標(biāo)題: Titlebook: Chemical Biology; Methods and Protocol Jonathan E. Hempel,Charles H. Williams,Charles C. Book 2015 Springer Science+Business Media New Yor [打印本頁] 作者: 營養(yǎng)品 時(shí)間: 2025-3-21 16:51
書目名稱Chemical Biology影響因子(影響力)
書目名稱Chemical Biology影響因子(影響力)學(xué)科排名
書目名稱Chemical Biology網(wǎng)絡(luò)公開度
書目名稱Chemical Biology網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Chemical Biology被引頻次
書目名稱Chemical Biology被引頻次學(xué)科排名
書目名稱Chemical Biology年度引用
書目名稱Chemical Biology年度引用學(xué)科排名
書目名稱Chemical Biology讀者反饋
書目名稱Chemical Biology讀者反饋學(xué)科排名
作者: peritonitis 時(shí)間: 2025-3-21 21:43
1064-3745 , .Chemical Biology: Methods and Protocols. seeks to improve the success rate of the chemical biology field through the dissemination of detailed and experiential knowledge..978-1-4939-5284-7978-1-4939-2269-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: apiary 時(shí)間: 2025-3-22 01:13
Applying the Logic of Genetic Interaction to Discover Small Molecules That Functionally Interact withat differ the most across the two classes). A technique called compound set enrichment analysis (CSEA), modeled after a widely used method to identify pathways from gene expression data, identifies sets of functionally or structurally related compounds that are statistically enriched among the most作者: doxazosin 時(shí)間: 2025-3-22 07:01 作者: 訓(xùn)誡 時(shí)間: 2025-3-22 09:15
Dependency Properties and Routed Events, is hampered by resistance. New drug development, like routine screening for drug resistance, has however been hampered by the lack of reliable screening protocols with sufficient throughput. Here we report on two separate in vitro protocols that use fluorescent dyes and allow for standardized drug sensitivity testing on the required scale.作者: charisma 時(shí)間: 2025-3-22 16:13 作者: charisma 時(shí)間: 2025-3-22 20:20 作者: Vulnerable 時(shí)間: 2025-3-22 23:56
https://doi.org/10.1007/978-1-4302-1603-2ssay-guided isolation is a rapid and validated method for isolation of compounds with bioactivities. This chapter describes bioassay-guided separation and purification of compounds from the crude extracts of TCMs. Two approaches including size-exclusion chromatography (SEC) and high performance liquid chromatography (HPLC) are described in detail.作者: Gesture 時(shí)間: 2025-3-23 05:02 作者: paragon 時(shí)間: 2025-3-23 07:56
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/224284.jpg作者: Type-1-Diabetes 時(shí)間: 2025-3-23 10:04 作者: Connotation 時(shí)間: 2025-3-23 17:51
Styles, Templets, and Custom Controls, to clinical translation. Here, we present a chemical biology approach that uses perturbation with small molecules of known mechanism to reveal mechanistic and therapeutic consequences of human disease alleles. To maximize human applicability, we perform chemical screening on multiple cell lines iso作者: Oligarchy 時(shí)間: 2025-3-23 20:50
Styles, Templates, and Custom Controls,tion of small-molecule compounds spotted and immobilized on a glass slide surface, providing a multiplex platform to identify small-molecule compounds binding to a protein of interest in high-throughput screening. Several research groups have developed a variety of methods for the immobilization of 作者: APO 時(shí)間: 2025-3-24 02:16 作者: Malleable 時(shí)間: 2025-3-24 03:07 作者: MANIA 時(shí)間: 2025-3-24 06:39 作者: ORE 時(shí)間: 2025-3-24 14:01 作者: BILE 時(shí)間: 2025-3-24 16:32 作者: 易于 時(shí)間: 2025-3-24 20:51 作者: Ligament 時(shí)間: 2025-3-25 00:56
Dependency Properties and Routed Events,and humans share many structurally and functionally related gene families. Development of such disease models in the fly allows genetic approaches to be applied to address specific hypotheses concerning disease progression and to test candidate modifier genes. More recently these fly models have als作者: Aesthete 時(shí)間: 2025-3-25 06:39
