標(biāo)題: Titlebook: Chimeric RNA; Methods and Protocol Hui Li,Justin Elfman Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 Gene f [打印本頁] 作者: microbe 時間: 2025-3-21 16:16
書目名稱Chimeric RNA影響因子(影響力)
書目名稱Chimeric RNA影響因子(影響力)學(xué)科排名
書目名稱Chimeric RNA網(wǎng)絡(luò)公開度
書目名稱Chimeric RNA網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Chimeric RNA被引頻次
書目名稱Chimeric RNA被引頻次學(xué)科排名
書目名稱Chimeric RNA年度引用
書目名稱Chimeric RNA年度引用學(xué)科排名
書目名稱Chimeric RNA讀者反饋
書目名稱Chimeric RNA讀者反饋學(xué)科排名
作者: negotiable 時間: 2025-3-22 00:02
Transportation of Radioactive Material,n of chimeric transcripts, especially when careful consideration is applied to experimental design. In this chapter, we provide guidelines and procedures for use of RT-PCR in detection and measurement of chimeric RNA expression.作者: reception 時間: 2025-3-22 03:18 作者: Creatinine-Test 時間: 2025-3-22 08:00 作者: arsenal 時間: 2025-3-22 10:43 作者: objection 時間: 2025-3-22 16:02
1064-3745 expertsThis book provides numerous methods for identification, validation, and functional characterization of chimeric RNAs, herein described as any transcript which contains the nucleotide sequence of two distinct parental genes. Beginning with identification and characterization through bioinform作者: objection 時間: 2025-3-22 20:24 作者: Absenteeism 時間: 2025-3-23 00:19 作者: insert 時間: 2025-3-23 02:55
https://doi.org/10.1007/978-981-19-4518-2neral methodology used in processing and filtering of RNA-seq data, followed by filtering of multiple varieties of artifacts to nominate potentially relevant gene fusions. Functional relevance of gene fusions is assessed based on the predicted domain architecture of the putative fusion proteins.作者: 賞心悅目 時間: 2025-3-23 08:45 作者: 亞麻制品 時間: 2025-3-23 13:02
Dosimetry of Radiolabeled Antibodies, assay to detect transcriptional read-through. Here, we describe a general RT-PCR based method to confirm the process for cis-SAGe candidates. In this method, we use PCR to amplify cDNA that is reverse transcribed from the read-through precursor mRNA. The result provides a foundation for further downstream mechanistic studies.作者: Arbitrary 時間: 2025-3-23 17:13
Prediction, Characterization, and In Silico Validation of Chimeric RNAscribe the detailed steps for (1) prediction of chimeric RNAs using EricScript software, (2) characterization of chimeric RNAs to discard most probable false positive events, and (3) in silico validation of chimeric RNA to select the potential cancer-specific events.作者: 陰險 時間: 2025-3-23 18:47 作者: 隱藏 時間: 2025-3-23 23:26 作者: Obscure 時間: 2025-3-24 03:53
Detection of Group II Intron-Generated Chimeric mRNAs in Bacterial Cellsenerate chimeric mRNAs through a novel intergenic .-splicing pathway. Here we describe the detailed experimental procedures to detect group II intron-generated mRNA–mRNA chimeras from total RNA extracts using stringent reverse transcription conditions along with a reverse splicing-deficient group II intron as a negative control.作者: Lament 時間: 2025-3-24 08:52 作者: Interregnum 時間: 2025-3-24 14:27
Book 2020tep, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Chimeric RNA: Methods and Protocols. is an ideal guide to this expansive field and an inspiration to researchers seeking to further our knowledge of these remarkable features of the transcriptome..作者: Decibel 時間: 2025-3-24 15:54
Identification of Chimeric RNAs Using RNA-Seq Reads and Protein–Protein Interactions of Translated Cs using RNA-seq reads. Moreover, we elaborate on the domain–domain co-occurrence method to detect alterations in chimeric protein–protein interaction (ChiPPI) networks produced by chimeric RNA that are translated to chimeric proteins.作者: 蝕刻 時間: 2025-3-24 21:19
