作者: aspect 時(shí)間: 2025-3-21 22:16 作者: Anhydrous 時(shí)間: 2025-3-22 01:03
https://doi.org/10.1007/978-94-6209-622-6llators comprising of protein kinases and their regulators. Different cell cycle oscillators are inextricably linked to ensure orderly activation of oscillators. A recurring theme in their regulation is the abundance of autoamplifying loops that ensure switch-like and unidirectional cell cycle trans作者: Organization 時(shí)間: 2025-3-22 08:34
Origins: A Sustainable Concept in Educationn-dependent kinases (Cdks) and other cell cycle kinases. Here, I present the rationale and the experimental framework for an alternative approach to measure kinase activity that is based on estimating substrate phosphorylation rates in vivo. The approach presented was first developed for experiments作者: 山頂可休息 時(shí)間: 2025-3-22 10:13
Fred Dervin,Hanna Ragnarsdóttirthe APC/C targets its two essential substrates, securin and cyclin B, for proteasomal destruction. Cyclin B is the activator of cyclin-dependent kinase 1 (Cdk1), the major mitotic kinase, and both cyclin B and securin are safeguards of sister chromatid cohesion. Conversely, the degradation of securi作者: Pudendal-Nerve 時(shí)間: 2025-3-22 14:14
https://doi.org/10.1007/978-94-6209-854-1ission. A wide variety of genetically-encoded and synthetic biosensors have been developed to monitor protein kinase activity. We have focused on the design, engineering and characterization of fluorescent peptide biosensors of cyclin-dependent kinases (CDKs) that constitute attractive cancer biomar作者: Pudendal-Nerve 時(shí)間: 2025-3-22 19:28 作者: 他去就結(jié)束 時(shí)間: 2025-3-23 01:11 作者: 恭維 時(shí)間: 2025-3-23 02:08
On the Facilitation of the Academypression of mRNA expression. However, full-length 3′UTR vectors are rarely employed due to labor-intensive cloning work. Instead, 3′UTR fragments containing putative miRNA binding sites are commonly utilized to mechanistically validate miRNAs. Assaying truncated 3′UTRs may falsely validate miRNAs du作者: 健談 時(shí)間: 2025-3-23 07:03 作者: 碎石頭 時(shí)間: 2025-3-23 12:40 作者: 走調(diào) 時(shí)間: 2025-3-23 15:35 作者: COUCH 時(shí)間: 2025-3-23 20:15 作者: adhesive 時(shí)間: 2025-3-24 00:37 作者: escalate 時(shí)間: 2025-3-24 04:17 作者: In-Situ 時(shí)間: 2025-3-24 06:29 作者: 擴(kuò)音器 時(shí)間: 2025-3-24 13:10 作者: 得罪 時(shí)間: 2025-3-24 15:07 作者: 細(xì)胞學(xué) 時(shí)間: 2025-3-24 19:14
Cameron Mccarthy,Brenda Nyandiko Sanyaaging of subresolutional cellular components by conventional microscopes. Centrioles are small microtubule-based cylindrical structures that build centrosomes and cilia, two organelles essential for vertebrates. Due to a centriole’s small size, electron microscopy has traditionally been used to stud作者: CHART 時(shí)間: 2025-3-24 23:48
Sherry Marx,Monica Housen,Christine Taput kinase (cyclin–Cdk) complexes act as master regulators of the cell division cycle by phosphorylating numerous substrates. Their activity and expression profiles are regulated in time. The budding yeast . was one of the pioneering model organisms to study the cell cycle. Its genetic amenability con作者: Indebted 時(shí)間: 2025-3-25 05:24
https://doi.org/10.1007/978-1-0716-1538-6Cellular interactions; Oscillatory networks; Developmental biology; Interlinking oscillators; Cell cycle作者: evasive 時(shí)間: 2025-3-25 10:54 作者: GLADE 時(shí)間: 2025-3-25 14:56 作者: Cumulus 時(shí)間: 2025-3-25 17:35
1064-3745 expertsThis book brings together a unique collection of protocols that cover novel and specialized techniques as well as updated and improved adaptations of more standard procedures involving the cell cycle and its regulation by oscillatory networks, exploring recent progress in the field from both作者: 新星 時(shí)間: 2025-3-25 20:26 作者: 混合 時(shí)間: 2025-3-26 01:36 作者: 原始 時(shí)間: 2025-3-26 06:14 作者: superfluous 時(shí)間: 2025-3-26 09:45
Visualization of Radiation-Induced Cell Cycle Kinetics with a Fluorescent Ubiquitination-Based Cell G2 arrest in tumor cells with deficient p53 function. Here we describe protocols for establishing Fucci-expressing cell lines and analyzing radiation-induced G2 arrest kinetics in three different models: monolayer cell cultures, spheroids, and xenografted solid tumors in mice.作者: Forage飼料 時(shí)間: 2025-3-26 14:32
