標題: Titlebook: Chromosome Architecture; Methods and Protocol Mark C. Leake Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under [打印本頁] 作者: 引起極大興趣 時間: 2025-3-21 17:06
書目名稱Chromosome Architecture影響因子(影響力)
作者: 咒語 時間: 2025-3-21 20:56
Single-Molecule Narrow-Field Microscopy of Protein-DNA Binding Dynamics in Glucose Signal Transductused in bacteria. Here, we describe how these methods can be extended to larger eukaryotic, yeast cells, which contain subcellular compartments. We describe how to obtain single-molecule microscopy data but also how to analyze these data to track and obtain the stoichiometry of molecular complexes d作者: 音樂學(xué)者 時間: 2025-3-22 01:23
Convolutional Neural Networks for Classifying Chromatin Morphology in Live-Cell Imaging,in machine learning have enabled researchers to automatically classify chromatin morphology in fluorescence microscopy images. In this protocol, we develop user-friendly tools to perform this task. We provide an open-source annotation tool, and a cloud-based computational framework to train and util作者: 性學(xué)院 時間: 2025-3-22 07:44
Fluorescence Recovery After Photobleaching (FRAP) to Study Dynamics of the Structural Maintenance oescently tagged MukBEF forms distinct spots (or “foci”) composed of molecular assemblies in the cell, where it is thought to carry out most of its chromosome-associated activities. Here, we outline the technique of fluorescence recovery after photobleaching (FRAP) as a method to study the properties作者: Cuisine 時間: 2025-3-22 11:27
Atomic Force Microscopy of DNA and DNA-Protein Interactions,ications, one of its key advantages is its ability to visualize the substructure of single molecules and molecular complexes in an aqueous environment. Here, we describe the application of AFM to determine the secondary and tertiary structure of surface-bound DNA, and its interactions with proteins.作者: 假設(shè) 時間: 2025-3-22 14:46 作者: 假設(shè) 時間: 2025-3-22 18:20 作者: Hiatus 時間: 2025-3-22 23:27
Atomistic Molecular Dynamics Simulations of DNA in Complex 3D Arrangements for Comparison with Loweng detail at a resolution where experimental techniques cannot arrive. Molecular dynamics (MD) simulations of mechanically distorted DNA caused by agents like supercoiling and protein binding are computationally challenging due to the large size of the associated systems and timescales. However, now作者: 顯示 時間: 2025-3-23 02:22 作者: 投射 時間: 2025-3-23 09:29
DNA-Protein Interactions Studied Directly Using iSCAT Imaging of GNP-Tagged Proteins,eatures are facile to include in a study. For single-molecule imaging this can be more difficult, because the constraints of the assay limit the variety of adducts that can be used. Surface-immobilized DNA provides an ideal compromise, and the use of interferometric scattering microscopy allows for 作者: BROTH 時間: 2025-3-23 12:35
Chromosome Copy Number Measurement Using Flow Cytometry Analysis,cells and by detecting cell-scattered light their number and relative size can be measured as they pass through the beam. Labeling of molecules, usually via a fluorescent reporter, allows the amount of these molecules per cell to be quantified. DNA content can be estimated using this approach and he作者: Gratulate 時間: 2025-3-23 17:05 作者: 追蹤 時間: 2025-3-23 20:16 作者: 鬼魂 時間: 2025-3-24 00:20 作者: 負擔 時間: 2025-3-24 02:31 作者: Adrenal-Glands 時間: 2025-3-24 07:09 作者: A保存的 時間: 2025-3-24 12:12 作者: 針葉 時間: 2025-3-24 18:09
Visualizing the Replisome, Chromosome Breaks, and Replication Restart in ,o ensure their correct replication and segregation into progeny cells. Here we discuss techniques that can be used with live bacterial cells to analyze DNA replisome dynamics, double-strand chromosome breaks, and restart of repaired replication forks.作者: RALES 時間: 2025-3-24 22:06
High-Throughput Imaging of ,o cytologically analyze every mutant and screen for new genes involved in cell shape determination, cell division, and chromosome segregation. Here we describe a high-throughput method to image arrayed . mutant libraries using wide-field fluorescence microscopy. We provide a detailed description of 作者: Agnosia 時間: 2025-3-25 01:30
https://doi.org/10.1007/978-981-15-7941-7ize a convolutional neural network to automatically classify chromatin morphology. Using cloud compute enables users without significant resources or computational experience to use a machine learning approach to analyze their own microscopy data.作者: Evocative 時間: 2025-3-25 06:26
1064-3745 expertsThis detailed new edition collects cutting-edge laboratory protocols, techniques, and applications in use by some of the leading international experts in the broad field of chromosome architecture. The book emphasizes the increasing physiological relevance of chromosome architecture investig作者: 會議 時間: 2025-3-25 07:30
