標題: Titlebook: Cellular and Molecular Aspects of the Plant Hormone Ethylene; Proceedings of the I Jean Claude Pech,Alain Latché,Claudine Balagué Conferenc [打印本頁] 作者: HAND 時間: 2025-3-21 18:16
書目名稱Cellular and Molecular Aspects of the Plant Hormone Ethylene影響因子(影響力)
書目名稱Cellular and Molecular Aspects of the Plant Hormone Ethylene影響因子(影響力)學科排名
書目名稱Cellular and Molecular Aspects of the Plant Hormone Ethylene網(wǎng)絡公開度
書目名稱Cellular and Molecular Aspects of the Plant Hormone Ethylene網(wǎng)絡公開度學科排名
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書目名稱Cellular and Molecular Aspects of the Plant Hormone Ethylene讀者反饋
書目名稱Cellular and Molecular Aspects of the Plant Hormone Ethylene讀者反饋學科排名
作者: 基因組 時間: 2025-3-21 22:45
Monomeric and Dimeric Forms and Mechanism-Based Inactivation of 1-Aminocyclopropane-1-Carboxylate Syato and winter squash ACC synthase, only the enzyme from tomato fruit tissue existed in a monomeric form, whereas the others in a dimeric form. The monomeric tomato ACC synthase was much less sensitive to the mechanism-based inactivation than the dimeric forms of ACC synthase. We suggest that ACC sy作者: neolith 時間: 2025-3-22 00:28 作者: 津貼 時間: 2025-3-22 04:59
Modifying Fruit Ripening by Suppressing Gene Expression2). This hydrocarbon gas is generally considered to be the fruit ripening hormone (2,3). Because of its effects on plant senescence, large losses of fruits and vegetables are incurred annually in the U.S. The losses are much greater in third world countries because of the lack of sufficient refriger作者: 手工藝品 時間: 2025-3-22 11:20 作者: 搖晃 時間: 2025-3-22 15:08 作者: 搖晃 時間: 2025-3-22 19:45
Maximising the Activity of the Ethylene-Forming Enzymeo loss of activity when the enzyme is stored at 0°C for 24 hr. When enzyme preparations from melon are desalted by passage through a column of Sephadex, activity is reduced. The lost activity can be restored by the inclusion of dilute NaOH to the reaction mixture, which gives a 6-fold stimulation of作者: vasospasm 時間: 2025-3-22 23:52
Purification, Characterization and Subcellular Localization of ACC Oxidase from Fruits been determined. Antibodies raised against a recombinant ACC oxidase from pTOM13 cDNA recognise enzyme of apple fruit with high specificity. The enzyme appears to be non glycosylated. Evidence is given by various methods, including immunocytology, that most of ACC oxidase of ripening fruits was loc作者: Sarcoma 時間: 2025-3-23 01:43
Purification and Characterization of ACC Oxidase and Its Expression during Ripening in Apple Fruitcorbate is required as a cosubstrate (K. = 0.4 mM); L-ascorbic acid-6-palmitate inhibited ACC oxidase competitively (K. = 20.M) with respect to L-ascorbate. The enzyme is markedly activated by CO. (K. = 0.65%). Fe. is required (K. = 3.M); Co. is inhibitory (K. = 3.M) competitively with respect to Fe作者: Anticoagulant 時間: 2025-3-23 06:26
Mechanistic Aspects of ACC Oxidation to Ethyleneer being that probably occurring .. An essential feature of this reaction is the production of ACC-free radicals as intermediate. If these ACC-free radicals are liberated into the reaction medium, they will readily react with dissolved oxygen resulting in the formation of an ACC-derived hydroperoxid作者: archaeology 時間: 2025-3-23 10:13
Apple ACC Oxidase: Purification and Characterization of the Enzyme and Cloning of Its cDNAe migrated as a simple band of 35 kDa on SDS-PAGE and of 39 kDa on gel filtration. The enzyme activity, as ., required CO./HCO..; CO. being the active species. The purified enzyme displayed absolute requirement for Fe. and ascorbate. The stoichiometry of the reaction was determined to be: ACC + asco作者: 認為 時間: 2025-3-23 16:17
Biochemical and Molecular Characterization of Ethylene Forming Enzyme from Avocadopane-l-carboxylic acid (ACC). EFE extracted from avocado fruit was partially purified and shown to be a soluble, Fe(II) ascorbate-dependent enzyme. A ripening-related cDNA from avocado, pAVOe3, shows sequence similarity to several Fe(II) and ascorbate-dependent oxidases including EFE from tomato. Th作者: 亞當心理陰影 時間: 2025-3-23 21:59
