標(biāo)題: Titlebook: Cell-Based Microarrays; Methods and Protocol Peter Ertl,Mario Rothbauer Book 2018 Springer Science+Business Media, LLC, part of Springer Na [打印本頁] 作者: 加冕 時(shí)間: 2025-3-21 19:28
書目名稱Cell-Based Microarrays影響因子(影響力)
作者: Awning 時(shí)間: 2025-3-22 00:01
Beyond the Ideal Political Apologyive patterning of biomolecules on thiol–ene solid supports. We describe the preparation of solid supports which are required for the immobilization, including porous monoliths, as well as two different immobilization schemes based on biotin–streptavidin interactions and covalent linkage via free amino groups respectively.作者: Irrepressible 時(shí)間: 2025-3-22 02:37 作者: 外表讀作 時(shí)間: 2025-3-22 07:20
Three-Dimensional Cell-Based Microarrays: Printing Pluripotent Stem Cells into 3D Microenvironmentsribe the methodology for production of alginate- and Matrigel-based 3-D cell microarrays for the study of mouse and human pluripotent stem cells on two different chip-based platforms. We further provide protocols for on-chip proliferation/viability analysis and the assessment of protein expression by immunofluorescence.作者: Brittle 時(shí)間: 2025-3-22 11:55
Fabrication of Biomolecule Microarrays for Cell Immobilization Using Automated Microcontact Printingtomation process. Here, we describe the production of biomolecule microarrays composed of extracellular matrix protein for the fabrication of cell microarrays by using an automated microcontact printing device. Large scale cell microarrays can be reproducibly obtained by this method.作者: 武器 時(shí)間: 2025-3-22 14:51
Hydrogel Printing Based on UV-Induced Projection for Cell-Based Microarray Fabricationad of a physical mask, which also offers a high degree of flexibility and repeatability. Furthermore, the ability of PEGDA film to hinder cell adhesion makes it possible to control area over which cells attach.作者: 武器 時(shí)間: 2025-3-22 19:43
https://doi.org/10.1057/9781137339652ribe the methodology for production of alginate- and Matrigel-based 3-D cell microarrays for the study of mouse and human pluripotent stem cells on two different chip-based platforms. We further provide protocols for on-chip proliferation/viability analysis and the assessment of protein expression by immunofluorescence.作者: 以煙熏消毒 時(shí)間: 2025-3-22 23:11 作者: GEM 時(shí)間: 2025-3-23 04:14
Julie Cruikshank,Tatiana Argounova-Lowad of a physical mask, which also offers a high degree of flexibility and repeatability. Furthermore, the ability of PEGDA film to hinder cell adhesion makes it possible to control area over which cells attach.作者: sleep-spindles 時(shí)間: 2025-3-23 08:36 作者: 難解 時(shí)間: 2025-3-23 10:31 作者: AVOID 時(shí)間: 2025-3-23 17:28
Julie Cruikshank,Tatiana Argounova-Low culture protocol and telomere length analysis to suit for high throughput screening of telomere length in large number of individual donors. This approach can be used to demonstrate the heterogeneity or changes of telomere length and its shortening as an exclusion criterion for selection of suitable donors for future stem cell therapies.作者: Anterior 時(shí)間: 2025-3-23 18:49
Next-Generation Live-Cell Microarray Technologiess is the ability to provide quantitative data in real time with high reliability and sensitivity, which are key parameters for any cell-based assay. An additional advantage of live-cell microarrays is their inherent capability for large-scale screening of single cells, multicell populations, as well as spheroids.作者: Audiometry 時(shí)間: 2025-3-23 23:21
3D Plasma Nanotextured? Polymeric Surfaces for Protein or Antibody Arrays, and Biomolecule and Cell n nanotextured surfaces for microarray fabrication and (b) cell adhesion on such surfaces. 3D plasma nanotextured? substrates are commercialized through Nanoplasmas private company, a spin-off of the National Centre for Scientific Research Demokritos.作者: 召集 時(shí)間: 2025-3-24 03:06 作者: 反復(fù)拉緊 時(shí)間: 2025-3-24 10:03
https://doi.org/10.1057/9781137326904e cell gene expression after mRNA transfection and time courses of single cell apoptosis. We discuss the more general use of the protocol for assessment of single cell dynamics with the help of fluorescent reporters.作者: 愛哭 時(shí)間: 2025-3-24 14:19
Beyond the Ideal Political Apologying channels, thus expanding this protocol to any CNC platform This technique represents a versatile, high-fidelity prototyping method that enables fast turnaround of prototype devices in a standard laboratory setting, while offering scalability for commercial manufacturing.作者: 使尷尬 時(shí)間: 2025-3-24 17:05 作者: 沉著 時(shí)間: 2025-3-24 20:35 作者: artifice 時(shí)間: 2025-3-25 00:00
Book 2018e-cell microarrays. The chapters in this book are categorized into six parts: introduction, biointerface engineering and live-cell microarrays, printing techniques for live-cell microarrays, live-cell microarray application, microfluidic live-cell arrays, and advanced materials suitable for live-cel作者: GUEER 時(shí)間: 2025-3-25 05:37
The Advantage of the Covered Call,g of flat platforms based on wettability contrast, compatible with the high-throughput generation and study of cell response in 3D biomaterials, including cell-laden hydrogels and porous 3D scaffolds. The application of the aforementioned platforms to produce 3D microtissues, which may find applicat作者: Perigee 時(shí)間: 2025-3-25 09:53