https://doi.org/10.1007/978-981-15-7941-7omosome-associated activities. Here, we outline the technique of fluorescence recovery after photobleaching (FRAP) as a method to study the properties of YFP-tagged MukB in fluorescent foci. This method can provide important insight into the dynamics of MukB on DNA and be used to study its biochemical properties in vivo.作者: 冥界三河 時間: 2025-3-25 13:24
Educational Writings in Chicago Yearse describe a method allowing the direct visualization of bacterial conjugation in live cells, including the fluorescent labeling of the conjugative pilus and the monitoring of plasmid DNA transfer from donor to recipient cells.作者: archaeology 時間: 2025-3-25 16:25 作者: Pepsin 時間: 2025-3-26 00:01
Educational Writings in Columbia Yearsent three approaches which enable the mechanics to be probed under varying conditions. This includes fully adhered cells, initially adhered cells which lack an established cytoskeleton, and purified nuclei to study their isolated response.作者: Occlusion 時間: 2025-3-26 01:25
Young Dewey and Zeitgeist in Psychologyed method of protein copy number quantification directly in living cells. This enables quick and reliable estimations and comparison of the protein of interest abundance without implementing large-scale studies.作者: 同音 時間: 2025-3-26 08:21 作者: Morphine 時間: 2025-3-26 09:02
Fluorescence Recovery After Photobleaching (FRAP) to Study Dynamics of the Structural Maintenance oomosome-associated activities. Here, we outline the technique of fluorescence recovery after photobleaching (FRAP) as a method to study the properties of YFP-tagged MukB in fluorescent foci. This method can provide important insight into the dynamics of MukB on DNA and be used to study its biochemical properties in vivo.作者: Obituary 時間: 2025-3-26 15:55 作者: 做作 時間: 2025-3-26 19:22 作者: 憤怒歷史 時間: 2025-3-26 23:23
Measuring Nuclear Mechanics with Atomic Force Microscopy,ent three approaches which enable the mechanics to be probed under varying conditions. This includes fully adhered cells, initially adhered cells which lack an established cytoskeleton, and purified nuclei to study their isolated response.作者: bypass 時間: 2025-3-27 05:00
Copy Number Analysis of the Yeast Histone Deacetylase Complex Component Cti6 Directly in Living Celed method of protein copy number quantification directly in living cells. This enables quick and reliable estimations and comparison of the protein of interest abundance without implementing large-scale studies.作者: 夾死提手勢 時間: 2025-3-27 07:58
High-Throughput Imaging of , describe a high-throughput method to image arrayed . mutant libraries using wide-field fluorescence microscopy. We provide a detailed description of growing the arrayed strain collection, preparing slides containing agarose pedestals, setting up the microscopy procedure, acquiring images, and analyzing the images.作者: 調(diào)整 時間: 2025-3-27 10:27
Young Dewey and Zeitgeist in Psychologyas transcriptional regulation is fundamentally a single-molecule problem—a single repressor protein binding a single binding site in the genome can dramatically alter behavior at the whole cell and population levels.作者: Multiple 時間: 2025-3-27 15:52 作者: 售穴 時間: 2025-3-27 18:34
Single-Molecule Narrow-Field Microscopy of Protein-DNA Binding Dynamics in Glucose Signal Transductas transcriptional regulation is fundamentally a single-molecule problem—a single repressor protein binding a single binding site in the genome can dramatically alter behavior at the whole cell and population levels.作者: 健談 時間: 2025-3-28 01:58 作者: 態(tài)學(xué) 時間: 2025-3-28 05:16 作者: flimsy 時間: 2025-3-28 07:56 作者: Prognosis 時間: 2025-3-28 13:34
John Dewey and Progressive Education. Here we present detailed protocols for doing so: for performing molecular dynamics (MD) simulations of DNA adopting complex three-dimensional arrangements and for comparing the outcome of the calculations with single-molecule experimental data with a lower resolution than atomic.作者: floaters 時間: 2025-3-28 17:11 作者: 一個攪動不安 時間: 2025-3-28 20:34 作者: Mirage 時間: 2025-3-29 02:39
Atomistic Molecular Dynamics Simulations of DNA in Complex 3D Arrangements for Comparison with Lowe. Here we present detailed protocols for doing so: for performing molecular dynamics (MD) simulations of DNA adopting complex three-dimensional arrangements and for comparing the outcome of the calculations with single-molecule experimental data with a lower resolution than atomic.作者: Conflagration 時間: 2025-3-29 06:15