Identification of Genes Encoding EFE in Tomatones and then by demonstrating their functional expression in a heterologous host. These results have shed much light on the nature of this enigmatic enzyme including its cellular location and mechanism of action.作者: pessimism 時間: 2025-3-23 23:11
EFE Multigene Family in Tomato Plants: Expression and Characterization. Previous screening of a tomato genomic library revealed three genomic clones, refered now to as ., . and .. The nucleotidic sequence of . is unknown while those of . and . have already been published. Moreover, . gene has been shown to encode pTOM 13 mRNA whereas no data were available so far conc作者: chondromalacia 時間: 2025-3-24 05:57 作者: Synovial-Fluid 時間: 2025-3-24 07:18
Conversion of 1-Aminocyclopropane-1-Carboxylic Acid to Ethylene and Its Regulation by Calcium in Suncomplex referred to as ethyleneforming-enzyme (EFE) (Yang and Hoffman,1984). The aims of the present work were to investigate the main characteristics and the regulation by calcium of EFE activity in protoplasts isolated from hypocotyls of sunflower (. L.) seedlings.作者: 低三下四之人 時間: 2025-3-24 11:25 作者: isotope 時間: 2025-3-24 14:53 作者: 反應 時間: 2025-3-24 22:10 作者: cipher 時間: 2025-3-25 00:38
Entbürokratisierte Pflegeplanungnthases have a property to form a dimer, but in tomato fruit tissue some modification takes place to the enzyme protein, which makes it remained as a monomer and less sensitive to the mechanism-based inactivation.作者: 態(tài)學 時間: 2025-3-25 04:05 作者: Hiatus 時間: 2025-3-25 11:28 作者: ROOF 時間: 2025-3-25 14:45
Rund um die Uhr — Zeitgestaltungs. A series of experiments with peach fruit were undertaken in order to understand the relationship of ethylene to ACC synthase RNA and EFE RNA and their relationships to softening. In experiments that decrease the amount of these RNAs in tomato fruit, ripening and softening are delayed (Hamilton et al. (1990), Oeller et al. (1991)).作者: SYN 時間: 2025-3-25 16:16
Betriebliche Gesundheitsf?rderung tomato plants expressing antisense EFE [Hamilton .., 1990] and ACC synthase [Oehler .., 1991] genes. In order to try and extend the storage life of melon fruit by inhibiting ethylene synthesis our initial aim was to isolate an EFE clone from a climacteric melon fruit cDNA library, using the heterologous pTOM13 (EFE) probe from tomato.作者: Interim 時間: 2025-3-25 23:30
Relationship of ACC Oxidase RNA, ACC Synthase RNA, and Ethylene in Peach Fruits. A series of experiments with peach fruit were undertaken in order to understand the relationship of ethylene to ACC synthase RNA and EFE RNA and their relationships to softening. In experiments that decrease the amount of these RNAs in tomato fruit, ripening and softening are delayed (Hamilton et al. (1990), Oeller et al. (1991)).作者: 平靜生活 時間: 2025-3-26 00:39
Isolation of a Ripening and Wound-Induced cDNA from , L.With Homology to the Ethylene Forming Enzyme tomato plants expressing antisense EFE [Hamilton .., 1990] and ACC synthase [Oehler .., 1991] genes. In order to try and extend the storage life of melon fruit by inhibiting ethylene synthesis our initial aim was to isolate an EFE clone from a climacteric melon fruit cDNA library, using the heterologous pTOM13 (EFE) probe from tomato.作者: zonules 時間: 2025-3-26 05:14
Entbürokratisierte Pflegeplanungynthesis is of fundamental as well as of applied significance. This update summarizes the recent advances in manipulating key genes in the ethylene biosynthetic pathway to prevent ethylene production and fruit ripening.作者: 艱苦地移動 時間: 2025-3-26 11:54
Rund um die Uhr — Zeitgestaltungby the root transformation method. Temporal and spatial regulation of expression of . were analyzed, revealing a developmental control both in shoot and root and confirming results obtained by RT-PCR analysis. A role for . in the developmental control of ethylene synthesis is suggested.作者: 完成 時間: 2025-3-26 12:47 作者: 鞭打 時間: 2025-3-26 18:14 作者: CODE 時間: 2025-3-26 23:52
Cloning and Expression Analysis of an , 1-Aminocyclopropane-1-Carboxylate Synthase Gene: Pattern of by the root transformation method. Temporal and spatial regulation of expression of . were analyzed, revealing a developmental control both in shoot and root and confirming results obtained by RT-PCR analysis. A role for . in the developmental control of ethylene synthesis is suggested.作者: 濃縮 時間: 2025-3-27 02:28
