標(biāo)題: Titlebook: Chromatin Accessibility; Methods and Protocol Georgi K. Marinov,William J. Greenleaf Book 2023 The Editor(s) (if applicable) and The Author [打印本頁] 作者: solidity 時間: 2025-3-21 18:15
書目名稱Chromatin Accessibility影響因子(影響力)
作者: 茁壯成長 時間: 2025-3-21 22:07 作者: 相互影響 時間: 2025-3-22 01:17 作者: 大量殺死 時間: 2025-3-22 04:38
The Islamic Doctrines of War And Peacegulation in the chromatins of eukaryotic cells. Here, we describe POP-seq (.rokaryotic chromatin .penness .rofiling .uencing), an adaptation of the ATAC-seq method, to interrogate changes in the openness of prokaryotic nucleoids.作者: Panther 時間: 2025-3-22 09:53
Identity and Psychiatric Disordersturned out to be immensely useful for identifying cREs genome-wide and tracking their dynamics across different cellular states and is the basis of numerous methods taking advantage of the preferential enzymatic cleavage/labeling of accessible DNA. ATAC-seq (.ssay for .ransposase-.ccessible .hromati作者: Harass 時間: 2025-3-22 16:38 作者: Harass 時間: 2025-3-22 17:40 作者: lesion 時間: 2025-3-22 23:57
https://doi.org/10.1057/9780230620988latory elements, such as enhancers and transcriptional start sites, are characterized by “open” or nucleosome-free regions. Understanding the areas of the genome that are not considered open chromatin has been more difficult. Protect-seq is a genomics technique that aims to identify inaccessible chr作者: 火車車輪 時間: 2025-3-23 03:15
The Islamic Doctrines of War And Peacegulation in the chromatins of eukaryotic cells. Here, we describe POP-seq (.rokaryotic chromatin .penness .rofiling .uencing), an adaptation of the ATAC-seq method, to interrogate changes in the openness of prokaryotic nucleoids.作者: 四海為家的人 時間: 2025-3-23 07:07
https://doi.org/10.1057/9780230620988ers chromatin composition and structure, and how accessible regions are affected by and recover from this disruption has been unclear. Here, we present repli-ATAC-seq, a protocol to profile accessible chromatin genome-wide on replicated DNA starting from 100,000 cells. In this method, replicated DNA作者: Anonymous 時間: 2025-3-23 13:15 作者: 傀儡 時間: 2025-3-23 17:53
Kristian J. Carlson,Damiano MarchiREs). In eukaryotes, these CREs are generally characterized by nucleosomal depletion and thus higher physical accessibility of DNA. Many methods exploit this property to map regions of high average accessibility, and thus putative active CREs, in bulk. However, these techniques do not provide inform作者: Headstrong 時間: 2025-3-23 18:20 作者: PHON 時間: 2025-3-24 00:19
Conclusion: Reconstructing Organization,cific gene regulatory programs in complex tissues. Paired-seq enables joint analysis of open chromatin and nuclear transcriptome from up to a million cells in parallel. It achieves ultra-high-throughput single-cell multiomics with the use of a combinatorial barcoding strategy involving sequential li作者: Acetaldehyde 時間: 2025-3-24 04:00 作者: 碎石 時間: 2025-3-24 07:59 作者: 蜈蚣 時間: 2025-3-24 12:21 作者: FRAUD 時間: 2025-3-24 16:26 作者: Excitotoxin 時間: 2025-3-24 21:46 作者: nullify 時間: 2025-3-24 23:46 作者: 刻苦讀書 時間: 2025-3-25 05:54 作者: Locale 時間: 2025-3-25 09:29 作者: 館長 時間: 2025-3-25 12:17
ATAC-See: A Tn5 Transposase-Mediated Assay for Detection of Chromatin Accessibility with ImagingAssay of transposase-accessible chromatin with visualization (ATAC-see), a transposase-mediated imaging technology that enables direct imaging of the accessible genome in situ and deep sequencing to reveal the identity of the imaged elements. Here we image spatial organization of the accessible genome in HT1080 cells with this method.作者: 規(guī)范要多 時間: 2025-3-25 16:01
Genome-Wide Mapping of Active Regulatory Elements Using ATAC-seqturned out to be immensely useful for identifying cREs genome-wide and tracking their dynamics across different cellular states and is the basis of numerous methods taking advantage of the preferential enzymatic cleavage/labeling of accessible DNA. ATAC-seq (.ssay for .ransposase-.ccessible .hromati作者: 補充 時間: 2025-3-25 23:54 作者: abolish 時間: 2025-3-26 02:57
Universal NicE-Seq: A Simple and Quick Method for Accessible Chromatin Detection in Fixed Cellsssible promoters, enhancers, nucleosome positioning, transcription factor occupancy, and other chromosomal protein binding. The starting biological materials are often fixed using formaldehyde crosslinking. Here, we describe accessible chromatin library preparation from low numbers of formaldehyde-c作者: Ejaculate 時間: 2025-3-26 08:11 作者: NAV 時間: 2025-3-26 10:53
Determination of the Chromatin Openness in Bacterial Genomesgulation in the chromatins of eukaryotic cells. Here, we describe POP-seq (.rokaryotic chromatin .penness .rofiling .uencing), an adaptation of the ATAC-seq method, to interrogate changes in the openness of prokaryotic nucleoids.作者: CRAMP 時間: 2025-3-26 14:21 作者: Oafishness 時間: 2025-3-26 19:36
