作者: 搜集 時(shí)間: 2025-3-21 21:22
https://doi.org/10.1007/978-3-662-25094-5detailed characterization of those chromosomal structures signaling positive for the PRINS reaction. The combined efforts of FITC signals, DAPI-, PI-, and C-bands are most precisely analyzed through the use of a microscope mounted with a cooled CCD camera and an auto-wheel fluorescence filter set regulated by a computer.作者: 真實(shí)的人 時(shí)間: 2025-3-22 03:12
The Construction Logic of a New Class Schemaed FISH procedure for the detection of small and single copy transgenes within the rice genome is described and the application of FISH to evaluate chromosomal location and the local chromatin structure of transgenes as parameters that could affect their expression is discussed.作者: 為敵 時(shí)間: 2025-3-22 07:10 作者: FACT 時(shí)間: 2025-3-22 09:14 作者: PALL 時(shí)間: 2025-3-22 16:58
Relationship of telomere sequence and constitutive heterochromatin in the human and apes as detectedetailed characterization of those chromosomal structures signaling positive for the PRINS reaction. The combined efforts of FITC signals, DAPI-, PI-, and C-bands are most precisely analyzed through the use of a microscope mounted with a cooled CCD camera and an auto-wheel fluorescence filter set regulated by a computer.作者: PALL 時(shí)間: 2025-3-22 17:51 作者: 大洪水 時(shí)間: 2025-3-23 00:01 作者: braggadocio 時(shí)間: 2025-3-23 04:48
Microdissection and chromosome painting of plant B chromosomes,equences which are responsible for the painting of mammalian chromosomes. The feasibility of ‘chromosome painting’ of standard chromosomes in plant species with relatively small or large genomes is discussed.作者: 抵押貸款 時(shí)間: 2025-3-23 06:12 作者: 爭議的蘋果 時(shí)間: 2025-3-23 09:56 作者: lactic 時(shí)間: 2025-3-23 16:51 作者: GULP 時(shí)間: 2025-3-23 20:27 作者: organism 時(shí)間: 2025-3-24 01:10 作者: 使殘廢 時(shí)間: 2025-3-24 04:36 作者: Judicious 時(shí)間: 2025-3-24 10:02 作者: 上流社會(huì) 時(shí)間: 2025-3-24 12:29
https://doi.org/10.1057/9780230379220purified, then used to localize both proteins on spreads of unfixed metaphasic chromosomes. We show here, by conventional and confocal microscopy, that both proteins are localized at centromeres and pericentromeres, and that HP1γ is also present on euchromatic sites of chromosome arms.作者: 600 時(shí)間: 2025-3-24 15:12
,Chromosome painting — principles, strategies and scope,ion of probes, hybridization and visualization. The impact of this method lies in identification of genes for desired characters in the chromosomes, including those of genetic disorders, in cancer research, in transgenesis and in studies on biodiversity and evolution.作者: 狂亂 時(shí)間: 2025-3-24 20:03 作者: Intersect 時(shí)間: 2025-3-24 23:50
Mark B. Cannon,S. James Remingtonisochores. . hybridization of DNA from different isochore families on metaphase chromosomes allow to distinguish different sets of Giemsa and Reverse bands. In addition, it also provides information on the chromosomal distribution of genes.作者: 膽汁 時(shí)間: 2025-3-25 04:12
https://doi.org/10.1057/9780230504592s also given new insights into somaclonal variation, the origin of B chromosomes, control of chromosome pairing and other aspects of chromosome evolution. This review highlights some of these areas and provides source material for the reader who seeks more information on a specific field.作者: 反叛者 時(shí)間: 2025-3-25 10:58 作者: 聾子 時(shí)間: 2025-3-25 15:28 作者: debris 時(shí)間: 2025-3-25 18:23
Book 2001aid of specific probes in molecular hybridization. Its resolution ranges from single copy to entire genome sequences. It is now applied in plant, animal, and human systems, in gene mapping, identification of genetic disorders, evolutionary studies, and gene transfer experiments. This treatise is the作者: 開始從未 時(shí)間: 2025-3-25 22:53 作者: Pelvic-Floor 時(shí)間: 2025-3-26 00:44