Dependency Properties and Routed Events, phenomena, as they allow modulation of enzymatic activities and temporal control of protein action. Here, we describe the methodology to conduct large-scale screens for chemical compounds that induce a desired phenotype in the roundworm . (.) using 96- or 384-well microtiter plates.作者: 移動(dòng) 時(shí)間: 2025-3-25 10:07 作者: fixed-joint 時(shí)間: 2025-3-25 14:32 作者: MARS 時(shí)間: 2025-3-25 17:29 作者: 滴注 時(shí)間: 2025-3-25 21:45
https://doi.org/10.1007/978-1-4302-1603-2ssay-guided isolation is a rapid and validated method for isolation of compounds with bioactivities. This chapter describes bioassay-guided separation and purification of compounds from the crude extracts of TCMs. Two approaches including size-exclusion chromatography (SEC) and high performance liqu作者: transient-pain 時(shí)間: 2025-3-26 02:39
Dependency Properties and Routed Events,fragment hits have lower molecular weight (<300–350 Da) and lower initial potency but higher ligand efficiency when compared to those from high-throughput screening. NMR spectroscopy has been widely used for FBDD since it identifies and localizes the binding site of weakly interacting hits on the ta作者: 撕裂皮肉 時(shí)間: 2025-3-26 07:34 作者: 光亮 時(shí)間: 2025-3-26 12:29 作者: ALERT 時(shí)間: 2025-3-26 14:44
Whole-Organism Screening for Modulators of Fasting Metabolism Using Transgenic Zebrafishnging using traditional high-throughput approaches in vitro. Here, we describe a method that rapidly identifies small molecules with an impact on organismal energy balance in vivo. Specifically, we developed a whole-organism screen for modulators of fasting metabolism using transgenic bioluminescence-reporter zebrafish for the gluconeogenic gene .作者: reserve 時(shí)間: 2025-3-26 19:51
Bioassay-Guided Identification of Bioactive Molecules from Traditional Chinese Medicinesssay-guided isolation is a rapid and validated method for isolation of compounds with bioactivities. This chapter describes bioassay-guided separation and purification of compounds from the crude extracts of TCMs. Two approaches including size-exclusion chromatography (SEC) and high performance liquid chromatography (HPLC) are described in detail.作者: 通便 時(shí)間: 2025-3-26 20:59
https://doi.org/10.1007/978-1-4939-2269-7biological function; chemical; functional protein targets; in vitro cellular systems; in vitro-based com作者: 系列 時(shí)間: 2025-3-27 04:57
978-1-4939-5284-7Springer Science+Business Media New York 2015作者: 疏遠(yuǎn)天際 時(shí)間: 2025-3-27 08:54 作者: 身心疲憊 時(shí)間: 2025-3-27 11:56
1064-3745 ation advice from the experts.Includes supplementary materia.This volume seeks to enable the discovery of tools in chemical biology by providing readers with various techniques ranging from initial chemical genetic screening to target identification. To successfully highlight the essential component作者: 果核 時(shí)間: 2025-3-27 15:45
https://doi.org/10.1007/978-1-4302-1603-2ulates a legion of complex chemical reactions found in intact cells. Specifically, we focus on the use of a luciferase-based fusion system to identify small-molecule modulators that affect protein turnover.作者: Mhc-Molecule 時(shí)間: 2025-3-27 18:40 作者: Tidious 時(shí)間: 2025-3-27 23:59
https://doi.org/10.1007/978-1-4302-1603-2eening. Here, we describe a simple method for CLS measurement using aging minicultures in microtiter plates and batch plate-back for the determination of culture viability. This assay can be used to screen a large number of strains, conditions, or compounds in parallel for effects on aging.作者: 有機(jī)體 時(shí)間: 2025-3-28 03:50
Dependency Properties and Routed Events,hput screening. NMR spectroscopy has been widely used for FBDD since it identifies and localizes the binding site of weakly interacting hits on the target protein. Here we describe ligand-based NMR methods for hit identification from fragment libraries and for functional cross-validation of primary hits.作者: commute 時(shí)間: 2025-3-28 10:01
