作者: Mhc-Molecule 時(shí)間: 2025-3-21 21:51
Clonogenic Cell Survival Assay,large colony or a clone. This cell is then said to be clonogenic. A cell survival curve is therefore defined as a relationship between the dose of the agent used to produce an insult and the fraction of cells retaining their ability to reproduce. Although clonogenic cell survival assays were initial作者: Comedienne 時(shí)間: 2025-3-22 00:30
High-Sensitivity Cytotoxicity Assays for Nonadherent Cells,ingle remaining viable cell. Two such assays are described here, which have been used with Raji B-lymphoma cells, and are applicable to other nonadherent target cells. The first is a cell-counting assay, performed over a 3-wk period, which provides a simple, reliable, and sensitive assay of cytotoxi作者: 逗它小傻瓜 時(shí)間: 2025-3-22 08:27
Sulforhodamine B Assay and Chemosensitivity,ug-screening applications. Its principle is based on the ability of the protein dye sulforhodamine B to bind electrostatically and pH dependent on protein basic amino acid residues of trichloroacetic acid-fixed cells. Under mild acidic conditions it binds to and under mild basic conditions it can be作者: sultry 時(shí)間: 2025-3-22 12:09
Use of the Differential Staining Cytotoxicity Assay to Predict Chemosensitivity,ations have demonstrated the clinical value of chemosensitivity testing with that assay (and similar ones). The DiSC assay is successful in the majority of malignant samples of which the cells can be brought in suspension (not necessarily as single-cell suspension). Although the assay is laborious a作者: 名次后綴 時(shí)間: 2025-3-22 16:11
Collagen Gel Droplet Culture Method to Examine In Vitro Chemosensitivity,ancer. We have developed a new in vitro chemosensitivity test named the collagen gel droplet embedded culture drug sensitivity test (CD-DST). The CD-DST method consists of a collagen gel droplet embedded culture step, exposure and washout of anticancer drug, a serum-free culture step, and evaluation作者: 名次后綴 時(shí)間: 2025-3-22 19:54
The MTT Assay to Evaluate Chemosensitivity,r cytokines on both normal and malignant cell populations. This can be accomplished by either direct or indirect counting methods. Direct counting by manual or automated methods, using a hemacytometer or particle counter, respectively, allows for serial cell counting at multiple time points, but the作者: Benign 時(shí)間: 2025-3-22 22:48
Histoculture Drug Response Assay to Monitor Chemoresponse,lium bromide (MTT) end point among several modified HDRA procedures. Fresh surgical specimens are cut into approx 1- to 2-mm. pieces and put on a gelatin sponge infiltrated with culture medium containing a test drug. After incubation for 7 d, cell viability is assessed by the MTT assay. HDRA uses ca作者: Arthr- 時(shí)間: 2025-3-23 05:28 作者: 阻塞 時(shí)間: 2025-3-23 05:33 作者: conjunctivitis 時(shí)間: 2025-3-23 12:21 作者: GUILE 時(shí)間: 2025-3-23 13:54 作者: EWER 時(shí)間: 2025-3-23 21:53 作者: EXUDE 時(shí)間: 2025-3-24 00:25 作者: 無(wú)聊點(diǎn)好 時(shí)間: 2025-3-24 05:39 作者: 角斗士 時(shí)間: 2025-3-24 08:20
Immunohistochemical Detection of Ornithine Decarboxylase as a Measure of Chemosensitivity Testing,er treatment. The development of individualized chemotherapy could minimize undesired toxic side effects and increase the chance of recovery. The known methods for in vitro chemosensitivity tests are mainly based on monitoring the metabolic changes induced in cancer cells by the drugs. These experim作者: affluent 時(shí)間: 2025-3-24 13:31
Immunohistochemistry of p53, Bcl-2, and Ki-67 as Predictors of Chemosensitivity,n. We analyzed immunohistochemically the expression of p53, Bcl-2, and Ki-67 in various types of cancers and assessed the correlation between this expression and chemosensitivity. Moreover, we investigated whether the expression of these factors could be a useful predictor for the clinical response 作者: beta-cells 時(shí)間: 2025-3-24 15:47 作者: GENRE 時(shí)間: 2025-3-24 19:16 作者: 追逐 時(shí)間: 2025-3-25 02:13
