作者: Small-Intestine 時(shí)間: 2025-3-21 21:29
Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriationparticles, with minimal perturbation of cellular functions. The physical characteristics of cell size and sedimentation velocity are operative in the technique of centrifugal elutriation also known as counterstreaming centrifugation. The elutriator is an advanced device for increasing the sedimentat作者: gait-cycle 時(shí)間: 2025-3-22 02:35
Image Cytofluorometry for the Quantification of Ploidy and Endoplasmic Reticulum Stress in Cancer Ceed by asymmetric divisions and chromosome loss. Increases in ploidy are consistently accompanied by the activation of an endoplasmic reticulum (ER) stress response, resulting in the translocation of calreticulin to the outer surface of the plasma membrane where it stimulates anticancer immune respon作者: 瑣碎 時(shí)間: 2025-3-22 08:20 作者: 過(guò)度 時(shí)間: 2025-3-22 11:59 作者: 多樣 時(shí)間: 2025-3-22 13:32
Synchronization of Mammalian Cell Cultures by Serum Deprivationhe cycle. Procedures to arrest cultured cells in specific phases of the cell cycle may be termed in vitro synchronization. The procedure described here was developed for the study of primary astrocytes and a glioma cell line, but is broadly applicable to other mammalian cells. Its application allows作者: 多樣 時(shí)間: 2025-3-22 19:22
DNA Damage Response Resulting from Replication Stress Induced by Synchronization of Cells by Inhibithidicolin or excess of thymidine the cells that become arrested at the entrance to S-phase upon release from the block initiate progression through S then G. and M. However, exposure to these inhibitors at concentrations commonly used to synchronize cells leads to activation of ATR and ATM protein k作者: Cholesterol 時(shí)間: 2025-3-23 01:06
Chromosome Formation During Fertilization in Eggs of the Teleost , been accumulated by various experiments using inhibitors to affect formation and behavior of chromosomes in the cycle of cell proliferation. Based on experimental results on meiosis and early stages of development of the teleost ., we discuss the roles of the activities of histone H1 kinase, microt作者: 流利圓滑 時(shí)間: 2025-3-23 01:43
Flow Cytometry Analysis of Cell Cycle and Specific Cell Synchronization with Butyraten MDBK cells. We explore the possibility of using butyrate-blocked cells to obtain synchronized cells and we characterize the properties of butyrate-induced cell cycle arrest. The site of growth inhibition and cell cycle arrest was analyzed using 5-bromo-2′-deoxyuridine (BrdU) incorporation and flow作者: cyanosis 時(shí)間: 2025-3-23 06:28
Chemically Induced Cell Cycle Arrest in Perfusion Cell Culturehat would meet all requirements does not exist, we have established a long-term scanning-perfusion platform that: (a) replaces old medium with fresh one, (b) bypasses physical contact with the cell culture during continuous cell growth, (c) provides uninterrupted photomicrography of single cells, an作者: 作嘔 時(shí)間: 2025-3-23 13:24
Analysis of Nuclear Uracil DNA–Glycosylase (nUDG) Turnover During the Cell Cycleing genomic integrity by reducing the mutagenic events caused by G:C to A:T transition mutations. The recent finding that a family of RNA editing enzymes (AID/APOBECs) can deaminate cytosine in DNA has raised the interest in these base-excision repair enzymes. The methodology presented here focuses 作者: 媽媽不開(kāi)心 時(shí)間: 2025-3-23 15:03
Synchronization of HeLa Cells various methods, HeLa cells have been used extensively for studying the cell cycle. Here, we describe several protocols for synchronizing HeLa cells from different phases of the cell cycle, including G. phase using the HMG-CoA reductase inhibitor lovastatin, S phase with a double thymidine block pr作者: 沒(méi)血色 時(shí)間: 2025-3-23 18:52
Synchronization of , Cells by Spore Germination and Outgrowthts taking place during the transition from spore to vegetative cells. The transition stages involve: phase change, swelling, emergence, initial division, and elongation. By using this method we have obtained two distinctive synchronized cell cycles, while the synchrony faded away in the third cycle.作者: modish 時(shí)間: 2025-3-23 23:17 作者: 連詞 時(shí)間: 2025-3-24 04:21
