標(biāo)題: Titlebook: Cervical Cancer; Methods and Protocol Daniel Keppler,Athena W. Lin Book 2015 Springer Science+Business Media New York 2015 Cervix.CxCA.Drug [打印本頁] 作者: 小故障 時間: 2025-3-21 19:04
書目名稱Cervical Cancer影響因子(影響力)
書目名稱Cervical Cancer影響因子(影響力)學(xué)科排名
書目名稱Cervical Cancer網(wǎng)絡(luò)公開度
書目名稱Cervical Cancer網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cervical Cancer被引頻次
書目名稱Cervical Cancer被引頻次學(xué)科排名
書目名稱Cervical Cancer年度引用
書目名稱Cervical Cancer年度引用學(xué)科排名
書目名稱Cervical Cancer讀者反饋
書目名稱Cervical Cancer讀者反饋學(xué)科排名
作者: 側(cè)面左右 時間: 2025-3-22 00:03
Planar Parallel Robotic Machine Design,cle of HPV is tied to the epithelial differentiation system, as only native virus can be produced in stratified human skin. Initially, HPV research was only possible utilizing recombinant systems in monolayer culture. With new cell culture technology, systems using differentiated skin have allowed H作者: Demulcent 時間: 2025-3-22 00:43
Planar Parallel Robotic Machine Design,ssay described here is an important tool to study HPV receptor binding to the ECM. The assay can also be modified to study the receptors required for HPV infection and for binding to tissues. We previously showed that Ln-332 is essential for the binding of HPV11 to human keratinocytes and that infec作者: configuration 時間: 2025-3-22 07:57
Planar Parallel Robotic Machine Design,njunction with the host DNA replication machinery. This process is tightly linked to the replication of cellular DNA, in part through the cyclin-dependent phosphorylation of E1, which inhibits its export out of the nucleus to promote its accumulation in this compartment during S-phase. It has been r作者: evince 時間: 2025-3-22 09:47
Architectures of Parallel Robotic Machine,nderstand HPV biology during the normal, differentiation-dependent life cycle, a cell culture model that maintains the complete episomal genome and permits host cell differentiation is critical. Furthermore, the use of cloned DNA as a starting material is important to facilitate genetic analyses. In作者: mitten 時間: 2025-3-22 16:23
Architectures of Parallel Robotic Machine,tocols for robust production of HPV-18 in organotypic cultures of early passages of primary human keratinocytes. A critical step is the generation of genomic HPV plasmids in vivo by using Cre-loxP-mediated excisional recombination from a vector plasmid. We discuss the rationale for this approach. Th作者: mitten 時間: 2025-3-22 20:28
https://doi.org/10.1007/1-4020-4133-0genital region and oral mucosa. The capacity of these viruses to induce cancer and to immortalize cells in culture relies in part on a critical function of their E6 oncoprotein, that of promoting the poly-ubiquitination of the cellular tumor suppressor protein p53 and its subsequent degradation by t作者: 600 時間: 2025-3-23 00:35 作者: Interferons 時間: 2025-3-23 04:48 作者: Dysarthria 時間: 2025-3-23 08:58
Structural synthesis and architectures, of cancer-related death. Therefore, CSC-targeted cancer therapy is important for the future development of more effective and advanced cancer therapy. One of the approaches is to specifically silence oncogene expression in CSCs and inhibit their growth. The significance of this approach is its spec作者: Nausea 時間: 2025-3-23 12:27 作者: cavity 時間: 2025-3-23 17:16
https://doi.org/10.1007/1-4020-4133-0d as novel chemotherapeutic agents. In this chapter, we describe an enzyme activity assay to assess the selectivity of a putative small-molecule DUB inhibitor toward a subset of DUBs in cancer cell lines.作者: 講個故事逗他 時間: 2025-3-23 19:52 作者: 某人 時間: 2025-3-24 00:57
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/223362.jpg作者: Blatant 時間: 2025-3-24 06:11 作者: Lamina 時間: 2025-3-24 07:31
Evolution and Classification of Oncogenic Human Papillomavirus Types and Variants Associated with CeThe nomenclature of human papillomavirus (HPV) is established by the International Committee on Taxonomy of Virus (ICTV). However, the ICTV does not set standards for HPV below species levels. This chapter describes detailed genotyping methods for determining and classifying HPV variants.作者: 偶像 時間: 2025-3-24 11:43 作者: apropos 時間: 2025-3-24 15:32
978-1-4939-5285-4Springer Science+Business Media New York 2015作者: 燦爛 時間: 2025-3-24 21:46
Cervical Cancer978-1-4939-2013-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Inferior 時間: 2025-3-25 02:58
Planar Parallel Robotic Machine Design,t way, LiPA analysis of the HPV-negative samples can be avoided, reducing workload and cost. Here, we describe in detail a SYBR Green I-based real-time PCR assay based on SPF10 primers using the LightCycler. 480 system to generate and detect HPV amplicons, which are compatible with the LiPA assay.作者: 疾馳 時間: 2025-3-25 05:51 作者: 名字的誤用 時間: 2025-3-25 09:04
https://doi.org/10.1007/1-4020-4133-0d as novel chemotherapeutic agents. In this chapter, we describe an enzyme activity assay to assess the selectivity of a putative small-molecule DUB inhibitor toward a subset of DUBs in cancer cell lines.作者: Brittle 時間: 2025-3-25 12:22
