標(biāo)題: Titlebook: Cellular Imaging; Electron Tomography Eric Hanssen Book 2018 Springer International Publishing AG 2018 3D Reconstruction of Images.Analysi [打印本頁(yè)] 作者: 出租 時(shí)間: 2025-3-21 17:02
書目名稱Cellular Imaging影響因子(影響力)
作者: aesthetic 時(shí)間: 2025-3-22 00:15 作者: SEEK 時(shí)間: 2025-3-22 01:10 作者: Ethics 時(shí)間: 2025-3-22 08:07 作者: unstable-angina 時(shí)間: 2025-3-22 12:10 作者: ATRIA 時(shí)間: 2025-3-22 14:05
,Conjugate Immunofluorescence—SEM Array Tomography for Studying Mammalian Synapses and Axons,ased on physical ultrathin serial sectioning, immunostaining and acquiring fluorescence and electron microscopy images of resin embedded tissues, followed by computational volume reconstruction and analysis. Conjugate immunofluorescence—SEM array tomography has been used for the study of brain tissu作者: ATRIA 時(shí)間: 2025-3-22 20:21
Alignment of Tilt Series,ection alignment, and 3D reconstruction. In this chapter we will focus on the process of alignment moving from the justification of its need to the study of the different classical approaches (cross-correlation, use of added fiducial markers) that have been commonly used in this alignment process. W作者: 男生戴手銬 時(shí)間: 2025-3-22 22:46 作者: Onerous 時(shí)間: 2025-3-23 03:11
Resolution in Electron Tomography, in the nanometre range. In this chapter I qualitatively discuss the typical limitations that occur in electron tomography of biological samples depending on the imaging modalities, with the focus on cryo electron tomography and subtomogram averaging.作者: concise 時(shí)間: 2025-3-23 07:53
Signal Optimization in Electron Tomography,timization may be achieved through image processing algorithms that minimize noise and artefacts in either raw or reconstructed image signal. Procedures for signal optimization may be performed in real or Fourier space. We survey procedures and applications with an emphasis on cryotomography of froz作者: 裝入膠囊 時(shí)間: 2025-3-23 11:19 作者: DUCE 時(shí)間: 2025-3-23 15:03
Organisationsentwicklung und Fachlichkeitcted using ordinary montaging to?~10?×?10?μm. The desire to study areas larger than this has spurred the development of methods for stitching together laterally adjacent tomograms, referred to as super-montaging. In this chapter, we describe the steps involved in performing large-scale tomography in作者: Morsel 時(shí)間: 2025-3-23 21:43 作者: 情感 時(shí)間: 2025-3-23 22:39
https://doi.org/10.1007/978-3-531-91117-5Cryo-electron tomography (CET) provides three-dimensional (3D) views on the structure and organization of a wide range of frozen-hydrated specimens under near-to-native conditions. In combination with advanced image processing methods, the locations, orientations and structures of large macromolecular complexes can be mined from tomographic data.作者: GLADE 時(shí)間: 2025-3-24 04:23
Structural Biology in Situ Using Cryo-Electron Subtomogram Analysis,Cryo-electron tomography (CET) provides three-dimensional (3D) views on the structure and organization of a wide range of frozen-hydrated specimens under near-to-native conditions. In combination with advanced image processing methods, the locations, orientations and structures of large macromolecular complexes can be mined from tomographic data.作者: 發(fā)展 時(shí)間: 2025-3-24 09:33 作者: 羽毛長(zhǎng)成 時(shí)間: 2025-3-24 13:29
https://doi.org/10.1007/978-3-531-90943-1ned samples, and for vitrified, unstained cells. With the proliferation of modern TEM microscopes that allow for switching between TEM and STEM modes with relative ease, we expect the use of STEM tomography to increase. The concepts for STEM imaging are significantly different than for TEM, and ther作者: WITH 時(shí)間: 2025-3-24 15:32
Organisationsentwicklung und Fachlichkeitn uniquely provide mechanistic insights into in situ biology at molecular and cellular scales. To produce these images, specimens are?preserved by flash-freezing without stains or fixatives; these flash-frozen specimens are imaged over a range of angles in an electron microscope and this data used t作者: 流行 時(shí)間: 2025-3-24 19:03 作者: Feedback 時(shí)間: 2025-3-25 01:02
https://doi.org/10.1007/978-3-531-90943-1 main contributors to this are the Serial Block-Face SEM (SBEM) and Focused Ion Beam SEM (FIB-SEM). The resolution offered by the FIB-SEM makes it the go-to tool for studies at higher resolution over smaller areas, particularly for subcellular studies. Compared to FIB-SEM, SBEM gives a much larger f作者: 外露 時(shí)間: 2025-3-25 05:04 作者: 管理員 時(shí)間: 2025-3-25 07:41
Organisationsentwicklung und Fachlichkeitection alignment, and 3D reconstruction. In this chapter we will focus on the process of alignment moving from the justification of its need to the study of the different classical approaches (cross-correlation, use of added fiducial markers) that have been commonly used in this alignment process. W作者: 等級(jí)的上升 時(shí)間: 2025-3-25 11:42
