標(biāo)題: Titlebook: Cell-Wide Identification of Metabolite-Protein Interactions; Aleksandra Skirycz,Marcin Luzarowski,Jennifer C. E Book 2023 The Editor(s) (i [打印本頁] 作者: 小天使 時(shí)間: 2025-3-21 18:41
書目名稱Cell-Wide Identification of Metabolite-Protein Interactions影響因子(影響力)
書目名稱Cell-Wide Identification of Metabolite-Protein Interactions影響因子(影響力)學(xué)科排名
書目名稱Cell-Wide Identification of Metabolite-Protein Interactions網(wǎng)絡(luò)公開度
書目名稱Cell-Wide Identification of Metabolite-Protein Interactions網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cell-Wide Identification of Metabolite-Protein Interactions被引頻次
書目名稱Cell-Wide Identification of Metabolite-Protein Interactions被引頻次學(xué)科排名
書目名稱Cell-Wide Identification of Metabolite-Protein Interactions年度引用
書目名稱Cell-Wide Identification of Metabolite-Protein Interactions年度引用學(xué)科排名
書目名稱Cell-Wide Identification of Metabolite-Protein Interactions讀者反饋
書目名稱Cell-Wide Identification of Metabolite-Protein Interactions讀者反饋學(xué)科排名
作者: 積極詞匯 時(shí)間: 2025-3-21 22:38 作者: FIR 時(shí)間: 2025-3-22 00:38
Land Use Regulations and Fertility Rateslly uncovering the complexity of the protein–metabolite interactome. Here, we highlight some classic and recent examples of how protein metabolite interactions regulate metabolism, both locally and globally, and how this contributes to cellular physiology. We also discuss the importance of these int作者: 混合 時(shí)間: 2025-3-22 08:07
https://doi.org/10.1007/978-3-319-66869-7nowledge is still limited. The screening of PMIs using small molecules as bait will broaden our ability to uncover novel PMIs, setting the basis for establishing their biological relevance. Here, we describe a protocol that allows the identification of multiple protein partners for one ligand. This 作者: 充足 時(shí)間: 2025-3-22 09:24
https://doi.org/10.1007/978-3-319-66920-5o classical genetics in plants, the identification of small-molecule targets remains a challenge and limits the broad use of the compounds. The cellular thermal shift assay (CETSA), based on the principle that binding of small molecules could affect the thermal stability of proteins, has been applie作者: 淺灘 時(shí)間: 2025-3-22 15:17 作者: 淺灘 時(shí)間: 2025-3-22 17:04
Application: Treatment Wetlands,techniques have emerged to identify and characterize protein–metabolite interactions. However, their implementation in plants is generally lagging behind, preventing a complete understanding of the regulatory mechanisms governing plant physiology. Recently, a novel approach to identify metabolite-bi作者: Factorable 時(shí)間: 2025-3-22 22:29 作者: GENUS 時(shí)間: 2025-3-23 03:39
Application: Nitrate Reduction Processes,get deconvolution of ligands, requiring no chemical ligand modification. PISA can be applied to living cells for studying target engagement in vivo or alternatively to protein extracts to identify in vitro ligand-interacting proteins. Here we describe the PISA workflow optimized in our lab. PISA imp作者: 執(zhí) 時(shí)間: 2025-3-23 06:15 作者: languid 時(shí)間: 2025-3-23 10:34
https://doi.org/10.1007/978-3-319-67308-0nding of small molecules, such as metabolites, to biological macromolecules like proteins and nucleic acids. STD NMR allows detection of binding in complex mixtures of potential ligands, which is often used for library screening in the pharmaceutical industry but may also be beneficial for binding s作者: 多嘴多舌 時(shí)間: 2025-3-23 14:58 作者: DAMP 時(shí)間: 2025-3-23 21:22 作者: 項(xiàng)目 時(shí)間: 2025-3-24 01:04
Data Science Meets Optimization, driven primarily by the needs of drug development. While, in principle, many of the computational methods developed in the context of drug development can also be applied directly to the investigation of metabolite-protein interactions, the interactions of metabolites with proteins (enzymes) are c作者: constellation 時(shí)間: 2025-3-24 03:15 作者: Introvert 時(shí)間: 2025-3-24 09:55 作者: 造反,叛亂 時(shí)間: 2025-3-24 10:51 作者: 粗鄙的人 時(shí)間: 2025-3-24 16:48
Land Use Regulations and Fertility Rateslly uncovering the complexity of the protein–metabolite interactome. Here, we highlight some classic and recent examples of how protein metabolite interactions regulate metabolism, both locally and globally, and how this contributes to cellular physiology. We also discuss the importance of these interactions in diseases such as cancer.作者: Hay-Fever 時(shí)間: 2025-3-24 22:42 作者: 破譯 時(shí)間: 2025-3-25 02:06 作者: Phagocytes 時(shí)間: 2025-3-25 05:02 作者: 南極 時(shí)間: 2025-3-25 09:53 作者: 媒介 時(shí)間: 2025-3-25 11:51
,PROMIS: Co-fractionation Mass Spectrometry for Analysis of Protein–Metabolite Interactions,—but a comprehensive overview of protein–metabolite interactions (PMIs) is missing. Recently we devised a non-targeted method for detecting PMIs using size-exclusion chromatography followed by proteomic and metabolomic analysis: PROMIS. Under test this method was able to identify known PMIs such as enzyme–cofactor complexes as well as novel ones.作者: 帽子 時(shí)間: 2025-3-25 16:03
Solvent-Induced Protein Precipitation for Drug Target Discovery,nce to solvent-induced precipitation. This chapter describes the protocol for identifying drug–target protein interactions by performing unbiased SIP with total cell lysate using a mass spectrometry-based proteomic strategy.作者: Flatus 時(shí)間: 2025-3-25 20:39
