作者: 發(fā)電機(jī) 時(shí)間: 2025-3-21 23:22
https://doi.org/10.1007/978-981-99-6434-5pter, the epi-fluorescence light path is introduced and the various components are discussed in detail. Recommendations are made for incident lamp light sources, excitation and emission filters, dichroic mirrors, objective lenses, and charge-coupled device (CCD) cameras in order to obtain the most s作者: Proclaim 時(shí)間: 2025-3-22 02:31
SpringerBriefs in Environmental Sciencen. Therefore, the specimen must be monitored for viability and health before, during, and after imaging sessions. Methods for monitoring cell viability and health will be discussed in this chapter. Another key to successful live-cell imaging is to minimize light exposure as much as possible. A summa作者: Cacophonous 時(shí)間: 2025-3-22 07:03
https://doi.org/10.1007/978-981-99-6434-5 in the same region via multifluorescence allows for the analysis of differential protein expression. The defining step of multifluorescence labeling is the selection of primary antibodies from different host species. In addition, species-appropriate secondary antibodies must also be conjugated to d作者: 陪審團(tuán) 時(shí)間: 2025-3-22 11:34 作者: Tinea-Capitis 時(shí)間: 2025-3-22 13:06 作者: Tinea-Capitis 時(shí)間: 2025-3-22 17:53 作者: famine 時(shí)間: 2025-3-23 01:00 作者: 反抗者 時(shí)間: 2025-3-23 03:49 作者: 天賦 時(shí)間: 2025-3-23 07:57
Obligation and Healthcare Reforms in China,on-fluorescent nanoparticle probes can be identified and tracked dynamically inside crowded living cells with either differential interference contrast (DIC) microscopy or planar illumination microscopy (PIM). The translational and rotational dynamics of anisotropic nanoparticles can be readily extr作者: otic-capsule 時(shí)間: 2025-3-23 12:09 作者: gnarled 時(shí)間: 2025-3-23 14:05
Obligation and Healthcare Reforms in China,er direct microscopic visualization. Histologically enriched cell populations can be procured by harvesting cells of interest directly or isolating specific cells by ablating unwanted cells. The basic components of laser microdissection technology are (a) visualization of cells via light microscopy,作者: Chemotherapy 時(shí)間: 2025-3-23 18:19 作者: Encumber 時(shí)間: 2025-3-24 00:16
Obligation and Healthcare Reforms in China,cle describes the approaches for chemical functionalization of the mica surface. One approach utilizes 3-aminopropyl-trietoxy silane (APTES), enabling one to obtain a smooth surface termed AP-mica. This surface binds nucleic acids and nucleoprotein complexes in a wide range of ionic strengths, in th作者: Yourself 時(shí)間: 2025-3-24 03:56 作者: 蝕刻 時(shí)間: 2025-3-24 08:14 作者: COLIC 時(shí)間: 2025-3-24 14:04
https://doi.org/10.1007/978-981-99-6437-6emand. As recently as just 15 years ago, it was believed that during cell secretion, membrane-bound secretory vesicles completely merge at the cell plasma membrane resulting in the diffusion of intravesicular contents to the cell exterior and the compensatory retrieval of the excess membrane by endo作者: 小卒 時(shí)間: 2025-3-24 18:10
https://doi.org/10.1007/978-981-99-6437-6he unbiased and efficient estimation of structural features without making any assumptions on the underlying nature of the biological sample. The methods are based on rigorous sampling of location and orientation, the application of appropriate test systems, and the controlling of the precision of t作者: enhance 時(shí)間: 2025-3-24 21:51
Thomas Bartz-Beielstein,Eva Bartzhis chapter incorporating an unbiased approach to quantitative sural nerve evaluation. Using conventional epoxy embedded nerves processed for electron microscopy, confocal microscopy, and interactive digital assessment, this method produces a rigorous, accurate reproducible record for use in clinica作者: 1FAWN 時(shí)間: 2025-3-25 02:59
Cell Imaging Techniques978-1-62703-056-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 誓言 時(shí)間: 2025-3-25 07:12