Chromosome painting in farm, pet and wild animal species,pared to others. The comparisons provide insight into the likely constitution of a vertebrate/mammalian ancestral karyotype and help understand some of the intricacies about karyotype evolution. Importance of comparative painting in setting the stage for rapid development of gene maps in a number of作者: CROW 時(shí)間: 2025-3-26 05:19 作者: PAEAN 時(shí)間: 2025-3-26 09:43
Localisation of DNA sequences on plant chromosomes using PRINS and C-PRINS,ent centrifugation and/or flow sorting. The methods result in clear signals with negligible non-specific labelling. Further work is needed to improve the sensitivity to allow for reliable detection of single-copy DNA sequences.作者: 討人喜歡 時(shí)間: 2025-3-26 14:16
Fluorescent , hybridization in plant polytene chromosomes,lant polytenics, seem to be the most important hindrance for a precise chromosome mapping. On the other hand, the interphase polytene nucleus is a valuable system for localizing FISH signals since they conserve a spatial organization similar to that of mitotic interphase and produce much amplified s作者: Presbyopia 時(shí)間: 2025-3-26 16:59
High resolution FISH to delineate contiguous and small DNA sequences,ridization incubation and post hybridization stringency washing facilitates spatial resolution of contiguous repetitive rye probes pSc 200 and pSc 250, and physical localisation of small RFLP probe xpr 115 of wheat on barley chromosomes.作者: Inculcate 時(shí)間: 2025-3-27 00:47
Comparative karyotype using bidirectional chromosome painting: how and why?,rove knowledge on species evolution but also to better understand carcinogenesis process, as illustrated by our data concerning the cytogenetic characterization of radon-induced rat lung tumors. Detailed methods for obtaining painting probes by PARM-PCR from sorted mouse and rat chromosomes and for 作者: 致命 時(shí)間: 2025-3-27 01:47
https://doi.org/10.1007/978-1-59745-129-1for chromosome preparation from cultured tumour cells are complemented with a prolonged trypsin treatment to obtain complete disaggregation of cells, and exposure of the metaphase spreads to detergent and saline at high temperature, followed by pepsin digestion to remove extracellular matrix and cyt作者: anchor 時(shí)間: 2025-3-27 07:47 作者: 游行 時(shí)間: 2025-3-27 12:20
The Fierro Years: An Exercise in Melancholy,osome-specific DNA probes have so far not revealed convincing results in plants. The reason for this failure and the use of possible alternative approaches are discussed. At least for small plant genomes, painting by large insert single sequence clones provides a promising alternative tool to solve 作者: Eclampsia 時(shí)間: 2025-3-27 17:03
,Memín Pinguin: Líos Gordos con los Gringos,ent centrifugation and/or flow sorting. The methods result in clear signals with negligible non-specific labelling. Further work is needed to improve the sensitivity to allow for reliable detection of single-copy DNA sequences.作者: inquisitive 時(shí)間: 2025-3-27 20:05 作者: 大廳 時(shí)間: 2025-3-27 22:39
https://doi.org/10.1057/9780230511361ridization incubation and post hybridization stringency washing facilitates spatial resolution of contiguous repetitive rye probes pSc 200 and pSc 250, and physical localisation of small RFLP probe xpr 115 of wheat on barley chromosomes.作者: Incommensurate 時(shí)間: 2025-3-28 04:51
https://doi.org/10.1057/9780230511361rove knowledge on species evolution but also to better understand carcinogenesis process, as illustrated by our data concerning the cytogenetic characterization of radon-induced rat lung tumors. Detailed methods for obtaining painting probes by PARM-PCR from sorted mouse and rat chromosomes and for 作者: antipsychotic 時(shí)間: 2025-3-28 09:40 作者: 偽造者 時(shí)間: 2025-3-28 10:26 作者: 反饋 時(shí)間: 2025-3-28 14:37 作者: 纖細(xì) 時(shí)間: 2025-3-28 21:32 作者: 欲望小妹 時(shí)間: 2025-3-28 23:48 作者: Concomitant 時(shí)間: 2025-3-29 04:05 作者: synovium 時(shí)間: 2025-3-29 10:46
