標題: Titlebook: Chemogenomics; Methods and Protocol Daniel Merk,Apirat Chaikuad Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive [打印本頁] 作者: 贖罪 時間: 2025-3-21 17:55
書目名稱Chemogenomics影響因子(影響力)
書目名稱Chemogenomics影響因子(影響力)學(xué)科排名
書目名稱Chemogenomics網(wǎng)絡(luò)公開度
書目名稱Chemogenomics網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Chemogenomics被引頻次
書目名稱Chemogenomics被引頻次學(xué)科排名
書目名稱Chemogenomics年度引用
書目名稱Chemogenomics年度引用學(xué)科排名
書目名稱Chemogenomics讀者反饋
書目名稱Chemogenomics讀者反饋學(xué)科排名
作者: EVICT 時間: 2025-3-21 21:43
Queueing Theory and Network Applications using small-molecule antagonists and genetic mutations. Here, we provide a detailed protocol for expressing NanoLuc and HaloTag fusion proteins in cell culture and the necessary optimization of NanoBRET assay conditions. Our example results demonstrate the reliability and sensitivity of NanoBRET fo作者: 蒙太奇 時間: 2025-3-22 03:01 作者: GROSS 時間: 2025-3-22 07:14 作者: ineluctable 時間: 2025-3-22 11:11 作者: CARE 時間: 2025-3-22 14:03 作者: CARE 時間: 2025-3-22 20:48
Elvio Gilberto Amparore,Susanna Donatelliy study the response of a biological system to a set of compounds, which can aid the identification and validation of biological targets as well as biologically active small-molecule agents responsible for a phenotypic outcome. Central to this strategy is a collection of chemically diverse compounds作者: Orgasm 時間: 2025-3-22 23:25 作者: corpus-callosum 時間: 2025-3-23 02:45
https://doi.org/10.1007/978-3-319-66637-2 search. Nonetheless, owning to nonuniform data mining, these databases are often incomplete, thus advocating the combined use of data from several repositories to increase target coverage and data accuracy. Here, we present a workflow to generate custom datasets from public databases for mining che作者: Biofeedback 時間: 2025-3-23 05:38 作者: hauteur 時間: 2025-3-23 13:12 作者: 柱廊 時間: 2025-3-23 15:28 作者: 文件夾 時間: 2025-3-23 19:26 作者: 松雞 時間: 2025-3-23 23:59 作者: 開玩笑 時間: 2025-3-24 06:03
Quan-Lin Li,Rui-Na Fan,Zhi-Yong Qianciferase-based techniques require expensive substrates and are typically performed in endpoint format. Here, we describe a versatile reporter gene assay to observe nuclear receptor activity with fluorescent proteins as reporters. This setting is highly cost-efficient and enables observation of nucle作者: impaction 時間: 2025-3-24 09:39 作者: EXUDE 時間: 2025-3-24 11:47
Lecture Notes in Computer Sciencesure a compound’s cellular target engagement and permeability. HiBiT CETSA method is quantitative and has higher throughput compared to the traditional Western-based CETSA. Here, we describe the protocol for a HiBiT CETSA, which utilizes a HiBiT tag derived from the NanoLuciferase (NanoLuc) that upo作者: 女上癮 時間: 2025-3-24 15:49
Jian Zhang,Zhiqiang Zhou,Tony T. Lee,Tong Yety and neuronal function. Dysregulation of STEP is linked to the pathophysiology of Alzheimer’s disease and other neuropsychiatric disorders. Experimental results from neurological deficit disease models suggest that the modulation of STEP could be beneficial in a number of these disorders. This pro作者: insipid 時間: 2025-3-24 20:32
https://doi.org/10.1007/978-3-319-69431-3a proteome-wide level. To achieve this, native lysates are first incubated with a compound, followed by a short incubation with a nonspecific protease. Binding of a compound can change accessibility at the binding site or induce other structural changes in the target. This leads to treatment-specifi作者: 山羊 時間: 2025-3-25 01:11
Quantum Fluctuations in Linear Systems,c non-covalent inhibitors, understanding target engagement and selectivity is essential for determining optimal dosing and limiting potential on- or off-target toxicity. Here, we present a complementary activity-based protein profiling (ABPP) strategy for unbiased proteome-wide profiling of cysteine作者: 逃避現(xiàn)實 時間: 2025-3-25 07:21 作者: compassion 時間: 2025-3-25 08:30 作者: EWER 時間: 2025-3-25 14:53 作者: achlorhydria 時間: 2025-3-25 16:17 作者: MUTED 時間: 2025-3-25 21:47 作者: 凈禮 時間: 2025-3-26 01:48 作者: extemporaneous 時間: 2025-3-26 05:03 作者: poliosis 時間: 2025-3-26 12:28
Book 2023ypic assays. The chapters in this book cover topics such as the assembly and use of Kinase Chemogenomics; data mining for chemogenomic compound candidates; protocols for protein family-focused assay systems to profile chemogenomic compounds; functional and target engagement assays in cellular settin作者: Asparagus 時間: 2025-3-26 15:04 作者: motivate 時間: 2025-3-26 16:55 作者: 確定無疑 時間: 2025-3-26 23:38 作者: 通便 時間: 2025-3-27 03:04
