標(biāo)題: Titlebook: Chaperones; Methods and Protocol Stuart K. Calderwood,Thomas L. Prince Book 2023Latest edition The Editor(s) (if applicable) and The Author [打印本頁] 作者: ARSON 時(shí)間: 2025-3-21 19:02
書目名稱Chaperones影響因子(影響力)
書目名稱Chaperones影響因子(影響力)學(xué)科排名
書目名稱Chaperones網(wǎng)絡(luò)公開度
書目名稱Chaperones網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Chaperones被引頻次
書目名稱Chaperones被引頻次學(xué)科排名
書目名稱Chaperones年度引用
書目名稱Chaperones年度引用學(xué)科排名
書目名稱Chaperones讀者反饋
書目名稱Chaperones讀者反饋學(xué)科排名
作者: Sciatica 時(shí)間: 2025-3-21 21:45 作者: 拒絕 時(shí)間: 2025-3-22 04:29 作者: 頌揚(yáng)本人 時(shí)間: 2025-3-22 06:59
Detecting Posttranslational Modifications of Hsp90 Isoforms,modifications (such as phosphorylation, SUMOylation, and ubiquitination) are important for Hsp90 stability and regulation of its ATPase activity. Both mammalian and yeast cells can be used to express and purify Hsp90 and TRAP1 and also detect post-translational modifications by immunoblotting.作者: 委派 時(shí)間: 2025-3-22 08:43 作者: 橡子 時(shí)間: 2025-3-22 15:09 作者: 橡子 時(shí)間: 2025-3-22 20:50 作者: FELON 時(shí)間: 2025-3-22 22:01
Book 2023Latest editionps on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Chaperones: Methods and Protocols, Second Edition. is a valuable resource for all researchers who want to learn more about this interesting and developing field.?.作者: delusion 時(shí)間: 2025-3-23 05:00 作者: 裂口 時(shí)間: 2025-3-23 07:38
Practical Ajax Projects with Java Technologym isolated nuclei and sheared by sonication. Hsp90-bound loci are immunoprecipitated and the corresponding DNA fragments are purified and sequenced. The described approach revealed that Hsp90 binds close to the transcriptional start site of around one-third of all . coding genes and characterized the role of the chaperone at chromatin.作者: 終點(diǎn) 時(shí)間: 2025-3-23 11:31 作者: Eructation 時(shí)間: 2025-3-23 15:44 作者: DOTE 時(shí)間: 2025-3-23 20:40 作者: Commonplace 時(shí)間: 2025-3-24 01:04 作者: 拉開這車床 時(shí)間: 2025-3-24 06:25 作者: menopause 時(shí)間: 2025-3-24 08:01 作者: Constitution 時(shí)間: 2025-3-24 12:10
Targeted Replacement of HSF1 Phosphorylation Sites at S303/S307 with Alanine Residues in Mice Incre mouse model indicated the susceptibility of the mutant mice to become obese with age due to an increase in basal levels of heat shock proteins (HSPs) and chronic inflammation. Since HSF1 transcriptional activity is increased in many tumor types, this mouse model may be a useful tool for studies related to cellular transformation and cancer.作者: Solace 時(shí)間: 2025-3-24 16:08
Complementation Assays for Co-chaperone Function,functionality of eukaryotic JDPs can be assessed in a thermosensitive . mutant strain of the yeast . (JJ160). Here we outline the use of these thermosensitive microorganisms in complementation assays to functionally characterize a JDP from the bacterium, . (AgtDnaJ), and a JDP from the trypanosomal parasite, . (TcJ2).作者: anaphylaxis 時(shí)間: 2025-3-24 20:33
Optimized Microscale Protein Aggregation Suppression Assay: A Method for Evaluating the Holdase Actnce-based miniaturized versions of the standard aggregation suppression assay that are affordable and have wide application for low abundance holdases. The assay can be used for both fundamental characterization of holdase function in proteins and screening of inhibitors of holdase activity.作者: intention 時(shí)間: 2025-3-24 23:13 作者: Inferior 時(shí)間: 2025-3-25 06:28
1064-3745 ation advice from the expertsThis second edition volume expands on the previous edition with new discussions on the latest techniques used to study molecular chaperones and the stress response. The chapters in this book cover such as analysis of the initiation and regulation of the stress response; 作者: 裹住 時(shí)間: 2025-3-25 11:25 作者: 轉(zhuǎn)換 時(shí)間: 2025-3-25 14:46 作者: 調(diào)整校對(duì) 時(shí)間: 2025-3-25 16:57
