標(biāo)題: Titlebook: Chromatin Protocols; Srikumar P. Chellappan Book 2015Latest edition Springer Science+Business Media New York 2015 Chromatin imaging.Chroma [打印本頁] 作者: iniquity 時間: 2025-3-21 18:53
書目名稱Chromatin Protocols影響因子(影響力)
書目名稱Chromatin Protocols影響因子(影響力)學(xué)科排名
書目名稱Chromatin Protocols網(wǎng)絡(luò)公開度
書目名稱Chromatin Protocols網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Chromatin Protocols被引頻次
書目名稱Chromatin Protocols被引頻次學(xué)科排名
書目名稱Chromatin Protocols年度引用
書目名稱Chromatin Protocols年度引用學(xué)科排名
書目名稱Chromatin Protocols讀者反饋
書目名稱Chromatin Protocols讀者反饋學(xué)科排名
作者: ESPY 時間: 2025-3-21 22:56 作者: ornithology 時間: 2025-3-22 04:07
Mathematical Details and Additional Physics,g the nucleosomal arrays to paramagnetic beads. Chromatin-containing beads can be retrieved from a reaction mix solution on a magnet fast and quantitatively, effectively separating bound, loosely attached and unbound components efficiently. This chapter details a convenient strategy for immobilizati作者: 輕觸 時間: 2025-3-22 08:38 作者: harpsichord 時間: 2025-3-22 09:52 作者: Pert敏捷 時間: 2025-3-22 15:01 作者: Pert敏捷 時間: 2025-3-22 18:45
Mathematical Details and Additional Physics,gnosis, or chromosome biology study. Chromosomes are usually prepared from mitotic cells arrested by colcemid block protocol. However, obtaining mitotic chromosomes is often hampered under several circumstances. As a result, cytogenetic analysis will be sometimes difficult or even impossible in such作者: 哎呦 時間: 2025-3-22 21:55 作者: Water-Brash 時間: 2025-3-23 03:53 作者: MOTIF 時間: 2025-3-23 06:42
https://doi.org/10.1007/978-3-642-13158-5the DNA template. The methylation status at different sites is dynamically regulated by histone methyltransferases and demethylases. During the past decade, two classes of proteins have been characterized to actively remove methyl groups from lysine residues through different mechanisms. The LSD1/KD作者: RAFF 時間: 2025-3-23 12:50
P. Suchomel,O. Choutka,P. Barsalls in a population may contribute to survival and disease, most assays of chromatin structure recover only population averages. We have developed DNA methyltransferases (MTases) as probing agents of DNA accessibility in chromatin, either expressed in vivo in budding yeast or as recombinant enzymati作者: biosphere 時間: 2025-3-23 15:27 作者: entice 時間: 2025-3-23 21:30
https://doi.org/10.1007/978-3-642-13158-5enomic methylation of cytosines within CpG dinucleotides is crucial to development, gene silencing, and chromosome inactivation. Importantly, aberrant methylation profiles of various genes are associated with cancer as well as autoimmune disease, psychiatric and neurodegenerative disorders, diabetes作者: debacle 時間: 2025-3-24 01:50
Urban Forests Prior to World War II,12; Zentner and Henikoff, Nat Struct Mol Biol 20:259–266, 2013). One example of such modifications is histone ubiquitination, which occurs predominately on H2A and H2B. Recent studies have highlighted important regulatory roles of H2A ubiquitination in Polycomb group protein-mediated gene silencing 作者: 慢跑鞋 時間: 2025-3-24 04:09 作者: 租約 時間: 2025-3-24 09:31
https://doi.org/10.1007/978-94-007-4396-0e is composed of core histones and DNA of variable size wrapped around it. Apart from the histone proteins, several nonhistone proteins also interact with the complex consisting of the DNA, the core and linker histones conferring highly regulated fluidity on the chromatin and permitting fine tuning 作者: Blatant 時間: 2025-3-24 11:04 作者: 預(yù)防注射 時間: 2025-3-24 17:43 作者: EXALT 時間: 2025-3-24 20:53 作者: FADE 時間: 2025-3-24 23:45 作者: 艦旗 時間: 2025-3-25 07:15
Masaki Hata,Jaehyun Song,Yutaka Shinada is key for regulating gene expression and cell functions. The extent to which the original chromatin structure is altered after repair of DNA lesions is thus a critical issue. Dissecting histone dynamics at sites of DNA damage has provided mechanistic insights into chromatin plasticity in response 作者: FRONT 時間: 2025-3-25 09:06
https://doi.org/10.1007/978-1-4939-2474-5Chromatin imaging; Chromatin structure and function; DNA methylation; Histone modifications; Non-histone作者: GENRE 時間: 2025-3-25 15:32
978-1-4939-4406-4Springer Science+Business Media New York 2015作者: refine 時間: 2025-3-25 15:49
Preparation and Analysis of Positioned Mononucleosomes,r processes, including binding of proteins to nucleosomes, covalent histone modifications, transcription, DNA repair, and ATP-dependent chromatin remodeling. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes assembled on 200–350-bp DNA fragments.作者: 他姓手中拿著 時間: 2025-3-25 22:38 作者: CANE 時間: 2025-3-26 02:56
