標題: Titlebook: Cell Cycle Checkpoints; Methods and Protocol James J. Manfredi Book 2021 The Editor(s) (if applicable) and The Author(s) 2021 Flow cytometr [打印本頁] 作者: ergonomics 時間: 2025-3-21 18:28
書目名稱Cell Cycle Checkpoints影響因子(影響力)
書目名稱Cell Cycle Checkpoints影響因子(影響力)學科排名
書目名稱Cell Cycle Checkpoints網(wǎng)絡公開度
書目名稱Cell Cycle Checkpoints網(wǎng)絡公開度學科排名
書目名稱Cell Cycle Checkpoints被引頻次
書目名稱Cell Cycle Checkpoints被引頻次學科排名
書目名稱Cell Cycle Checkpoints年度引用
書目名稱Cell Cycle Checkpoints年度引用學科排名
書目名稱Cell Cycle Checkpoints讀者反饋
書目名稱Cell Cycle Checkpoints讀者反饋學科排名
作者: 樂章 時間: 2025-3-21 23:25
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/222781.jpg作者: 法律的瑕疵 時間: 2025-3-22 03:26
https://doi.org/10.1007/978-1-0716-1217-0Flow cytometric methods; Phosphorylation; Protein Degradation; Transcription; Cdk4/6 complexes作者: contradict 時間: 2025-3-22 07:07
978-1-0716-1219-4The Editor(s) (if applicable) and The Author(s) 2021作者: PANT 時間: 2025-3-22 08:47 作者: forestry 時間: 2025-3-22 13:23
“Aristotelian” Themes in Husserl’s ,rtility. p53 undergoes extensive regulatory post-translational modification through events such as phosphorylation, acetylation, methylation, and ubiquitylation. Here, we describe western blotting-based methodology for the detection and relative quantification of individual phosphorylation events in作者: forestry 時間: 2025-3-22 19:13 作者: 注意到 時間: 2025-3-22 22:38
https://doi.org/10.1007/978-94-017-0103-7 thereby on carcinogenesis. More recently, the replication machinery has been discovered as an interesting target for cancer therapeutic strategies. Given its high biological and clinical relevance, technologies for the analysis of DNA replication have attracted major attention. The so-called DNA fi作者: 受辱 時間: 2025-3-23 01:35
https://doi.org/10.1007/978-94-017-0103-7te of translation of mRNAs is critical for understanding the factors and pathways involved in gene expression. In this chapter, we present a method to compare the rate of translation of individual mRNA species based on the fraction of mRNA bound by a specific ribonucleoprotein involved in the genera作者: 商店街 時間: 2025-3-23 05:51 作者: 漂白 時間: 2025-3-23 12:42 作者: COUCH 時間: 2025-3-23 15:29 作者: Cholagogue 時間: 2025-3-23 18:28
Open Multithreaded Transactionshemical signals. These signals regulate the onset of each cell cycle phase and prevent undesired phase transitions. Functional complementation is a robust strategy to identify such signals, by which mutant phenotypes are rescued through complementation with candidate factors. Here we describe a meth作者: 粗俗人 時間: 2025-3-23 23:37
Meiosis was another unexpected propertyion of specific gene products. Short interfering RNA (siRNA) pathway is a RNAi pathway, where exogenous double stranded RNA is introduced to the cell and cleaved by an endoribonuclease, Dicer, to form siRNA, which interacts with a protein complex to scan mRNAs to bind to its complementary sequence. 作者: overbearing 時間: 2025-3-24 04:09
https://doi.org/10.1007/978-94-017-0167-9ous analysis of cellular DNA content using a fluorescent DNA dye (propidium iodide) and of a nuclear proliferation marker (Ki-67). The second is based on the differential staining of DNA and RNA using Hoechst 33342 and Pyronin Y: this method is particularly useful to distinguish quiescent cells in G作者: 透明 時間: 2025-3-24 08:53
The description of cell divisionnome” by either arresting cells in the cell cycle in order to allow time for repair of DNA damage or regulating a process of programmed cell death known as apoptosis. This process will eliminate cells that have suffered severe damage from intrinsic or extrinsic factors such as X-ray irradiation or c作者: PAN 時間: 2025-3-24 14:28 作者: Monotonous 時間: 2025-3-24 15:11
