標題: Titlebook: Chromosome Architecture; Methods and Protocol Mark C. Leake Book 2016 Springer Science+Business Media New York 2016 dynamic architecture.ch [打印本頁] 作者: 粗略 時間: 2025-3-21 19:52
書目名稱Chromosome Architecture影響因子(影響力)
書目名稱Chromosome Architecture影響因子(影響力)學科排名
書目名稱Chromosome Architecture網(wǎng)絡(luò)公開度
書目名稱Chromosome Architecture網(wǎng)絡(luò)公開度學科排名
書目名稱Chromosome Architecture被引頻次
書目名稱Chromosome Architecture被引頻次學科排名
書目名稱Chromosome Architecture年度引用
書目名稱Chromosome Architecture年度引用學科排名
書目名稱Chromosome Architecture讀者反饋
書目名稱Chromosome Architecture讀者反饋學科排名
作者: CAJ 時間: 2025-3-21 21:06
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/226341.jpg作者: Phagocytes 時間: 2025-3-22 01:39
Matthias Kipping,Ian Kirkpatrick in interdisciplinary research methods at the cutting-edge interface of the life and physical sciences. Importantly this has involved several state-of-the-art biophysical tools used in conjunction with molecular biology approaches which enable investigation of chromosome structure and function in li作者: aesthetic 時間: 2025-3-22 07:11 作者: Organization 時間: 2025-3-22 11:39 作者: Debrief 時間: 2025-3-22 13:09 作者: Debrief 時間: 2025-3-22 18:14
All About Sports: Notes from an Excursionications, one of its key advantages is its ability to visualize substructure of single molecules and molecular complexes in an aqueous environment. Here, we describe the application of AFM to determine superstructure and secondary structure of surface-bound DNA. The method is also readily applicable作者: Resistance 時間: 2025-3-22 22:10
Introduction: Rediscovering Apprenticeship,s question. More recently, the parallel development of epifluorescence microscopy and genetic tools has enabled the direct visualization of the intracellular positioning of DNA sequences in live cells and has consequently revolutionized our view of the architecture of the nucleoid .. In this chapter作者: aggressor 時間: 2025-3-23 05:21
Introduction: Rediscovering Apprenticeship,also used to probe topological changes in DNA as a result of protein binding and enzymatic activity. However, in the longitudinal configuration, the DNA molecule is extended perpendicular to the imaging plane. As a result, it is only possible to infer biological activity from the motion of the tethe作者: DEBT 時間: 2025-3-23 09:23
Pollyanna Realism and the Simple Theory,e molecular mechanisms underlying bacterial chromosome architecture and dynamics during the cell cycle. Here we discuss techniques that can be used with live cells to analyze chromosome structure and segregation in the gram-positive model organism ..作者: OGLE 時間: 2025-3-23 12:52
Thomas Reid,Ludwig Wittgensteinderstanding this organization requires the integration of many data types and experimental results. Here we describe the approach of integrating genome-wide protein–DNA binding data to determine chromatin states. To investigate spatial aspects of genome organization, we present a detailed descriptio作者: 開始從未 時間: 2025-3-23 15:41 作者: wreathe 時間: 2025-3-23 21:23 作者: persistence 時間: 2025-3-24 00:32
https://doi.org/10.1007/978-3-663-12339-2cells and by detecting cell-scattered light their number and relative size can be measured as they pass through the beam. Labeling of molecules, usually via a fluorescent reporter, allows the amount of these molecules per cell to be quantified. DNA content can be estimated using this approach and he作者: 沉默 時間: 2025-3-24 06:25
Studies in Computational Intelligencets during the cell cycle, for example, to reliably segregate daughter chromosomes. More recently, various labs have begun investigating the short-time dynamics (displacements during time intervals of 0.1–100 s), which one hopes to link to structure, in analogy to “microrheology” approaches applied s作者: 驚呼 時間: 2025-3-24 08:12 作者: 代理人 時間: 2025-3-24 12:47 作者: Feigned 時間: 2025-3-24 17:31 作者: Badger 時間: 2025-3-24 20:40 作者: 過時 時間: 2025-3-25 01:22
Jeremy Grech,Mark Bugeja,Dylan Seychellrepair are driven by the mechanisms by which a functional nuclear protein finds its substrate in the nucleus. Single-particle tracking (SPT) is a method to quantify fluorescent molecules dynamics from the tracks of the single molecules recorded by high-resolution microscopes. SPT offers direct obser作者: CAB 時間: 2025-3-25 06:09