SpringerBriefs in Environmental Scienceesigned to image subcellular structures, such as endosomes and secretory vesicles in the salivary glands (SGs) of live rats. To this aim, we used fluorescently labeled molecules and/or fluorescently tagged proteins that were transiently transfected in the live animal.作者: 懶惰人民 時(shí)間: 2025-3-25 10:04
Thomas Bartz-Beielstein,Eva Bartzhis chapter incorporating an unbiased approach to quantitative sural nerve evaluation. Using conventional epoxy embedded nerves processed for electron microscopy, confocal microscopy, and interactive digital assessment, this method produces a rigorous, accurate reproducible record for use in clinical diagnosis.作者: ABIDE 時(shí)間: 2025-3-25 11:42 作者: faultfinder 時(shí)間: 2025-3-25 18:14 作者: correspondent 時(shí)間: 2025-3-25 20:23 作者: 種屬關(guān)系 時(shí)間: 2025-3-26 01:24 作者: cringe 時(shí)間: 2025-3-26 08:15 作者: 文件夾 時(shí)間: 2025-3-26 10:20 作者: AVID 時(shí)間: 2025-3-26 13:55 作者: FLIRT 時(shí)間: 2025-3-26 18:05 作者: 陳腐思想 時(shí)間: 2025-3-26 21:41 作者: famine 時(shí)間: 2025-3-27 02:31
Epi-Fluorescence Microscopy, being the best choice for sensitive, high resolution multicolor imaging. Images should be collected using Nyquist sampling and images should be corrected for background intensity contributions and nonuniform illumination across the field of view. Photostable fluorescent probes and proteins that abs作者: 巡回 時(shí)間: 2025-3-27 08:14
Live-Cell Migration and Adhesion Turnover Assays,escribed on how to prepare samples for single cell migration assays, how to measure cell migration rates (e.g., bright-field, semi-automated, and automated), and how to measure focal adhesion turnover rates. Details of how to correct images for background intensity and field-illumination uniformity 作者: ULCER 時(shí)間: 2025-3-27 12:27
,Laser Scanning Cytometry: Principles and Applications—An Update,nalysis of progeny of individual cells in clonogenicity assay; (g) cell immunophenotyping; (h) imaging, visual examination, or sequential analysis using different probes of the same cells upon their relocation; (i) in situ enzyme kinetics, drug uptake, and other time-resolved processes; (j) analysis作者: neuron 時(shí)間: 2025-3-27 14:20
Laser Capture Microdissection for Protein and NanoString RNA Analysis, heterogeneous sample. Laser energy in the capture method is infrared (810 nm), while in the cutting mode the laser is ultraviolet (355 nm). Infrared lasers melt a thermolabile polymer that adheres to the cells of interest, whereas ultraviolet lasers ablate cells for either removal of unwanted cells作者: Etching 時(shí)間: 2025-3-27 18:40 作者: Concomitant 時(shí)間: 2025-3-28 00:24 作者: exophthalmos 時(shí)間: 2025-3-28 05:03 作者: Dna262 時(shí)間: 2025-3-28 06:24 作者: dyspareunia 時(shí)間: 2025-3-28 11:11
SpringerBriefs in Environmental Scienceescribed on how to prepare samples for single cell migration assays, how to measure cell migration rates (e.g., bright-field, semi-automated, and automated), and how to measure focal adhesion turnover rates. Details of how to correct images for background intensity and field-illumination uniformity 作者: 許可 時(shí)間: 2025-3-28 18:25 作者: 誘拐 時(shí)間: 2025-3-28 20:23
Obligation and Healthcare Reforms in China, heterogeneous sample. Laser energy in the capture method is infrared (810 nm), while in the cutting mode the laser is ultraviolet (355 nm). Infrared lasers melt a thermolabile polymer that adheres to the cells of interest, whereas ultraviolet lasers ablate cells for either removal of unwanted cells作者: 數(shù)量 時(shí)間: 2025-3-29 01:15 作者: Crayon 時(shí)間: 2025-3-29 05:16 作者: CHASM 時(shí)間: 2025-3-29 11:04
Digital Images Are Data: And Should Be Treated as Such,the papers contained at least one figure that did not comply with the journal’s instructions to authors. The scientific press continues to report a small, but steady stream of cases of fraudulent image manipulation. Inappropriate image manipulation taints the scientific record, damages trust within 作者: LAPSE 時(shí)間: 2025-3-29 12:08 作者: Lament 時(shí)間: 2025-3-29 19:01 作者: 鋪?zhàn)?nbsp; 時(shí)間: 2025-3-29 19:46
Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic Protei in the same region via multifluorescence allows for the analysis of differential protein expression. The defining step of multifluorescence labeling is the selection of primary antibodies from different host species. In addition, species-appropriate secondary antibodies must also be conjugated to d作者: RENAL 時(shí)間: 2025-3-30 01:48 作者: 支架 時(shí)間: 2025-3-30 04:06
A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown, envelope breakdown, one of the major morphological changes of mitosis. Here, we describe such a strategy in which the plasma membrane of cells expressing fluorescently tagged nucleoporin POM121 and Histone H2B is permeabilized with digitonin. These cells are then incubated with mitotic . egg extrac作者: 造反,叛亂 時(shí)間: 2025-3-30 09:55 作者: Spongy-Bone 時(shí)間: 2025-3-30 13:09
Image-Based High-Throughput Screening for Inhibitors of Angiogenesis, co-cultured vascular cells form capillary-like networks that model facets of angiogenesis, making it an attractive alternative for anti-angiogenic drug discovery. We have adapted this angiogenesis assay system to a high-throughput format to enable automated image-based high-throughput screening of 作者: 露天歷史劇 時(shí)間: 2025-3-30 19:23
Intravital Microscopy to Image Membrane Trafficking in Live Rats,esigned to image subcellular structures, such as endosomes and secretory vesicles in the salivary glands (SGs) of live rats. To this aim, we used fluorescently labeled molecules and/or fluorescently tagged proteins that were transiently transfected in the live animal.作者: Fibrillation 時(shí)間: 2025-3-30 23:09
Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential Interfon-fluorescent nanoparticle probes can be identified and tracked dynamically inside crowded living cells with either differential interference contrast (DIC) microscopy or planar illumination microscopy (PIM). The translational and rotational dynamics of anisotropic nanoparticles can be readily extr作者: florid 時(shí)間: 2025-3-31 02:49
,Laser Scanning Cytometry: Principles and Applications—An Update,cytometry (FCM). This review describes attributes of LSC and covers its numerous applications derived from plentitude of the parameters that can be measured. Among many LSC applications the following are emphasized: (a) assessment of chromatin condensation to identify mitotic, apoptotic cells, or se作者: Prologue 時(shí)間: 2025-3-31 05:58 作者: Capture 時(shí)間: 2025-3-31 13:07
Viewing Dynamic Interactions of Proteins and a Model Lipid Membrane with Atomic Force Microscopy,ssfully applied to more than one type of lipid study and atomic force microscope (AFM) instrument setup. The basic procedural steps have been used with an Asylum Research MFP-3D BIO and the Bruker (.) BioScope. The AFM imaging protocol has been supplemented by procedures (.) of ellipsometry, standar作者: 安心地散步 時(shí)間: 2025-3-31 15:29
Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy,cle describes the approaches for chemical functionalization of the mica surface. One approach utilizes 3-aminopropyl-trietoxy silane (APTES), enabling one to obtain a smooth surface termed AP-mica. This surface binds nucleic acids and nucleoprotein complexes in a wide range of ionic strengths, in th作者: 拾落穗 時(shí)間: 2025-3-31 18:09
Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation, procedures for using AFM indentation to measure the elastic moduli of living cells. We include step-by-step instructions for cantilever calibration and data acquisition using a combined AFM/optical microscope system, as well as a detailed protocol for data analysis. Our protocol is written specific作者: 蜿蜒而流 時(shí)間: 2025-4-1 00:47
Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells,racy (in order of several nm). During the past 5 years, simultaneous topography and recognition imaging (TREC) has become a powerful AFM-based technique for quick and easy high-resolution receptor mapping. In this chapter, we provide a flavor of TREC application on vascular endothelial cells by desc作者: Compass 時(shí)間: 2025-4-1 04:43