,A Nonconformist Women’s Literary Tradition,hyl ester (Calcein-AM) and treatment of MSCs with differentiation media to analyze the proliferation, viability, and differentiation potential of printed cells. This way, the general applicability of the established living cell-based microarray production was demonstrated.作者: 神秘 時(shí)間: 2025-3-25 14:48 作者: FOR 時(shí)間: 2025-3-25 18:21 作者: 方舟 時(shí)間: 2025-3-25 20:44 作者: aplomb 時(shí)間: 2025-3-26 01:17
Cell-Based Microarrays Using Superhydrophobic Platforms Patterned with Wettable Regionsg of flat platforms based on wettability contrast, compatible with the high-throughput generation and study of cell response in 3D biomaterials, including cell-laden hydrogels and porous 3D scaffolds. The application of the aforementioned platforms to produce 3D microtissues, which may find applicat作者: EXUDE 時(shí)間: 2025-3-26 05:58 作者: Malcontent 時(shí)間: 2025-3-26 09:01 作者: debris 時(shí)間: 2025-3-26 15:48 作者: 猜忌 時(shí)間: 2025-3-26 19:39 作者: 獨(dú)行者 時(shí)間: 2025-3-26 23:26
3D Plasma Nanotextured? Polymeric Surfaces for Protein or Antibody Arrays, and Biomolecule and Cell nd microarrays. Nanotextured surfaces with desirable chemical functionality (and wetting behavior) have shown excellent biomolecule immobilization and cell adhesion. Specifically, nanotextured hydrophilic areas show (a) strong binding of biomolecules and (b) strong adhesion of cells, while nanotextu作者: CRACY 時(shí)間: 2025-3-27 04:46 作者: Anal-Canal 時(shí)間: 2025-3-27 06:55
Artificial Microniche Array with Spatially Structured Biochemical Cueshese cavities range in size from single cell to multicellular aggregate. We provide detailed protocols to create such arrays with some variations using different materials and different coating proteins. The use of such cavities as bona fide artificial microniches to mimic cellular microenvironments作者: Fissure 時(shí)間: 2025-3-27 10:04 作者: Ganglion-Cyst 時(shí)間: 2025-3-27 14:28
Fabrication of Biomolecule Microarrays for Cell Immobilization Using Automated Microcontact Printingalternative way of deposition of biomolecules on solid supports but even if various biomolecules have been successfully microcontact printed, the production of biomolecule microarrays in routine by microcontact printing remains a challenging task and needs an effective, fast, robust, and low-cost au作者: 輕快走過 時(shí)間: 2025-3-27 18:36
Hydrogel Printing Based on UV-Induced Projection for Cell-Based Microarray Fabricationvery, and extracellular matrix construction. Here, we introduce a hydrogel printing method based on UV-induced projection via a digital micromirror device (DMD). Arbitrary microstructures could be fabricated within few seconds (<3) by modulating UV projection using DMD as digital dynamic masks inste作者: 淘氣 時(shí)間: 2025-3-27 22:33 作者: ANIM 時(shí)間: 2025-3-28 03:56
High-Throughput Screening of Senescence Markers in Hematopoietic Stem Cells Derived from Induced Pluisms that control cell fate decisions and differentiation toward distinct lineages. Here we focus on the ability of donors derived iPSCs to differentiate toward hematopoietic progenitor cells and on the analysis of their telomere length. The ability to screen telomere length in individual donors is 作者: ARCH 時(shí)間: 2025-3-28 09:48 作者: 易受騙 時(shí)間: 2025-3-28 10:46 作者: 過度 時(shí)間: 2025-3-28 18:06 作者: flammable 時(shí)間: 2025-3-28 21:07
Fabrication of Biomolecule Microarrays Using Rapid Photochemical Surface Patterning in Thiol–Ene-Bas protocol, we describe a photochemical surface patterning procedure based on thiol–ene/yne photochemistry which allows for the simple and rapid selective patterning of biomolecules on thiol–ene solid supports. We describe the preparation of solid supports which are required for the immobilization, i作者: Sad570 時(shí)間: 2025-3-28 23:57 作者: FLAX 時(shí)間: 2025-3-29 05:37
The Advantage of the Covered Call,This rapid, cost-saving and systematic way of acquiring biologically relevant information has found application in diverse scientific and industrial fields. In an initial stage of development, platforms targeting high-throughput cellular studies were restricted to standard two-dimensional (2D) setup作者: Alcove 時(shí)間: 2025-3-29 09:41 作者: 冷淡周邊 時(shí)間: 2025-3-29 13:39 作者: 掃興 時(shí)間: 2025-3-29 18:55
https://doi.org/10.1057/9781137336323hese cavities range in size from single cell to multicellular aggregate. We provide detailed protocols to create such arrays with some variations using different materials and different coating proteins. The use of such cavities as bona fide artificial microniches to mimic cellular microenvironments作者: Customary 時(shí)間: 2025-3-29 22:47 作者: 滴注 時(shí)間: 2025-3-30 03:07 作者: meretricious 時(shí)間: 2025-3-30 06:42
Julie Cruikshank,Tatiana Argounova-Lowvery, and extracellular matrix construction. Here, we introduce a hydrogel printing method based on UV-induced projection via a digital micromirror device (DMD). Arbitrary microstructures could be fabricated within few seconds (<3) by modulating UV projection using DMD as digital dynamic masks inste作者: synovial-joint 時(shí)間: 2025-3-30 09:38
https://doi.org/10.1057/9781137339652for cell patterning in the field of biomedical applications, either directly printing cells in cell media, or indirectly through printing a wax scaffold that guides cell orientation/attachment onto a substrate.作者: outrage 時(shí)間: 2025-3-30 16:17
Julie Cruikshank,Tatiana Argounova-Lowisms that control cell fate decisions and differentiation toward distinct lineages. Here we focus on the ability of donors derived iPSCs to differentiate toward hematopoietic progenitor cells and on the analysis of their telomere length. The ability to screen telomere length in individual donors is