A Real-Time PCR Approach Based on SPF10 Primers and the INNO-LiPA HPV Genotyping Extra Assay for thet way, LiPA analysis of the HPV-negative samples can be avoided, reducing workload and cost. Here, we describe in detail a SYBR Green I-based real-time PCR assay based on SPF10 primers using the LightCycler. 480 system to generate and detect HPV amplicons, which are compatible with the LiPA assay.作者: 啜泣 時間: 2025-3-25 18:28 作者: ENNUI 時間: 2025-3-25 21:35 作者: 無目標(biāo) 時間: 2025-3-26 03:47
HPV Binding Assay to Laminin-332/Integrin α6β4 on Human Keratinocyteshanisms will aid in the design of therapeutics against HPVs and ultimately help prevent many cancers. In this chapter, we describe the HPV binding assay to Ln-332/integrin α6β4 on human keratinocytes (ECM). We also present data and suggestions for modifying the assay for testing the specificity of H作者: 協(xié)迫 時間: 2025-3-26 04:45
A High-Throughput Cellular Assay to Quantify the p53-Degradation Activity of E6 from Different Humaned cells by determining the amount of E6-expression vector required to reduce by half the levels of RLuc-p53 luciferase activity (50 % effective concentration [EC.]). The high-throughput and quantitative nature of this assay makes it particularly useful to compare the p53-degradation activities of E作者: 放逐 時間: 2025-3-26 10:29 作者: 一大群 時間: 2025-3-26 15:50 作者: Factual 時間: 2025-3-26 16:51 作者: 榨取 時間: 2025-3-26 22:11
Planar Parallel Robotic Machine Design,hanisms will aid in the design of therapeutics against HPVs and ultimately help prevent many cancers. In this chapter, we describe the HPV binding assay to Ln-332/integrin α6β4 on human keratinocytes (ECM). We also present data and suggestions for modifying the assay for testing the specificity of H作者: 系列 時間: 2025-3-27 01:26 作者: farewell 時間: 2025-3-27 07:17
Solid Mechanics and Its Applicationsdeveloped using siRNA expression plasmids. These vectors contain DNA inserts designed with software to generate highly efficient siRNAs which will assemble into RNA-induced silencing complexes (RISC), and silence the target mRNA. In addition, the DNA inserts may be contained in cloning cassettes, an作者: 慢慢啃 時間: 2025-3-27 12:29 作者: 蒙太奇 時間: 2025-3-27 15:45
Planar Parallel Robotic Machine Design,ous effects of its nuclear accumulation on cellular proliferation, cell cycle progression and the induction of a DDR, using a combination of colony formation assays, immunofluorescence microcopy, and flow cytometry approaches.作者: Nerve-Block 時間: 2025-3-27 20:07
Methods to Assess the Nucleocytoplasmic Shuttling of the HPV E1 Helicase and Its Effects on Cellularous effects of its nuclear accumulation on cellular proliferation, cell cycle progression and the induction of a DDR, using a combination of colony formation assays, immunofluorescence microcopy, and flow cytometry approaches.作者: Permanent 時間: 2025-3-28 00:30
Architectures of Parallel Robotic Machine, this chapter, procedures for isolating human keratinocytes, establishing cell lines maintaining HPV16 genomes, and inducing cellular differentiation, which permits analysis of both early and late stages in the viral life cycle, are described.作者: Enthralling 時間: 2025-3-28 03:45 作者: 真實(shí)的人 時間: 2025-3-28 09:40 作者: 兇猛 時間: 2025-3-28 10:29
Structural synthesis and architectures,. One of the approaches is to specifically silence oncogene expression in CSCs and inhibit their growth. The significance of this approach is its specificity and ability to avoid multi-drug resistance of CSCs. In this chapter, we will describe a method of silencing HPV oncogenes E6/E7 in human cervical CSCs using HeLa cells as a model system.作者: 抗生素 時間: 2025-3-28 18:01 作者: 尖酸一點(diǎn) 時間: 2025-3-28 21:04 作者: 蕁麻 時間: 2025-3-28 23:48 作者: BRAWL 時間: 2025-3-29 05:07 作者: GRUEL 時間: 2025-3-29 09:27 作者: 教義 時間: 2025-3-29 11:35
Retroviral Expression of Human Cystatin Genes in HeLa Cellsucts that have negative effect on cell growth and viability. Here, we describe the retroviral transfer of cystatin cDNAs using HEK293-derived Phoenix packaging cells and human HeLa cervical carcinoma cells as target cells.作者: llibretto 時間: 2025-3-29 18:27 作者: 馬具 時間: 2025-3-29 21:25 作者: Harpoon 時間: 2025-3-30 01:37
A Real-Time PCR Approach Based on SPF10 Primers and the INNO-LiPA HPV Genotyping Extra Assay for thet way, LiPA analysis of the HPV-negative samples can be avoided, reducing workload and cost. Here, we describe in detail a SYBR Green I-based real-time PCR assay based on SPF10 primers using the LightCycler. 480 system to generate and detect HPV amplicons, which are compatible with the LiPA assay.作者: essential-fats 時間: 2025-3-30 07:21 作者: 鴕鳥 時間: 2025-3-30 10:06 作者: Dignant 時間: 2025-3-30 14:09 作者: 迅速飛過 時間: 2025-3-30 17:00
Genetic Methods for Studying the Role of Viral Oncogenes in the HPV Life Cyclenderstand HPV biology during the normal, differentiation-dependent life cycle, a cell culture model that maintains the complete episomal genome and permits host cell differentiation is critical. Furthermore, the use of cloned DNA as a starting material is important to facilitate genetic analyses. In作者: 刪除 時間: 2025-3-30 21:28 作者: HPA533 時間: 2025-3-31 03:27 作者: jumble 時間: 2025-3-31 05:59