Educational Writings in Chicago Yearstation, . undergoes the development process of spore formation, which is among the simplest examples of cellular differentiation. Many aspects of these processes have benefited from fluorescence microscopy. Here, we describe basic wide-field fluorescence microscopy techniques to visualize . during v作者: CERE 時間: 2025-3-25 11:21 作者: Priapism 時間: 2025-3-25 11:43 作者: 受傷 時間: 2025-3-25 17:26 作者: Fibroid 時間: 2025-3-26 00:01 作者: CRACK 時間: 2025-3-26 04:10
978-1-4939-8100-7Springer Science+Business Media New York 2016作者: Harpoon 時間: 2025-3-26 05:37
https://doi.org/10.1057/9780230592827as transcriptional regulation is fundamentally a single-molecule problem—a single repressor protein binding a single binding site in the genome can dramatically alter behavior at the whole cell and population level.作者: 波動 時間: 2025-3-26 11:42 作者: 下垂 時間: 2025-3-26 14:40 作者: 格子架 時間: 2025-3-26 18:24
Intra-Nuclear Single-Particle Tracking (I-SPT) to Reveal the Functional Architecture of Chromosomesthod to record the trajectories of weakly fluorescent molecules in the nucleus of living cells. I-SPT uses some specific detection and analysis tools to enable the computation of reliable statistics on nuclear particle movement.作者: Creatinine-Test 時間: 2025-3-26 21:56
1064-3745 ation advice from the experts.This volume details a valuable collection of protocols andreviews, such as emerging experimental and theoretical approaches. Theseapproaches have resulted in a substantial improvement in the understanding ofchromosome architecture.?.Chromosome Architecture:Methods and P作者: dura-mater 時間: 2025-3-27 01:42
https://doi.org/10.1007/978-3-476-02834-1y After Photobleaching (FRAP) as a method to study the properties of YFP-tagged MukB in fluorescent foci. This method can provide important insight into the dynamics of MukB on DNA and be used to study its biochemical properties in vivo.作者: 美學 時間: 2025-3-27 07:59 作者: 符合國情 時間: 2025-3-27 12:07
https://doi.org/10.1007/978-3-663-12339-2ly via a fluorescent reporter, allows the amount of these molecules per cell to be quantified. DNA content can be estimated using this approach and here we describe how flow cytometry can be used to assess the DNA content of . cells.作者: 氣候 時間: 2025-3-27 16:08 作者: Perineum 時間: 2025-3-27 19:37
Using Fluorescence Recovery After Photobleaching (FRAP) to Study Dynamics of the Structural Mainteny After Photobleaching (FRAP) as a method to study the properties of YFP-tagged MukB in fluorescent foci. This method can provide important insight into the dynamics of MukB on DNA and be used to study its biochemical properties in vivo.作者: 宿醉 時間: 2025-3-28 00:16
Imaging DNA Structure by Atomic Force Microscopy,re, we describe the application of AFM to determine superstructure and secondary structure of surface-bound DNA. The method is also readily applicable to probe DNA–DNA interactions and DNA–protein complexes.作者: 的事物 時間: 2025-3-28 03:09
, Chromosome Copy Number Measurement Using Flow Cytometry Analysis,ly via a fluorescent reporter, allows the amount of these molecules per cell to be quantified. DNA content can be estimated using this approach and here we describe how flow cytometry can be used to assess the DNA content of . cells.作者: Eeg332 時間: 2025-3-28 08:44
Visualizing , During Vegetative Growth and Spore Formation,e processes have benefited from fluorescence microscopy. Here, we describe basic wide-field fluorescence microscopy techniques to visualize . during vegetative growth, and the developmental process of sporulation.作者: 魔鬼在游行 時間: 2025-3-28 11:07
Book 2016ulted in a substantial improvement in the understanding ofchromosome architecture.?.Chromosome Architecture:Methods and Protocols. guides readers through cutting-edge interdisciplinarymethods which allow for an understanding of architecture of chromosomes withexceptionally enhanced resolution, both 作者: 膽小懦夫 時間: 2025-3-28 15:22
Book 2016ctive topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls..Authoritativeand cutting-edge,.?.ChromosomeArchitecture: Methods and Protocols. .aims to ensure successful results in thefurther study of this vital field..作者: 補角 時間: 2025-3-28 22:48 作者: 昆蟲 時間: 2025-3-29 00:15 作者: Nebulous 時間: 2025-3-29 05:20 作者: chastise 時間: 2025-3-29 09:28