Modeling Aerosol Drug Delivery, instance after DNA damage or perturbations of the cell cycle. Mitotic catastrophe can avoid the generation of neoplastic cells from premalignant precursors, yet may also occur in cancer cells as a result of radiotherapy or chemotherapy. Of note, vinca alkaloids and taxanes, which are both known for作者: 污穢 時間: 2025-3-24 19:38
Kees B. Geerse,Jan C. M. Marijnissen have developed adaptive signaling pathways to cope with stress. The phosphorylation on serine 51 of the eukaryotic translation initiation factor (eIF2α) is a highly conserved event in stress responses, including the one that is activated upon treatment with mitotic catastrophe inducing agents, such作者: Agnosia 時間: 2025-3-25 02:45
https://doi.org/10.1007/978-94-017-0504-2ure checkpoint” and “spindle assembly checkpoint” (SAC) leading to aberrant mitosis. Depending on the signal, MC can drive the cell to death or to senescence. The suppression of MC favors aneuploidy. Several cancer therapies, included microtubular poisons and radiations, trigger MC. The clonogenic a作者: 蕁麻 時間: 2025-3-25 04:17 作者: 使激動 時間: 2025-3-25 08:22
Book 2021-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Cell Cycle Checkpoints: Methods and Protocols. is a valuable resource for researcher interested in learning more about this developing field..作者: deriver 時間: 2025-3-25 14:55 作者: 委托 時間: 2025-3-25 15:49
Open Multithreaded Transactionso DNA damage. Cell-free extracts derived from the eggs of . remain a robust and versatile system to decipher the biochemical steps underlying this essential characteristic of all cells, critical for genome stability.作者: Concrete 時間: 2025-3-25 23:01
Modeling Aerosol Drug Delivery,y characterized by the exposure of the endoplasmic reticulum protein calreticulin on the cell surface. Here we describe an immunofluorescence-based cytofluorometric technique to detect calreticulin exposure on tumor cells exposed to drugs that induce mitotic catastrophe.作者: defuse 時間: 2025-3-26 02:33
DNA Affinity Purification: A Pulldown Assay for Identifying and Analyzing Proteins Binding to Nuclerves as a link for indirect transcriptional repression of target genes by the tumor suppressor p53. In general, the methods described can be applied for the identification and analysis of proteins binding to DNA.作者: 暖昧關系 時間: 2025-3-26 05:58 作者: 摘要記錄 時間: 2025-3-26 10:50 作者: 不發(fā)音 時間: 2025-3-26 16:04
Detection of Post-translationally Modified p53 by Western Blotting, p53. While we focus on well-established N-terminal modifications for the purpose of illustration, this approach can be used to investigate other post-translational modifications of the protein, drawing upon a broad range of commercially available modification-specific antibodies.作者: calumniate 時間: 2025-3-26 17:46 作者: Vsd168 時間: 2025-3-26 21:25 作者: 一夫一妻制 時間: 2025-3-27 05:08
A Novel Strategy to Track Lysine-48 Ubiquitination by Fluorescence Resonance Energy Transfer,ymes and substrates, as well as for the execution of the reaction with high efficiency. Tracking K48 polyubiquitination using this sensitive and highly reproducible format provides an opportunity for high-throughput screening that leads to identification of small molecule modulators capable of changing ubiquitination for improving human health.作者: 嫻熟 時間: 2025-3-27 05:54
Knockdown of Target Genes by siRNA In Vitro,The binding of the siRNA to its complementary mRNA, the mRNA is cleaved and degraded by the cell, significantly reducing the levels of the target protein product. The discovery of this mechanism made it a powerful tool to use as a technique for therapeutics, agricultural biology, and cellular and molecular biology.作者: filicide 時間: 2025-3-27 11:10 作者: HAUNT 時間: 2025-3-27 14:43 作者: 精密 時間: 2025-3-27 19:36 作者: AGATE 時間: 2025-3-27 23:04 作者: SPASM 時間: 2025-3-28 05:52 作者: 微生物 時間: 2025-3-28 09:56 作者: Androgen 時間: 2025-3-28 11:26