Expansion Microscopy for Brain Imaging responsiveness, development of drug resistance and induction of apoptosis, in vivo tumor growth and survival assays in metastatic and orthotopic models, and in vivo imaging assays. The advantages and disadvantages of the specific assays are discussed. A summary of research areas related to chemosensitivity testing is also included.作者: Foam-Cells 時(shí)間: 2025-3-25 06:23 作者: commensurate 時(shí)間: 2025-3-25 08:11 作者: 朋黨派系 時(shí)間: 2025-3-25 14:34 作者: 烤架 時(shí)間: 2025-3-25 18:48 作者: 機(jī)制 時(shí)間: 2025-3-25 20:11
The MTT Assay to Evaluate Chemosensitivity,mpound 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) has been the most widely utilized. Newer tetrazolium dyes that yield water-soluble products and offer added flexibility, increases in sensitivity, and fewer steps, which are offset by increased costs, are also covered.作者: 用肘 時(shí)間: 2025-3-26 01:56
Book 2005otechnology. A companion volume, Volume 2: In Vivo Models, Imaging, and Molecular Regulators, provides protocols for classifying tumors into response categories and customizing chemotherapy regimens to individual patients.作者: eucalyptus 時(shí)間: 2025-3-26 05:38 作者: Psa617 時(shí)間: 2025-3-26 11:51
Raman and Infrared (IR) Spectroscopy,een malignant and contaminating nonmalignant cells. The latter is a major advantage of the DiSC assay. This chapter describes the practical aspects of this assay, several topics that need to be taken into account, and potential pitfalls. As such, it is not an extensive review of studies in which the DiSC assay was used.作者: 不妥協(xié) 時(shí)間: 2025-3-26 16:09 作者: 太空 時(shí)間: 2025-3-26 18:43 作者: 抗體 時(shí)間: 2025-3-26 22:58 作者: Tremor 時(shí)間: 2025-3-27 05:04
Advanced Parallel Processing Technologiesombination than expected from the sum of each agent alone). Here, we briefly review some of the principles for testing cytotoxic drug interactions. We focus this review on application of the Combination Index method (as developed by Chou and colleagues) in the evaluation of drug interactions in cell culture assays.作者: Harass 時(shí)間: 2025-3-27 06:15
Use of the Differential Staining Cytotoxicity Assay to Predict Chemosensitivity,een malignant and contaminating nonmalignant cells. The latter is a major advantage of the DiSC assay. This chapter describes the practical aspects of this assay, several topics that need to be taken into account, and potential pitfalls. As such, it is not an extensive review of studies in which the DiSC assay was used.作者: 細(xì)絲 時(shí)間: 2025-3-27 12:38
Collagen Gel Droplet Culture Method to Examine In Vitro Chemosensitivity,number of cells for the test, easy quantification of the anticancer effects without contamination with fibroblasts by using an image analysis system, a good correlation between in vitro and in vivo results, and simplicity and speed. The CD-DST method can be performed in the laboratory using a system kit Primaster?.作者: drusen 時(shí)間: 2025-3-27 14:30
In Vitro Testing of Chemosensitivity in Physiological Hypoxia,oved by conducting testing in conditions of physiological hypoxia. We review the impact of hypoxia on anticancer drug cytotoxicity and the methods used in our laboratory to asses the cytotoxic activity of single antineoplastic drugs under conditions of physiological hypoxia.作者: 楓樹(shù) 時(shí)間: 2025-3-27 19:23 作者: 侵略主義 時(shí)間: 2025-3-28 00:19 作者: 慢跑鞋 時(shí)間: 2025-3-28 02:37
1543-1894 vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Chemosensitivity, Volume 1: In Vitro Assays provides a panel of 16 in vitro measures of chemosensitivity in adherent and non-adherent cells for single agents and combinations of agents. In additi作者: 傲慢人 時(shí)間: 2025-3-28 09:10 作者: 合同 時(shí)間: 2025-3-28 12:29
Sulforhodamine B Assay and Chemosensitivity,assays such as MTT or clonogenic assay. The SRB assay possesses a colorimetric end point and is nondestructive and indefinitely stable. These practical advances make the SRB assay an appropriate and sensitive assay to measure drug-induced cytotoxicity even at large-scale application.作者: 摻假 時(shí)間: 2025-3-28 16:28