Chromosome painting in plants,hybridisation (GISH) with total genomic DNA to distinguish alien chromosomes on the basis of divergent dispersed repeats, ii) ‘Chromosomal . suppression’ (CISS) hybridisation with chromosome-derived DNA probes and blocking of interchromosomally dispersed repeats by total genomic or C..-1 DNA in exce作者: saturated-fat 時(shí)間: 2025-3-29 14:48
Localisation of DNA sequences on plant chromosomes using PRINS and C-PRINS,d is suitable for most applications it is time-consuming and requires labelled probes. Recently, primed . labelling (PRINS) has been developed as an alternative to FISH. PRINS is based on annealing of unlabelled oligonucleotide primer(s) to chromosome DNA and its elongation by DNA polymerase in the 作者: obsession 時(shí)間: 2025-3-29 15:59
GISH technology in plant genome research,eloped in recent years. It has enabled plant chromosome and genome research to progress at phenomenal rate and its potential is far from exhausted. Genomic . hybridization has applications in characterization of genomes and chromosomes in hybrid polyploids, hybrid plants, partial allopolyploids, pol作者: Ballerina 時(shí)間: 2025-3-29 21:55 作者: CLAIM 時(shí)間: 2025-3-30 02:25 作者: 乳汁 時(shí)間: 2025-3-30 05:02
Chromosome painting as a tool for rice genetics and breeding,e applied even in the typically small chromosomes of rice. This manuscript describes in detail, highly reproducible, complete protocols for chromosome painting and GISH in rice chromosomes. Examples of useful applications of these methods are also presented.作者: Inflamed 時(shí)間: 2025-3-30 11:03
Fluorescent , hybridization in plant polytene chromosomes,n several aspects, mainly because their chromatids on each chromosome are not tightly paired, nor are they so highly endoreplicated as those of Diptera. . hybridization with isotopic and non-isotopic probes has been successfully used in plant polytene chromosomes, mainly in . and ., where they have 作者: 猛擊 時(shí)間: 2025-3-30 16:03
Molecular cytogenetics of introgressive hybridization in plants,ession is important in the evolution of flowering plants. It is also important in plant breeding where a desirable trait can be transferred from wild to crop species. One of the most recent advances in molecular techniques for studying hybridization and introgression is . hybridization of genomic pr作者: 羊欄 時(shí)間: 2025-3-30 17:14
High resolution FISH to delineate contiguous and small DNA sequences,or weak/contiguous hybridization sites. Hybridization with a ‘350 family’ rye repetitive DNA probe pSc 200 characteristically differentiated the rye chromosome 5 from the rest of the complement on account of two small terminal homologous sites in the long arm, resolution of which is substantially im作者: 同謀 時(shí)間: 2025-3-30 22:59 作者: 無聊的人 時(shí)間: 2025-3-31 03:32 作者: 鐵砧 時(shí)間: 2025-3-31 06:46 作者: 星球的光亮度 時(shí)間: 2025-3-31 12:20 作者: 無底 時(shí)間: 2025-3-31 16:35 作者: 無能性 時(shí)間: 2025-3-31 20:08
Chromosome painting as a tool for rice genetics and breeding,e applied even in the typically small chromosomes of rice. This manuscript describes in detail, highly reproducible, complete protocols for chromosome painting and GISH in rice chromosomes. Examples of useful applications of these methods are also presented.作者: Benzodiazepines 時(shí)間: 2025-3-31 22:20
https://doi.org/10.1007/978-94-010-0330-8Chromosome; DNA; Telomere; evolution; gene transfer; genetics; hybridization作者: 貧困 時(shí)間: 2025-4-1 03:49
978-94-010-3840-9Springer Science+Business Media Dordrecht 2001作者: 馬籠頭 時(shí)間: 2025-4-1 08:42
John T. Hancock,Matthew Whitemans principally based on molecular hybridization . with sequence specific probes on chromosomes. Different strategies have been adopted for the preparation of probes, hybridization and visualization. The impact of this method lies in identification of genes for desired characters in the chromosomes, i