DNA-Protein Interactions Studied Directly Using iSCAT Imaging of GNP-Tagged Proteins,is technique is implemented and reveal software that can be used to deconvolute the images. Altogether, we hope to make this technique more accessible for studying specific DNA-protein interactions on tailored substrates.作者: Conflagration 時間: 2025-3-29 07:26 作者: 執(zhí)拗 時間: 2025-3-29 12:33 作者: 索賠 時間: 2025-3-29 19:20 作者: 暗諷 時間: 2025-3-29 20:37 作者: 善于騙人 時間: 2025-3-30 02:40
Young Dewey and Zeitgeist in Psychologyused in bacteria. Here, we describe how these methods can be extended to larger eukaryotic, yeast cells, which contain subcellular compartments. We describe how to obtain single-molecule microscopy data but also how to analyze these data to track and obtain the stoichiometry of molecular complexes d作者: stress-response 時間: 2025-3-30 05:14
https://doi.org/10.1007/978-981-15-7941-7in machine learning have enabled researchers to automatically classify chromatin morphology in fluorescence microscopy images. In this protocol, we develop user-friendly tools to perform this task. We provide an open-source annotation tool, and a cloud-based computational framework to train and util作者: 無可爭辯 時間: 2025-3-30 09:58 作者: Aqueous-Humor 時間: 2025-3-30 16:18 作者: 漂浮 時間: 2025-3-30 20:07 作者: 去世 時間: 2025-3-30 23:30 作者: BADGE 時間: 2025-3-31 03:09
John Dewey and Progressive Educationng detail at a resolution where experimental techniques cannot arrive. Molecular dynamics (MD) simulations of mechanically distorted DNA caused by agents like supercoiling and protein binding are computationally challenging due to the large size of the associated systems and timescales. However, now作者: reflection 時間: 2025-3-31 08:38 作者: Foregery 時間: 2025-3-31 11:21
https://doi.org/10.1007/978-981-15-7941-7eatures are facile to include in a study. For single-molecule imaging this can be more difficult, because the constraints of the assay limit the variety of adducts that can be used. Surface-immobilized DNA provides an ideal compromise, and the use of interferometric scattering microscopy allows for 作者: 漂浮 時間: 2025-3-31 14:23
https://doi.org/10.1007/978-981-15-7941-7cells and by detecting cell-scattered light their number and relative size can be measured as they pass through the beam. Labeling of molecules, usually via a fluorescent reporter, allows the amount of these molecules per cell to be quantified. DNA content can be estimated using this approach and he作者: 天氣 時間: 2025-3-31 21:31
Educational Writings in Columbia Yearsfined movements during the cell cycle, for example to reliably segregate daughter chromosomes. More recently, various labs have begun investigating also the short time dynamics (displacements during time intervals of 0.1?s–100?s), which should be related to the molecular structure. Probing these dyn作者: 無動于衷 時間: 2025-4-1 00:13
Educational Writings in Columbia Yearspplication to measure and analyze the mechanics, in particular the effective Young’s elastic modulus . of the mammalian nucleus in live cells. We present three approaches which enable the mechanics to be probed under varying conditions. This includes fully adhered cells, initially adhered cells whic作者: 藥物 時間: 2025-4-1 02:15
Young Dewey and Zeitgeist in Psychologypy numbers is essential for revealing and better understanding of cellular behavior and functions. Here we describe a single-molecule fluorescence-based method of protein copy number quantification directly in living cells. This enables quick and reliable estimations and comparison of the protein of作者: Acquired 時間: 2025-4-1 07:59
John Dewey and Progressive Educational processes on the molecular scale. These methods have also transformed the study of bacterial cell biology, which was previously obstructed by the limited spatial resolution of conventional microscopy. In the case of DNA-binding proteins, super-resolution microscopy can visualize the detailed spat作者: COLIC 時間: 2025-4-1 10:27 作者: Crohns-disease 時間: 2025-4-1 17:12
https://doi.org/10.1007/978-981-15-7941-7 to determine structure and help regulate gene expression. Much of this mechanical perturbation is due to molecular machines such as topoisomerases which must stretch and twist DNA as part of various functions including DNA repair and replication. While the broad-scale mechanical response of nucleic作者: OTTER 時間: 2025-4-1 20:33
https://doi.org/10.1007/978-3-319-93088-6o ensure their correct replication and segregation into progeny cells. Here we discuss techniques that can be used with live bacterial cells to analyze DNA replisome dynamics, double-strand chromosome breaks, and restart of repaired replication forks.作者: 陪審團每個人 時間: 2025-4-1 23:52 作者: SHOCK 時間: 2025-4-2 05:24
Mark C. LeakeIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