Springer Tracts in Modern Physicsr processes, including binding of proteins to nucleosomes, covalent histone modifications, transcription, DNA repair, and ATP-dependent chromatin remodeling. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes assembled on 200–350-bp DNA fragments.作者: Misnomer 時間: 2025-3-26 06:45 作者: floaters 時間: 2025-3-26 09:40
Preparation and Analysis of Positioned Mononucleosomes,r processes, including binding of proteins to nucleosomes, covalent histone modifications, transcription, DNA repair, and ATP-dependent chromatin remodeling. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes assembled on 200–350-bp DNA fragments.作者: ANTIC 時間: 2025-3-26 13:16
Chromatin Imaging with Time-Lapse Atomic Force Microscopy,f high-speed AFM instrumentation made it possible to image the dynamics of proteins and protein-DNA complexes within millisecond time range. This chapter describes protocols for studies of structure and dynamics of nucleosomes with time-lapse AFM including the high-speed AFM instrument. The necessar作者: 用不完 時間: 2025-3-26 17:09
Isolation of Specific Genomic Regions and Identification of Associated Molecules by Engineered DNA-inding molecule-mediated chromatin immunoprecipitation (enChIP) using the CRISPR system, for purification of specific genomic regions retaining molecular interactions. In this form of enChIP, specific genomic regions are immunoprecipitated with antibody against a tag(s), which is fused to a catalyti作者: 碳水化合物 時間: 2025-3-26 22:10
Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Cgnosis, or chromosome biology study. Chromosomes are usually prepared from mitotic cells arrested by colcemid block protocol. However, obtaining mitotic chromosomes is often hampered under several circumstances. As a result, cytogenetic analysis will be sometimes difficult or even impossible in such作者: 清唱劇 時間: 2025-3-27 01:22 作者: 暗語 時間: 2025-3-27 06:17
Histone Deacetylase Activity Assay,terminal tail of histones. Accumulating evidence suggests that many, if not all, HDACs can also deacetylate nonhistone proteins. Through deacetylating histones and nonhistone proteins, HDACs regulate a variety of cellular processes including gene transcription, cell differentiation, DNA damage respo作者: BILIO 時間: 2025-3-27 09:26
In Vitro Histone Demethylase Assays,the DNA template. The methylation status at different sites is dynamically regulated by histone methyltransferases and demethylases. During the past decade, two classes of proteins have been characterized to actively remove methyl groups from lysine residues through different mechanisms. The LSD1/KD作者: FLINT 時間: 2025-3-27 16:41
Integrated DNA Methylation and Chromatin Structural Analysis at Single-Molecule Resolution,lls in a population may contribute to survival and disease, most assays of chromatin structure recover only population averages. We have developed DNA methyltransferases (MTases) as probing agents of DNA accessibility in chromatin, either expressed in vivo in budding yeast or as recombinant enzymati作者: dictator 時間: 2025-3-27 21:51
Determination of DNA Methylation Levels Using Illumina HumanMethylation450 BeadChips,plicated in development and disease. Several platforms are targeted toward the identification of DNA methylation changes that might be pertinent to the disease process and include regional analysis (e.g., pyrosequencing) as well as genome-wide analysis (e.g., next-generation sequencing and microarra作者: COST 時間: 2025-3-28 01:55
Investigation of Genomic Methylation Status Using Methylation-Specific and Bisulfite Sequencing Polenomic methylation of cytosines within CpG dinucleotides is crucial to development, gene silencing, and chromosome inactivation. Importantly, aberrant methylation profiles of various genes are associated with cancer as well as autoimmune disease, psychiatric and neurodegenerative disorders, diabetes作者: Torrid 時間: 2025-3-28 04:10 作者: 權(quán)宜之計 時間: 2025-3-28 08:01 作者: 單調(diào)性 時間: 2025-3-28 11:58
Identification and Characterization of Nonhistone Chromatin Proteins: Human Positive Coactivator 4 e is composed of core histones and DNA of variable size wrapped around it. Apart from the histone proteins, several nonhistone proteins also interact with the complex consisting of the DNA, the core and linker histones conferring highly regulated fluidity on the chromatin and permitting fine tuning 作者: 提名 時間: 2025-3-28 14:51 作者: hurricane 時間: 2025-3-28 20:13 作者: intellect 時間: 2025-3-29 01:17