Studying the Dynamics of Chromatin-Binding Proteins in Mammalian Cells Using Single-Molecule Localie freely or do both. We can study the numbers of proteins that interact with chromatin and also determine their residence time on chromatin. We can determine whether these proteins form functional clusters within the nucleus as well as whether they form specific nuclear structures.作者: Outshine 時間: 2025-3-29 13:31 作者: resilience 時間: 2025-3-29 18:08
Investigating Bacterial Chromosome Architecture, I present a comprehensive methodology designed to characterize the architecture of the nucleoid DNA and the positioning of specific DNA sequences in live . cells. DNA localization systems, preparation of stable agarose-mounted microscopy slides, and basic image analysis tools are mentioned.作者: 煩擾 時間: 2025-3-29 20:56 作者: 后退 時間: 2025-3-30 03:34
In Vivo and In Situ Replication Labeling Methods for Super-resolution Structured Illumination Microls for highly efficient electroporation-based delivery or scratch loading of cell impermeable fluorescent nucleotides for live cell studies. Furthermore we describe the application of (2′S)-2′-deoxy-2′-fluoro-5-ethynyluridine (F-ara-EdU) for the in situ detection of segregated chromosome territories with minimized cytotoxic side effects.作者: 寄生蟲 時間: 2025-3-30 07:07
,DNA–Protein Interactions Studied Directly Using Single Molecule Fluorescence Imaging of Quantum Doteal-time fluorescence imaging of these interactions provides quantitative descriptions of search mechanism at the single molecule level. In our lab, we use this method to study the complex process of nucleotide excision DNA repair to determine mechanisms of damage detection, lesion removal, and DNA excision.作者: 使厭惡 時間: 2025-3-30 11:19 作者: 獨特性 時間: 2025-3-30 13:30
Redirections in the Study of Expert Labour of cargos, transport receptors and even structural components of the NPC to be determined with nanometre accuracy. In this protocol, we describe a method to study the position and/or motion of individual molecules transiting through the NPC with high spatial and temporal precision.作者: 使習慣于 時間: 2025-3-30 18:01 作者: 盲信者 時間: 2025-3-30 22:39 作者: 潰爛 時間: 2025-3-31 04:19
Why Colors Are Not Physical Properties,ls for highly efficient electroporation-based delivery or scratch loading of cell impermeable fluorescent nucleotides for live cell studies. Furthermore we describe the application of (2′S)-2′-deoxy-2′-fluoro-5-ethynyluridine (F-ara-EdU) for the in situ detection of segregated chromosome territories with minimized cytotoxic side effects.作者: 會議 時間: 2025-3-31 06:56
Theorie und Praxis neuer Staatlichkeiteal-time fluorescence imaging of these interactions provides quantitative descriptions of search mechanism at the single molecule level. In our lab, we use this method to study the complex process of nucleotide excision DNA repair to determine mechanisms of damage detection, lesion removal, and DNA excision.作者: synchronous 時間: 2025-3-31 12:10 作者: Esalate 時間: 2025-3-31 13:50 作者: COLIC 時間: 2025-3-31 21:02 作者: pancreas 時間: 2025-4-1 00:21
Rediscovering Heritage Through TechnologyGiven the concurrent improvements in the resolution of experimental techniques such as atomic force microscopy (AFM) and cryo-electron microscopy, the study of DNA minicircles will provide a more complete understanding of both the structure and the mechanics of supercoiled DNA.作者: 斗志 時間: 2025-4-1 04:37
Rediscovering Heritage Through TechnologyNA-binding proteins in . cells. The protocol covers the construction of cell strains and describes data acquisition and analysis procedures, such as super-resolution image reconstruction, mapping single-molecule tracks, computing diffusion coefficients to identify molecular subpopulations with diffe作者: 補助 時間: 2025-4-1 09:47 作者: heirloom 時間: 2025-4-1 12:03 作者: 陰謀小團體 時間: 2025-4-1 17:10
Transverse Magnetic Tweezers Allowing Coincident Epifluorescence Microscopy on Horizontally ExtendeA construct at molecular extensions approaching the contour length defined by B-DNA helical geometry, and the measured entropic elasticity agrees with the worm-like chain model (force?
作者: 逃避責任 時間: 2025-4-1 21:20 作者: ferment 時間: 2025-4-2 00:24
Super-Resolution Microscopy and Tracking of DNA-Binding Proteins in Bacterial Cells,NA-binding proteins in . cells. The protocol covers the construction of cell strains and describes data acquisition and analysis procedures, such as super-resolution image reconstruction, mapping single-molecule tracks, computing diffusion coefficients to identify molecular subpopulations with diffe
