標題: Titlebook: Cellular Senescence; Methods and Protocol Marco Demaria Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 senesc [打印本頁] 作者: 轉變 時間: 2025-3-21 17:55
書目名稱Cellular Senescence影響因子(影響力)
書目名稱Cellular Senescence影響因子(影響力)學科排名
書目名稱Cellular Senescence網(wǎng)絡公開度
書目名稱Cellular Senescence網(wǎng)絡公開度學科排名
書目名稱Cellular Senescence被引頻次
書目名稱Cellular Senescence被引頻次學科排名
書目名稱Cellular Senescence年度引用
書目名稱Cellular Senescence年度引用學科排名
書目名稱Cellular Senescence讀者反饋
書目名稱Cellular Senescence讀者反饋學科排名
作者: ALB 時間: 2025-3-22 00:00 作者: Conjuction 時間: 2025-3-22 01:46
Tim M. Curtis Ph.D.,Tom A. Gardiner Ph.D. cells (Dimri et al., Proc Natl Acad Sci U S A 92:9363–9367, 1995). These cells, characterized by a permanent cell-cycle arrest (Hayflick and Moorhead, Exp Cell Res 25:585–621, 1961) and the production of a distinct secretory phenotype of cytokines, chemokines, and proteases (Coppe et al., PLoS Biol作者: VOC 時間: 2025-3-22 08:27
Low-Coherence Interference Microscopy The method has been developed by Cawthon in 2002 (Cawthon, Nucleic Acids Res 30:47e–47, 2002) and remains the most frequently used technique either in original or modified version. Telomere length is estimated by comparing the amount of telomere repeat amplification product (T) to a single copy gen作者: Audiometry 時間: 2025-3-22 09:32
Low-Coherence Interference Microscopyted with age-related pathologies such as cancer progression. Numerous functions of senescent cells depend on their ability to secrete bioactive molecules, a characteristic termed the senescence-associated secretory phenotype (SASP). Although the SASP is generally described as proinflammatory, its tr作者: Nonflammable 時間: 2025-3-22 14:47
Superresolution in Scanning Optical Systems. In oncogene-induced senescence, inflammasomes play a crucial role by regulating IL1R signaling and consequently modulating proliferation and the senescence-associated secretory phenotype (SASP). Inflammasome activation requires two steps: (a) priming of the inflammasome by activation of . expressi作者: Nonflammable 時間: 2025-3-22 19:49 作者: magnanimity 時間: 2025-3-22 22:45
Superresolution in Scanning Optical Systems suppressor mechanism limiting cancer progression. Here we describe IMR90 ER:RAS, a widely used model to study OIS in cell culture. This model takes advantage of IMR90 human primary fibroblast infected with a 4-hydroxy-tamoxifen (4-OHT) inducible ER:RAS construct. RAS activation upon 4-OHT treatment作者: Collision 時間: 2025-3-23 05:22 作者: brassy 時間: 2025-3-23 08:24 作者: Gossamer 時間: 2025-3-23 11:51
https://doi.org/10.1007/978-3-540-69565-3olic imbalance associated with aging and age-related diseases. Indications of a soluble state of lipofuscin have also been provided, rendering the perspective of monitoring such processes via lipofuscin quantification in liquids intriguing. Therefore, the development of an accurate and reliable meth作者: Dorsal 時間: 2025-3-23 17:09
Superresolution in Scanning Optical Systemse. Steady state levels of multiple metabolites change with senescence, and can be detected using analytical methods. Here, we describe a liquid chromatography–mass spectrometry (LC-MS) method for detecting altered metabolites from cultured senescent cells.作者: Merited 時間: 2025-3-23 19:06
Optical Trapping of Small Particlesvariety of stress conditions. Additionally, it plays a variety of physiological and pathophysiological roles in maintaining cell homeostasis. Recently, the critical role of autophagy during cellular senescence has been supported by evidences demonstrating the reversal of senescence by the reestablis作者: stress-test 時間: 2025-3-23 22:51 作者: 黃瓜 時間: 2025-3-24 05:03
Optical Trapping of Small Particlesrtions and deletions that are less than 10?bp in length. These limitations are largely due to challenges in accurately mapping short sequencing reads that significantly diverge from the reference genome. Newer sequencing-based methods have been developed to define and characterize larger DNA structu作者: 基因組 時間: 2025-3-24 10:16 作者: 兵團 時間: 2025-3-24 12:35 作者: 大廳 時間: 2025-3-24 17:14
https://doi.org/10.1007/978-1-4939-8931-7senescence phenotype; Reactive Oxygen Species; DNA damage; Senescence-Associated Secretory Phenotype; On作者: Console 時間: 2025-3-24 20:05
Springer Science+Business Media, LLC, part of Springer Nature 2019作者: DEFT 時間: 2025-3-24 23:14
Measurement of Metabolite Changes in Senescent Cells by Mass Spectrometry,e. Steady state levels of multiple metabolites change with senescence, and can be detected using analytical methods. Here, we describe a liquid chromatography–mass spectrometry (LC-MS) method for detecting altered metabolites from cultured senescent cells.作者: LARK 時間: 2025-3-25 06:08
Endothelial and Adrenergic ControlCellular senescence has been suggested to facilitate tissue regeneration via promoting cellular plasticity. Here, we describe multiple systems, both in vitro and in vivo, to detect senescence in the context of cellular reprogramming.作者: 單獨 時間: 2025-3-25 10:00 作者: Acetabulum 時間: 2025-3-25 13:05