Non-radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length,of telomerase in cancer and various tissues that harbors telomerase positive stem cells. This assay measures telomerase activity where the amount of target is dependent upon the activity of the enzyme. This protocol consists of two steps: first, telomeric repeats are added to the substrate by telome作者: 前面 時間: 2025-3-29 04:25
Detecting ATM-Dependent Chromatin Modification in DNA Damage Response,neration, immunodeficiency, and cancer. Ataxia-telangiectasia mutated (ATM) gene product belongs to the PI3K-like protein kinase (PIKKs) family and is functionally implicated in mitogenic signal transduction, chromosome condensation, meiotic recombination, cell-cycle control, and telomere maintenanc作者: finale 時間: 2025-3-29 09:18 作者: 魅力 時間: 2025-3-29 12:45 作者: 單純 時間: 2025-3-29 15:45 作者: CANON 時間: 2025-3-29 20:36 作者: 配置 時間: 2025-3-30 00:23 作者: 澄清 時間: 2025-3-30 05:25
Traumatic Atlantooccipital Dislocation (AOD)ow accurate assessment of differential methylation across large studies. This chapter outlines the laboratory methodologies associated with performing the Illumina Infinium Methylation Assay, including bisulfite conversion, whole-genome amplification, BeadChip hybridization, XStain procedures, and i作者: Interferons 時間: 2025-3-30 09:15 作者: packet 時間: 2025-3-30 13:28 作者: 有節(jié)制 時間: 2025-3-30 17:22
https://doi.org/10.1007/978-94-007-4396-0e H3 together with those to cell cycle specific markers will allow us to determine the nature of phosphorylation in a cell cycle-specific manner. In a complex system, such as tissue specimens, analysis using P-Histone H3 and a cell type specific antibody will allow identification of specific cells t作者: heterodox 時間: 2025-3-30 21:47
https://doi.org/10.1007/978-94-007-4396-0xample of a highly abundant chromatin protein, PC4 (Positive coactivator 4), we describe strategies for the identification of the chromatin-associated proteins and their structural and functional characterization.作者: V切開 時間: 2025-3-31 03:37
https://doi.org/10.1007/978-4-431-68464-0d or a lesion-specific endonuclease is available..Transcription-coupled repair (TC-NER) is a sub-pathway of NER that catalyzes the repair of the transcribed strand of active genes. RNA polymerase II is essential for TC-NER, and its occupancy on a damaged template can be analyzed by chromatin immunop作者: 不可思議 時間: 2025-3-31 08:08
Reconstruction of the Knee Jointlow a molecular dissection of the events underlying DNA replication control as it pertains to chromatin changes and accessibility. Here, we outline a novel experimental strategy for addressing the ability of specific proteins to induce large-scale chromatin unfolding (decondensation) in vivo upon si作者: CIS 時間: 2025-3-31 13:01 作者: 津貼 時間: 2025-3-31 16:14 作者: 分離 時間: 2025-3-31 18:03
Book 2015Latest editioned in a fourth section. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avo作者: BLA 時間: 2025-3-31 21:56 作者: inferno 時間: 2025-4-1 03:32 作者: MOAT 時間: 2025-4-1 07:54 作者: 防銹 時間: 2025-4-1 12:14 作者: Jejune 時間: 2025-4-1 17:19
Determination of DNA Methylation Levels Using Illumina HumanMethylation450 BeadChips,ow accurate assessment of differential methylation across large studies. This chapter outlines the laboratory methodologies associated with performing the Illumina Infinium Methylation Assay, including bisulfite conversion, whole-genome amplification, BeadChip hybridization, XStain procedures, and i作者: Magnitude 時間: 2025-4-1 21:21
Investigation of Genomic Methylation Status Using Methylation-Specific and Bisulfite Sequencing Polted or unmethylated DNA. Alternatively, amplification of both methylated and unmethylated DNA followed by sequencing can be used to detect methylation status at multiple CpG sites. The following chapter provides protocols for bisulfite conversion of DNA, methylation-specific PCR and bisulfite sequen作者: hardheaded 時間: 2025-4-1 22:47 作者: garrulous 時間: 2025-4-2 02:49 作者: hallow 時間: 2025-4-2 09:39
Identification and Characterization of Nonhistone Chromatin Proteins: Human Positive Coactivator 4 xample of a highly abundant chromatin protein, PC4 (Positive coactivator 4), we describe strategies for the identification of the chromatin-associated proteins and their structural and functional characterization.作者: 諂媚于人 時間: 2025-4-2 11:17
Methods to Study Transcription-Coupled Repair in Chromatin,d or a lesion-specific endonuclease is available..Transcription-coupled repair (TC-NER) is a sub-pathway of NER that catalyzes the repair of the transcribed strand of active genes. RNA polymerase II is essential for TC-NER, and its occupancy on a damaged template can be analyzed by chromatin immunop作者: 濃縮 時間: 2025-4-2 15:44