Chemosensitivity Testing Using Microplate Adenosine Triphosphate-Based Luminescence Measurements, technique; low technical failure rates; and a high methodological efficacy requiring only 1 × 10. tumor cells to test four to six different drugs or combinations. In ovarian and breast carcinomas, the predictive accuracy is <90%, with a positive predictive value of 85-90‰ and a negative predictive 作者: 作繭自縛 時(shí)間: 2025-3-28 22:25 作者: 使迷醉 時(shí)間: 2025-3-28 23:53 作者: archaeology 時(shí)間: 2025-3-29 06:39
Image Analysis Using the Fluorochromasia Assay to Quantify Tumor Drug Sensitivity,10, 30, or 100 MNC-190. The potential benefit of this technique is the ability to predict the response of tumors to chemotherapeutic agents as a laboratory tool for preclinical drug evaluation and clinically prior to the commencement of therapy.作者: 四溢 時(shí)間: 2025-3-29 09:47 作者: 郊外 時(shí)間: 2025-3-29 11:33
Immunohistochemistry of p53, Bcl-2, and Ki-67 as Predictors of Chemosensitivity,ponse assay method using four drugs: mitomycin C, 5-fluorouracil, doxorubicin hydrochloride (ADM), and cisplatin (CDDP). Immunohistochemical studies for p53 were found to be useful for predicting chemosensitivity.作者: 大方一點(diǎn) 時(shí)間: 2025-3-29 17:08 作者: 說(shuō)不出 時(shí)間: 2025-3-29 21:05
Adaptive Optics in Multiphoton Microscopyrvival curves have been generated for many established cell lines growing in culture. One can use cell lines from various origins including humans and rodents; these cells can be neoplastic or normal. Because survival curves have wide application in evaluating the reproductive integrity of different作者: FLORA 時(shí)間: 2025-3-30 00:42
Fu-Jen Kao,Gerd Keiser,Ankur Gogoiassays such as MTT or clonogenic assay. The SRB assay possesses a colorimetric end point and is nondestructive and indefinitely stable. These practical advances make the SRB assay an appropriate and sensitive assay to measure drug-induced cytotoxicity even at large-scale application.作者: jeopardize 時(shí)間: 2025-3-30 04:33
K. F. Wang,Zhenzhou Ji,Mingzeng Hu technique; low technical failure rates; and a high methodological efficacy requiring only 1 × 10. tumor cells to test four to six different drugs or combinations. In ovarian and breast carcinomas, the predictive accuracy is <90%, with a positive predictive value of 85-90‰ and a negative predictive 作者: Stable-Angina 時(shí)間: 2025-3-30 10:28 作者: 鐵砧 時(shí)間: 2025-3-30 13:21
https://doi.org/10.1007/978-981-99-7872-4 or antagonistic interactions. The linearity of DIMSCAN was validated (. . = 0.99967 ± 0.0003) for cells stained with FDA deposited using a fluorescence-activated cell sorter, documenting a dynamic range>4 logs, and the ability to detect a single viable cell in a well 93‰ of the time. DIMSCAN has be作者: JEER 時(shí)間: 2025-3-30 19:06 作者: 壓艙物 時(shí)間: 2025-3-30 21:17
Lecture Notes in Computer Science Because ODC has an extremely short half-life, it disappears when cellular proliferation is arrested. The decay of ODC was assayed both by determining its activity and by immunohistochemical analyses. This approach was successfully used to determine the sensitivity of lymphocytes from hematological 作者: 蠟燭 時(shí)間: 2025-3-31 03:53
Lecture Notes in Computer Scienceponse assay method using four drugs: mitomycin C, 5-fluorouracil, doxorubicin hydrochloride (ADM), and cisplatin (CDDP). Immunohistochemical studies for p53 were found to be useful for predicting chemosensitivity.作者: dyspareunia 時(shí)間: 2025-3-31 08:14
Rosalyn D. BlumenthalIncludes supplementary material: 作者: 數(shù)量 時(shí)間: 2025-3-31 11:58 作者: expeditious 時(shí)間: 2025-3-31 14:39 作者: CHOKE 時(shí)間: 2025-3-31 19:55 作者: 內(nèi)部 時(shí)間: 2025-4-1 00:54
Expansion Microscopy for Brain Imagingingle remaining viable cell. Two such assays are described here, which have been used with Raji B-lymphoma cells, and are applicable to other nonadherent target cells. The first is a cell-counting assay, performed over a 3-wk period, which provides a simple, reliable, and sensitive assay of cytotoxi作者: 知識(shí)分子 時(shí)間: 2025-4-1 02:51
Fu-Jen Kao,Gerd Keiser,Ankur Gogoiug-screening applications. Its principle is based on the ability of the protein dye sulforhodamine B to bind electrostatically and pH dependent on protein basic amino acid residues of trichloroacetic acid-fixed cells. Under mild acidic conditions it binds to and under mild basic conditions it can be
