標(biāo)題: Titlebook: Chemical Genomics; Reviews and Protocol Edward D. Zanders Book 2005 Humana Press 2005 Escherichia coli.Microarray.Proteomics.genetic inform [打印本頁] 作者: 人工合成 時(shí)間: 2025-3-21 19:04
書目名稱Chemical Genomics影響因子(影響力)
書目名稱Chemical Genomics影響因子(影響力)學(xué)科排名
書目名稱Chemical Genomics網(wǎng)絡(luò)公開度
書目名稱Chemical Genomics網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Chemical Genomics被引頻次
書目名稱Chemical Genomics被引頻次學(xué)科排名
書目名稱Chemical Genomics年度引用
書目名稱Chemical Genomics年度引用學(xué)科排名
書目名稱Chemical Genomics讀者反饋
書目名稱Chemical Genomics讀者反饋學(xué)科排名
作者: abolish 時(shí)間: 2025-3-21 23:37
Computer-Aided Design of Small Molecules for Chemical Genomicsor creating molecules for chemical genomics. The design process involves manipulation of the input, modification of structural constraints, and further processing of the .-generated molecules using various modular toolkits. The development of a theoretical framework for each of these stages will pro作者: Wordlist 時(shí)間: 2025-3-22 01:39
Design, Synthesis, and Screening of Biomimetic Ligands for Affinity Chromatographye affinity ligands that represent a promising class of synthetic affinity ligands have been developed to target specific proteins and designed to mimic peptidal templates, natural biological recognition motifs, or complementary surface-exposed residues. These biomimetic ligands have been generated b作者: 軌道 時(shí)間: 2025-3-22 07:22
The Role and Application of , Docking in Chemical Genomics Researches can be used to identify the structural features that are important for binding and for . screening efforts in which suitable binding partners can be identified. Here we describe a practical approach for setting up docking simulations using different docking programs. We also cover the analysis an作者: Mindfulness 時(shí)間: 2025-3-22 08:57
Synthesis of Complex Carbohydrates and Glyconjugatesex5 glycoform. Genome analysis identified an open reading frame, HI1578, referred to as LgtD, whose amino acid sequence shows a significant level of similarity to those of a number of bacterial glycosyltransferases involved in lipopolysaccharide biosynthesis. To investigate its function, overexpress作者: Nonporous 時(shí)間: 2025-3-22 14:55 作者: Nonporous 時(shí)間: 2025-3-22 21:01
Screening for the Expression of Soluble Recombinant Protein in , in most proteomics pipelines. . (.) is the most convenient and cost-effective host, although optimal conditions for the expression of different proteins vary widely. Proteins vary in their structural stability, solubility, and toxicity in this environment, resulting in differing rates of protein de作者: Subdue 時(shí)間: 2025-3-22 22:11 作者: 案發(fā)地點(diǎn) 時(shí)間: 2025-3-23 03:00 作者: tinnitus 時(shí)間: 2025-3-23 08:05
Production of Proteins for NMR Studies Using the Wheat Germ Cell-Free Systemerm cell-free system (without purification). A comparison of .H-.N heteronuclear single-quantum coherence (HSQC) spectra of yeast ubiquitin prepared using each method indicates that this wheat germ cell-free system may be used for rapid nuclear magnetic resonance analyses of proteins without purific作者: HERTZ 時(shí)間: 2025-3-23 13:21
Fabrication of Protein Function Microarrays for Systems-Oriented Proteomic Analysiss for array fabrication are required. Here we discuss the background to different routes to array fabrication and describe in detail one approach in which the purification and immobilization procedures are combined into a single step, dramatically simplifying the array fabrication process. We illust作者: Synchronism 時(shí)間: 2025-3-23 16:59
Peptide and Small-Molecule Microarraysthe functionalization of a glass slide with a glyoxylyl group, followed by chemoselective ligation of small molecules or peptides to the functionalized surface via a covalent bond. In the second method, peptides or small molecules are first conjugated to a macromolecular scaffold. The final ligand-s作者: 預(yù)感 時(shí)間: 2025-3-23 18:21 作者: 官僚統(tǒng)治 時(shí)間: 2025-3-23 22:40 作者: FID 時(shí)間: 2025-3-24 05:43
Production of Proteins for NMR Studies Using the Wheat Germ Cell-Free Systemerm cell-free system (without purification). A comparison of .H-.N heteronuclear single-quantum coherence (HSQC) spectra of yeast ubiquitin prepared using each method indicates that this wheat germ cell-free system may be used for rapid nuclear magnetic resonance analyses of proteins without purification.作者: 無法解釋 時(shí)間: 2025-3-24 09:46
