標(biāo)題: Titlebook: Cereal Genomics; Methods and Protocol Luis M. Vaschetto Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 High-t [打印本頁(yè)] 作者: 街道 時(shí)間: 2025-3-21 18:06
書目名稱Cereal Genomics影響因子(影響力)
書目名稱Cereal Genomics影響因子(影響力)學(xué)科排名
書目名稱Cereal Genomics網(wǎng)絡(luò)公開度
書目名稱Cereal Genomics網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cereal Genomics被引頻次
書目名稱Cereal Genomics被引頻次學(xué)科排名
書目名稱Cereal Genomics年度引用
書目名稱Cereal Genomics年度引用學(xué)科排名
書目名稱Cereal Genomics讀者反饋
書目名稱Cereal Genomics讀者反饋學(xué)科排名
作者: BULLY 時(shí)間: 2025-3-21 20:54 作者: FLAIL 時(shí)間: 2025-3-22 02:36 作者: 增長(zhǎng) 時(shí)間: 2025-3-22 07:46
https://doi.org/10.1007/978-3-658-26123-8utine and efficient rice transformation protocols by .. This method uses mature seeds as a material and can be applied to many . and some other varieties of rice. According to this method, it is even possible for beginners to obtain rice transformants.作者: Morbid 時(shí)間: 2025-3-22 11:30
Cost-Effective Profiling of , Transposon Insertions in Maize by Next-Generation Sequencing,particularly high duplication frequency, which results in large numbers of new mutations in lineages that carry active . elements. Here we describe a rapid and cost-effective Miseq-based . transposon profiling pipeline. This method can also be used for identifying flanking sequences of other types of long insertions such as T-DNAs.作者: 四目在模仿 時(shí)間: 2025-3-22 14:08
Relative Expression Analysis of Target Genes by Using Reverse Transcription-Quantitative PCR,le and efficient protocol to determine gene expression in cereals, based on real-time PCR using SYBR. Green dye. This technique provide an inexpensive alternative, since no probes are required, allowing for the quantification of a high number of genes with reduced cost.作者: 四目在模仿 時(shí)間: 2025-3-22 20:27
Transformation in the Rice Genome,utine and efficient rice transformation protocols by .. This method uses mature seeds as a material and can be applied to many . and some other varieties of rice. According to this method, it is even possible for beginners to obtain rice transformants.作者: OGLE 時(shí)間: 2025-3-22 23:28 作者: GROVE 時(shí)間: 2025-3-23 02:27
Sequencing and Assembling Genomes and Chromosomes of Cereal Crops,ologies for sequencing and assembly of flow sorted chromosomes and whole genomes in cereal crops, with special emphasis on the case of the International Wheat Genome Sequencing Consortium (IWGSC), are reviewed.作者: PAN 時(shí)間: 2025-3-23 06:21
Genome-Wide Identification of Regulatory DNA Elements in Crop Plants,n both plants and animals. Here we describe a DNase-seq procedure modified and developed for crop plants. These plants usually contain large amounts of repetitive sequences and complex organic constituents. With the main improvement in nuclei isolation, this method has been successfully used in mapping DHSs in cotton and sugarcane.作者: 背心 時(shí)間: 2025-3-23 13:07
Genome-Wide Profiling of Histone Modifications with ChIP-Seq,s targeting the histone modification of interest are incubated with the sheared chromatin and nonspecific interactions are washed away. DNA is purified via phenol-chloroform extraction, end-repaired, ligated to sequencing adapters, and PCR-amplified.作者: 侵略者 時(shí)間: 2025-3-23 13:54 作者: synovium 時(shí)間: 2025-3-23 20:54 作者: 非秘密 時(shí)間: 2025-3-24 02:15 作者: 繁重 時(shí)間: 2025-3-24 03:56
CRISPR/Cas9-Mediated Targeted Mutagenesis in Wheat Doubled Haploids,get for gene editing experiments, as they enable generation of homozygous doubled haploid mutants in one generation. Here, we describe optimized methods for genome editing of haploid wheat microspores and production of doubled haploid plants by microspore culture.作者: FLORA 時(shí)間: 2025-3-24 07:30 作者: daredevil 時(shí)間: 2025-3-24 12:31 作者: nominal 時(shí)間: 2025-3-24 18:22 作者: 讓你明白 時(shí)間: 2025-3-24 22:59
