng)治人類 時間: 2025-4-1 05:35
Ex-Vivo Signal Transduction Studies in Chronic Lymphocytic Leukemia,):2313–2324, 2017). It is important to note that immunoblotting should always be combined with other quantitative methods such as flow cytometry to confirm activation of these signaling pathways (Aguilar-Hernandez et al. Blood 127(24):3015–3025, 2016).作者: 防水 時間: 2025-4-1 07:26
The Development and Use of Scalable Systems for Studying Aberrant Splicing in SF3B1-Mutant CLL,ve allowed development of isogenic models and detailed analysis of changes to the transcriptome with relative ease. In this manuscript, we focus on two relevant methods to study splicing factor mutations in CLL: development of isogenic scalable cell lines and informatics analysis of RNA-Seq datasets.作者: 使尷尬 時間: 2025-4-1 11:24 作者: 蒸發(fā) 時間: 2025-4-1 16:07 作者: 媒介 時間: 2025-4-1 18:43
Ex-Vivo Signal Transduction Studies in Chronic Lymphocytic Leukemia,th protein and chemical methods is crucial if we are to investigate and correlate biological changes with therapeutic responses in patients. Herein, we describe the use of western blotting also referred to as immunoblotting as a method that can semiquantitatively evaluate changes in protein expressi作者: 真實的人 時間: 2025-4-1 23:08
Ex Vivo Pharmacological Profiling in Chronic Lymphocytic Leukemia Cells,e the first agent is administered to patients and second drug is tested in these patients’ cells in vitro. These assays have been effective in identifying novel agents that work additively or synergistically. In this chapter, we provide a step-by-step protocol for ex vivo drug testing that can be us