Immunocytolocalisation of ACC Oxidase in Tomato Fruitsbodies were purifed by affinity column chromatography and eventually saturated with excess of antigenes. Pericarp tissue of both normal and transgenic tomatoes transformed with antisense pTOM13 cDNA (kindly provided by A Hamilton and D. Grierson, Nottingham, UK) were processed for immunocytolocalization as described in [4].作者: esculent 時間: 2025-3-27 08:35
Monomeric and Dimeric Forms and Mechanism-Based Inactivation of 1-Aminocyclopropane-1-Carboxylate Synthases have a property to form a dimer, but in tomato fruit tissue some modification takes place to the enzyme protein, which makes it remained as a monomer and less sensitive to the mechanism-based inactivation.作者: mydriatic 時間: 2025-3-27 10:26
Maximising the Activity of the Ethylene-Forming Enzyme the EFE activity from melon. The EFE activity is also stimulated by the addition of HCO../CO. to the reaction mixture, with a 25-fold increase in activity for melon and an 8-fold increase in activity for apple.作者: 砍伐 時間: 2025-3-27 17:35 作者: 無聊點好 時間: 2025-3-27 20:43 作者: LIMN 時間: 2025-3-27 23:30
https://doi.org/10.1007/978-3-540-79332-8ery of EFE activity from the cell extracts. Using residue-specific chemical modification, a histidine critical for catalytic activity was detected. This is likely to be one of three histidine residues highly conserved among sequence-related Fe(II) ascorbate-dependent oxidases.作者: 暗諷 時間: 2025-3-28 05:05
Purification and Characterization of ACC Oxidase and Its Expression during Ripening in Apple Fruiteclimacteric apples. Immunoblots of proteins and of . translation products of poly(A)+ RNA from apples at progressive stages of the ethylene climacteric indicated that ACC oxidase and the mRNA encoding ACC oxidase was nil in unripe fruits but accumulated markedly during the ethylene climacteric.作者: 偶像 時間: 2025-3-28 06:37
Mechanistic Aspects of ACC Oxidation to Ethylenee (HPA), might occur. HPA has been found not only in stepwise-type reactions, such as those mediated by peroxidase, but also in concerted reactions, such as the oxidation by hypochlorite or hydrogen peroxide. In this work we investigate the mechanism of ACC oxidation by the two kinds of reactions and discuss some physiological implications.作者: CHYME 時間: 2025-3-28 12:30 作者: Ige326 時間: 2025-3-28 18:17
Conference proceedings 1993m August 31 st and September 4th, 1992. The planning and management of the scientific and social programme of the Conference were carried out jointly by the "Ethylene Research Group" of ENSAlIN"P (Toulouse) and Agropole Congres Service (Agen). Since the last meetings in Israel (1984) and in Belgium 作者: forebear 時間: 2025-3-28 20:01
Cellular and Molecular Aspects of the Plant Hormone EthyleneProceedings of the I作者: gregarious 時間: 2025-3-29 02:15 作者: 豐滿中國 時間: 2025-3-29 03:41
0924-1949 rspectives for the postharvest handling of plant products. Some progress has also been made on the understanding of ethylene action. However, much remains to be978-90-481-4249-1978-94-017-1003-9Series ISSN 0924-1949 作者: Osteoarthritis 時間: 2025-3-29 07:25 作者: reject 時間: 2025-3-29 11:35
Patienten- und Angeh?rigenarbeit vegetative hypocotyls as influenced by auxin treatment. Our results indicate that ACC synthase is encoded by a multigene family and each gene is differentially induced by developmental, environmental and physiological factors.作者: COLIC 時間: 2025-3-29 16:21 作者: 真 時間: 2025-3-29 20:57
Rund um die Uhr — Zeitgestaltungession at the RNA level. Gene specific oligonucleotides were designed and used as primers in PCR reactions to amplify about 200 bp of the 3’ untranslated regions. The amplified fragments were cloned in a transcription vector generating appropriate probes for subsequent study of the expression of EFE作者: 煩人 時間: 2025-3-30 00:35 作者: 形上升才刺激 時間: 2025-3-30 05:04 作者: Feckless 時間: 2025-3-30 08:27