Target Deconvolution by Limited Proteolysis Coupled to Mass Spectrometry, identifier PXD035183, this enables the straightforward implementation of the method by scientists with a basic biochemistry and mass spectrometry background. We describe how the procedure can easily be adapted to other protein samples and small molecules.作者: manifestation 時間: 2025-3-27 08:42 作者: 羊齒 時間: 2025-3-27 11:24
Annotation of the Effect of Chemogenomic Compounds on Cell Health Using High-Content Microscopy in rotocol monitors cells during a time course of 48?h using osteosarcoma cells, human embryonic kidney cells, and untransformed human fibroblasts as an example. The described protocol can be easily established and it can be adapted to other cell lines or other parameters important for cellular health.作者: 我沒有命令 時間: 2025-3-27 15:16
Global Assessment of Drug Target Engagement and Selectivity of Covalent Cysteine-Reactive Inhibitor-reactive inhibitors based on two orthogonal approaches. We illustrate the use of clickable alkyne probes for in-gel fluorescence and mass spectrometry studies using a series of therapeutic XPO1 inhibitors as an example.作者: 陶瓷 時間: 2025-3-27 19:03 作者: 眼界 時間: 2025-3-28 00:47 作者: Intervention 時間: 2025-3-28 04:48
https://doi.org/10.1007/978-3-319-66637-2positories to increase target coverage and data accuracy. Here, we present a workflow to generate custom datasets from public databases for mining chemogenomic compound candidates. The compiled set provides flags for differences in structural and bioactivity data and enables rapid extraction of potent and selective bioactive?compounds.作者: 推測 時間: 2025-3-28 07:30 作者: 指令 時間: 2025-3-28 10:31 作者: 暴露他抗議 時間: 2025-3-28 16:09 作者: BRAND 時間: 2025-3-28 21:30
Compilation of Custom Compound/Bioactivity Datasets from Public Repositories,positories to increase target coverage and data accuracy. Here, we present a workflow to generate custom datasets from public databases for mining chemogenomic compound candidates. The compiled set provides flags for differences in structural and bioactivity data and enables rapid extraction of potent and selective bioactive?compounds.作者: 修飾語 時間: 2025-3-29 00:58 作者: 樹上結(jié)蜜糖 時間: 2025-3-29 06:51
Plate-Based Screening for DUB Inhibitors,that control the removal of Ub, are emerging as therapeutic targets. Here, we describe a robust assay suitable for small-molecule inhibitor screening. This assay has the potential to drive the development of small-molecule compounds that can selectively target DUBs.作者: NEG 時間: 2025-3-29 07:29 作者: Strength 時間: 2025-3-29 13:24
https://doi.org/10.1007/978-3-319-66637-2s that drive novel biology. Additionally, the associated data deposited in the public domain have also been used to inform new inhibitor design. Further expansion of this set to complete kinome coverage will allow for a greater understanding of kinase biology and its role in disease.作者: jealousy 時間: 2025-3-29 16:37
Abani K. Pradhan,Abhinav Mishra,Hao Pangheir calculated growth rate to determine a cytotoxic, cytostatic, or healthy outcome. All compounds affecting the growth rate can be further evaluated regarding their specific effects on cell health in a high-content live-cell multiplex assay, described in Chapter ..作者: 比喻好 時間: 2025-3-29 21:58 作者: Countermand 時間: 2025-3-30 00:27
Jian Zhang,Zhiqiang Zhou,Tony T. Lee,Tong Yeel to identify small-molecule STEP inhibitors, we have developed a cellular target engagement assay that can identify compounds that interact with STEP.. We provide a comprehensive protocol to enable the use of this miniaturized assay, and we demonstrate its utility to benchmark the binding of newly discovered compounds.作者: Irrepressible 時間: 2025-3-30 07:53 作者: 叫喊 時間: 2025-3-30 08:16 作者: Subdue 時間: 2025-3-30 13:19
Developing a Kinase Chemogenomic Set: Facilitating Investigation into Kinase Biology by Linking Phes that drive novel biology. Additionally, the associated data deposited in the public domain have also been used to inform new inhibitor design. Further expansion of this set to complete kinome coverage will allow for a greater understanding of kinase biology and its role in disease.作者: GONG 時間: 2025-3-30 18:08 作者: 難聽的聲音 時間: 2025-3-30 23:05 作者: 過于平凡 時間: 2025-3-31 02:49
Detection of Cellular Target Engagement for Small-Molecule Modulators of Striatal-Enriched Protein el to identify small-molecule STEP inhibitors, we have developed a cellular target engagement assay that can identify compounds that interact with STEP.. We provide a comprehensive protocol to enable the use of this miniaturized assay, and we demonstrate its utility to benchmark the binding of newly discovered compounds.作者: 去才蔑視 時間: 2025-3-31 06:57 作者: Confound 時間: 2025-3-31 10:21