Natural Numbers Just Aren’t Enoughme study necessitates the implementation of methods that retain these protein complexes in their native cellular states for analysis. Here we describe a protocol for detection and composition analysis of epichaperomes in cell homogenates through native polyacrylamide gel electrophoresis.作者: 抵消 時(shí)間: 2025-3-25 20:06 作者: 條約 時(shí)間: 2025-3-26 03:25 作者: Ingest 時(shí)間: 2025-3-26 05:18
Transfection and Thermotolerance Methods for Analysis of miR-570 Targeting the HSP Chaperone Networcer. We identified a potent microRNA, miR-570 that could bind the 3′untranslated regions of multiple HSP mRNAs and inhibit HSP synthesis. Here, we will introduce the transfection and thermotolerance methods for analysis of miR-570 targeting the HSP chaperone network.作者: 全能 時(shí)間: 2025-3-26 09:53 作者: 聲音刺耳 時(shí)間: 2025-3-26 14:51 作者: BOOR 時(shí)間: 2025-3-26 19:02 作者: Prostaglandins 時(shí)間: 2025-3-26 21:01
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/223941.jpg作者: 閃光東本 時(shí)間: 2025-3-27 01:26 作者: 譏諷 時(shí)間: 2025-3-27 08:28 作者: 分開 時(shí)間: 2025-3-27 10:48
Chaperones978-1-0716-3342-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: RECUR 時(shí)間: 2025-3-27 16:30
InstaMail: An Ajax-Based Webmail Cliention involves primarily a family of heat shock transcription factors (HSF) that bind the heat shock elements of the . genes and mediate transcription .. We discuss methods for the study of HSP binding to . promoters and the consequent increases in . gene expression in vitro and in vivo.作者: 飛來飛去真休 時(shí)間: 2025-3-27 21:33
The Server Side of the Equation is regulated by heat shock factor 1 (HSF1). Activation of HSF1 induces the expression of heat shock proteins (HSPs) that function as molecular chaperones to fold and maintain the three-dimensional structure of misfolded proteins. The regulation of the degree and duration of the HSR is controlled by作者: eczema 時(shí)間: 2025-3-27 23:22
Practical Ajax Projects with Java Technologyc acid-associated or specific proteins can be identified and quantified by techniques such as mass spectrometry and immunoblotting. Here, a modified immobilized template assay combined with in vitro transcription assay to study the function of transcription factors and transcriptional activities at 作者: aesthetic 時(shí)間: 2025-3-28 03:26
InstaMail: An Ajax-Based Webmail Cliention involves primarily a family of heat shock transcription factors (HSF) that bind the heat shock elements of the . genes and mediate transcription .. We discuss methods for the study of HSP binding to . promoters and the consequent increases in . gene expression in vitro and in vivo.作者: puzzle 時(shí)間: 2025-3-28 08:13
Practical Ajax Projects with Java Technologyranscripts. RNA-seq can be used for differential gene expression (DGE) analysis, gene fusion detection, allele-specific expression, isoform and splice variant quantification, and identification of novel genes. These applications can be used for downstream systems biology analyses such as gene ontolo作者: 白楊 時(shí)間: 2025-3-28 13:34 作者: gorgeous 時(shí)間: 2025-3-28 16:16 作者: Project 時(shí)間: 2025-3-28 21:35 作者: pulse-pressure 時(shí)間: 2025-3-29 00:20
https://doi.org/10.1007/978-1-4471-0127-7 between molecular chaperones, co-chaperones, and client proteins. Consequently, strategies to visualize and analyze PPI in cells are useful in understanding protein homeostasis regulation. The Bimolecular Fluorescence Complementation (BiFC) assay has emerged as a useful tool for studying PPI betwee作者: 津貼 時(shí)間: 2025-3-29 03:24 作者: Between 時(shí)間: 2025-3-29 08:15
Practical Analysis in One Variables an amenable method to determine chaperone activity. The holdase function is reliably and easily achieved by monitoring the suppression of heat-induced aggregation of well-characterized model protein substrates. However, the standard assay format requires large amounts of protein and hence is not a作者: deviate 時(shí)間: 2025-3-29 11:24 作者: Stricture 時(shí)間: 2025-3-29 17:12 作者: dagger 時(shí)間: 2025-3-29 23:20 作者: 嘲弄 時(shí)間: 2025-3-30 00:11
