作者: Delirium 時(shí)間: 2025-3-21 20:29 作者: sulcus 時(shí)間: 2025-3-22 00:57
Ella PalmerProvides an up-to-date variety of protocols covering all aspects in thorough, clear detail -Presents readers with clear step-by-step protocols to allow them to set up difficult techniques and expand u作者: Lacunar-Stroke 時(shí)間: 2025-3-22 06:03 作者: 厭倦嗎你 時(shí)間: 2025-3-22 11:07
Organizations in the Face of Crisis. They reported a technique whereby expression vectors containing the open reading frame (ORF) of human genes were printed onto glass microscope slides to form a microarray. Transfection reagents were added pre- or post-spotting and cells grown over the surface of the array. They demonstrated that c作者: liposuction 時(shí)間: 2025-3-22 13:09
Organizations in the Face of Crisisification of gene functions is needed. Reverse transfection microarray technology, which turns a DNA microarray into a cell-based microarray, has emerged for simultaneously studying the function of many genes. Since the initial demonstration in 2001, many variations have surfaced, making the technol作者: liposuction 時(shí)間: 2025-3-22 17:52 作者: REIGN 時(shí)間: 2025-3-22 23:15
Organizations in the Face of Crisisassays have employed fluorescent proteins as reporters. These fluorescent reporter proteins can be monitored in living cells but have low sensitivity of detection compared to enzymatic reporters. Here we have described a novel transcriptional reporter assay using the alkaline phosphatase reporter en作者: 踉蹌 時(shí)間: 2025-3-23 03:51 作者: 危機(jī) 時(shí)間: 2025-3-23 09:25 作者: 分開如此和諧 時(shí)間: 2025-3-23 10:19 作者: 表臉 時(shí)間: 2025-3-23 15:12
Napoleon and Augustus: Empire Buildersn cell metabolism, especially under pathological conditions. Since primary cells reflect the physiological situation more closely than transformed cell lines, their employment in such screenings is highly desirable. A difficulty to overcome – besides the shortage in cell supply – is that primary cel作者: 規(guī)章 時(shí)間: 2025-3-23 19:01
G. Donald Chandler III,John W. Chandlerperative activities of several different viral genes which trigger the phenotypic functions of the infected cells. Systematic analyses of these complex interactions require high-throughput transfection technology. Here we have provided a laboratory manual for the reverse transfected cell microarray 作者: integral 時(shí)間: 2025-3-24 01:49 作者: 記憶 時(shí)間: 2025-3-24 06:24
G. Donald Chandler III,John W. Chandlerinto the blood stream. To that end, cell capture of several cell types at different locations on a microarray is an interesting alternative to classical techniques run ‘in solution’ as it allows simultaneous characterization of several cells on a single device. In this chapter, we have described a m作者: 生來(lái) 時(shí)間: 2025-3-24 07:55
Napoleon and Augustus: Empire Builderst cells due to the difficulty in immobilization of non-adherent cells. Herein, we have introduced our techniques that can rapidly and strongly immobilize non-adherent cells and can allow the transfection of non-adherent cells with plasmid cDNA and small interfering RNA (siRNA) at a defined position 作者: 敲詐 時(shí)間: 2025-3-24 13:20 作者: 搜集 時(shí)間: 2025-3-24 14:57 作者: 感激小女 時(shí)間: 2025-3-24 19:39 作者: Landlocked 時(shí)間: 2025-3-25 03:12 作者: instructive 時(shí)間: 2025-3-25 03:37 作者: LANCE 時(shí)間: 2025-3-25 10:41 作者: Limited 時(shí)間: 2025-3-25 12:37 作者: Incommensurate 時(shí)間: 2025-3-25 19:32 作者: conflate 時(shí)間: 2025-3-25 22:29
Cell-Based Microarrays: Overview,ene function and identification of novel therapeutic agents has now been clearly demonstrated. Current efforts are focused on improving and harnessing this technology for high-throughput screening applications.作者: Fretful 時(shí)間: 2025-3-26 02:54 作者: 絕緣 時(shí)間: 2025-3-26 07:09
Plasma Polymer and PEG-Based Coatings for DNA, Protein and Cell Microarrays,mation of a multifunctional layer by spin coating a PEG-based polymer that also displayed epoxy groups. The successful demonstration of DNA, protein and cell microarrays has been achieved on each of these coatings.作者: 友好 時(shí)間: 2025-3-26 10:57 作者: 我不死扛 時(shí)間: 2025-3-26 16:02
Organizations in the Face of Crisisermediate filaments and actin filaments. The application of these “organelle-co-localized cell arrays” facilitates the precise determination of the localizations of numerous recombinant proteins in a single experiment, making it currently the most efficient technique for high-throughput protein co-l作者: 土坯 時(shí)間: 2025-3-26 19:12 作者: 過(guò)渡時(shí)期 時(shí)間: 2025-3-26 21:09 作者: 換話題 時(shí)間: 2025-3-27 04:48
Book 2011is of over expression and knock down of proteins.In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of t作者: PRISE 時(shí)間: 2025-3-27 08:12
A Novel Fluorescent Transcriptional Reporter for Cell-Based Microarray Assays,zyme and a fluorescent substrate (ELF-97) to screen for gain-of-function mutations in the type-I cGMP-dependent protein kinase (PRKG1). We have identified a constitutively active mutant of this enzyme in which a conserved Glu at position 81 was mutated to Lys.作者: critic 時(shí)間: 2025-3-27 13:11 作者: 不如樂死去 時(shí)間: 2025-3-27 13:52 作者: Habituate 時(shí)間: 2025-3-27 20:19
