標(biāo)題: Titlebook: Cell Cycle Control and Dysregulation Protocols; Antonio Giordano,Gaetano Romano Book 2004 Humana Press 2004 [打印本頁(yè)] 作者: FORAY 時(shí)間: 2025-3-21 16:52
書(shū)目名稱Cell Cycle Control and Dysregulation Protocols影響因子(影響力)
書(shū)目名稱Cell Cycle Control and Dysregulation Protocols影響因子(影響力)學(xué)科排名
書(shū)目名稱Cell Cycle Control and Dysregulation Protocols網(wǎng)絡(luò)公開(kāi)度
書(shū)目名稱Cell Cycle Control and Dysregulation Protocols網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱Cell Cycle Control and Dysregulation Protocols被引頻次
書(shū)目名稱Cell Cycle Control and Dysregulation Protocols被引頻次學(xué)科排名
書(shū)目名稱Cell Cycle Control and Dysregulation Protocols年度引用
書(shū)目名稱Cell Cycle Control and Dysregulation Protocols年度引用學(xué)科排名
書(shū)目名稱Cell Cycle Control and Dysregulation Protocols讀者反饋
書(shū)目名稱Cell Cycle Control and Dysregulation Protocols讀者反饋學(xué)科排名
作者: abreast 時(shí)間: 2025-3-21 23:51 作者: 扔掉掐死你 時(shí)間: 2025-3-22 01:57
Immunofluorescence Analysisine). Nonradioactive ribonucleic acid (RNA) precursors (e.g., 5-bromouridine-5′-triphosphate [BrUTP]) are used and can be detected by using fluorescently labeled antibodies. Procedures for BrUTP of labeling transcription sites require manipulations that are best applied to adherent cells but can be 作者: 兇猛 時(shí)間: 2025-3-22 06:44 作者: 窩轉(zhuǎn)脊椎動(dòng)物 時(shí)間: 2025-3-22 11:41
Analysis of In Vivo Gene Expression Using Epitope-Tagged Proteinsand green fluorescent protein (GFP). Both molecules pose limitations with in vivo detection. When using GFP, autofluorescence may be encountered in tissues and tissue sections. Similarly, when using β-gal as a marker, the penetration of X-gal substrate is difficult in tissues with extensive extra ce作者: artifice 時(shí)間: 2025-3-22 16:12 作者: artifice 時(shí)間: 2025-3-22 20:59
Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expressiontors with cognate regulatory elements). This assay is based on reduced electrophoretic mobilities of protein/DNA complexes through a nondenaturing polyacrylamide gel compared with unbound DNA fragments or double-stranded oligonucleotides. If multiprotein complexes bind, the migration rate slows furt作者: 虛構(gòu)的東西 時(shí)間: 2025-3-22 23:49
Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expressionth loose or more open conformation are sensitive to DNase I cleavage. By comparing the patterns of hypersensitive sites obtained by digestion of silent genes with those obtained when the genes are actively transcribed, regions that participate in gene regulation (either induction or suppression) can作者: 創(chuàng)作 時(shí)間: 2025-3-23 01:41
Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expressiony in vivo protein-deoxyribonucleic acid (DNA) interactions at regions of genes important for transcriptional regulation. Successful analysis of in vivo occupancy of gene regulatory elements by LM-PCR largely depends on the strategy and quality of reagents. The experimental outline for LM-PCR reactio作者: 發(fā)誓放棄 時(shí)間: 2025-3-23 05:45
Assays for Cyclin-Dependent Kinase Inhibitorse if associated with its cyclin partner and if the complex is phosphorylated at specific activating residues (threonine 160/161; .. and .). The progression through the cell cycle is mediated by the sequential activation of CDKs during different phases of the cycle. The G1/S phase transition of the c作者: MERIT 時(shí)間: 2025-3-23 12:50
Protein Degradation Via the Proteosomel cycle (.). The importance of protein degradation in the control of cell proliferation is elegantly illustrated by the regulated synthesis and destruction of the cyclin family of proteins during the life cycle of a cell. The cyclins are a group of proteins that influence the function of a series of作者: 連詞 時(shí)間: 2025-3-23 16:42 作者: 重畫(huà)只能放棄 時(shí)間: 2025-3-23 21:10 作者: Explicate 時(shí)間: 2025-3-24 01:39 作者: 大暴雨 時(shí)間: 2025-3-24 03:59
Induction of Deoxyribonucleic Acid Damage by Alkylating Agentsr electrophilic nature, alkylating agents have an affinity for the nucleophilic centers of DNA and are monofunctional (have one reactive group) or bifunctional (have two reactive groups). Alkylating agents, such as methylmethanesulphonate and .-ethyl-.-nitrosourea, introduce methyl, ethyl, and compl作者: 敲詐 時(shí)間: 2025-3-24 08:57 作者: hypotension 時(shí)間: 2025-3-24 12:26 作者: 黃油沒(méi)有 時(shí)間: 2025-3-24 15:06