Marco DemariaIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: paleolithic 時間: 2025-3-25 17:33 作者: 施魔法 時間: 2025-3-25 20:57
Cellular Senescence978-1-4939-8931-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 責問 時間: 2025-3-26 03:30
Superresolution in Scanning Optical Systemse. Steady state levels of multiple metabolites change with senescence, and can be detected using analytical methods. Here, we describe a liquid chromatography–mass spectrometry (LC-MS) method for detecting altered metabolites from cultured senescent cells.作者: 浮雕 時間: 2025-3-26 05:29
Reactive Oxygen Species Detection in Senescent Cells,ing molecules during senescence, stabilizing the cell-cycle arrest. In this chapter, we present a detailed description of protocols that allow us to characterize intracellular and extracellular ROS in live senescent cells.作者: 驚奇 時間: 2025-3-26 09:31
,Cellular Identification and Quantification of Senescence-Associated β-Galactosidase Activity In Viv 6:2853–2868, 2008), have received much attention in recent years for their impacts on diverse biological processes. Here we describe a method to identify and quantify the specific cells that become senescent in vivo using transmission electron microscopy after SA-β-gal staining that can be used in countless scenarios.作者: helper-T-cells 時間: 2025-3-26 14:37
IMR90 ER:RAS: A Cell Model of Oncogene-Induced Senescence, results in a coordinated induction of senescence, recapitulating different aspects of the phenotype such as the growth arrest and the establishment of a senescence-associated secretory phenotype (SASP).作者: 津貼 時間: 2025-3-26 19:48
1064-3745 ation advice from the experts.This book describes current methods for the identification and characterization of the major hallmarks of senescent cells. Chapters focus on the high heterogeneity of the senescence phenotypes, and techniques to induce and identify specific senescence programs. Addition作者: ITCH 時間: 2025-3-26 21:22
Superresolution in Scanning Optical Systemsescence-associated secretory phenotype (SASP). Inflammasome activation requires two steps: (a) priming of the inflammasome by activation of . expression, followed by (b) cleavage and release of mature IL-1β. In this chapter, we describe methods to detect both stages of inflammasome activation in cellular senescence.作者: 牽連 時間: 2025-3-27 04:15 作者: visceral-fat 時間: 2025-3-27 05:18
Measuring the Inflammasome in Oncogene-Induced Senescence,escence-associated secretory phenotype (SASP). Inflammasome activation requires two steps: (a) priming of the inflammasome by activation of . expression, followed by (b) cleavage and release of mature IL-1β. In this chapter, we describe methods to detect both stages of inflammasome activation in cellular senescence.作者: 心胸狹窄 時間: 2025-3-27 13:24 作者: 柏樹 時間: 2025-3-27 15:21
Neural Control of Ocular Blood Flowing molecules during senescence, stabilizing the cell-cycle arrest. In this chapter, we present a detailed description of protocols that allow us to characterize intracellular and extracellular ROS in live senescent cells.作者: 演講 時間: 2025-3-27 18:24
Tim M. Curtis Ph.D.,Tom A. Gardiner Ph.D. 6:2853–2868, 2008), have received much attention in recent years for their impacts on diverse biological processes. Here we describe a method to identify and quantify the specific cells that become senescent in vivo using transmission electron microscopy after SA-β-gal staining that can be used in countless scenarios.作者: Freeze 時間: 2025-3-28 01:52
Superresolution in Scanning Optical Systems results in a coordinated induction of senescence, recapitulating different aspects of the phenotype such as the growth arrest and the establishment of a senescence-associated secretory phenotype (SASP).作者: carotenoids 時間: 2025-3-28 04:46
Book 2019 heterogeneity of the senescence phenotypes, and techniques to induce and identify specific senescence programs. Additional chapters describe cellular and mouse models in which is possible to study the complex cell and non-cell autonomous functions of senescent cells.? Written in the highly successf作者: 約會 時間: 2025-3-28 08:02 作者: 是限制 時間: 2025-3-28 11:42
https://doi.org/10.1007/978-3-540-69565-3 our method allows for acquisition of reliable high-resolution single-cell copy number profiles. Moreover, the protocol allows multiplexing of 384 single-cell libraries in one sequencing run, thereby significantly reducing sequencing costs and can be completed in 3–4?days starting from single cell isolation to analysis of sequencing data.作者: ICLE 時間: 2025-3-28 16:57 作者: poliosis 時間: 2025-3-28 19:23
DNA Damage In Situ Ligation Followed by Proximity Ligation Assay (DI-PLA),sensitive detection of physical DSBs in fixed cells, through direct labeling of the DSBs with biotinylated oligonucleotides, and subsequent signal amplification by PLA between biotin and a partner protein in the proximity of the DNA break.作者: phase-2-enzyme 時間: 2025-3-29 00:24 作者: Urea508 時間: 2025-3-29 04:39
