標題: Titlebook: Cell-free DNA as Diagnostic Markers; Methods and Protocol Valentina Casadio,Samanta Salvi Book 2019 Springer Science+Business Media, LLC, p [打印本頁] 作者: digestive-tract 時間: 2025-3-21 19:15
書目名稱Cell-free DNA as Diagnostic Markers影響因子(影響力)
書目名稱Cell-free DNA as Diagnostic Markers影響因子(影響力)學科排名
書目名稱Cell-free DNA as Diagnostic Markers網(wǎng)絡公開度
書目名稱Cell-free DNA as Diagnostic Markers網(wǎng)絡公開度學科排名
書目名稱Cell-free DNA as Diagnostic Markers被引頻次
書目名稱Cell-free DNA as Diagnostic Markers被引頻次學科排名
書目名稱Cell-free DNA as Diagnostic Markers年度引用
書目名稱Cell-free DNA as Diagnostic Markers年度引用學科排名
書目名稱Cell-free DNA as Diagnostic Markers讀者反饋
書目名稱Cell-free DNA as Diagnostic Markers讀者反饋學科排名
作者: 圍巾 時間: 2025-3-21 22:23 作者: deciduous 時間: 2025-3-22 02:00 作者: 驕傲 時間: 2025-3-22 04:52 作者: 無彈性 時間: 2025-3-22 11:08
Cell-Free DNA Integrity: Applicationsen studied for cfDNAi, such as specific genes involved in cancer progression or repetitive DNA sequences as surrogate markers for the whole circulating DNA..However, the lack of a standardized method for cfDNAi evaluation remains one of its main critical issues. Apoptotic index (AI) and integrity in作者: panorama 時間: 2025-3-22 13:26 作者: panorama 時間: 2025-3-22 18:01
Studying Copy Number Variations in Cell-Free DNA: The Example of AR in Prostate Cancerrting from the hypothesis that the gain of androgen receptor (.) gene is a frequent aberration in advanced prostate cancer patients, we analyzed it in cfDNA as a potential predictive biomarker of specific treatments. Here we report a general protocol that may be considered to analyze gene copy numbe作者: 胰臟 時間: 2025-3-22 22:37 作者: 飛來飛去真休 時間: 2025-3-23 04:49
From cfDNA to Sequencing: Workflows and Potentialsesistance by following the mutation status changes of patients. In this field, high-sensitivity methods like next-generation sequencing (NGS) could help to accurately detect somatic mutations at low frequency. Here, we report some advantages and limitations of NGS approaches for cfDNA mutation analy作者: LUDE 時間: 2025-3-23 06:24 作者: 者變 時間: 2025-3-23 10:57
Quantitative Methylation-Specific PCR: A Simple Method for Studying Epigenetic Modifications of Cellnd prediction of therapeutic response. Quantification of cfDNA methylation levels requires methods with high sensitivity and specificity due to low amounts of cfDNA available in plasma, high degradation of cfDNA, and/or contamination with genomic DNA. To date, several approaches for measuring cfDNA 作者: prostate-gland 時間: 2025-3-23 14:50 作者: fiction 時間: 2025-3-23 18:39 作者: 昆蟲 時間: 2025-3-24 02:16
Book 2019liquid biopsy for cancer disease, methods in cancer, epigenetic modifications, fetal and pediatric diseases, physical activity, and urinary cell free DNA. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists o作者: Outspoken 時間: 2025-3-24 02:26
Cell-Free DNA Integrity: Applicationsdex (II) of cfDNA could be useful biomarkers to identify the patients likely to recur, progress, or relapse, which is an urgent need in translational research in oncology..Here it is provided an adequate method of cfDNAi analysis: a cheap and reproducible tool, robust and performable in all laboratories using a real-time PCR instrument.作者: debacle 時間: 2025-3-24 07:09
Book 2019f the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..?Authoritative and cutting-edge, .Cell-Free DNA as Diagnostic Markers: Methods and Protocols .aims to ensure successful results in the further study of this vital field..作者: 友好 時間: 2025-3-24 11:21
Osteoporosis Screening and Diagnosis,dex (II) of cfDNA could be useful biomarkers to identify the patients likely to recur, progress, or relapse, which is an urgent need in translational research in oncology..Here it is provided an adequate method of cfDNAi analysis: a cheap and reproducible tool, robust and performable in all laboratories using a real-time PCR instrument.作者: ectropion 時間: 2025-3-24 15:37