1064-3745 e, kinases, and kinase inhibitors. It addresses the issue of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Becaus作者: GLADE 時(shí)間: 2025-3-24 21:47
The Essence of Acts of Empathy,tly labeled antibodies. Procedures for BrUTP of labeling transcription sites require manipulations that are best applied to adherent cells but can be applied, with difficulty, to cell cultures in suspension. The protocol described below is for adherent cells grown on cover slips.作者: abreast 時(shí)間: 2025-3-25 00:35
https://doi.org/10.1007/978-94-017-6067-6o occupancy of gene regulatory elements by LM-PCR largely depends on the strategy and quality of reagents. The experimental outline for LM-PCR reaction is shown in .. For a through understanding of this procedure and other alternative options, . Ausubel et al. (.).作者: 云狀 時(shí)間: 2025-3-25 04:02 作者: 無(wú)動(dòng)于衷 時(shí)間: 2025-3-25 10:07 作者: 五行打油詩(shī) 時(shí)間: 2025-3-25 12:34
Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expressiono occupancy of gene regulatory elements by LM-PCR largely depends on the strategy and quality of reagents. The experimental outline for LM-PCR reaction is shown in .. For a through understanding of this procedure and other alternative options, . Ausubel et al. (.).作者: 意外 時(shí)間: 2025-3-25 19:29
Detection of Apoptotic Deoxyribonucleic Acid Break by , Nick Translationtivation may contribute to a number of diseases, such as AIDS, neurodegenerative disorders, and ischemic injury (.). However, impaired apoptosis may be a significant factor in the etiology of diseases like cancer, autoimmune disorders, and viral infections (.).作者: eardrum 時(shí)間: 2025-3-25 22:17
Book 2004sses the issue of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Because there are already many manuals and protoc作者: FORGO 時(shí)間: 2025-3-26 01:24 作者: Engaging 時(shí)間: 2025-3-26 05:12 作者: 骯臟 時(shí)間: 2025-3-26 12:07 作者: 多產(chǎn)子 時(shí)間: 2025-3-26 16:33 作者: 信條 時(shí)間: 2025-3-26 18:48
Immunofluorescence Analysisnd staining with specific antibodies before microscopic examination. To reveal the subcellular and subnuclear macromolecular complexes that comprise and govern activation of the regulatory machinery for gene expression, cells can be subjected to selective extractions before immunodetection as described below.作者: LANCE 時(shí)間: 2025-3-26 22:03 作者: Consensus 時(shí)間: 2025-3-27 04:39
The Transformed Phenotypely derived from oncogenic viruses. Thus the definition of transformation in mammalian cells differs form the definition of transformation in bacteria where the term refers to the introduction of any new gene into the bacterial genome.作者: 精確 時(shí)間: 2025-3-27 07:40 作者: endoscopy 時(shí)間: 2025-3-27 11:10
Order-disorder in hexagonal lattices proteins (green fluorescent protein [GFP], cyan fluorescent protein, yellow fluorescent protein, and red fluorescent protein). Caution must be exercised when tagging large proteins (e.g., GFP or β-galactosidase) at the amino or carboxy termini to avoid interference with folding that can influence a作者: 卡死偷電 時(shí)間: 2025-3-27 15:47 作者: 魅力 時(shí)間: 2025-3-27 21:27 作者: subacute 時(shí)間: 2025-3-27 22:42
https://doi.org/10.1007/978-94-017-6065-2re OC is not transcribed). However, two DNase I hypersensitive sites are detected in the OC gene in bone tissues and in mature osteoblasts where the gene is actively transcribed. The first DNase I region encompasses the basal promoter regions that contain regulatory sequences for the RUNX, C/EBP, ac作者: Vo2-Max 時(shí)間: 2025-3-28 05:57 作者: generic 時(shí)間: 2025-3-28 07:15
https://doi.org/10.1007/978-94-017-6071-3arply circumscribed, uniformly-dense masses that abut on the nuclear envelope and condensation of the cytoplasm. Continuation of condensation is accompanied by convolution of the nuclear and cellular outlines, and nucleus often break up at this stage to produce discrete fragments. The surface protub作者: 思想流動(dòng) 時(shí)間: 2025-3-28 12:10
