標(biāo)題: Titlebook: Chromosomal Mutagenesis; Shondra M. Pruett-Miller Book 2015Latest edition Springer Science+Business Media New York 2015 Genome editing too [打印本頁] 作者: 詞源法 時(shí)間: 2025-3-21 16:31
書目名稱Chromosomal Mutagenesis影響因子(影響力)
作者: 挑剔為人 時(shí)間: 2025-3-22 00:10 作者: 卷發(fā) 時(shí)間: 2025-3-22 03:49
Using Phage Integrases in a Site-Specific Dual Integrase Cassette Exchange Strategy,C31 integrase performs efficient recombination between its . site and either its own placed . site or a partially mismatched genomic pseudo . site. Bxb1 integrase, another large serine recombinase, has a similar level of recombinational activity, but recognizes only its own . and . sites. Previously作者: exquisite 時(shí)間: 2025-3-22 06:13 作者: 新陳代謝 時(shí)間: 2025-3-22 09:20
Genome Engineering Using Adeno-Associated Virus (AAV),sease biology. These techniques fall into two categories based on the DNA repair mechanism that is used to incorporate the genetic change. Nuclease-based technologies, such as Zinc-Finger Nucleases, TALENS, and Crispr/Cas9, rely on non-homologous end-joining (NHEJ) and homology directed repair (HDR)作者: seroma 時(shí)間: 2025-3-22 16:38 作者: seroma 時(shí)間: 2025-3-22 18:21
Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform, domain and the development of TALE .ucleases (TALENs). TALENs enable researchers to make DNA double-strand breaks in target loci to create gene knockouts or introduce specific DNA sequence modifications. Precise genome engineering is increasingly being used to study gene function, develop disease m作者: 犬儒主義者 時(shí)間: 2025-3-23 00:53
Ligation-Independent Cloning (LIC) Assembly of TALEN Genes,s, e.g. using the . nuclease domain, provides a potent tool to induce DNA double strand breaks at user-defined genomic loci. In this regard, TAL (transcription activator-like) effector proteins, secreted by bacteria of the Xanthomonas family, provide the highest degree of modularity in their DNA bin作者: 現(xiàn)暈光 時(shí)間: 2025-3-23 03:49
Assembly and Characterization of megaTALs for Hyperspecific Genome Engineering Applications,TALs are novel monomeric nucleases composed of a site-specific meganuclease cleavage head with additional affinity and specificity provided by a TAL effector DNA binding domain. This fusion product facilitates the transformation of meganucleases into hyperspecific and highly active genome engineerin作者: bioavailability 時(shí)間: 2025-3-23 05:36 作者: Paradox 時(shí)間: 2025-3-23 13:14
Donor Plasmid Design for Codon and Single Base Genome Editing Using Zinc Finger Nucleases, virtually any location in the human genome within 50 bp of a desired site. Such high resolution ZFN engineering is well within the conversion tract limitations demarcated by the mammalian DNA repair machinery, resulting in a nearly universal ability to create point mutations throughout the human ge作者: 滋養(yǎng) 時(shí)間: 2025-3-23 14:42
Endogenous Gene Tagging with Fluorescent Proteins,ces in the desired locations in the genome of a cell can allow monitoring of endogenous activities of disease related genes. Native gene expression and regulation is preserved in these knock-in cells in contrast to cell lines with target overexpression under an exogenous promoter as in the case of t作者: 搏斗 時(shí)間: 2025-3-23 18:29
Silencing Long Noncoding RNAs with Genome-Editing Tools,anscriptome. However, detailed functional analysis lags behind their rapid discovery. This might be partially due to the lack of loss-of-function approaches that efficiently reduce the expression of these transcripts. Here, I describe a method that allows a specific and efficient targeting of the hi作者: Indict 時(shí)間: 2025-3-24 01:52 作者: mediocrity 時(shí)間: 2025-3-24 03:39 作者: 百靈鳥 時(shí)間: 2025-3-24 07:12 作者: 創(chuàng)新 時(shí)間: 2025-3-24 14:27
Using Engineered Endonucleases to Create Knockout and Knockin Zebrafish Models,argeted knockouts through the use of zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/CRISPR associated system (CRISPR/Cas). Furthermore, using the high-efficiency TALEN system, we were able to create作者: 偽證 時(shí)間: 2025-3-24 17:00