A singular stochastic control problem,s targeting the histone modification of interest are incubated with the sheared chromatin and nonspecific interactions are washed away. DNA is purified via phenol-chloroform extraction, end-repaired, ligated to sequencing adapters, and PCR-amplified.作者: 憤怒事實(shí) 時(shí)間: 2025-3-25 00:10
https://doi.org/10.1007/978-3-658-25691-3hylated cytosine to uracil, whereas methylated cytosine is unchanged. The bisulfite conversion of whole genome sequencing libraries before the final amplification step allows for the discrimination of methylated from unmethylated cytosines in a genome-wide manner.作者: BILL 時(shí)間: 2025-3-25 06:08 作者: cortisol 時(shí)間: 2025-3-25 10:18
https://doi.org/10.1007/978-3-658-25744-6insights into the functional roles of circRNAs can be used by cereal scientists and molecular breeders with the aim to develop new strategies for crop improvement. Here, we briefly outline the current knowledge about circRNAs in plants and we also outline available computational resources for their validation and analysis in cereal species.作者: GET 時(shí)間: 2025-3-25 15:20 作者: Morphine 時(shí)間: 2025-3-25 19:02
Book 2020 in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping,?next-sequencing generation, transposable element-associated variation, transcriptom作者: beta-cells 時(shí)間: 2025-3-25 22:52 作者: Yourself 時(shí)間: 2025-3-26 03:07 作者: 身心疲憊 時(shí)間: 2025-3-26 05:47 作者: 意見一致 時(shí)間: 2025-3-26 12:06 作者: Chagrin 時(shí)間: 2025-3-26 14:29 作者: finale 時(shí)間: 2025-3-26 17:13
Online-Reputationskompetenz von Mitarbeiternn analysis and demonstrated its utility to rapidly narrow down previously identified QTL intervals to few candidate genes. Here, we describe the detailed step-by-step guide for performing MetaQTL specific regional association analysis to identify important genomic regions and underlying potential ma作者: 約會(huì) 時(shí)間: 2025-3-26 23:34 作者: 音的強(qiáng)弱 時(shí)間: 2025-3-27 03:24 作者: obtuse 時(shí)間: 2025-3-27 05:55 作者: goodwill 時(shí)間: 2025-3-27 10:24 作者: meditation 時(shí)間: 2025-3-27 15:57 作者: 不能強(qiáng)迫我 時(shí)間: 2025-3-27 21:15
Emerging Genome Engineering Tools in Crop Research and Breeding,to gene editing technologies, improvement in delivery systems of exogenous DNA into plant cells have increased the rate of successful gene editing events. Regeneration of fertile plants containing the desired edits remains challenging; however, manipulation of embryogenesis-related genes such as BAB作者: 產(chǎn)生 時(shí)間: 2025-3-28 01:49
In Vivo Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isofo PEPC, whereas the latter antibodies had a broader specificity for the phosphorylated PEPC in various plant species. The following procedures are described herein: (1) preparation of the phosphopeptide and phosphonopeptide; (2) preparation and purification of rabbit antibodies; (3) preparation of ce作者: intricacy 時(shí)間: 2025-3-28 02:19 作者: 就職 時(shí)間: 2025-3-28 08:49 作者: IOTA 時(shí)間: 2025-3-28 13:57 作者: 谷物 時(shí)間: 2025-3-28 18:11 作者: DRILL 時(shí)間: 2025-3-28 20:31 作者: Maximize 時(shí)間: 2025-3-29 01:58 作者: acolyte 時(shí)間: 2025-3-29 03:21 作者: 陪審團(tuán) 時(shí)間: 2025-3-29 10:04
DNA Methylation and Transcriptomic Next-Generation Technologies in Cereal Genomics,diated by DNA methylation. Here we describe a bioinformatics protocol to analyze gene expression levels using RNA-seq data that allow us to identify candidate genes to be tested by bisulfite assays. The candidate methylated genes are usually those that are low expressed in a particular condition or 作者: 不適當(dāng) 時(shí)間: 2025-3-29 13:45
Genome-Wide Identification of Regulatory DNA Elements in Crop Plants,h sensitive to DNase I digestion, which are termed as DNase I hypersensitive sites (DHSs). These DHSs can be efficiently detected through DNase I digestion followed by high-throughput DNA sequencing (DNase-seq). Thus, DNase-seq has become a powerful technique for DHSs mapping at whole-genome level i作者: 割讓 時(shí)間: 2025-3-29 19:08
