標(biāo)題: Titlebook: Chromaffin Cells; Methods and Protocol Ricardo Borges Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to [打印本頁] 作者: 休耕地 時間: 2025-3-21 18:51
書目名稱Chromaffin Cells影響因子(影響力)
書目名稱Chromaffin Cells影響因子(影響力)學(xué)科排名
書目名稱Chromaffin Cells網(wǎng)絡(luò)公開度
書目名稱Chromaffin Cells網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Chromaffin Cells被引頻次
書目名稱Chromaffin Cells被引頻次學(xué)科排名
書目名稱Chromaffin Cells年度引用
書目名稱Chromaffin Cells年度引用學(xué)科排名
書目名稱Chromaffin Cells讀者反饋
書目名稱Chromaffin Cells讀者反饋學(xué)科排名
作者: 預(yù)知 時間: 2025-3-21 23:36
978-1-0716-2673-3The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: Militia 時間: 2025-3-22 02:14
Chromaffin Cells978-1-0716-2671-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 使厭惡 時間: 2025-3-22 06:58
https://doi.org/10.1007/978-3-319-68622-6cytosis of dense-core vesicles (DCVs). In this chapter, we describe a series of PC12 cell culture procedures optimized for real-time functional assays such as single-vesicle amperometry. In addition, these conditions have been widely used for single-cell biochemical assays such as the proximity ligation assay with immunostaining.作者: HOWL 時間: 2025-3-22 10:58
https://doi.org/10.1007/978-3-319-68622-6cytosis of dense-core vesicles (DCVs). In this chapter, we describe a series of PC12 cell culture procedures optimized for real-time functional assays such as single-vesicle amperometry. In addition, these conditions have been widely used for single-cell biochemical assays such as the proximity liga作者: 官僚統(tǒng)治 時間: 2025-3-22 13:27 作者: 官僚統(tǒng)治 時間: 2025-3-22 20:31
Leonid Grinin,Sergey Malkov,Andrey Korotayevisadvantages to the use of cultured cells for immunocytochemistry. One of the advantages is that cultured cells can be used for one or more weeks after the dissociation of cells, whereas one of the disadvantages is that the properties of cells in culture might change under artificial conditions. On 作者: 滲透 時間: 2025-3-22 21:50 作者: MAOIS 時間: 2025-3-23 01:45 作者: Occlusion 時間: 2025-3-23 09:28 作者: SPURN 時間: 2025-3-23 11:37 作者: 桶去微染 時間: 2025-3-23 15:30
https://doi.org/10.1057/9780230339378in their molecule chemical groups with the ability to yield (oxidize) or capture (reduce) electrons upon its exposure to an electrical field. In order to measure the secretion of catecholamines, they are oxidized at +650?mV with a carbon electrode, releasing every molecule of catecholamine that is o作者: bile648 時間: 2025-3-23 20:25
https://doi.org/10.1057/9780230339378al step in assessing the adrenal medullary tissue function. The difficult access to the gland in vivo allows only population activity to be recorded in this condition. In vitro preparations allow recordings of spontaneous or evoked activity from single or multiple cells, depending on the biological 作者: 噴出 時間: 2025-3-24 01:04
Liberalism and the Challenge of Pluralismmine (CA) release reported in adrenal glands from adult SHRs raised considerable interest for its possible implication in the genesis of hypertension. The use of powerful techniques such as calcium imaging, electrophysiology, and single-cell amperometry to monitor in real time the key steps in CA se作者: deficiency 時間: 2025-3-24 05:58
https://doi.org/10.1007/978-1-349-27445-1ation and shaping of the local and global cytosolic Ca. signals triggering the process of regulated exocytosis in chromaffin cells. Beyond that role, intraorganellar Ca. homeostasis also regulates organelle-specific processes such as oxidative phosphorylation in mitochondria, maturation of secretory作者: indemnify 時間: 2025-3-24 07:10
Liberalism and the Challenge of Pluralismmembrane fuses with the plasma membrane, which allows release of the stored content into the bloodstream or the surrounding tissue. Here, we give a detailed description of two complementary methods to observe and quantify exocytosis in single cells: high-resolution TIRF microscopy and patch-clamp ca作者: FOR 時間: 2025-3-24 14:00
Reconstituting Social Criticismling regulated exocytosis. This is in part due to the exquisite temporal resolution of the technique, and the possibility of combining quantification of exo-/endocytosis at the whole-cell level, with the ability to simultaneously monitor and control the calcium signals triggering vesicle fusion. In 作者: Heretical 時間: 2025-3-24 18:32