,Construction of a Full-Length 3′UTR Reporter System for Identification of Cell-Cycle Regulating Mice to altered positioning of binding sites in respect to 3′UTR length and RNA secondary structure. Here we present a detailed protocol for the construction of full-length 3′UTR luciferase reporter constructs that was used to unveil miRNAs regulating multiple cell-cycle factors.作者: cyanosis 時(shí)間: 2025-3-26 17:01
Assaying Cell Cycle Progression via Flow Cytometry in CRISPR/Cas9-Treated Cells,d assay that enables the determination of cell cycle progression in the presence of CRISPR/Cas9 treatment, in addition to the transfection and expression efficiencies of Cas9 vectors. This assay can also easily determine the effect of various interventions on obtaining a larger pool of Cas9-treated cells.作者: BIPED 時(shí)間: 2025-3-26 21:08 作者: intricacy 時(shí)間: 2025-3-27 04:00
The New Iconography of the Global Cityd assay that enables the determination of cell cycle progression in the presence of CRISPR/Cas9 treatment, in addition to the transfection and expression efficiencies of Cas9 vectors. This assay can also easily determine the effect of various interventions on obtaining a larger pool of Cas9-treated cells.作者: 木質(zhì) 時(shí)間: 2025-3-27 05:51 作者: pester 時(shí)間: 2025-3-27 11:33
https://doi.org/10.1007/978-94-6209-854-1s a straightforward means of comparing CDK activity in different cell lines and evaluating the specific impact of treatments intended to target kinase activity in a physiologically relevant environment.作者: 不溶解 時(shí)間: 2025-3-27 17:20
https://doi.org/10.1007/978-94-6265-044-2TP analog 3-BrB-PP1, generates higher levels of synchrony with timing and morphology much more reminiscent of a normal division. We also describe a version of the H1 kinase assay of Cdk1-Cyclin B activity that is widely used to monitor mitotic progression which does not require radiolabeled ATP.作者: Negotiate 時(shí)間: 2025-3-27 21:23
Governance of Open Data Initiatives,le analysis. Useful modifications to the original protocol (Pereira et al., Oncotarget, 8:40514–40,532, 2017) have been introduced to increase flexibility in data collection and facilitate data analysis.作者: Stable-Angina 時(shí)間: 2025-3-28 00:15
Substrate Phosphorylation Rates as an In Vivo Measurement of Kinase Activity,tion is possible. Briefly this involves transient and reversible kinase inhibition to dephosphorylate kinase substrates in vivo, followed by quantitative measurements of phosphorylation after inhibition is removed.作者: 培養(yǎng) 時(shí)間: 2025-3-28 05:16 作者: insecticide 時(shí)間: 2025-3-28 09:45
Highly Synchronous Mitotic Progression in , Upon Relief of Transient Cdc2-asM17 Inhibition,TP analog 3-BrB-PP1, generates higher levels of synchrony with timing and morphology much more reminiscent of a normal division. We also describe a version of the H1 kinase assay of Cdk1-Cyclin B activity that is widely used to monitor mitotic progression which does not require radiolabeled ATP.作者: strdulate 時(shí)間: 2025-3-28 10:54 作者: 多山 時(shí)間: 2025-3-28 15:27
Book 2021Latest editionmore standard procedures involving the cell cycle and its regulation by oscillatory networks, exploring recent progress in the field from both holistic and reductionist perspectives. The edition provides a space for researchers to highlight and explore the latest developments in molecular biology an作者: 半導(dǎo)體 時(shí)間: 2025-3-28 21:36
On the Facilitation of the Academywhole-mount immunostaining combined with 5-ethynyl-2′-deoxyuridine (EdU) staining, which enables the visualization of spatial patterns in histone modifications in the early and late S-phase nuclei of . roots.作者: 魔鬼在游行 時(shí)間: 2025-3-29 02:06 作者: 撤退 時(shí)間: 2025-3-29 03:09
Sherry Marx,Monica Housen,Christine Tapu phosphorylation events that govern the cell cycle. In this chapter, we introduce robust and straightforward methods to analyze cell cycle progression in .. These techniques can be utilized to describe cell cycle events and to address the effects of perturbations on accurate and timely cell cycle progression.作者: STERN 時(shí)間: 2025-3-29 09:06