Organizations in the Face of Crisisfor co-transfection of two plasmids into HEK293T cells. This cell-based microarray in a microplate may be used for screening hundreds of analytes against multiple protein targets in parallel, providing a powerful tool for functional genomics and drug discovery.作者: Inelasticity 時(shí)間: 2025-3-28 01:59 作者: Volatile-Oils 時(shí)間: 2025-3-28 05:08 作者: 小樣他閑聊 時(shí)間: 2025-3-28 08:26
https://doi.org/10.1057/9781137318763 U87 brain tumor cell lines by measuring EGFR, PTEN, pAKT, and pS6 with a MIC platform, and applied this measurement to clinical brain tumor specimens. In conjunction with statistical analysis, we were able to characterize extensive inter- and intra-tumoral molecular heterogeneity.作者: 慢慢啃 時(shí)間: 2025-3-28 11:19 作者: MAIZE 時(shí)間: 2025-3-28 18:20 作者: 染色體 時(shí)間: 2025-3-28 19:06
Cell-Based Microarrays of Infectious Adenovirus Encoding Short Hairpin RNA (shRNA),ic manipulation, efficient transfer of genetic information can be achieved without drastic effects on cell viability, and by using a microarray format even small amounts of cells suffice to perform a screen.作者: Resection 時(shí)間: 2025-3-29 02:25
Microfluidic Image Cytometry, U87 brain tumor cell lines by measuring EGFR, PTEN, pAKT, and pS6 with a MIC platform, and applied this measurement to clinical brain tumor specimens. In conjunction with statistical analysis, we were able to characterize extensive inter- and intra-tumoral molecular heterogeneity.作者: 才能 時(shí)間: 2025-3-29 05:19
1064-3745 ls to allow them to set up difficult techniques and expand uAs a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins.In Cell-Based Microarrays: Methods and Pr作者: 后來(lái) 時(shí)間: 2025-3-29 09:05
Napoleon and Augustus: Empire Buildersize non-adherent cells and can allow the transfection of non-adherent cells with plasmid cDNA and small interfering RNA (siRNA) at a defined position on substrates with poly(ethylene-glycol)-lipid-modified surface.作者: 疲憊的老馬 時(shí)間: 2025-3-29 14:00 作者: Accolade 時(shí)間: 2025-3-29 17:11
Opium and Empire in Southeast Asia our system under the condition of over-expression of GFP-tagged proteins. We measured their influence upon BFA-induced effects in a format, suitable for large-scale studies in living cell, namely cell arrays. The approach can be applied for large-scale RNA interference studies as well as for chemical screening.作者: 驚呼 時(shí)間: 2025-3-29 23:27
G. Donald Chandler III,John W. Chandlerrmine the effects of viral genes in transfected cells, protocols for the use of GFP as an indicator gene and for indirect immunofluorescence staining of cellular target proteins have been included. RTCM provides a useful methodological approach to investigate systematically combination effects of viral genes on cellular functions.作者: peak-flow 時(shí)間: 2025-3-30 00:58
G. Donald Chandler III,John W. Chandlered at each location. The result is an array whose features are micrometer-sized clusters of cells expressing defined genes. This approach provides for the high-throughput functional analysis of many different proteins in parallel and can be considerably more informative and cost-effective relative to more traditional protein expression techniques.作者: Rinne-Test 時(shí)間: 2025-3-30 06:41
‘It’s Good to Talk’ and Other Storiess between their chemical structure and their biological performance. The flexibility of polymer microarrays analysis not only greatly refines our knowledge of multitude of cell-biomaterial interactions but could also be used in biocompatibility assessments as novel biomarkers.作者: 提煉 時(shí)間: 2025-3-30 09:37 作者: Keratectomy 時(shí)間: 2025-3-30 15:26 作者: Legion 時(shí)間: 2025-3-30 19:19
Transfected Cell Microarrays for the Expression of Membrane-Displayed Single-Chain Antibodies,ed at each location. The result is an array whose features are micrometer-sized clusters of cells expressing defined genes. This approach provides for the high-throughput functional analysis of many different proteins in parallel and can be considerably more informative and cost-effective relative to more traditional protein expression techniques.作者: lambaste 時(shí)間: 2025-3-30 22:45 作者: cornucopia 時(shí)間: 2025-3-31 04:29
Organizations in the Face of Crisiszyme and a fluorescent substrate (ELF-97) to screen for gain-of-function mutations in the type-I cGMP-dependent protein kinase (PRKG1). We have identified a constitutively active mutant of this enzyme in which a conserved Glu at position 81 was mutated to Lys.作者: 哥哥噴涌而出 時(shí)間: 2025-3-31 05:25
Opium and Empire in Southeast Asiaing on single cell phenotypes, such as systematic localization or co-localization studies or genetic assays for genes affecting probed targets. We demonstrate the use of yeast spheroplast microarrays to probe microtubule and spindle defects across a collection of yeast strains harboring tetracycline-down-regulatable alleles of essential genes.作者: NIL 時(shí)間: 2025-3-31 10:37
G. Donald Chandler III,John W. Chandlerethod based on the production of antibody microarrays specific to two different cell types: B and T lymphocytes. We have also described the real-time monitoring of the cell capture on the microarray using surface plasmon resonance imaging (SPRi).