1064-3745 ation advice from the experts.This book describes the most important techniques used for studying cfDNA in the different samples; serum, plasma, urine. Chapters detail methods on liquid biopsy for cancer disease, methods in cancer, epigenetic modifications, fetal and pediatric diseases, physical act作者: ELATE 時間: 2025-3-24 19:06 作者: CLAMP 時間: 2025-3-25 01:30 作者: transient-pain 時間: 2025-3-25 06:19 作者: 現(xiàn)存 時間: 2025-3-25 11:03 作者: Subjugate 時間: 2025-3-25 13:42
From cfDNA to Sequencing: Workflows and Potentialslp to accurately detect somatic mutations at low frequency. Here, we report some advantages and limitations of NGS approaches for cfDNA mutation analyses with the aim of choosing the most suitable in terms of sensitivity, specificity, data output, costs, and time work.作者: 表兩個 時間: 2025-3-25 15:48 作者: outskirts 時間: 2025-3-25 21:24
Observer-Based Fault Estimation Techniquesnitoring, clinical decision, and patients’ outcome..cfDNA has already given a huge impact on prenatal medicine, and it could become, in the next future, the standard of care also in other fields, from oncology to transplant medicine and cardiovascular diseases.作者: prick-test 時間: 2025-3-26 00:32 作者: Crohns-disease 時間: 2025-3-26 05:05 作者: angiography 時間: 2025-3-26 11:24
Foteini Cheirdari,George Karabatise consists of a pair of primers and two probes labeled with a fluorescent dye capable to recognize the presence or absence of a specific mutation. Using the dPCR method, we can identify only a few mutations in tissue that are present also in plasma.作者: Fibrinogen 時間: 2025-3-26 13:27 作者: restrain 時間: 2025-3-26 17:03
Cell-Free DNA: Applications in Different Diseasesnitoring, clinical decision, and patients’ outcome..cfDNA has already given a huge impact on prenatal medicine, and it could become, in the next future, the standard of care also in other fields, from oncology to transplant medicine and cardiovascular diseases.作者: Prophylaxis 時間: 2025-3-26 21:09
Circulating Cell-Free DNA and Cancer Therapy Monitoring: Methods and Potentialresence of possible metastasis, (4) a marker of the success of secondary subsequent treatment, (5) determining which patients can benefit from a particular treatment, and (6) offering a prognosis. These aspects will be discussed concerning the application of circulating tumor DNA analysis to the monitoring of cancer patients undergoing therapy.作者: 憤慨一下 時間: 2025-3-27 04:37
Copy Number Variation Analysis on Cell-Free Serum DNANVs in circulating, cell-free DNA by multiplex ligation-dependent probe amplification (MLPA). MLPA represents an efficient method for the detection of CNVs among numerous genes on various chromosomal regions in serum.作者: Promotion 時間: 2025-3-27 07:21
dPCR Mutational Analyses in Cell-Free DNA: A Comparison with Tissuese consists of a pair of primers and two probes labeled with a fluorescent dye capable to recognize the presence or absence of a specific mutation. Using the dPCR method, we can identify only a few mutations in tissue that are present also in plasma.作者: macular-edema 時間: 2025-3-27 13:15
Quantitative Methylation-Specific PCR: A Simple Method for Studying Epigenetic Modifications of Cellue that can be easily implemented into clinical practice. In this chapter, we provide a detailed protocol for SYBR Green qMSP analysis which is currently used in our laboratory for cfDNA methylation detection. Useful information regarding successful qMSP primers design are also provided.作者: 名字的誤用 時間: 2025-3-27 17:36
Studying Copy Number Variations in Cell-Free DNA: The Example of AR in Prostate Cancerrting from the hypothesis that the gain of androgen receptor (.) gene is a frequent aberration in advanced prostate cancer patients, we analyzed it in cfDNA as a potential predictive biomarker of specific treatments. Here we report a general protocol that may be considered to analyze gene copy number variations in serum or plasma fluids.作者: 傳染 時間: 2025-3-27 19:41 作者: 破裂 時間: 2025-3-28 00:09