Creating Knockout and Knockin Rodents Using Engineered Endonucleases via Direct Embryo Injection, engineered endonucleases such as zinc-finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. These endonucleases are useful for simple and rapid production of gene knockout/knockin animals without 作者: rheumatism 時(shí)間: 2025-3-24 20:42 作者: 焦慮 時(shí)間: 2025-3-25 02:52
1064-3745 ible results.Contains key notes and implementation advice fr.This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols. Since the first edition, major advances 作者: Clinch 時(shí)間: 2025-3-25 04:22
Key Concepts in Chinese Thought and Cultureoaches that efficiently reduce the expression of these transcripts. Here, I describe a method that allows a specific and efficient targeting of the highly abundant lncRNA . in human (lung) cancer cells. The method relies on the site-specific integration of RNA-destabilizing elements mediated by Zinc Finger Nucleases (ZFNs).作者: antidote 時(shí)間: 2025-3-25 08:52
Redefining Chinese Literature and Art enrich for genome-edited cells such that they can be more easily identified. This chapter discusses several different approaches including the use of cold-shock, exonucleases, surrogate markers, specialized donor vectors, and oligonucleotides to enhance the frequency of genome editing or to facilitate the identification of genome-edited cells.作者: NUL 時(shí)間: 2025-3-25 12:44
https://doi.org/10.1007/978-1-4899-0961-9aced short palindromic repeats (CRISPR)-Cas9. These endonucleases are useful for simple and rapid production of gene knockout/knockin animals without using embryonic stem (ES) cells. This chapter introduces the latest protocols for producing genetically modified rodents using ZFN, TALEN, and CRISPR/Cas9.作者: notification 時(shí)間: 2025-3-25 16:42 作者: 相一致 時(shí)間: 2025-3-25 21:53
Strategies to Increase Genome Editing Frequencies and to Facilitate the Identification of Edited Ce enrich for genome-edited cells such that they can be more easily identified. This chapter discusses several different approaches including the use of cold-shock, exonucleases, surrogate markers, specialized donor vectors, and oligonucleotides to enhance the frequency of genome editing or to facilitate the identification of genome-edited cells.作者: 細(xì)菌等 時(shí)間: 2025-3-26 02:37 作者: 過去分詞 時(shí)間: 2025-3-26 08:06 作者: 議程 時(shí)間: 2025-3-26 11:37
Introduction: Political Cultures,g tools that are amenable to multiplexing and compatible with multiple cellular delivery methods. In this chapter, we describe the process of assembling a megaTAL from a meganuclease, as well as a method for characterization of nuclease cleavage activity in vivo using a fluorescence reporter assay.作者: 預(yù)感 時(shí)間: 2025-3-26 15:21 作者: Comprise 時(shí)間: 2025-3-26 19:13
Adele Eskeles Gottfried,Allen W. Gottfriedsing liquid nitrogen. This chapter introduces our latest protocols for freeze-drying of mouse and rat spermatozoa, and the anticipated results of the fertilizing ability of these gametes following long-term preservation or transportation.作者: podiatrist 時(shí)間: 2025-3-26 21:25
Book 2015Latest editiontheir respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and fully updated, .Chromosomal Mutagenesis, Second Edition. aims to help speed scientific discovery and aid in the next advances in the field.作者: 有毒 時(shí)間: 2025-3-27 04:01 作者: 側(cè)面左右 時(shí)間: 2025-3-27 08:16 作者: DEMN 時(shí)間: 2025-3-27 12:25
International and Development EducationEN construction based on one of the most popular TALEN assembly platforms, the Golden Gate cloning method. Using this protocol, ready-to-use TALENs with specificity for targets 13–32 bp long are constructed within 5 days.作者: 有法律效應(yīng) 時(shí)間: 2025-3-27 16:21 作者: Nebulous 時(shí)間: 2025-3-27 20:00
Using Phage Integrases in a Site-Specific Dual Integrase Cassette Exchange Strategy,integrases simultaneously to facilitate cassette exchange at a predefined location. This approach permits greater control and accuracy over integration. We also present a general method for using polymerase chain reaction assays to verify that the desired cassette exchange occurred successfully.作者: tinnitus 時(shí)間: 2025-3-27 22:39
Genome Engineering Using Adeno-Associated Virus (AAV),rations from gene knockouts, insertions of point mutations, indels, epitope tags, and reporter genes. Here we describe the generation and use of AAV gene targeting vectors and viruses to create targeted isogenic cell lines.作者: Cuisine 時(shí)間: 2025-3-28 02:17
Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform,EN construction based on one of the most popular TALEN assembly platforms, the Golden Gate cloning method. Using this protocol, ready-to-use TALENs with specificity for targets 13–32 bp long are constructed within 5 days.作者: insightful 時(shí)間: 2025-3-28 06:55 作者: 冷淡周邊 時(shí)間: 2025-3-28 14:20 作者: arterioles 時(shí)間: 2025-3-28 16:22
Introduction: Defining Transformations,s and . regulatory regions), which is feasible in an academic laboratory environment. A combination of two selection systems, in vitro compartmentalization and two-plasmid cleavage assay in bacteria, allow for efficient engineering of meganucleases that specifically cleave a wide variety of DNA sequences.作者: 保全 時(shí)間: 2025-3-28 22:05
Introduction: Political Cultures,an cells, verification of target cleavage, and assays for detecting genomic modification including indels and homologous recombination. These tools provide researchers with new instruments that accelerate both forward and reverse genetics efforts.作者: hankering 時(shí)間: 2025-3-29 02:59
Genome Editing by Targeted Chromosomal Mutagenesis,e efficiency of cleavage and the repair outcome depend on the biology of the particular system being addressed. These reagents are being used to introduce favorable characteristics into organisms of economic significance, and the prospects for enhancing human gene therapy appear very bright.作者: 燦爛 時(shí)間: 2025-3-29 04:39
Engineering of Customized Meganucleases via In Vitro Compartmentalization and In Cellulo Optimizatis and . regulatory regions), which is feasible in an academic laboratory environment. A combination of two selection systems, in vitro compartmentalization and two-plasmid cleavage assay in bacteria, allow for efficient engineering of meganucleases that specifically cleave a wide variety of DNA sequences.作者: 暫時(shí)中止 時(shí)間: 2025-3-29 09:04 作者: 草本植物 時(shí)間: 2025-3-29 12:11
Ligation-Independent Cloning (LIC) Assembly of TALEN Genes,ding mode. However, the assembly of large and highly repetitive TALE protein coding genes can be challenging. We describe a ligation-independent cloning (LIC) based method to allow high-throughput assembly of TALE nuclease genes at high fidelity and low effort and cost.作者: 思考才皺眉 時(shí)間: 2025-3-29 18:21
Assembly and Characterization of megaTALs for Hyperspecific Genome Engineering Applications,g tools that are amenable to multiplexing and compatible with multiple cellular delivery methods. In this chapter, we describe the process of assembling a megaTAL from a meganuclease, as well as a method for characterization of nuclease cleavage activity in vivo using a fluorescence reporter assay.作者: 類似思想 時(shí)間: 2025-3-29 21:43
Using Engineered Endonucleases to Create Knockout and Knockin Zebrafish Models, knockin zebrafish using a single-stranded DNA (ssDNA) protocol described here. Through the use of these technologies, the zebrafish has become a valuable vertebrate model and an excellent bridge between the invertebrate and mammalian model systems for the study of human disease.作者: 陳列 時(shí)間: 2025-3-30 03:44
Simple Sperm Preservation by Freeze-Drying for Conserving Animal Strains,sing liquid nitrogen. This chapter introduces our latest protocols for freeze-drying of mouse and rat spermatozoa, and the anticipated results of the fertilizing ability of these gametes following long-term preservation or transportation.作者: JAMB 時(shí)間: 2025-3-30 04:05
Book 2015Latest editiongh comprehensive coverage and detailed protocols. Since the first edition, major advances and discoveries have made chromosomal mutagenesis a widely used technique and one that is available to any molecular biology laboratory, and this collection provides detailed protocols, case-studies, and review作者: 脆弱帶來 時(shí)間: 2025-3-30 09:37
Money and Payments: From Beads to Bits,e designed to produce highly specific double-strand breaks in chromosomal DNA. These breaks are processed by cellular DNA repair machinery leading to localized mutations and to intentional sequence replacements. Because these repair processes are common to essentially all organisms, the targetable n作者: 記成螞蟻 時(shí)間: 2025-3-30 13:37