Databases and Tools to Investigate Protein-Metabolite Interactions, our knowledge on PMIs, with particular emphasis on platforms and databases to explore the highly heterogenous nature of characterized PMIs, which is likely to be an essential resource on the development of new computational approaches to predict and validate interactions based on large-scale PMI screenings.作者: 大范圍流行 時(shí)間: 2025-3-26 03:47
Book 2023erent organisms, in order to shed light on these important regulatory interactions. Experimental and computational strategies to characterize protein-metabolite interactions are discussed, and recent advances in enabling technologies are featured as well. Written for the highly successful .Methods i作者: gerrymander 時(shí)間: 2025-3-26 06:32
1064-3745 expertsThis thorough volume explores protocols of proteome- and metabolome-wide strategies for the identification of protein-small molecule complexes in different organisms, in order to shed light on these important regulatory interactions. Experimental and computational strategies to characterize 作者: 6Applepolish 時(shí)間: 2025-3-26 11:34
https://doi.org/10.1007/978-3-319-66869-7stablishing their biological relevance. Here, we describe a protocol that allows the identification of multiple protein partners for one ligand. This protocol describes a straightforward methodology that can be adapted to a wide variety of organisms.作者: Flirtatious 時(shí)間: 2025-3-26 12:52
Data Science Meets Optimizationffinity purification (AP) followed by liquid chromatography–mass spectrometry (LC–MS). Aside from its speed, simplicity, and adaptability to a variety of biological systems, its main strength lies in the parallel identification, in a near-physiological environment, of a given protein’s protein and small-molecule partners.作者: Headstrong 時(shí)間: 2025-3-26 17:22 作者: 險(xiǎn)代理人 時(shí)間: 2025-3-26 21:46 作者: 壓艙物 時(shí)間: 2025-3-27 01:25
https://doi.org/10.1007/978-3-319-66920-5nges in the protein thermal stability caused by the interactions with small molecules at the proteome level. Here we describe the small-molecule target validation as well as the target identification with mass spectrometry by means of CETSA.作者: 抱狗不敢前 時(shí)間: 2025-3-27 07:27 作者: ALIAS 時(shí)間: 2025-3-27 09:49
https://doi.org/10.1007/978-3-319-66920-5nce to solvent-induced precipitation. This chapter describes the protocol for identifying drug–target protein interactions by performing unbiased SIP with total cell lysate using a mass spectrometry-based proteomic strategy.作者: 露天歷史劇 時(shí)間: 2025-3-27 15:56 作者: evasive 時(shí)間: 2025-3-27 20:30
Book 2023 and practical, .Cell-Wide Identification of Metabolite-Protein Interactions. will aid researchers seeking a better understanding of the mechanisms of signal transduction occurring in the cell and assessing the effect of complex formation on cell physiology..作者: 煉油廠 時(shí)間: 2025-3-28 00:31 作者: optional 時(shí)間: 2025-3-28 02:54
Application: Nitrate Reduction Processes,e. PISA dramatically reduces analysis costs, allowing many research questions in need of target deconvolution to be addressed, and unlocks the potential of miniaturizing biological models, including primary cells.作者: 占卜者 時(shí)間: 2025-3-28 07:54 作者: Pastry 時(shí)間: 2025-3-28 10:58 作者: 使成核 時(shí)間: 2025-3-28 17:30
Proteome Integral Solubility Alteration (PISA) for High-Throughput Ligand Target Deconvolution withe. PISA dramatically reduces analysis costs, allowing many research questions in need of target deconvolution to be addressed, and unlocks the potential of miniaturizing biological models, including primary cells.作者: critic 時(shí)間: 2025-3-28 22:09
Biophysical Approaches for the Characterization of Protein-Metabolite Interactions,tate-of-the-art biophysical toolbox for the study of protein-metabolite interactions. It briefly introduces basic principles, highlights recent examples from the literature, and pinpoints promising future directions.作者: FATAL 時(shí)間: 2025-3-28 23:22
,Identification of Plant Protein–Metabolite Interactions by Limited Proteolysis-Coupled Mass Spectroparation is performed including a complete digestion with the sequence-specific protease trypsin. Finally, label-free proteomics analysis is applied to identify structure-dependent proteolytic patterns corresponding to protein targets of the specific metabolite and their binding sites. Given its ame作者: 我們的面粉 時(shí)間: 2025-3-29 03:38
Detecting and Characterizing Interactions of Metabolites with Proteins by Saturation Transfer Diffenteractions of natural products and metabolites, in particular. The reader is also referred to excellent reviews of the field and the literature cited therein. This chapter also provides a detailed experimental protocol for performing the STD NMR measurement based on the example of the subunit A of 作者: 使熄滅 時(shí)間: 2025-3-29 10:45 作者: 不成比例 時(shí)間: 2025-3-29 13:55 作者: indifferent 時(shí)間: 2025-3-29 15:46 作者: BLA 時(shí)間: 2025-3-29 22:48 作者: pessimism 時(shí)間: 2025-3-30 00:49 作者: 哎呦 時(shí)間: 2025-3-30 07:00
https://doi.org/10.1007/978-3-319-67308-0nteractions of natural products and metabolites, in particular. The reader is also referred to excellent reviews of the field and the literature cited therein. This chapter also provides a detailed experimental protocol for performing the STD NMR measurement based on the example of the subunit A of