Analysis of Replication Dynamics Using the Single-Molecule DNA Fiber Spreading Assay,ucleotide analogs which later can be fluorescently stained and analyzed. This chapter outlines the use of the DNA fiber spreading technique for the analysis of replication dynamics and replication structures.作者: nocturnal 時間: 2025-3-28 18:14 作者: 溝通 時間: 2025-3-28 21:54 作者: Aerate 時間: 2025-3-29 02:09
Clonogenic Assays to Detect Cell Fate in Mitotic Catastrophe,r drugs. Nevertheless, this method cannot analyze MC events. Here, we report an improved technique based on the use of human colon cancer HCT116 stable expressing histone H2B-GFP and DsRed-centrin proteins, allowing to determine the capacity of cells to proliferate, and to determine changes in the nucleus and centrosomes.作者: MONY 時間: 2025-3-29 03:46
Book 2021over topics such as post-translationally modified p53 by western blotting; CHK1 cellular localization by immunofluorescence microscopy; DNA affinity purification; knockdown of target genes by siRNA in vitro; and calreticulin exposure in mitotic catastrophe. Written in the highly successful .Methods 作者: Pathogen 時間: 2025-3-29 10:15 作者: 溝通 時間: 2025-3-29 14:36
Husserl’s Concept of Categorial Intuition identifying genes that are direct transcriptional targets of p53. We provide detailed procedures for measuring global RNA polymerase activity, defining p53 binding sites across the genome, and quantifying changes in steady-state mRNA in response to p53 activation.作者: CRAMP 時間: 2025-3-29 19:04 作者: 截斷 時間: 2025-3-29 20:29
Open Multithreaded Transactionsymes and substrates, as well as for the execution of the reaction with high efficiency. Tracking K48 polyubiquitination using this sensitive and highly reproducible format provides an opportunity for high-throughput screening that leads to identification of small molecule modulators capable of changing ubiquitination for improving human health.作者: 獨輪車 時間: 2025-3-30 03:41
Meiosis was another unexpected propertyThe binding of the siRNA to its complementary mRNA, the mRNA is cleaved and degraded by the cell, significantly reducing the levels of the target protein product. The discovery of this mechanism made it a powerful tool to use as a technique for therapeutics, agricultural biology, and cellular and molecular biology.作者: 災禍 時間: 2025-3-30 04:26 作者: reptile 時間: 2025-3-30 11:56
Kees B. Geerse,Jan C. M. Marijnissen as microtubular poisons or actin blockers. The protocol described herein details a method to quantify the phosphorylation of eIF2α by high-throughput immunofluorescence microscopy. This method is useful to capture the ‘integrated stress response’, which is characterized?by eIF2α phosphorylation in the context of mitotic catastrophe.作者: compel 時間: 2025-3-30 12:40
1064-3745 ation advice from the expertsThis volume explores the latest advancements in the field of cell cycle checkpoints and their implications for human diseases. Chapters in this book cover topics such as post-translationally modified p53 by western blotting; CHK1 cellular localization by immunofluorescen作者: 護身符 時間: 2025-3-30 16:37
The Puzzling Case of Alterity in Husserl’s ,ventional method for studying the cellular localization of CHK1, a cell cycle checkpoint kinase that undergoes shuttling from the cytoplasm to the nucleus in response to genotoxic stress. In this study, we included an acid extraction step to better reveal the nuclear localization of CHK1.
