標(biāo)題: Titlebook: Chromosome and Genomic Engineering in Plants; Methods and Protocol Minoru Murata Book 2016 Springer Science+Business Media New York 2016 pl [打印本頁] 作者: 自治 時間: 2025-3-21 19:13
書目名稱Chromosome and Genomic Engineering in Plants影響因子(影響力)
書目名稱Chromosome and Genomic Engineering in Plants影響因子(影響力)學(xué)科排名
書目名稱Chromosome and Genomic Engineering in Plants網(wǎng)絡(luò)公開度
書目名稱Chromosome and Genomic Engineering in Plants網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Chromosome and Genomic Engineering in Plants被引頻次
書目名稱Chromosome and Genomic Engineering in Plants被引頻次學(xué)科排名
書目名稱Chromosome and Genomic Engineering in Plants年度引用
書目名稱Chromosome and Genomic Engineering in Plants年度引用學(xué)科排名
書目名稱Chromosome and Genomic Engineering in Plants讀者反饋
書目名稱Chromosome and Genomic Engineering in Plants讀者反饋學(xué)科排名
作者: parallelism 時間: 2025-3-21 22:38
Method for Biolistic Site-Specific Integration in Plants Catalyzed by Bxb1 Integrase,to existing cultivars. Previously, we described a recombinase-directed gene stacking method in tobacco (Hou et al., Mol Plant 7:1756–1765, 2014). Being able to stack DNA to a previous location ensures that the number of genetic loci does not increase with each new round of transgene addition. Wherea作者: 懸掛 時間: 2025-3-22 03:30
Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase-Mediated Gene Stacking,ly stack DNA molecules in vitro and that the in vitro derived gene stack can be incorporated into an . transformation vector by in vitro recombination. After transfer to the chromosome by Agroinfection, the transgenic locus harbors a new target site that can be used for the subsequent in vivo stacki作者: 先鋒派 時間: 2025-3-22 07:13 作者: 揮舞 時間: 2025-3-22 12:11 作者: Incommensurate 時間: 2025-3-22 16:33 作者: Incommensurate 時間: 2025-3-22 17:08 作者: notion 時間: 2025-3-22 22:44
CRISPR/Cas-Mediated Site-Specific Mutagenesis in , Using Cas9 Nucleases and Paired Nickases,ng the target sequence and its applicability to organisms throughout all kingdoms of life. The need for an easy-to-use and reliable nuclease is especially high in plant research, as precise genome modifications are almost impossible to achieve by .-mediated transformation and the regeneration of pla作者: insecticide 時間: 2025-3-23 02:58 作者: 毗鄰 時間: 2025-3-23 07:49
Seamless Genome Editing in Rice via Gene Targeting and Precise Marker Elimination,spectively, allows enrichment of cells harboring target genes modified via gene targeting (GT). We have developed a successful GT system employing positive–negative selection and subsequent precise marker excision via the . transposon derived from the cabbage looper moth to introduce desired modific作者: 不能妥協(xié) 時間: 2025-3-23 12:19 作者: 全神貫注于 時間: 2025-3-23 17:18
Chromosomal Allocation of DNA Sequences in Wheat Using Flow-Sorted Chromosomes, repetitive DNA sequences. Despite the recent progress in the analysis of plant genome organization and chromosome structure, there is a need for easy methods to assign DNA sequences to individual chromosomes. Here, we describe an easy way to allocate genes or DNA sequences to chromosomes in wheat u作者: META 時間: 2025-3-23 21:25
Image Analysis of DNA Fiber and Nucleus in Plants,sable tools where morphology, density, and color play important roles in the biological systems. Visualization and image analysis methods are useful techniques in the analyses of the detailed structure and function of extended DNA fibers (EDFs) and interphase nuclei. The EDF is the highest in the sp作者: 十字架 時間: 2025-3-24 01:56
Detection of Transgenes on DNA Fibers,resolution of FISH on chromosomes is limited by condensed structure of chromatin, and it is difficult to differentiate two target sites close to each other. To overcome this issue, the objects was changed to stretched DNA fibers, and this fiber FISH technique has now been used for revealing genome s作者: Grating 時間: 2025-3-24 05:59
