標(biāo)題: Titlebook: Characterization of Nanoparticles Intended for Drug Delivery; Jeffrey D. Clogston,Rachael M. Crist,Stephan T. St Book 2024Latest edition L [打印本頁] 作者: antibody 時間: 2025-3-21 17:06
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作者: Gourmet 時間: 2025-3-21 21:54 作者: ERUPT 時間: 2025-3-22 02:31 作者: HAUNT 時間: 2025-3-22 05:28
Assessment of Protein Binding Using Asymmetric-Flow Field-Flow Fractionation Combined with Multi-ange nanoparticle and suggests the presence of protein binding. This protocol will discuss how to set up an experiment to assess protein binding in nanoparticles using AF4, multi-angle light scattering (MALS), and dynamic light scattering (DLS).作者: 銼屑 時間: 2025-3-22 10:20 作者: MEET 時間: 2025-3-22 16:51
Particle Size and Concentration Measurement Using the Spectradyne nCS1 Instrumentnano-constriction in the microfluidic cartridge. This method also has the advantage over optical techniques in that measurements are not dependent on the type of material being measured (e.g., refractive index of the sample itself is not needed for accurate analysis), and only microliters (typically 5?μL) of a sample are needed for analysis.作者: MEET 時間: 2025-3-22 19:41 作者: Gentry 時間: 2025-3-22 22:17 作者: 索賠 時間: 2025-3-23 03:30
Detection of Nanoparticle-Mediated Total Oxidative Stress in T Cells Using CM-H2DCFDA Dye T cells isolated from the peripheral blood of healthy donor volunteers are treated with nanoparticles and controls, and the generation of reactive oxygen species is detected by flow cytometry using CM-H.DCFDA reagent.作者: SOB 時間: 2025-3-23 07:28
Detection of Induction of Mitochondrial Oxidative Stress by Nanoparticles in T Cells Using MitoSOX Rogy but in other areas of biology as well, including those related to developing novel therapies. Total oxidative stress may result from multiple cellular organelles. The protocol described herein allows for the analysis of oxidative stress in mitochondria.作者: 社團(tuán) 時間: 2025-3-23 12:32
Detection of Nanoparticle-Mediated Change in Mitochondrial Membrane Potential in T Cells Using JC-1 therapeutic applications. The protocol described herein utilizes JC-1 dye to detect nanoparticle-mediated changes in mitochondrial membrane potential and is intended to support mechanistic immunotoxicology studies.作者: 強(qiáng)制性 時間: 2025-3-23 15:50
1064-3745 ation advice from the expertsThis third edition volume expands on the previous editions with new and updated discussions on the latest developments in endotoxin contamination, complex physicochemical properties, in vitro immunotoxicity traits, and in vitro drug release properties. Eight chapters in 作者: 我要沮喪 時間: 2025-3-23 18:34 作者: CHYME 時間: 2025-3-23 23:23 作者: 不能強(qiáng)迫我 時間: 2025-3-24 06:00
Therapie gastrointestinaler Erkrankungennano-constriction in the microfluidic cartridge. This method also has the advantage over optical techniques in that measurements are not dependent on the type of material being measured (e.g., refractive index of the sample itself is not needed for accurate analysis), and only microliters (typically 5?μL) of a sample are needed for analysis.作者: enfeeble 時間: 2025-3-24 08:15
https://doi.org/10.1007/978-3-662-09397-9th high resolution, thus allowing the measurement of multiple particle populations for the quality assessment of nanoformulations. Additionally, spICP-MS can accurately determine particle concentrations without the need for concentration standards.作者: vitreous-humor 時間: 2025-3-24 13:00
,Zellz?hl- und Differenzierger?te,technology platforms possess intrinsic anti-inflammatory properties and may benefit the therapy of inflammation-driven disorders. Herein, we present a protocol to study nanotechnology concepts’ anti-inflammatory properties in a chemically-induced psoriasis model.作者: Little 時間: 2025-3-24 18:42
https://doi.org/10.1007/978-3-642-74928-5 T cells isolated from the peripheral blood of healthy donor volunteers are treated with nanoparticles and controls, and the generation of reactive oxygen species is detected by flow cytometry using CM-H.DCFDA reagent.作者: Bouquet 時間: 2025-3-24 21:55
Praktische Energiebedarfsforschungogy but in other areas of biology as well, including those related to developing novel therapies. Total oxidative stress may result from multiple cellular organelles. The protocol described herein allows for the analysis of oxidative stress in mitochondria.作者: Morsel 時間: 2025-3-25 02:24 作者: epicondylitis 時間: 2025-3-25 03:20 作者: 漂泊 時間: 2025-3-25 09:55