Natural Numbers Just Aren’t Enoughous protein–protein interactions inside cells, which aberrantly affects the function of protein networks, and in turn, cellular phenotypes. Epichaperome study necessitates the implementation of methods that retain these protein complexes in their native cellular states for analysis. Here we describe作者: FRAX-tool 時(shí)間: 2025-3-30 04:45
Undergraduate Texts in Mathematicsxpressed on a wide range of cell types, including immune cells. We have investigated the nature of such proteins by cloning candidate receptors into cells (CHO-K1) with the rare property of being null for HSP binding. Using this approach, we have discovered that mammalian and eukaryotic Hsp70 binds 作者: 過度 時(shí)間: 2025-3-30 08:56
Fixed Points and Contraction Mapsitic cells (Hsp70.PC-F). In this approach, the tumor antigens are introduced into the antigen-processing machinery of dendritic cells through the cell fusion process, and thus we can obtain antigenic tumor peptides or their intermediates that have been processed by dendritic cells. Our results show 作者: 有常識(shí) 時(shí)間: 2025-3-30 12:51
Natural Numbers Just Aren’t Enoughvided significant supporting evidence that implicates eHsp90 both in normal cellular processes such as wound healing and in the development of human pathologies and diseases including fibrosis and cancer. In the early 2000s, Eustace et al. demonstrated that eHsp90 promotes the invasion of breast can作者: Indict 時(shí)間: 2025-3-30 19:09 作者: Medicaid 時(shí)間: 2025-3-30 22:36 作者: Verify 時(shí)間: 2025-3-31 01:42
Role of Heat Shock Factors in Stress-Induced Transcription: An Update,ion involves primarily a family of heat shock transcription factors (HSF) that bind the heat shock elements of the . genes and mediate transcription .. We discuss methods for the study of HSP binding to . promoters and the consequent increases in . gene expression in vitro and in vivo.作者: 一再困擾 時(shí)間: 2025-3-31 09:06 作者: BORE 時(shí)間: 2025-3-31 10:19
Studying RNA Polymerase II Promoter-Proximal Pausing by In Vitro Immobilized Template and Transcripc acid-associated or specific proteins can be identified and quantified by techniques such as mass spectrometry and immunoblotting. Here, a modified immobilized template assay combined with in vitro transcription assay to study the function of transcription factors and transcriptional activities at 作者: 公社 時(shí)間: 2025-3-31 16:04 作者: 定點(diǎn) 時(shí)間: 2025-3-31 21:18 作者: conduct 時(shí)間: 2025-3-31 22:49 作者: 鍵琴 時(shí)間: 2025-4-1 04:29
Transfection and Thermotolerance Methods for Analysis of miR-570 Targeting the HSP Chaperone Networtwork of chaperone interactions, known as the chaperome, contributes significantly to the proteotoxic cell response and the malignant phenotype in cancer. We identified a potent microRNA, miR-570 that could bind the 3′untranslated regions of multiple HSP mRNAs and inhibit HSP synthesis. Here, we wil作者: 有常識(shí) 時(shí)間: 2025-4-1 08:15 作者: 可能性 時(shí)間: 2025-4-1 11:51 作者: Pillory 時(shí)間: 2025-4-1 18:07
Complementation Assays for Co-chaperone Function,s for testing the co-chaperone function of JDPs. In these assays, an exogenously expressed novel JDP is tested for its ability to functionally substitute for a non-expressed or nonfunctional endogenous JDP(s) by reversing a stress phenotype. For example, the in vivo functionality of prokaryotic JDPs作者: vitrectomy 時(shí)間: 2025-4-1 22:28 作者: 自負(fù)的人 時(shí)間: 2025-4-1 23:31
Detecting Posttranslational Modifications of Hsp90 Isoforms,ep carcinogenesis. There are multiple Hsp90 isoforms including the cytosolic Hsp90α and Hsp90β as well as GRP94 located in the endoplasmic reticulum and TRAP1 in the mitochondria. The chaperone function of Hsp90 is linked to its ability to bind and hydrolyze ATP. Co-chaperones and posttranslational