Ruth Wandh?fer,Hazem Danny Nakibst. In this approach, a genome-wide mutant library is first generated and then screened for a phenotype of interest. Subsequently, genes responsible for the phenotype are identified. There have been a number of successful screens in yeasts, . and .. These model organisms all allow loss-of-function m作者: Benzodiazepines 時(shí)間: 2025-3-30 20:22
Redefining A Philosophy for World GovernanceC31 integrase performs efficient recombination between its . site and either its own placed . site or a partially mismatched genomic pseudo . site. Bxb1 integrase, another large serine recombinase, has a similar level of recombinational activity, but recognizes only its own . and . sites. Previously作者: crumble 時(shí)間: 2025-3-30 22:21
Key Concepts in Chinese Thought and Cultureacellular homologous recombination affords the safety and efficacy profile suitable for such settings. Recombinagenic donor DNA and mutagenic triplex-forming molecules co-opt this natural recombination phenomenon to enable the specific, heritable editing and targeting of the genome. Editing the geno作者: 苦惱 時(shí)間: 2025-3-31 01:50 作者: extinguish 時(shí)間: 2025-3-31 05:32 作者: flaunt 時(shí)間: 2025-3-31 09:55 作者: occurrence 時(shí)間: 2025-3-31 16:13
Christopher S. Collins,Deane E. Neubauers, e.g. using the . nuclease domain, provides a potent tool to induce DNA double strand breaks at user-defined genomic loci. In this regard, TAL (transcription activator-like) effector proteins, secreted by bacteria of the Xanthomonas family, provide the highest degree of modularity in their DNA bin作者: 感情 時(shí)間: 2025-3-31 20:11 作者: 爆炸 時(shí)間: 2025-3-31 21:45 作者: 遍及 時(shí)間: 2025-4-1 04:04
Redefining Chinese Literature and Art virtually any location in the human genome within 50 bp of a desired site. Such high resolution ZFN engineering is well within the conversion tract limitations demarcated by the mammalian DNA repair machinery, resulting in a nearly universal ability to create point mutations throughout the human ge作者: 凝視 時(shí)間: 2025-4-1 07:31 作者: Campaign 時(shí)間: 2025-4-1 11:27 作者: 委托 時(shí)間: 2025-4-1 16:02
Redefining Chinese Literature and Artered regularly interspaced short palindromic repeats (CRISPR)/Cas9 nucleases, requires the creation of a targeted, chromosomal DNA double-stranded break (DSB). In mammalian cells, these DSBs are typically repaired by one of the two major DNA repair pathways: nonhomologous end joining (NHEJ) or homol作者: 討好女人 時(shí)間: 2025-4-1 22:02 作者: 才能 時(shí)間: 2025-4-2 02:33
Redefining Chinese Literature and Arten reported. Nonetheless, an important practical aspect of the strategy is develop methods to increase the frequency of genome editing or methods that enrich for genome-edited cells such that they can be more easily identified. This chapter discusses several different approaches including the use of作者: 小畫像 時(shí)間: 2025-4-2 04:31
Adele Eskeles Gottfried,Allen W. Gottfriedargeted knockouts through the use of zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/CRISPR associated system (CRISPR/Cas). Furthermore, using the high-efficiency TALEN system, we were able to create作者: 發(fā)微光 時(shí)間: 2025-4-2 08:42
https://doi.org/10.1007/978-1-4899-0961-9 engineered endonucleases such as zinc-finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. These endonucleases are useful for simple and rapid production of gene knockout/knockin animals without 作者: 口訣法 時(shí)間: 2025-4-2 13:59
Adele Eskeles Gottfried,Allen W. Gottfriedigerator at 4 °C. Furthermore, it is possible to realize easy and safe transportation of spermatozoa at an ambient temperature that requires neither liquid nitrogen nor dry ice. Freeze-drying spermatozoa has been established as a new method for storing genetic resources instead of cryopreservation u作者: 微粒 時(shí)間: 2025-4-2 16:02
Shondra M. Pruett-MillerIncludes all new cutting-edge methods and protocols for chromosomal mutagenesis research.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice fr作者: notice 時(shí)間: 2025-4-2 21:48
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/226333.jpg作者: IST 時(shí)間: 2025-4-3 02:59
Chromosomal Mutagenesis978-1-4939-1862-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