Ion Quantitation in Liposomal Products Using RP-HPLC with Charged Aerosol Detectionmmonium and sulfate ions in liposomal products, using generic PEGylated liposomal doxorubicin as an example. This method can be extended to calcium, acetate, and other ions in different liposomal formulations with slight modifications.作者: Enthralling 時間: 2025-3-25 13:52
https://doi.org/10.1007/978-1-0716-3786-9Nanomedicine; Physicochemical characterization; Immunotoxicity; Nanomaterials; Pharmaceuticals作者: SOBER 時間: 2025-3-25 19:09 作者: BLAZE 時間: 2025-3-25 21:53
Jeffrey D. Clogston,Rachael M. Crist,Stephan T. StIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 宣傳 時間: 2025-3-26 02:53 作者: 從屬 時間: 2025-3-26 04:17
https://doi.org/10.1007/978-3-662-36382-9 ideal nor thoroughly descriptive enough to define the full complexity of these materials. Asymmetric-flow field-flow fractionation (AF4) with various in-line detectors, such as ultraviolet–visible (UV–vis), multi-angle light scattering (MALS), refractive index (RI), and DLS, is an alternative techn作者: 低三下四之人 時間: 2025-3-26 11:12
https://doi.org/10.1007/978-3-662-36382-9with both static light scattering and dynamic light scattering, it can be used to qualitatively assess protein binding to nanoparticles by comparing the shape factors for both non-plasma-incubated samples and plasma-incubated samples. The shape factor is defined as the ratio of the derived root mean作者: Harass 時間: 2025-3-26 14:49
https://doi.org/10.1007/978-3-662-36382-9e pharmaceutical ingredients (API). Since the amount of the API in a polymeric prodrug product directly impacts both safety and efficacy, there is a pressing need for robust and accurate analytical methods to quantify the API in these formulations. Presently, drug quantification methods include reve作者: 增減字母法 時間: 2025-3-26 18:42
Therapie gastrointestinaler Erkrankungenadyne nCS1 is a particle-analyzing instrument that uses microfluidic resistive pulse sensing, rather than optical measurements, to determine the size and concentration of samples. The size and concentration of a sample are determined by measuring the changes in voltage as particles travel through a 作者: 混亂生活 時間: 2025-3-26 23:29
https://doi.org/10.1007/978-3-662-09397-9try (ICP-MS) in single-particle (sp) mode. Accurately determining the size of individual nanoparticles on a per-particle basis, both quickly and accurately, is an ever-increasing need within nanoparticle characterization. ICP-MS is capable of measuring a broad range of metallic nanoparticle sizes wi作者: 不真 時間: 2025-3-27 03:11
Therapie endokrinologischer Erkrankungengs is challenging due to the absence of chromophores or fluorophores of ions and the efficiency of their release from the liposome structure. To address these issues, a method based on reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with a charged aerosol detector (CAD) has b作者: NAVEN 時間: 2025-3-27 06:14 作者: Synovial-Fluid 時間: 2025-3-27 10:40 作者: Insatiable 時間: 2025-3-27 15:53
https://doi.org/10.1007/978-3-662-09398-6ucans are present in yeast and fungi, and, for this reason, their traces are commonly used as a sign of yeast or fungal infection or contamination. Despite being less immunologically active than endotoxins, beta-glucans are pro-inflammatory and can activate cytokines and other immunological response作者: Autobiography 時間: 2025-3-27 20:59 作者: 廢止 時間: 2025-3-28 00:28
H.-P. Lipp,H. Hebart,C. Faul,H. Einselens is DNA; therefore, screening for antibodies recognizing single- and/or double-stranded DNA is commonly used to detect and classify autoimmune diseases. While autoimmunity affects both sexes, females are generally more affected than males, which is recapitulated in some animal models. A variety of作者: Mortal 時間: 2025-3-28 03:58 作者: 取之不竭 時間: 2025-3-28 10:09
https://doi.org/10.1007/978-3-642-74928-5 (e.g., anti-inflammatory and tumor growth inhibiting) responses have been linked in the literature to oxidative stress, emphasizing the importance of developing methodologies to study this phenomenon in cells following their exposure to nanoparticles. In the protocol described herein, primary human作者: 鋪?zhàn)?nbsp; 時間: 2025-3-28 14:25
Praktische Energiebedarfsforschungd tissues. In contrast, when directed against cancer and autoinflammatory diseases, some nanomaterials inducing oxidative stress have also been reported as potential therapies for these disorders. Therefore, studying oxidative stress has become a popular tool not only in toxicology and immunotoxicol作者: 恫嚇 時間: 2025-3-28 16:07 作者: Harness 時間: 2025-3-28 21:55
Characterization of Nanoparticles Intended for Drug Delivery978-1-0716-3786-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 最低點(diǎn) 時間: 2025-3-28 23:10