Detection and Measurement of Chimeric RNAs by RT-PCRn of chimeric transcripts, especially when careful consideration is applied to experimental design. In this chapter, we provide guidelines and procedures for use of RT-PCR in detection and measurement of chimeric RNA expression.作者: 絕食 時間: 2025-3-25 01:42
Validation of Chimeric Fusion Peptides Using Proteomics Datacribe the detailed steps for (1) downloading and processing publicly available proteomics datasets, (2) developing fusion peptide database by performing in silico tryptic digestion of chimeric proteins, and (3) software used to identify chimeric peptides in the proteomics data.作者: SPASM 時間: 2025-3-25 06:38
NanoString nCounter Technology: High-Throughput RNA Validationation, single nucleotide variation and chimeric RNAs can be analyzed. In this chapter, we discuss the design, setup, and performance of a NanoString-based RNA assay for gene expression or fusion transcript assessment.作者: MOT 時間: 2025-3-25 08:27 作者: PRO 時間: 2025-3-25 13:42
Transport of Radioactive Materials,e driving the development of the INTEGRATE tool and describe the detailed practical procedures for applying INTEGRATE to discover gene fusions using NGS data. INTEGRATE can be applied to both combined data and RNA-seq only data.作者: ARCHE 時間: 2025-3-25 17:23 作者: coagulation 時間: 2025-3-25 23:48
Ravinder Nath PhD,M. Saiful Huq PhDof chimeric RNAs in these fractions. Within this chapter we describe the most frequently used cellular fractionation technique: the separation of the cytoplasmic fraction from the nuclear fraction in a cell.作者: Intact 時間: 2025-3-26 01:37 作者: BUST 時間: 2025-3-26 05:45
RNase Protection Assay can bind specifically with the target chimeric RNA in order to form double-stranded RNA. This newly formed RNA is resistant to digestion by RNase and therefore can be identified by high-resolution, denaturing polyacrylamide gel electrophoresis.作者: CLAM 時間: 2025-3-26 12:26 作者: Flu表流動 時間: 2025-3-26 16:01 作者: 船員 時間: 2025-3-26 17:08
Overexpression of Chimeric RNA by Retroviral Transductionion of the chimeric RNA. Here, we provide procedures for the insertion of the . fusion ORF into a mammalian expression vector, achieving the overexpression of the . fusion protein in a colon cancer cell line by retroviral transduction.作者: 虛情假意 時間: 2025-3-26 22:14
https://doi.org/10.1007/978-1-4939-9904-0Gene fusion; Transcription-mediated fusions; Fusion transcripts; Bioinformatics; Translational applicati作者: 哄騙 時間: 2025-3-27 03:49
978-1-4939-9906-4Springer Science+Business Media, LLC, part of Springer Nature 2020作者: aerial 時間: 2025-3-27 07:17
EFFECTS OF CONTAMINANT EXPOSURE ON PLANTSpackages predict a large number of false positive events, which significantly hampers experimental validation of predicted chimeric RNAs. Here, we describe the detailed steps for (1) prediction of chimeric RNAs using EricScript software, (2) characterization of chimeric RNAs to discard most probable作者: 抵制 時間: 2025-3-27 11:49 作者: enmesh 時間: 2025-3-27 14:30 作者: ROOF 時間: 2025-3-27 19:34
Transport of Radioactive Materials,ity of patients with whole transcriptome sequencing (RNA-seq) and whole genome sequencing (WGS) data. We developed a gene fusion discovery tool, INTEGRATE, that leverages both RNA-seq and WGS data to reconstruct gene fusion junctions and genomic breakpoints by split-read alignment. INTEGRATE has bec作者: configuration 時間: 2025-3-28 00:11 作者: 和音 時間: 2025-3-28 03:06