https://doi.org/10.1007/978-3-7091-4087-1d organisms by selective small-molecule ligands or modulators. In this overview, we are focusing on diversity aspects, design methods, and chemical synthesis strategies for the application of small molecules as tools for chemical genomics. Examples for different successful chemical-genomics strategi作者: 冷淡一切 時(shí)間: 2025-3-24 10:58
https://doi.org/10.1007/978-3-7091-4087-1or creating molecules for chemical genomics. The design process involves manipulation of the input, modification of structural constraints, and further processing of the .-generated molecules using various modular toolkits. The development of a theoretical framework for each of these stages will pro作者: paroxysm 時(shí)間: 2025-3-24 17:59 作者: 天真 時(shí)間: 2025-3-24 19:12
,Wettbewerb Kohle — Wasserkraft,es can be used to identify the structural features that are important for binding and for . screening efforts in which suitable binding partners can be identified. Here we describe a practical approach for setting up docking simulations using different docking programs. We also cover the analysis an作者: GROUP 時(shí)間: 2025-3-25 02:00
Beileitung und Pumpspeicherung,ex5 glycoform. Genome analysis identified an open reading frame, HI1578, referred to as LgtD, whose amino acid sequence shows a significant level of similarity to those of a number of bacterial glycosyltransferases involved in lipopolysaccharide biosynthesis. To investigate its function, overexpress作者: gruelling 時(shí)間: 2025-3-25 06:49 作者: LEVER 時(shí)間: 2025-3-25 07:46 作者: ciliary-body 時(shí)間: 2025-3-25 13:00 作者: 間諜活動(dòng) 時(shí)間: 2025-3-25 18:04 作者: Fibrin 時(shí)間: 2025-3-25 23:42
,Wettbewerb Kohle — Wasserkraft,erm cell-free system (without purification). A comparison of .H-.N heteronuclear single-quantum coherence (HSQC) spectra of yeast ubiquitin prepared using each method indicates that this wheat germ cell-free system may be used for rapid nuclear magnetic resonance analyses of proteins without purific作者: Affiliation 時(shí)間: 2025-3-26 00:47
,Wettbewerb Kohle — Wasserkraft,s for array fabrication are required. Here we discuss the background to different routes to array fabrication and describe in detail one approach in which the purification and immobilization procedures are combined into a single step, dramatically simplifying the array fabrication process. We illust作者: Outwit 時(shí)間: 2025-3-26 07:33 作者: 食物 時(shí)間: 2025-3-26 12:33
https://doi.org/10.1007/978-3-7091-4087-1id and sensitive identification of proteins by peptide mass fingerprinting (PMF). We describe a simple protocol that can be performed in a standard biochemistry laboratory, whereby proteins separated by one- or two-dimensional gel electrophoresis can be identified at femtomole levels. The procedure 作者: 審問 時(shí)間: 2025-3-26 15:46
Bogengangsapparat und Nystagmus,molecules of diverse structures, including polysaccharides, natural glycoconjugates, and mono- and oligosaccharides coupled to carrier molecules, can be stably immobilized on a glass chip without chemical modification. Here, we describe a practical protocol for this technology. We hope that anyone w作者: temperate 時(shí)間: 2025-3-26 18:49
Edward D. ZandersIncludes supplementary material: 作者: Agility 時(shí)間: 2025-3-26 21:30
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/224341.jpg作者: Initiative 時(shí)間: 2025-3-27 02:48
https://doi.org/10.1007/978-3-7091-4087-1Small-molecule drugs are a cost-effective way to treat and prevent disease. A study by the Slone Institute published in 2002 estimated that over 50% of the adult population in the United States used at least one pharmaceutical drug during the preceding week. The positive impact of small-molecule drugs on health care has been well documented (.,.).作者: 不朽中國 時(shí)間: 2025-3-27 06:20
,Wettbewerb Kohle — Wasserkraft,This chapter contains protocols for high-throughput protein production based on the cell-free system prepared from eukaryote wheat embryos.作者: 分期付款 時(shí)間: 2025-3-27 09:51 作者: 卵石 時(shí)間: 2025-3-27 17:16
Introduction to Chemical GenomicsSmall-molecule drugs are a cost-effective way to treat and prevent disease. A study by the Slone Institute published in 2002 estimated that over 50% of the adult population in the United States used at least one pharmaceutical drug during the preceding week. The positive impact of small-molecule drugs on health care has been well documented (.,.).作者: 固定某物 時(shí)間: 2025-3-27 20:24