Mouse Models of Accelerated Cellular Senescence,n vivo remains challenging, as there is no marker unique to senescent cells. Here, we describe multiple methods to detect the presence and extent of cellular senescence in preclinical models, with a special emphasis on murine models of accelerated aging that exhibit a more rapid onset of cellular senescence.作者: 物質 時間: 2025-3-29 10:24
Book 2019nts, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...?Authoritative and cutting-edge, .Cellular Senescence: Methods and Protocols .aims to ensure successful results in the further study of this vital field..作者: 打擊 時間: 2025-3-29 14:17 作者: 紋章 時間: 2025-3-29 17:56
A Novel Quantitative Method for the Detection of Lipofuscin, the Main By-Product of Cellular Senescpreviously described assay, developed for in vitro and in vivo senescent cell recognition that exploits a newly synthesized Sudan Black-B analog (GL13). Analysis of human clinical samples with the modified protocol provided strong evidence of its usefulness for the exposure and surveillance of age-related conditions.作者: 賭博 時間: 2025-3-29 22:39 作者: FOR 時間: 2025-3-30 03:55
Reactive Oxygen Species Detection in Senescent Cells,ment and ageing. Senescent cells are characterized by increased production of reactive oxygen species (ROS), mostly produced by dysfunctional mitochondria. Both intracellular and extracellular ROS have been shown to contribute to the induction of senescence. ROS have also been shown to act as signal作者: 閑蕩 時間: 2025-3-30 05:33
,Cellular Identification and Quantification of Senescence-Associated β-Galactosidase Activity In Viv cells (Dimri et al., Proc Natl Acad Sci U S A 92:9363–9367, 1995). These cells, characterized by a permanent cell-cycle arrest (Hayflick and Moorhead, Exp Cell Res 25:585–621, 1961) and the production of a distinct secretory phenotype of cytokines, chemokines, and proteases (Coppe et al., PLoS Biol作者: Pcos971 時間: 2025-3-30 11:07
Relative Human Telomere Length Quantification by Real-Time PCR, The method has been developed by Cawthon in 2002 (Cawthon, Nucleic Acids Res 30:47e–47, 2002) and remains the most frequently used technique either in original or modified version. Telomere length is estimated by comparing the amount of telomere repeat amplification product (T) to a single copy gen作者: Gnrh670 時間: 2025-3-30 12:29
Assessing Functional Roles of the Senescence-Associated Secretory Phenotype (SASP),ted with age-related pathologies such as cancer progression. Numerous functions of senescent cells depend on their ability to secrete bioactive molecules, a characteristic termed the senescence-associated secretory phenotype (SASP). Although the SASP is generally described as proinflammatory, its tr作者: SNEER 時間: 2025-3-30 17:04 作者: 即席演說 時間: 2025-3-30 21:34 作者: TRAWL 時間: 2025-3-31 01:47
IMR90 ER:RAS: A Cell Model of Oncogene-Induced Senescence, suppressor mechanism limiting cancer progression. Here we describe IMR90 ER:RAS, a widely used model to study OIS in cell culture. This model takes advantage of IMR90 human primary fibroblast infected with a 4-hydroxy-tamoxifen (4-OHT) inducible ER:RAS construct. RAS activation upon 4-OHT treatment作者: 沖突 時間: 2025-3-31 07:12
Genotoxic Stress-Induced Senescence,hemotherapy, apply. If the DNA repair machinery fails to fix the damaged site during a temporary cell-cycle arrest, or if massive genotoxic stress overwhelmed the repair capacity, cellular failsafe programs such as apoptosis or senescence will be triggered to limit aberrant propagation of these dama作者: CUB 時間: 2025-3-31 10:03
A Multiparametric Assay to Evaluate Senescent Cells,with age and are present at sites of tissue damage and age related pathologies. However, the characterization of senescence cells in vivo is currently limited and the need for new technologies to detect and monitor the senescence state in vivo has greatly increased. Here we demonstrate the use of th作者: cultivated 時間: 2025-3-31 15:03 作者: Fluctuate 時間: 2025-3-31 19:02
Measurement of Metabolite Changes in Senescent Cells by Mass Spectrometry,e. Steady state levels of multiple metabolites change with senescence, and can be detected using analytical methods. Here, we describe a liquid chromatography–mass spectrometry (LC-MS) method for detecting altered metabolites from cultured senescent cells.作者: 下垂 時間: 2025-3-31 23:18 作者: Obstreperous 時間: 2025-4-1 02:58 作者: 上下連貫 時間: 2025-4-1 09:21 作者: sorbitol 時間: 2025-4-1 12:00
Mouse Models of Accelerated Cellular Senescence,ellular stress intended to block the propagation of damaged cells. Senescent cells have been demonstrated to play a causal role in aging via their senescence-associated secretory phenotype and by impeding tissue regeneration. Depletion of senescent cells either through genetic or pharmacologic metho作者: incite 時間: 2025-4-1 18:14