Transportation of Radioactive Material,scripts. RT-PCR consists of RNA isolation and reverse transcription into complementary DNA (cDNA), which can then be used as input to a variety of PCR-based procedures such as standard PCR, real-time or quantitative PCR (qPCR or qRT-PCR), or TaqMan PCR procedures. These assays are useful in detectio作者: mitten 時間: 2025-3-28 10:09 作者: Alcove 時間: 2025-3-28 11:11
Biological Effects of Radiation in Humans,s principle, the RNase Protection Assay (RPA) is used to validate chimeric RNAs. Importantly, this assay does not employ reverse transcription (RT), thus avoiding potential false-positive results which could occur during RT such as template-switching. We first generate RNA probes with .phosphate (P)作者: 高腳酒杯 時間: 2025-3-28 18:34
Transportation of Radioactive Material, algorithms have been developed to identify chimeric RNAs, however, identification and validation of fused protein product of the chimeric RNA is still an emerging field. These chimeric proteins can be validated by searching and identifying them in publicly available proteomics datasets. Here we des作者: 不透明性 時間: 2025-3-28 19:56 作者: 殺子女者 時間: 2025-3-29 02:23
Radiation Theory and the Quantum Revolutioneduce the expression of a target gene by degrading its mRNA with short hairpin RNAs (shRNAs) or short interfering RNAs (siRNAs). Knockdown of chimeric RNAs present different challenges than standard RNAi targeting for regular genes. Most specifically, sequence homology restricts the targeting region作者: atrophy 時間: 2025-3-29 05:38 作者: AORTA 時間: 2025-3-29 09:59 作者: 音的強(qiáng)弱 時間: 2025-3-29 14:42 作者: 手術(shù)刀 時間: 2025-3-29 18:55 作者: 凌辱 時間: 2025-3-29 21:30
Hui Li,Justin ElfmanIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: 樹木中 時間: 2025-3-30 03:21
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/225311.jpg作者: Expand 時間: 2025-3-30 07:55
Chimeric RNA978-1-4939-9904-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: glowing 時間: 2025-3-30 12:15 作者: 勤勉 時間: 2025-3-30 13:19
Prediction, Characterization, and In Silico Validation of Chimeric RNAspackages predict a large number of false positive events, which significantly hampers experimental validation of predicted chimeric RNAs. Here, we describe the detailed steps for (1) prediction of chimeric RNAs using EricScript software, (2) characterization of chimeric RNAs to discard most probable作者: 密碼 時間: 2025-3-30 18:01
Identification of Fusion Transcripts from Unaligned RNA-Seq Reads Using ChimeRScopearkers. Several algorithms have been developed to predict fusion transcripts from DNA or RNA data. The majority of these algorithms align sequencing reads to the reference transcriptome for predicting fusions; however, this results in several undetected fusions due to the highly perturbed nature of 作者: preeclampsia 時間: 2025-3-30 23:29 作者: forestry 時間: 2025-3-31 01:42 作者: nascent 時間: 2025-3-31 08:14 作者: Awning 時間: 2025-3-31 10:22
Detection and Measurement of Chimeric RNAs by RT-PCRscripts. RT-PCR consists of RNA isolation and reverse transcription into complementary DNA (cDNA), which can then be used as input to a variety of PCR-based procedures such as standard PCR, real-time or quantitative PCR (qPCR or qRT-PCR), or TaqMan PCR procedures. These assays are useful in detectio作者: 基因組 時間: 2025-3-31 16:33 作者: affinity 時間: 2025-3-31 21:27 作者: Demonstrate 時間: 2025-3-31 23:29
Validation of Chimeric Fusion Peptides Using Proteomics Data algorithms have been developed to identify chimeric RNAs, however, identification and validation of fused protein product of the chimeric RNA is still an emerging field. These chimeric proteins can be validated by searching and identifying them in publicly available proteomics datasets. Here we des