Analysis of Chromatin Interaction and Accessibility by Trac-Loopingcture and its role in regulating cell activities. To examine the genome-wide chromatin interactions at accessible chromatin regions, we developed a DNA transposase-mediated analysis of chromatin looping (Trac-looping) method for simultaneously detecting chromatin interactions and chromatin accessibi作者: Obscure 時間: 2025-3-27 00:23 作者: avenge 時間: 2025-3-27 01:18 作者: 惰性氣體 時間: 2025-3-27 07:06 作者: 打擊 時間: 2025-3-27 10:38 作者: 招募 時間: 2025-3-27 14:28
Simultaneous Measurement of DNA Methylation and Nucleosome Occupancy in Single Cells Using scNOMe-Send nucleosome occupancy (i.e., chromatin accessibility) in single cells. scNOMe-seq combines the activity of a GpC Methyltransferase, an enzyme which methylates cytosines in GpC dinucleotides, with bisulfite conversion, whereby unmethylated cytosines are converted into thymines. Because GpC Methyltr作者: 廣大 時間: 2025-3-27 19:31
Massively Parallel Profiling of Accessible Chromatin and Proteins with ASAP-SeqA, additional measurements are required to characterize the multifaceted nature of eukaryotic cells. Here, we delineate the ATAC with Select Antigen Profiling by sequencing (ASAP-seq) protocol, a scalable approach to quantifying proteins via oligo-tagged antibodies alongside accessible DNA in thousa作者: Gustatory 時間: 2025-3-28 01:07 作者: 身體萌芽 時間: 2025-3-28 05:44 作者: 流出 時間: 2025-3-28 09:30 作者: Pander 時間: 2025-3-28 14:25
1064-3745 tocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and up-to-date, .Chromatin Accessibility: Methods and Protocols. serves as an 978-1-0716-2901-7978-1-0716-2899-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Digest 時間: 2025-3-28 16:32 作者: 情感 時間: 2025-3-28 20:24
Innovations in Monitoring Ocean History two orthogonal approaches to correct for this bias, the “corrected cut–uncut” and the “cut–all cut” method, so that accurate measurements of absolute accessibility or occupancy at restriction sites throughout a genome are possible. The protocol is presented for the example of . chromatin but may be作者: Immunotherapy 時間: 2025-3-29 02:46
https://doi.org/10.1057/9781403980168, (Buenrostro et al, Nat Methods 10:1213–1218, 2013) modify lysis conditions to permeabilize cells and mitochondria, and (Corces et al, Nat Methods 14:959–962, 2017) optimize bioinformatic processing protocols to collectively increase mitochondrial genome coverage for downstream analysis. Here, we d作者: 6Applepolish 時間: 2025-3-29 05:15 作者: lanugo 時間: 2025-3-29 09:16 作者: 飛鏢 時間: 2025-3-29 14:32 作者: 帶來 時間: 2025-3-29 17:24
1064-3745 expertsThis detailed volume provides a comprehensive resource covering the existing and state-of-the-art tools in the field of profiling chromatin accessibility and its dynamics. Beginning with a section on bulk-cell methods for profiling chromatin accessibility and nucleosome positioning that rely作者: Keratin 時間: 2025-3-29 23:17 作者: crucial 時間: 2025-3-30 01:31 作者: 鼓掌 時間: 2025-3-30 04:59
Mapping Nucleosome Location Using FS-Seq with next-generation sequencing to map the location of nucleosomes in chromatin. In this section, a procedure using a kit from New England Biolabs (NEB NEXT Ultra II FS DNA library prep Kit) to fragment chromatin in preparation for next-generation sequencing is described and compared to other available procedures for mapping nucleosome location.作者: slipped-disk 時間: 2025-3-30 09:11 作者: Medicaid 時間: 2025-3-30 15:33 作者: 一美元 時間: 2025-3-30 20:10 作者: 熱情贊揚 時間: 2025-3-30 22:58
American Doughboys and German ,,s to capture the dynamics governing methylation and accessibility vary across individual cells and cell types. Here, we provide a scalable plate-based protocol for preparing scNOMe-seq libraries from single nucleus suspensions.作者: antenna 時間: 2025-3-31 01:52
American Doughboys and German ,,tational modifications existing single-cell ATAC-seq workflows but yields distinct modalities underlying complex cellular states, including estimation of protein abundance on the cell surface as well as intracellular and intranuclear factors.作者: constellation 時間: 2025-3-31 08:12
Universal NicE-Seq: A Simple and Quick Method for Accessible Chromatin Detection in Fixed Cellsq (nicking enzyme-assisted sequencing). We also demonstrate a single tube method that enables direct NGS library preparation from low cell numbers without DNA purification. Furthermore, we demonstrated universal?NicE-seq on FFPE tissue section sample.作者: BATE 時間: 2025-3-31 11:07
Simultaneous Measurement of DNA Methylation and Nucleosome Occupancy in Single Cells Using scNOMe-Ses to capture the dynamics governing methylation and accessibility vary across individual cells and cell types. Here, we provide a scalable plate-based protocol for preparing scNOMe-seq libraries from single nucleus suspensions.作者: indices 時間: 2025-3-31 16:00
Massively Parallel Profiling of Accessible Chromatin and Proteins with ASAP-Seqtational modifications existing single-cell ATAC-seq workflows but yields distinct modalities underlying complex cellular states, including estimation of protein abundance on the cell surface as well as intracellular and intranuclear factors.