Nanoparticle Size Distribution and Stability Assessment Using Asymmetric-Flow Field-Flow Fractionati ideal nor thoroughly descriptive enough to define the full complexity of these materials. Asymmetric-flow field-flow fractionation (AF4) with various in-line detectors, such as ultraviolet–visible (UV–vis), multi-angle light scattering (MALS), refractive index (RI), and DLS, is an alternative techn作者: 善于騙人 時間: 2025-3-29 03:18 作者: 溝通 時間: 2025-3-29 09:17 作者: 最后一個 時間: 2025-3-29 12:11 作者: eczema 時間: 2025-3-29 16:08
Measuring Size and Number Concentration of Metallic Nanoparticles Using spICP-MStry (ICP-MS) in single-particle (sp) mode. Accurately determining the size of individual nanoparticles on a per-particle basis, both quickly and accurately, is an ever-increasing need within nanoparticle characterization. ICP-MS is capable of measuring a broad range of metallic nanoparticle sizes wi作者: accrete 時間: 2025-3-29 20:39
Ion Quantitation in Liposomal Products Using RP-HPLC with Charged Aerosol Detectiongs is challenging due to the absence of chromophores or fluorophores of ions and the efficiency of their release from the liposome structure. To address these issues, a method based on reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with a charged aerosol detector (CAD) has b作者: 輕快帶來危險 時間: 2025-3-30 01:07 作者: Traumatic-Grief 時間: 2025-3-30 05:33
Current Considerations and Practical Solutions for Overcoming Nanoparticle Interference with LAL Assproducts. Endotoxin contamination of engineered nanomaterials and nanotechnology-based medical products represents a significant translational hurdle. Nanoparticles often interfere with an in vitro limulus amebocyte lysate (LAL) assay commonly used in the pharmaceutical industry for the detection an作者: 吝嗇性 時間: 2025-3-30 12:10
Detection of Beta-Glucan Contamination in Nanoparticle Formulationsucans are present in yeast and fungi, and, for this reason, their traces are commonly used as a sign of yeast or fungal infection or contamination. Despite being less immunologically active than endotoxins, beta-glucans are pro-inflammatory and can activate cytokines and other immunological response作者: 廚師 時間: 2025-3-30 14:06
Detection of Intracellular Complement Activation by Nanoparticles in Human T Lymphocytesplosome. The complement proteins expressed by the liver and secreted into blood plasma compose the plasma complement system, whereas complement proteins expressed by and functioning inside the cell represent the intracellular complement. The complement system plays an essential role in host defense;作者: rectocele 時間: 2025-3-30 17:29 作者: Assault 時間: 2025-3-31 00:47
Analysis of Nanoparticles’ Effects on Drug-Induced Psoriasis the treatments include anti-inflammatory medications. Nanoparticles are widely used for drug delivery and have found successful applications in therapy for cancer and infectious diseases. Nanoparticles can also be used to deliver anti-inflammatory drugs to sites of inflammation. Moreover, some nano作者: Ventilator 時間: 2025-3-31 01:49 作者: Eviction 時間: 2025-3-31 08:06 作者: 廣口瓶 時間: 2025-3-31 10:54
Detection of Nanoparticle-Mediated Change in Mitochondrial Membrane Potential in T Cells Using JC-1 cells is beneficial for cancer therapy, cytotoxicity to healthy cells may limit the therapeutic applications of mitochondria-damaging nanoparticles. Due to the critical role mitochondria play in cell viability and function, it is important to detect such alterations when studying nanomaterials for 作者: 磨坊 時間: 2025-3-31 13:33
Characterization of Nanoparticles Intended for Drug Delivery作者: CHYME 時間: 2025-3-31 17:47
A Static Headspace Gas Chromatography Method for Quantitation of Residual Solvents in Nanoformulatiospecific, linear, accurate, precise, and sensitive for the analyzed solvents. The method is suitable for the analysis of 13 residual solvents (methanol, ethanol, acetone, diethyl ether, 2-propanol, acetonitrile, 1-propanol, ethyl acetate, tetrahydrofuran, dichloromethane, chloroform, 1-butanol, and 作者: Subdue 時間: 2025-3-31 21:50 作者: NAVEN 時間: 2025-4-1 03:33
Detection of Beta-Glucan Contamination in Nanoparticle Formulationshe risk of immunogenicity. The protocol for the detection and quantification of β-(1,3)-D-glucans in nanoparticle formulations is based on a modified limulus amoebocyte lysate assay. The results of this test are used to inform immunotoxicity studies of nanotechnology-based drug products.作者: Limpid 時間: 2025-4-1 08:09 作者: prodrome 時間: 2025-4-1 13:50 作者: Deadpan 時間: 2025-4-1 14:52
Book 2024Latest editionn the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step instructions to reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cut