The Biology of Cyclins and Cyclin-Dependent Protein Kinasesycle. Moreover, it is now widely recognized that cyclins and CDKs do much more than simply control cell cycle progression. The relatively simple mechanisms discovered in yeast cells, which have one G1 cyclin, one G2 cyclin, and a single CDK, are replaced in mammalian cells by a richly redundant mole作者: 范圍廣 時(shí)間: 2025-3-28 17:16
Immunofluorescence Analysis Using Epitope-Tagged Proteins proteins (green fluorescent protein [GFP], cyan fluorescent protein, yellow fluorescent protein, and red fluorescent protein). Caution must be exercised when tagging large proteins (e.g., GFP or β-galactosidase) at the amino or carboxy termini to avoid interference with folding that can influence a作者: 極深 時(shí)間: 2025-3-28 19:49
Chromatin Immunoprecipitationosomes. Formaldehyde crosslinking rapidly fixes protein-protein and protein-DNA complexes in vivo providing the basis for an approach to analyze native structure of gene promoters. The protocol below is a simple yet sensitive method to determine whether a known protein is associated with a DNA seque作者: 增減字母法 時(shí)間: 2025-3-29 02:18
Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expressionences would decrease the band intensity of the previously shifted complexes, whereas mutated or unrelated binding sequences will not change the intensity of the previously shifted complexes (an example is shown in .).作者: 調(diào)整校對(duì) 時(shí)間: 2025-3-29 04:34 作者: Daily-Value 時(shí)間: 2025-3-29 07:37 作者: 影響 時(shí)間: 2025-3-29 12:25
A Morphologic Approach to Detect Apoptosis Based on Electron Microscopyarply circumscribed, uniformly-dense masses that abut on the nuclear envelope and condensation of the cytoplasm. Continuation of condensation is accompanied by convolution of the nuclear and cellular outlines, and nucleus often break up at this stage to produce discrete fragments. The surface protub作者: AFFIX 時(shí)間: 2025-3-29 15:43
Book 2004fident that Cell Cycle Control and Dysregulation Protocols will facilitate and optimize the work of practical scientists involved in researching the cell cycle. We greatly acknowledge the extraordinary contribution of the authors in writing this book.作者: PURG 時(shí)間: 2025-3-29 21:27 作者: 殺蟲(chóng)劑 時(shí)間: 2025-3-30 00:59 作者: pellagra 時(shí)間: 2025-3-30 07:56 作者: 不透明性 時(shí)間: 2025-3-30 11:58 作者: Formidable 時(shí)間: 2025-3-30 16:04 作者: liposuction 時(shí)間: 2025-3-30 17:44 作者: entitle 時(shí)間: 2025-3-30 20:42
Antonio Giordano,Gaetano RomanoIncludes supplementary material: 作者: MUT 時(shí)間: 2025-3-31 04:11 作者: 制定 時(shí)間: 2025-3-31 08:35 作者: 叫喊 時(shí)間: 2025-3-31 11:53
The Essence of Acts of Empathy,f nuclear architecture and cytoarchitecture. High-resolution . immunofluorescence approaches permit assessment of functional interrelationships between nuclear structure and gene expression that are linked to the intranuclear compartmentalization of nucleic acids and regulatory proteins (an example 作者: 排出 時(shí)間: 2025-3-31 14:06
The Essence of Acts of Empathy,ine). Nonradioactive ribonucleic acid (RNA) precursors (e.g., 5-bromouridine-5′-triphosphate [BrUTP]) are used and can be detected by using fluorescently labeled antibodies. Procedures for BrUTP of labeling transcription sites require manipulations that are best applied to adherent cells but can be 作者: SPURN 時(shí)間: 2025-3-31 18:39 作者: 使痛苦 時(shí)間: 2025-4-1 01:03
https://doi.org/10.1007/978-94-017-6063-8and green fluorescent protein (GFP). Both molecules pose limitations with in vivo detection. When using GFP, autofluorescence may be encountered in tissues and tissue sections. Similarly, when using β-gal as a marker, the penetration of X-gal substrate is difficult in tissues with extensive extra ce作者: SNEER 時(shí)間: 2025-4-1 03:16
https://doi.org/10.1007/978-94-017-6063-8her order chromatin controls accessibility to DNA binding proteins that influence regulatory events that include transcription, replication, recombination, and DNA repair. Chromatin is remodeled in a physiologically responsive manner by covalently modifying histones to neutralize a positive charge o