Characterization of the 1-Aminocyclopropane-1-Carboxylate (ACC) Synthase Isozymes (Genes) in Tomato vegetative hypocotyls as influenced by auxin treatment. Our results indicate that ACC synthase is encoded by a multigene family and each gene is differentially induced by developmental, environmental and physiological factors.作者: Substitution 時間: 2025-3-30 13:03
Apple ACC Oxidase: Purification and Characterization of the Enzyme and Cloning of Its cDNA apple fruit was treated with ethylene, parallel increases in . ACC oxidase activity, . ACC oxidase activity and the level of pAE12 transcript were observed. These observations support the conclusion that the isolated ACC oxidase protein is encoded by pAE12.作者: 星星 時間: 2025-3-30 18:33
EFE Multigene Family in Tomato Plants: Expression and Characterizationession at the RNA level. Gene specific oligonucleotides were designed and used as primers in PCR reactions to amplify about 200 bp of the 3’ untranslated regions. The amplified fragments were cloned in a transcription vector generating appropriate probes for subsequent study of the expression of EFE作者: 袖章 時間: 2025-3-30 22:45 作者: negligence 時間: 2025-3-31 02:14 作者: Cougar 時間: 2025-3-31 05:55 作者: 他很靈活 時間: 2025-3-31 10:35
Betriebliche Gesundheitsf?rderungnes and then by demonstrating their functional expression in a heterologous host. These results have shed much light on the nature of this enigmatic enzyme including its cellular location and mechanism of action.作者: 過渡時期 時間: 2025-3-31 15:58
Patienten- und Angeh?rigenarbeitcomplex referred to as ethyleneforming-enzyme (EFE) (Yang and Hoffman,1984). The aims of the present work were to investigate the main characteristics and the regulation by calcium of EFE activity in protoplasts isolated from hypocotyls of sunflower (. L.) seedlings.作者: malign 時間: 2025-3-31 19:40
https://doi.org/10.1007/978-3-540-79333-5tson et al. poster 16, this meeting). This cDNA clone exhibits strong homology, both at the nucleotide and amino-acid levels, to the tomato clone pTOM 13, shown to express EFE in yeast (Hamilton et al. 1991).作者: Antarctic 時間: 2025-4-1 01:37
https://doi.org/10.1007/978-94-017-1003-9Expression; Fruit; Grove; Liana; Ornithin; Pathogen; Terpene; Transport; Xylem; evolution; gene expression; mor作者: 敲詐 時間: 2025-4-1 05:16 作者: CANE 時間: 2025-4-1 07:29 作者: 冒失 時間: 2025-4-1 14:00
Entbürokratisierte Pflegeplanunggenerated in tissues. Auxin did not induce the expression of the wound-inducible gene, and wounding did not express the auxin-inducible gene. The primary structures deduced from cDNAs for ACC synthases from different sources were compared in their sequence similarity. The overall similarity between 作者: 制造 時間: 2025-4-1 16:20
Entbürokratisierte Pflegeplanungato and winter squash ACC synthase, only the enzyme from tomato fruit tissue existed in a monomeric form, whereas the others in a dimeric form. The monomeric tomato ACC synthase was much less sensitive to the mechanism-based inactivation than the dimeric forms of ACC synthase. We suggest that ACC sy作者: 修改 時間: 2025-4-1 18:32 作者: GEON 時間: 2025-4-2 02:16 作者: Abduct 時間: 2025-4-2 03:09
Rund um die Uhr — Zeitgestaltunggel blotting suggested the existence of an ACC synthase multigene family in .. The other members are distantly related to .. The existence of at least another gene was confirmed by the isolation of a cDNA (.) from a flower-specific cDNA library. Expression of the . gene was studied by reverse-transc作者: 推崇 時間: 2025-4-2 08:10
Rund um die Uhr — Zeitgestaltungthe fruit is left on the tree to ripen, the more flavor is developed but, the longer the fruit is left on the tree less shelf-life of the fruit remains. A series of experiments with peach fruit were undertaken in order to understand the relationship of ethylene to ACC synthase RNA and EFE RNA and th作者: motivate 時間: 2025-4-2 11:57
Entbürokratisierte Pflegeplanungo loss of activity when the enzyme is stored at 0°C for 24 hr. When enzyme preparations from melon are desalted by passage through a column of Sephadex, activity is reduced. The lost activity can be restored by the inclusion of dilute NaOH to the reaction mixture, which gives a 6-fold stimulation of