Observer-Based Fault Estimation Techniquesasive disease biomarker. The interest in clinical applications has gained an exponential increase, making it a popular and potential target in a wide range of research areas..cfDNA can be found in different body fluids, both in healthy and not healthy subjects. The recent and rapid development of ne作者: ILEUM 時間: 2025-3-28 02:32
Christian Müller-Schloer,Sven Tomfordeimportant to optimize pre-analytical and analytical procedures in order to maximize the performance of cfDNA-based analyses..In this chapter, we report the most common methods for the correct collection, centrifugation, storage, and DNA isolation from cell-free biological sources such as plasma, uri作者: reserve 時間: 2025-3-28 09:43
https://doi.org/10.1007/978-3-319-68938-8either approach has the sensitivity or specificity to be very sure of the efficacy of the treatment. Moreover, PET/CT scans can be both comparatively expensive and produce low levels of radiation for the patient. The advent of the possibility of using circulating DNA released from the tumor permits 作者: 召集 時間: 2025-3-28 11:04
Oaks and People: A Long Journey Together,ch show a source of potential biomarkers. In cancer patients, it is a simple and less invasive mean, representing a sustainable alternative to interrogate all tumor cells longitudinally, quantifying and characterizing the biological materials (DNAs, RNAs, proteins) which originate from cancer tissue作者: Influx 時間: 2025-3-28 16:44 作者: Perceive 時間: 2025-3-28 20:22 作者: Osteoporosis 時間: 2025-3-29 00:53
Organised Crime Groups involved in Fraudrting from the hypothesis that the gain of androgen receptor (.) gene is a frequent aberration in advanced prostate cancer patients, we analyzed it in cfDNA as a potential predictive biomarker of specific treatments. Here we report a general protocol that may be considered to analyze gene copy numbe作者: 我怕被刺穿 時間: 2025-3-29 06:36
Foteini Cheirdari,George Karabatisnalysis of mutations in tumor tissue DNA and plasma cell-free DNA (cfDNA) using a dPCR method. Tumor cells are captured by laser microdissection to obtain only cancerous cells from a small quantity of metastatic tissue samples and to exclude inflammatory and stromal cells. We extracted cfDNA from 50作者: Medley 時間: 2025-3-29 10:36 作者: candle 時間: 2025-3-29 14:22
Sankalita Mandal,Marcin Hewelt,Mathias Wesken DNA sequence. The mainly studied epigenetic alteration is DNA methylation occurring at CpG islands in the promoter regions for which several literature data showed clinical relevance. This chapter is an overview of the epigenetic alterations detected in cell-free DNA.作者: 事與愿違 時間: 2025-3-29 18:14
Sankalita Mandal,Marcin Hewelt,Mathias Weskend prediction of therapeutic response. Quantification of cfDNA methylation levels requires methods with high sensitivity and specificity due to low amounts of cfDNA available in plasma, high degradation of cfDNA, and/or contamination with genomic DNA. To date, several approaches for measuring cfDNA 作者: 錫箔紙 時間: 2025-3-29 19:52
https://doi.org/10.1007/978-3-319-69505-1tor. Chances of an effective cure in childhood cancer rely on the capacity to make an early and accurate diagnosis, detect metastatic disease or relapse, and predict the response to treatment..Liquid biopsy is a very promising blood test for cancer detection and noninvasive disease monitoring. This 作者: 膽小鬼 時間: 2025-3-30 01:21
Lisa E. Flaum,William J. Gradishar way into global clinical applications. The fractional concentration of cell-free fetal DNA in plasma DNA of a pregnant woman is an important parameter for understanding and interpreting analytical results of NIPT. Thus, the accurate quantification of fetal DNA fraction is indispensable when NIPT is作者: concentrate 時間: 2025-3-30 07:11 作者: MEN 時間: 2025-3-30 09:53
Springer Science+Business Media, LLC, part of Springer Nature 2019作者: VEIL 時間: 2025-3-30 15:49
Valentina Casadio,Samanta SalviIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: Matrimony 時間: 2025-3-30 19:53
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/222955.jpg