Synchronization of Pathogenic Protozoansg sickness). These organisms are distantly related to humans but with retained eukaryotic type of cellular processes, making them good model systems for studies of the evolution of basic processes like the cell cycle. . causes 250 million cases of diarrhea yearly and is one of the earliest diverging作者: 初次登臺(tái) 時(shí)間: 2025-3-24 09:15
1064-3745 ation advice from the experts.Includes supplementary materiaThis second edition volume provides detailed protocols on the theoretical background of cell cycle synchronization procedures and instructions on how to implement these techniques. The chapters in?.Cell Cycle Synchronization: Methods and Pr作者: Irrepressible 時(shí)間: 2025-3-24 13:59 作者: Sinus-Node 時(shí)間: 2025-3-24 15:17
https://doi.org/10.1007/978-981-10-3599-9or studies of the evolution of basic processes like the cell cycle. . causes 250 million cases of diarrhea yearly and is one of the earliest diverging protozoans. It is possible to synchronize its cell cycle using compounds that inhibit different steps of the cell cycle and the detailed protocol is described here.作者: ingestion 時(shí)間: 2025-3-24 20:21 作者: 確定無(wú)疑 時(shí)間: 2025-3-25 01:40
Synchronization of Pathogenic Protozoansor studies of the evolution of basic processes like the cell cycle. . causes 250 million cases of diarrhea yearly and is one of the earliest diverging protozoans. It is possible to synchronize its cell cycle using compounds that inhibit different steps of the cell cycle and the detailed protocol is described here.作者: 書(shū)法 時(shí)間: 2025-3-25 06:05
Chemically Induced Cell Cycle Arrest in Perfusion Cell Cultured (d) secures near physiological conditions and sterility up to several weeks. The system was validated by synchronizing cells using serum starvation and butyrate-induced cell cycle arrest of HaCaT cells.作者: 催眠藥 時(shí)間: 2025-3-25 10:07
Sumathi Ganasen,Mageswary Karpudewand (d) secures near physiological conditions and sterility up to several weeks. The system was validated by synchronizing cells using serum starvation and butyrate-induced cell cycle arrest of HaCaT cells.作者: aesthetician 時(shí)間: 2025-3-25 12:11 作者: 媒介 時(shí)間: 2025-3-25 18:32
Sustainable Supply Chain Management,hes helps in generalizing results. An overview of the most commonly used methods to generate synchronized yeast cultures is presented along with working .: a section that includes practical comments, experimental considerations and observations, and hints regarding the pros and cons innate to each approach.作者: OUTRE 時(shí)間: 2025-3-25 20:59
Large-Scale Mitotic Cell Synchronization laboratory for isolating mitotic mammalian cells in a large-scale manner. In particular, we discuss the technical aspects of adherent or suspension cell isolation, the methods necessary to enrich cells at different mitotic stages and the optimized culture conditions.作者: 性別 時(shí)間: 2025-3-26 01:32 作者: ALE 時(shí)間: 2025-3-26 04:19 作者: STIT 時(shí)間: 2025-3-26 08:42 作者: 燕麥 時(shí)間: 2025-3-26 12:40
Sumathi Ganasen,Mageswary Karpudewanevels decrease to barely detectable levels during the S phase of the cell cycle. Immunoblot analysis of immunoprecipitated or affinity-isolated nUDG reveals ubiquitin-conjugated nUDG when proteolysis is inhibited by agents that block proteasomal-dependent protein degradation.作者: 兇猛 時(shí)間: 2025-3-26 19:05
https://doi.org/10.1007/978-981-10-3437-4cells from these blocks enables researchers to follow gene expression and other events through the cell cycle. We also describe several protocols, including flow cytometry, BrdU labeling, immunoblotting, and time-lapse microscopy, for validating the synchrony of the cells and monitoring the progression of the cell cycle.作者: MUT 時(shí)間: 2025-3-26 21:44
https://doi.org/10.1007/978-981-10-3599-9es stay synchronized, while synchrony rapidly decays during a few divisions. The limitations of this method are that it can be applied only for sporulating bacteria and synchrony lasts for only a limited period of time exceeding not more than two cycles.作者: strdulate 時(shí)間: 2025-3-27 05:09
Image Cytofluorometry for the Quantification of Ploidy and Endoplasmic Reticulum Stress in Cancer CeR stress and ploidy, we developed an image cytofluorometric method that allows to measure DNA content, ER stress-associated phosphorylation of eIF2α, and calreticulin exposure at the cell surface. Here, we specify this methodology, which is useful for investigating the correlation between ploidy and ER stress at the single cell level.作者: Dungeon 時(shí)間: 2025-3-27 07:37 作者: Alienated 時(shí)間: 2025-3-27 09:57