Philosophy, Contingency and Social Criticism, or serotonin, released by exocytosis from secretory cells..Amperometry has two main characteristics that make it ideal for the study of exocytosis at the single-cell level with single-vesicle resolution quantal release. (i) .. The carbon fiber microelectrode can be carefully positioned on plasma m作者: notification 時間: 2025-3-24 22:37
https://doi.org/10.1007/978-3-642-21572-8ir application to measuring the storage of neurotransmitters in cellular vesicles. We provide protocols to measure vesicle content, the release of catecholamines, and from there the fraction of transmitter released in each exocytosis event. The focus here has been a combination of methods to evaluat作者: Intuitive 時間: 2025-3-24 23:18
Rat Pheochromocytoma PC12 Cells in Culturecytosis of dense-core vesicles (DCVs). In this chapter, we describe a series of PC12 cell culture procedures optimized for real-time functional assays such as single-vesicle amperometry. In addition, these conditions have been widely used for single-cell biochemical assays such as the proximity ligation assay with immunostaining.作者: Detoxification 時間: 2025-3-25 06:18 作者: drusen 時間: 2025-3-25 09:40
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/226291.jpg作者: 人類 時間: 2025-3-25 13:02 作者: Contracture 時間: 2025-3-25 17:29
Adrenal Grafts in the Central Nervous System: Chromaffin and Chromaffin Progenitor Cell Transplantatanimal models and preclinical studies as a source for cell replacement therapy in Parkinson’s disease. The long-term cell survival of these cells in the nervous system is limited, and the observed motor improvements are highly variable. An alternative source for transplantation is chromaffin progeni作者: 食品室 時間: 2025-3-25 20:37 作者: Cpap155 時間: 2025-3-26 03:20 作者: Panther 時間: 2025-3-26 07:42
Immunogold for Protein Location in Chromaffin Cellsdate their contribution to cellular function within the adrenal gland and can be achieved only by immunoelectron microscopy. The most popular immunoelectron microscopic techniques involved gold particles conjugated to secondary antibodies, leading to electron-dense markers and the so-called immunogo作者: 混合,攙雜 時間: 2025-3-26 11:07
Visualization of Exo- and Endocytosis Membrane Dynamics with Super-Resolution STED Microscopych as fusion pore opening, pore expansion, constriction, and closure, as well as the membrane transformation from flat-shaped to round-shaped vesicles in real time. Here we depict a method of using the state-of-the-art STED microscopy to image these membrane dynamics in bovine chromaffin cells. This作者: landmark 時間: 2025-3-26 13:06 作者: 大方一點 時間: 2025-3-26 17:09
Real Time Recording of Perifused Chromaffin Cellsin their molecule chemical groups with the ability to yield (oxidize) or capture (reduce) electrons upon its exposure to an electrical field. In order to measure the secretion of catecholamines, they are oxidized at +650?mV with a carbon electrode, releasing every molecule of catecholamine that is o作者: 頭腦冷靜 時間: 2025-3-27 00:47
Recording of Chromaffin Cell Electrical Activity In Situ in Acute Adrenal Slicesal step in assessing the adrenal medullary tissue function. The difficult access to the gland in vivo allows only population activity to be recorded in this condition. In vitro preparations allow recordings of spontaneous or evoked activity from single or multiple cells, depending on the biological 作者: JADED 時間: 2025-3-27 01:23
Calcium Imaging and Amperometric Recording in Cultured Chromaffin Cells and Adrenal Slices from Normmine (CA) release reported in adrenal glands from adult SHRs raised considerable interest for its possible implication in the genesis of hypertension. The use of powerful techniques such as calcium imaging, electrophysiology, and single-cell amperometry to monitor in real time the key steps in CA se作者: peak-flow 時間: 2025-3-27 06:34 作者: 含水層 時間: 2025-3-27 13:22
Quantification of Secretory Granule Exocytosis by TIRF Imaging and Capacitance Measurementsmembrane fuses with the plasma membrane, which allows release of the stored content into the bloodstream or the surrounding tissue. Here, we give a detailed description of two complementary methods to observe and quantify exocytosis in single cells: high-resolution TIRF microscopy and patch-clamp ca作者: Phenothiazines 時間: 2025-3-27 14:20 作者: flavonoids 時間: 2025-3-27 20:28 作者: 小歌劇 時間: 2025-3-27 22:23