Three-Dimensional, Live-Cell Imaging of Chromatin Dynamics in Plant Nuclei Using Chromatin Tagging in the nuclei of plants. Here, we introduce a three-dimensional, live-cell imaging method that can monitor chromatin dynamics in nuclei via a chromatin tagging system that can visualize specific genomic loci in living plant cells. The chromatin tagging system is based on a bacterial operator/repress作者: 擔(dān)心 時間: 2025-3-24 06:37
Chromatin Immunoprecipitation for Detecting Epigenetic Marks on Plant Nucleosomes,uding modified histones and specific histone variants (e.g., centromere-specific histone H3, CENH3) in this decade. Here, I describe a sensitive and low-background ChIP protocol for a wide range of plant species.作者: 四牛在彎曲 時間: 2025-3-24 13:30
Mapping of T-DNA and ,/, by TAIL-PCR to Analyze Chromosomal Rearrangements, transposons . (.). (.) have been systematically used to manipulate plant chromosomes. For both of these applications, the recovery of genomic DNA sequences flanking the insertions is required to estimate the sizes and/or scales of the reconstituted chromosomes. In this chapter, we describe the prot作者: locus-ceruleus 時間: 2025-3-24 17:45 作者: nauseate 時間: 2025-3-24 22:25 作者: 壓碎 時間: 2025-3-24 23:42 作者: 分開如此和諧 時間: 2025-3-25 07:08
https://doi.org/10.1007/978-1-4020-6979-6on mechanisms leading to such genomic rearrangements were analyzed. The rearrangements were induced by sister chromatid transposition (SCT), homologous recombination (HR), and single chromatid transposition (SLCT). Among them, 22 events (65?%) were found to be transmitted to the next generation. The作者: PALL 時間: 2025-3-25 08:55
Mary Ann Cooper,Ronald L. Holle a series of dots. The size of DNA in the dot is estimated to be approximately 1 kb, it corresponding to the resolution of fiber FISH. This makes it possible to analyze structures of transgenes on DNA fibers in detail.作者: Dawdle 時間: 2025-3-25 15:27 作者: 煩憂 時間: 2025-3-25 17:44
Minoru MurataIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 宮殿般 時間: 2025-3-25 20:06 作者: Lethargic 時間: 2025-3-26 02:11
Appendix: Decomposition with Differencingminichromosomes relies on telomere-mediated chromosomal truncation, which involves introducing transgenes and telomere sequences concurrently to the cell to truncate an endogenous chromosomal target. Two methods can be utilized; either the telomere sequences can be incorporated into a binary vector 作者: 強化 時間: 2025-3-26 06:02 作者: 分發(fā) 時間: 2025-3-26 09:45
Appendix: Decomposition with Differencingly stack DNA molecules in vitro and that the in vitro derived gene stack can be incorporated into an . transformation vector by in vitro recombination. After transfer to the chromosome by Agroinfection, the transgenic locus harbors a new target site that can be used for the subsequent in vivo stacki作者: 流浪 時間: 2025-3-26 16:02 作者: vitrectomy 時間: 2025-3-26 18:31
https://doi.org/10.1007/978-1-4020-6979-6letional mutagenesis, as well as for the generation of artificial ring chromosomes in model plants such as Arabidopsis and tobacco. However, it takes a long time to complete this process, even in Arabidopsis. To overcome this issue, a new binary vector, pDLHC, has been developed to induce chromosoma作者: 印第安人 時間: 2025-3-26 23:17 作者: KIN 時間: 2025-3-27 03:30 作者: 啞劇 時間: 2025-3-27 06:01 作者: exceed 時間: 2025-3-27 10:04 作者: Aboveboard 時間: 2025-3-27 16:47
Guillaume Cohen,Maxime Ladaiquespectively, allows enrichment of cells harboring target genes modified via gene targeting (GT). We have developed a successful GT system employing positive–negative selection and subsequent precise marker excision via the . transposon derived from the cabbage looper moth to introduce desired modific作者: 慷慨援助 時間: 2025-3-27 20:54
Guillaume Cohen,Maxime Ladaiquengineering of PPR motifs as new RNA/DNA manipulation tools in living cells, including for genome editing. However, the biochemical characteristics of PPR proteins remain unknown, mostly due to the instability and/or unfolding propensities of PPR proteins in heterologous expression systems such as ba作者: 尊敬 時間: 2025-3-28 02:00
The European Union Social Tools in Question repetitive DNA sequences. Despite the recent progress in the analysis of plant genome organization and chromosome structure, there is a need for easy methods to assign DNA sequences to individual chromosomes. Here, we describe an easy way to allocate genes or DNA sequences to chromosomes in wheat u作者: 無價值 時間: 2025-3-28 05:00 作者: 向外 時間: 2025-3-28 07:13