Genome-Wide Profiling of Histone Modifications with ChIP-Seq, proteins. This protocol has been developed and utilized to perform ChIP on histone covalent modifications in various plant species including cereals. DNA and chromatin-associated proteins are crosslinked with formaldehyde. Chromatin is then isolated from nuclei and sheared via sonication. Antibodie作者: Left-Atrium 時(shí)間: 2025-3-29 20:47 作者: NEEDY 時(shí)間: 2025-3-30 02:20 作者: Dedication 時(shí)間: 2025-3-30 06:39 作者: Discrete 時(shí)間: 2025-3-30 09:13
Cereal Circular RNAs (circRNAs): An Overview of the Computational Resources for Identification and ogical functions in all kingdoms of life. Recently, circRNAs have been identified in many plant species, including cereal crops, showing differential expression during stress response and developmental programs, which suggests their role in these process. In the following years, it is expected that 作者: Yourself 時(shí)間: 2025-3-30 14:35
Emerging Genome Engineering Tools in Crop Research and Breeding,netic material creates opportunities for rapid development of elite cultivars with desired traits. The plant genome can be altered in several ways, including targeted introduction of nucleotide changes, deleting DNA segments, introducing exogenous DNA fragments and epigenetic modifications. Targeted作者: semiskilled 時(shí)間: 2025-3-30 18:09
CRISPR/Cas9-Mediated Targeted Mutagenesis in Wheat Doubled Haploids,o conventional transformation methods. Additionally, the long generation time of crop plants poses a significant obstacle to effective application of gene editing technology, as it takes a long time to produce modified homozygous genotypes. The haploid single-celled microspores are an attractive tar作者: Ophthalmoscope 時(shí)間: 2025-3-31 00:01
Genetic Transformation of Protoplasts Isolated from Leaves of , and ,lized to screen potential sgRNA guides for CRISPR-mediated genome editing. However, little research has been conducted into the use of transient expression of protoplasts in . (a globally important pasture, hay, and amenity grass), and no studies have been conducted into . (a weed in cereal crops bu作者: BRACE 時(shí)間: 2025-3-31 02:18
Transformation in the Rice Genome,ished by vigorous studies on the culture system and the elucidation of . transformation mechanisms. This section provides a detailed description of routine and efficient rice transformation protocols by .. This method uses mature seeds as a material and can be applied to many . and some other variet作者: 責(zé)任 時(shí)間: 2025-3-31 07:51
In Vivo Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isofo by reversible phosphorylation in vascular plants. The phosphorylation site on a PEPC molecule is conserved not only among isoforms but also across plant species. An anti-phosphopeptide antibody is a common and powerful tool for detecting phosphorylated target proteins with high specificity. We gene作者: Ballerina 時(shí)間: 2025-3-31 10:08 作者: 共棲 時(shí)間: 2025-3-31 14:53
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/223261.jpg作者: Neuropeptides 時(shí)間: 2025-3-31 20:14
Cereal Genomics978-1-4939-9865-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 噴出 時(shí)間: 2025-4-1 00:20
https://doi.org/10.1007/978-3-658-25677-7diated by DNA methylation. Here we describe a bioinformatics protocol to analyze gene expression levels using RNA-seq data that allow us to identify candidate genes to be tested by bisulfite assays. The candidate methylated genes are usually those that are low expressed in a particular condition or developmental stage.作者: 圓木可阻礙 時(shí)間: 2025-4-1 04:03 作者: 任意 時(shí)間: 2025-4-1 06:33 作者: 空洞 時(shí)間: 2025-4-1 10:38 作者: 亞當(dāng)心理陰影 時(shí)間: 2025-4-1 15:47
https://doi.org/10.1007/978-3-658-25360-8ends on several factors such as choice of starting material, ease of handling, time and labor required for isolation, the final quantity as well as the quality of genomic DNA. We outline here a high-throughput method of DNA extraction from different plant species including cereal crops. The protocol