Whole-Mount Immunostaining for the Identification of Histone Modifications in the S-Phase Nuclei ofwhole-mount immunostaining combined with 5-ethynyl-2′-deoxyuridine (EdU) staining, which enables the visualization of spatial patterns in histone modifications in the early and late S-phase nuclei of . roots.作者: arbovirus 時(shí)間: 2025-3-29 11:53 作者: 有組織 時(shí)間: 2025-3-29 18:05
Analysis of Cell Cycle Progression in the Budding Yeast , phosphorylation events that govern the cell cycle. In this chapter, we introduce robust and straightforward methods to analyze cell cycle progression in .. These techniques can be utilized to describe cell cycle events and to address the effects of perturbations on accurate and timely cell cycle progression.作者: 百科全書(shū) 時(shí)間: 2025-3-29 21:05
Cell Cycle Control: A System of Interlinking Oscillators,llators comprising of protein kinases and their regulators. Different cell cycle oscillators are inextricably linked to ensure orderly activation of oscillators. A recurring theme in their regulation is the abundance of autoamplifying loops that ensure switch-like and unidirectional cell cycle trans作者: nitric-oxide 時(shí)間: 2025-3-30 03:14
Substrate Phosphorylation Rates as an In Vivo Measurement of Kinase Activity,n-dependent kinases (Cdks) and other cell cycle kinases. Here, I present the rationale and the experimental framework for an alternative approach to measure kinase activity that is based on estimating substrate phosphorylation rates in vivo. The approach presented was first developed for experiments作者: 使痛苦 時(shí)間: 2025-3-30 04:17
Real-Time Monitoring of APC/C-Mediated Substrate Degradation Using , Egg Extracts,the APC/C targets its two essential substrates, securin and cyclin B, for proteasomal destruction. Cyclin B is the activator of cyclin-dependent kinase 1 (Cdk1), the major mitotic kinase, and both cyclin B and securin are safeguards of sister chromatid cohesion. Conversely, the degradation of securi作者: Hemiplegia 時(shí)間: 2025-3-30 09:32 作者: arcane 時(shí)間: 2025-3-30 12:25
Phosphatase and Kinase Substrate Specificity Profiling with Pooled Synthetic Peptides and Mass Spec. Determining the inherent substrate specificity of kinases and phosphatases is essential for understanding their cellular roles. Synthetic peptides have long served as substrate proxies for defining intrinsic kinase and phosphatase specificities. Here, we describe a high throughput protocol to simu作者: SLUMP 時(shí)間: 2025-3-30 16:34
Whole-Mount Immunostaining for the Identification of Histone Modifications in the S-Phase Nuclei ofevious studies have demonstrated that dramatic changes in local chromatin structure are required for the initiation and progression of DNA replication, and that histone modifications play an essential role in the determination of chromatin structure in S phase. Since euchromatic and heterochromatic 作者: 王得到 時(shí)間: 2025-3-30 20:56 作者: venous-leak 時(shí)間: 2025-3-31 04:40 作者: Seminar 時(shí)間: 2025-3-31 08:37
Optimizing Cell Synchronization Using Nocodazole or Double Thymidine Block,eve the cell culture synchronization but not all type of cells respond equally to a given concentration of these drugs. Here we describe a simple optimization method to select concentrations and timings for nocodazole or thymidine treatments using fluorescence staining. In addition, we provide detai作者: CHASM 時(shí)間: 2025-3-31 12:53 作者: Muffle 時(shí)間: 2025-3-31 17:03
Elucidating Human Mitosis Using an Anaphase-Like Cell-Free System,ory and structural proteins. These series of events ultimately secure genome stability and time-invariant cellular properties during cell proliferation. Two of the core enzymes regulating mitotic milestones in all eukaryotes are cyclin dependent kinase 1 (CDK1) with its coactivator cyclin B, and the作者: 聽(tīng)覺(jué) 時(shí)間: 2025-3-31 18:08
,EDU (5-Ethynyl-2′-Deoxyuridine)-Coupled Fluorescence-Intensity Analysis: Determining Absolute Paramycle stages (G1, S, and G2) are detailed herein. This methodology utilizes flow cytometry to take full advantage of the excellent stoichiometric properties of click chemistry. This allows detection, via azide-fluorochrome coupling, of the modified deoxynucleoside 5-ethynyl-2′-deoxyuridine (EDU) inco作者: 仔細(xì)閱讀 時(shí)間: 2025-3-31 22:49 作者: Adenocarcinoma 時(shí)間: 2025-4-1 04:05 作者: Regurgitation 時(shí)間: 2025-4-1 07:25