Mary Ann Cooper,Ronald L. Holleresolution of FISH on chromosomes is limited by condensed structure of chromatin, and it is difficult to differentiate two target sites close to each other. To overcome this issue, the objects was changed to stretched DNA fibers, and this fiber FISH technique has now been used for revealing genome s作者: Maximizer 時間: 2025-3-28 11:43 作者: 向下五度才偏 時間: 2025-3-28 15:00
Mary Ann Cooper,Ronald L. Holleuding modified histones and specific histone variants (e.g., centromere-specific histone H3, CENH3) in this decade. Here, I describe a sensitive and low-background ChIP protocol for a wide range of plant species.作者: metropolitan 時間: 2025-3-28 20:32 作者: CREST 時間: 2025-3-28 23:08 作者: 雄偉 時間: 2025-3-29 04:58 作者: 遺產(chǎn) 時間: 2025-3-29 09:01
Chromatin Immunoprecipitation for Detecting Epigenetic Marks on Plant Nucleosomes,uding modified histones and specific histone variants (e.g., centromere-specific histone H3, CENH3) in this decade. Here, I describe a sensitive and low-background ChIP protocol for a wide range of plant species.作者: CALL 時間: 2025-3-29 11:29
https://doi.org/10.1007/978-1-4939-4931-1plant genetics & genomics; DNA insertion; plant chromosome vector; plant artificial chromosomes; Cre/Lox作者: appall 時間: 2025-3-29 16:46 作者: CRUMB 時間: 2025-3-29 20:12
The European Union Social Tools in Question occurs stochastically in transgenic cells and SSN-mediated targeted mutagenesis often leads to no selectable phenotype, identification of highly mutated cell lines is a critical step in obtaining regenerated plants with desired mutations.作者: Gorilla 時間: 2025-3-30 00:55 作者: 合法 時間: 2025-3-30 06:11
Time of Day and Time of Year of Lightningescent signals within a nucleus and monitor the distance between homologous loci. These live-cell imaging methods will provide new insights into genome organization, development processes, and subnuclear responses to environmental stimuli in plants.作者: 確定方向 時間: 2025-3-30 11:33
Targeted Mutagenesis in Rice Using TALENs and the CRISPR/Cas9 System, occurs stochastically in transgenic cells and SSN-mediated targeted mutagenesis often leads to no selectable phenotype, identification of highly mutated cell lines is a critical step in obtaining regenerated plants with desired mutations.作者: concert 時間: 2025-3-30 14:02
Development of Genome Engineering Tools from Plant-Specific PPR Proteins Using Animal Cultured Cell ratio (>80?%); and rapid, high-throughput data production. In this chapter, we introduce an example of such reporter systems: a PPR-based sequence-specific translational activation system. The cell-based reporter system can be applied to characterize plant genes of interested and to PPR engineering.作者: 共同時代 時間: 2025-3-30 20:21
Three-Dimensional, Live-Cell Imaging of Chromatin Dynamics in Plant Nuclei Using Chromatin Tagging escent signals within a nucleus and monitor the distance between homologous loci. These live-cell imaging methods will provide new insights into genome organization, development processes, and subnuclear responses to environmental stimuli in plants.作者: SEED 時間: 2025-3-30 20:59 作者: DAMP 時間: 2025-3-31 04:07 作者: FLAX 時間: 2025-3-31 08:14
https://doi.org/10.1007/978-1-4020-6979-6Nihon bare” expressing . TPase. pDLHC has potential for the efficient generation of various types of chromosomal rearrangements including deletions, inversions, translocations and artificial ring chromosomes in plants, and the detailed protocol for rice is described here.作者: Insulin 時間: 2025-3-31 12:52 作者: Protein 時間: 2025-3-31 17:22 作者: RAG 時間: 2025-3-31 18:01
One-Step Generation of Chromosomal Rearrangements in Rice,Nihon bare” expressing . TPase. pDLHC has potential for the efficient generation of various types of chromosomal rearrangements including deletions, inversions, translocations and artificial ring chromosomes in plants, and the detailed protocol for rice is described here.