Innovation und Besch?ftigungsstabilit?t that the 3D information can be recovered by “back-projecting” all the images in the correct geometry. We use algorithms that are integrative (back-projection or Fourier inversion) or iterative (algebraic or maximum entropy methods). In practical tomography, we can only record a finite set of images作者: bacteria 時(shí)間: 2025-3-25 16:31 作者: Irksome 時(shí)間: 2025-3-25 22:02 作者: Euthyroid 時(shí)間: 2025-3-26 01:48
Astrid Seltrecht Dipl. P?d., Dr.owever, owing to the complexity and low signal-to-noise ratio of the reconstructions, direct interpretation of their three-dimensional content is not straight forward. This chapter describes computational approaches designed to extract the most accurate information possible from biological electron 作者: Anhydrous 時(shí)間: 2025-3-26 05:15
https://doi.org/10.1007/978-3-319-68997-53D Reconstruction of Images; Analysis of Cell Structure; Array Tomography; Block Face Tomography; Electr作者: somnambulism 時(shí)間: 2025-3-26 09:10 作者: Apogee 時(shí)間: 2025-3-26 16:40 作者: 生意行為 時(shí)間: 2025-3-26 18:42
Organisationsentwicklung und Fachlichkeitfficiency of descriptive studies of morphology, and in the case of structural biology, to enable the transition from a reductionist approach to in-depth systems biology. The chapter concludes with a step-by-step guide to acquiring tomograms.作者: 熄滅 時(shí)間: 2025-3-27 00:49 作者: 動(dòng)機(jī) 時(shí)間: 2025-3-27 03:58
Astrid Seltrecht Dipl. P?d., Dr.timization may be achieved through image processing algorithms that minimize noise and artefacts in either raw or reconstructed image signal. Procedures for signal optimization may be performed in real or Fourier space. We survey procedures and applications with an emphasis on cryotomography of frozen-hydrated biological specimens.作者: Obsessed 時(shí)間: 2025-3-27 05:33
Astrid Seltrecht Dipl. P?d., Dr.owever, owing to the complexity and low signal-to-noise ratio of the reconstructions, direct interpretation of their three-dimensional content is not straight forward. This chapter describes computational approaches designed to extract the most accurate information possible from biological electron tomograms.作者: hemorrhage 時(shí)間: 2025-3-27 10:23 作者: STENT 時(shí)間: 2025-3-27 17:31
Biological and Medical Physics, Biomedical Engineeringhttp://image.papertrans.cn/c/image/223032.jpg作者: 進(jìn)步 時(shí)間: 2025-3-27 20:16
Electron Tomography: A Primer,fficiency of descriptive studies of morphology, and in the case of structural biology, to enable the transition from a reductionist approach to in-depth systems biology. The chapter concludes with a step-by-step guide to acquiring tomograms.作者: Flatus 時(shí)間: 2025-3-28 01:13 作者: cleaver 時(shí)間: 2025-3-28 03:23 作者: 小蟲 時(shí)間: 2025-3-28 08:12
Segmentation of Features in Electron Tomographic Reconstructions,owever, owing to the complexity and low signal-to-noise ratio of the reconstructions, direct interpretation of their three-dimensional content is not straight forward. This chapter describes computational approaches designed to extract the most accurate information possible from biological electron tomograms.作者: 中古 時(shí)間: 2025-3-28 11:57
Book 2018n addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging).?.The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing t作者: 鍵琴 時(shí)間: 2025-3-28 18:22 作者: 荒唐 時(shí)間: 2025-3-28 19:42
1618-7210 transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation.978-3-319-88699-2978-3-319-68997-5Series ISSN 1618-7210 Series E-ISSN 2197-5647 作者: 楓樹(shù) 時(shí)間: 2025-3-29 02:19
Organisationsentwicklung und Fachlichkeito reconstruct 3-D images of the specimen in near-native state to ‘macromolecular’ resolution. In this introductory chapter we take the perspective of the workflow of a cryo-tomography project to outline the technique, and important underlying concepts.作者: DEFT 時(shí)間: 2025-3-29 06:59 作者: JOG 時(shí)間: 2025-3-29 10:33
Innovation und Besch?ftigungsstabilit?t at a dose low enough to avoid radiation damage, yielding noisy tomograms with missing information. The quality of the tomograms depends on the algorithmic details, but also on the pre-processing of the tilt series images, and post-processing of the tomographic volume.作者: 角斗士 時(shí)間: 2025-3-29 13:52 作者: CANE 時(shí)間: 2025-3-29 18:40
https://doi.org/10.1007/978-3-531-90943-1with relative ease, we expect the use of STEM tomography to increase. The concepts for STEM imaging are significantly different than for TEM, and therefore we will describe in detail the STEM imaging modality, followed by STEM tomography concepts and applications.作者: 善于 時(shí)間: 2025-3-29 20:19 作者: 單調(diào)性 時(shí)間: 2025-3-30 03:08 作者: BOOM 時(shí)間: 2025-3-30 04:39
,Conjugate Immunofluorescence—SEM Array Tomography for Studying Mammalian Synapses and Axons,owed by computational volume reconstruction and analysis. Conjugate immunofluorescence—SEM array tomography has been used for the study of brain tissue, and in particular for the characterization of diverse synapses and axons.