The Wheat Germ Cell-Free Expression SystemThis chapter contains protocols for high-throughput protein production based on the cell-free system prepared from eukaryote wheat embryos.作者: 彈藥 時(shí)間: 2025-3-27 22:05 作者: Leisureliness 時(shí)間: 2025-3-28 05:34 作者: Cocker 時(shí)間: 2025-3-28 08:04 作者: 注視 時(shí)間: 2025-3-28 11:19
https://doi.org/10.1007/978-1-59259-948-6Escherichia coli; Microarray; Proteomics; genetic information; molecular biology; organic chemistry; prote作者: 啪心兒跳動(dòng) 時(shí)間: 2025-3-28 17:45
Beileitung und Pumpspeicherung,e assay, high-performance liquid chromatography (HPLC), and liquid chromatography (LC)/mass spectrometry (MS) show that LgtD is an .-acetylgalactosaminyltransferase with high donor substrate specificity, and globotriose is a highly preferred acceptor substrate for the enzyme.作者: 毛細(xì)血管 時(shí)間: 2025-3-28 20:42 作者: Alveolar-Bone 時(shí)間: 2025-3-29 00:15 作者: 北極人 時(shí)間: 2025-3-29 03:50
A Practical Protocol for Carbohydrate Microarraysexample to illustrate that the carbohydrate microarray is also a discovery tool; this is particularly useful for identifying immunologic sugar moieties, including complex carbohydrates of cancer cells and sugar signatures of previously unrecognized microbial pathogens.作者: tendinitis 時(shí)間: 2025-3-29 09:29
,Wettbewerb Kohle — Wasserkraft,y a combination of rational design, combinatorial library synthesis, and subsequent screening of potential leads against target proteins. Small ligands based on a triazine scaffold also present exceptional selectivity and stability, which allows their use in harsh manufacturing environments.作者: 粘土 時(shí)間: 2025-3-29 11:49
https://doi.org/10.1007/978-3-7091-4087-1 any vector LIC-enabled. It also includes a protocol for generating high-quality linearized vector template for the LIC reaction. Lastly, a step-by-step protocol of the LIC reaction is outlined, with useful tips and tricks for optimization and screening.作者: 神圣在玷污 時(shí)間: 2025-3-29 19:25
https://doi.org/10.1007/978-3-7091-4087-1involves excision of the spot or band from the gel, washing and de-staining, reduction and alkylation, in-gel trypsin digestion, MALDI-TOF MS of the tryptic peptides, and database searching of the PMF data. Up to 96 protein samples can easily be manually processed at one time by this method.作者: 清真寺 時(shí)間: 2025-3-29 22:23 作者: Peak-Bone-Mass 時(shí)間: 2025-3-30 01:48 作者: 無聊點(diǎn)好 時(shí)間: 2025-3-30 04:50 作者: deviate 時(shí)間: 2025-3-30 09:01
,Wettbewerb Kohle — Wasserkraft,hich the purification and immobilization procedures are combined into a single step, dramatically simplifying the array fabrication process. We illustrate this approach by reference to the creation of an array of p53 variants, and discuss methods for assay and data analysis on such arrays.作者: 步履蹣跚 時(shí)間: 2025-3-30 12:30
Beileitung und Pumpspeicherung,d surface via a covalent bond. In the second method, peptides or small molecules are first conjugated to a macromolecular scaffold. The final ligand-scaffold conjugates are then spotted and adsorbed onto the solid surface. Three different assay methods to screen such chemical microarrays are described.作者: apiary 時(shí)間: 2025-3-30 20:15
Chemistry for Chemical Genomicsnthesis strategies for the application of small molecules as tools for chemical genomics. Examples for different successful chemical-genomics strategies include the selection of diverse drug-like molecules, target family—focused compound libraries, natural-product chemistry, and diversity-oriented synthesis.作者: Magnificent 時(shí)間: 2025-3-30 22:27 作者: exacerbate 時(shí)間: 2025-3-31 01:42
Fabrication of Protein Function Microarrays for Systems-Oriented Proteomic Analysishich the purification and immobilization procedures are combined into a single step, dramatically simplifying the array fabrication process. We illustrate this approach by reference to the creation of an array of p53 variants, and discuss methods for assay and data analysis on such arrays.作者: 內(nèi)閣 時(shí)間: 2025-3-31 06:28
Peptide and Small-Molecule Microarraysd surface via a covalent bond. In the second method, peptides or small molecules are first conjugated to a macromolecular scaffold. The final ligand-scaffold conjugates are then spotted and adsorbed onto the solid surface. Three different assay methods to screen such chemical microarrays are described.作者: 發(fā)誓放棄 時(shí)間: 2025-3-31 13:11