Synchronization of HeLa Cellscells from these blocks enables researchers to follow gene expression and other events through the cell cycle. We also describe several protocols, including flow cytometry, BrdU labeling, immunoblotting, and time-lapse microscopy, for validating the synchrony of the cells and monitoring the progression of the cell cycle.作者: 聲明 時(shí)間: 2025-3-27 15:59
Synchronization of , Cells by Spore Germination and Outgrowthes stay synchronized, while synchrony rapidly decays during a few divisions. The limitations of this method are that it can be applied only for sporulating bacteria and synchrony lasts for only a limited period of time exceeding not more than two cycles.作者: 越自我 時(shí)間: 2025-3-27 19:43 作者: 支架 時(shí)間: 2025-3-27 22:16
Variability of Glaciers and Snow Cover,utriation, early log S phase cell populations are most suitable where most of the cells are in G1 and S phase (>80 %). Apoptotic cells can be found in the early elutriation fractions belonging to the sub-Go window. Protocols for the synchronization of nuclei of murine pre-B cells and high-resolution作者: Cytology 時(shí)間: 2025-3-28 04:29
Zhi Wang,Wenwu Zhu,Shiqiang Yanghe assessment of cell cycle distribution, quantification of mitotic cell fraction, and analysis of single cell fate profile of living cells. We applied these methods to validate the synchronization-desynchronization protocol and to qualitatively and quantitatively determine the impact of SAC inactiv作者: Urgency 時(shí)間: 2025-3-28 09:48
Orbital Data Applications for Space Objects intrinsic limitations associated with these methods. In the present protocol, we describe a simple, reliable, and reversible procedure to synchronize astrocyte and glioma cultures from newborn rat brain by serum deprivation. The procedure is similar, and generally applicable, to other mammalian cel作者: 免費(fèi) 時(shí)間: 2025-3-28 11:36
Calculation of Collision Probability,synchronization methods. It also presents the protocol describing an assessment of phosphorylation of .139 on H2AX and activation of ATM in cells treated with aphidicolin, as a demonstrative of one of several DNA replication inhibitors that are being used for cell synchronization. Phosphorylation of作者: Airtight 時(shí)間: 2025-3-28 17:15
https://doi.org/10.1007/978-981-10-3437-4ls to begin the progression into the S phase. One cycle of cell division for MDBK cells is about 20 h. By combining BrdU incorporation and DNA content analysis, not only can the overlapping of different cell populations be eliminated, but the frequency and nature of individual cells that have synthe作者: 孤僻 時(shí)間: 2025-3-28 21:16 作者: 個(gè)人長(zhǎng)篇演說(shuō) 時(shí)間: 2025-3-29 00:48 作者: 拔出 時(shí)間: 2025-3-29 03:06 作者: 尖叫 時(shí)間: 2025-3-29 09:41 作者: 粗野 時(shí)間: 2025-3-29 13:11 作者: mutineer 時(shí)間: 2025-3-29 16:32 作者: 狂熱文化 時(shí)間: 2025-3-29 21:03 作者: Tincture 時(shí)間: 2025-3-30 02:03
1064-3745 hronization: Methods and Protocols, Second Edition.?is a valuable resource for PhD students and postdoctoral fellows, and researchers interested in general science, pharmacy, medicine and public health, compute978-1-4939-8248-6978-1-4939-6603-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: refraction 時(shí)間: 2025-3-30 04:48 作者: 實(shí)施生效 時(shí)間: 2025-3-30 09:19 作者: agglomerate 時(shí)間: 2025-3-30 14:19 作者: Urologist 時(shí)間: 2025-3-30 18:52
https://doi.org/10.1007/978-1-4939-6603-5Unicellular organisms; Transfected cells; Stem cells; Physical fractionation; centrifugal elutriation of作者: 種屬關(guān)系 時(shí)間: 2025-3-30 21:21
978-1-4939-8248-6Springer Science+Business Media New York 2017作者: 過(guò)濾 時(shí)間: 2025-3-31 02:33
Erhard Friedberg,Mary E. Hilderbrandonization distinct subpopulations of cells are obtained representing different stages of the cell cycle. These subpopulations are then used to study regulatory mechanisms of the cycle at the level of macromolecular biosynthesis (DNA synthesis, gene expression, protein synthesis), protein phosphoryla作者: 流動(dòng)才波動(dòng) 時(shí)間: 2025-3-31 08:34
Variability of Glaciers and Snow Cover,particles, with minimal perturbation of cellular functions. The physical characteristics of cell size and sedimentation velocity are operative in the technique of centrifugal elutriation also known as counterstreaming centrifugation. The elutriator is an advanced device for increasing the sedimentat