Vesicle Collision Protocols for the Study of Quantum Size and Exocytotic Fraction Releasedir application to measuring the storage of neurotransmitters in cellular vesicles. We provide protocols to measure vesicle content, the release of catecholamines, and from there the fraction of transmitter released in each exocytosis event. The focus here has been a combination of methods to evaluat作者: adj憂郁的 時間: 2025-3-28 03:53 作者: 和平主義 時間: 2025-3-28 06:56
Immunogold for Protein Location in Chromaffin Cellsbedding immunogold enables the simultaneous localization of different molecules in the cell using secondary antibodies conjugated with gold particles of different size. In this chapter, we introduce pre-embedding and post-embedding immunogold procedures used for the identification of quantitative ch作者: Contort 時間: 2025-3-28 10:54
Calcium Imaging and Amperometric Recording in Cultured Chromaffin Cells and Adrenal Slices from Normsiological relevance. Here, we describe the procedures to measure the blood pressure of rats in a noninvasive manner, how to obtain primary cultures of adrenal chromaffin cells and acute adrenal slices, and how to perform amperometric recordings and intracellular calcium imaging in these preparation作者: 懸掛 時間: 2025-3-28 16:17
Membrane Capacitance Measurements of Stimulus-Evoked Exocytosis in Adrenal Chromaffin Cellsof voltage-gated calcium channels to quantify stimulus-secretion coupling in chromaffin cells. Stimulus protocols developed for investigation of functionally distinct releasable vesicle pools are also described.作者: 包裹 時間: 2025-3-28 21:34 作者: Condense 時間: 2025-3-29 02:21 作者: RALES 時間: 2025-3-29 04:10 作者: Commonwealth 時間: 2025-3-29 07:56 作者: 帶來 時間: 2025-3-29 14:48 作者: 隱語 時間: 2025-3-29 15:56 作者: Irritate 時間: 2025-3-29 22:55 作者: forestry 時間: 2025-3-30 03:41 作者: 面包屑 時間: 2025-3-30 07:26 作者: chapel 時間: 2025-3-30 08:54
https://doi.org/10.1057/9780230339378xidized two electrons (e.) that are recorded as an electrical current. Amperometry is an easy-to-use and noninvasive technique for cells (unlike patch-clamp techniques for measuring membrane capacitance) and has been widely used to monitor online catecholamine release from perifused bovine chromaffin cell populations.作者: 輕快走過 時間: 2025-3-30 13:25
https://doi.org/10.1007/978-1-349-27445-1 granules, or stress in the endoplasmic reticulum. In this chapter, we describe current methods to study mitochondrial, endoplasmic reticulum, and secretory vesicle calcium homeostasis in living chromaffin cells using engineered targeted aequorins.作者: milligram 時間: 2025-3-30 17:46
Liberalism and the Challenge of Pluralismpacitance recordings. Precise stimulation of exocytosis is achieved by local pressure application or voltage-clamp depolarizations. While the chapter is focused on insulin-secreting cells as an accessible and disease-relevant model system, the methodology is applicable to a wide variety of secretory cells including chromaffin and PC12 cells.作者: 種植,培養(yǎng) 時間: 2025-3-30 20:42 作者: Limpid 時間: 2025-3-31 04:05
Introduction: Hoping for the Future,the steps necessary to process the adrenochromaffin tissue for the transmission electron microscopy; this protocol is suitable for any kind of adrenal tissue, and personally we used it in mammals, reptiles, and amphibians.作者: 反話 時間: 2025-3-31 07:30
The Future Society and the Transition to It in real time. Here we depict a method of using the state-of-the-art STED microscopy to image these membrane dynamics in bovine chromaffin cells. This method can potentially be applied to study other membrane structure dynamics in other cell model system.作者: FEAS 時間: 2025-3-31 12:48 作者: Fretful 時間: 2025-3-31 16:04
Transmission Electron Microscopy: A Method for Studying the Adrenal Chromaffin Cellsthe steps necessary to process the adrenochromaffin tissue for the transmission electron microscopy; this protocol is suitable for any kind of adrenal tissue, and personally we used it in mammals, reptiles, and amphibians.作者: 非實體 時間: 2025-3-31 18:46 作者: 花費 時間: 2025-3-31 22:33 作者: sclera 時間: 2025-4-1 03:00
Book 2023oubleshooting and avoiding known pitfalls..?..Authoritative and cutting-edge,?.Chromaffin Cells: Methods and Protocols .aims to be a?useful practical guide to researches to help further their study in this field.?.作者: harmony 時間: 2025-4-1 06:40
Book 2023 guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful?.Methods in Molecular Biology.?series format, chapters include