Small-Molecule High-Throughput Screening Utilizing , Egg Extractulates a legion of complex chemical reactions found in intact cells. Specifically, we focus on the use of a luciferase-based fusion system to identify small-molecule modulators that affect protein turnover.作者: Galactogogue 時(shí)間: 2025-3-28 10:24 作者: foliage 時(shí)間: 2025-3-28 15:36 作者: 貞潔 時(shí)間: 2025-3-28 21:46
NMR Screening in Fragment-Based Drug Design: A Practical Guidehput screening. NMR spectroscopy has been widely used for FBDD since it identifies and localizes the binding site of weakly interacting hits on the target protein. Here we describe ligand-based NMR methods for hit identification from fragment libraries and for functional cross-validation of primary hits.作者: 疾馳 時(shí)間: 2025-3-29 00:15 作者: 針葉樹 時(shí)間: 2025-3-29 04:23 作者: Pert敏捷 時(shí)間: 2025-3-29 08:58
Dependency Properties and Routed Events,o been used for drug discovery. Here, we describe how to utilize the existing fruit fly models of human neurological disorders to identify small-molecule leads that could potentially be further developed for therapeutic use.作者: 運(yùn)動(dòng)吧 時(shí)間: 2025-3-29 11:38 作者: meretricious 時(shí)間: 2025-3-29 19:01
Book 2015etic screening to target identification. To successfully highlight the essential components of the chemical biology tool discovery process, the book is organizes into four parts that focus on platforms for molecular discovery in .in vitro .cellular systems, .in vivo. chemical genetic screening proto作者: endoscopy 時(shí)間: 2025-3-29 22:54 作者: Cytokines 時(shí)間: 2025-3-30 03:20 作者: MAOIS 時(shí)間: 2025-3-30 06:36
Small-Molecule Screening Using , Models of Human Neurological Disorderso been used for drug discovery. Here, we describe how to utilize the existing fruit fly models of human neurological disorders to identify small-molecule leads that could potentially be further developed for therapeutic use.作者: faultfinder 時(shí)間: 2025-3-30 09:52 作者: Explicate 時(shí)間: 2025-3-30 14:55 作者: Assignment 時(shí)間: 2025-3-30 16:49
https://doi.org/10.1007/978-1-4302-1603-2ere, we describe methods used to develop high content screening assays implementing multipotent cardiovascular progenitors derived from human pluripotent stem cells and have identified novel compounds that direct cardiac differentiation.作者: 皮薩 時(shí)間: 2025-3-30 22:45 作者: expeditious 時(shí)間: 2025-3-31 02:08 作者: elucidate 時(shí)間: 2025-3-31 07:58
High Content Screening for Modulators of Cardiac Differentiation in Human Pluripotent Stem Cellsere, we describe methods used to develop high content screening assays implementing multipotent cardiovascular progenitors derived from human pluripotent stem cells and have identified novel compounds that direct cardiac differentiation.作者: Genteel 時(shí)間: 2025-3-31 12:21
High Content Screening for Modulators of Cardiovascular or Global Developmental Pathways in Zebrafisssays, chemically targeting select pathways without causing pleiotropic effects. Here, we describe methods used to develop high content screening assays implementing transgenic zebrafish to assess phenotypic changes that have identified several classes of novel compounds that effect developmental pathways.作者: 過份艷麗 時(shí)間: 2025-3-31 15:25
Identification of Therapeutic Small-Molecule Leads in Cultured Cells Using Multiplexed Pathway Reporegies to single out reagents with sought-after cellular activities. In addition to representing high-priority therapeutic targets, intensely studied cell signaling systems encapsulate robust reference points for mapping novel chemical activities given our deep understanding of the molecular mechanis作者: triptans 時(shí)間: 2025-3-31 18:02 作者: 充足 時(shí)間: 2025-3-31 22:23
