標(biāo)題: Titlebook: Cell Culture Engineering IV; Improvements of Huma Barry C. Buckland,John G. Aunins,Weichang Zhou Book 1995 Springer Science+Business Media [打印本頁(yè)] 作者: 憑票入場(chǎng) 時(shí)間: 2025-3-21 19:56
書目名稱Cell Culture Engineering IV影響因子(影響力)
作者: 負(fù)擔(dān) 時(shí)間: 2025-3-21 21:38
https://doi.org/10.1007/978-94-011-0257-5biology; biotechnology; cell culture; development; molecular biology; pharmaceutical作者: Platelet 時(shí)間: 2025-3-22 04:00
978-94-010-4114-0Springer Science+Business Media Dordrecht 1995作者: Enliven 時(shí)間: 2025-3-22 08:27
Cell Culture Engineering IV978-94-011-0257-5Series ISSN 1386-2928 作者: AXIS 時(shí)間: 2025-3-22 11:59 作者: periodontitis 時(shí)間: 2025-3-22 16:53
Observatories in Earth Orbit and Beyondl growth halted and cell viability dropped. This was found to be associated with depletion of insulin from the medium early during the affected passage. Insulin depletion was found to be primarily the result of insulin degrading activity released by the cells during the preceding passage.作者: periodontitis 時(shí)間: 2025-3-22 20:42
The International Ultraviolet Explorer (IUE)ed a significant influence on metabolism and cell growth. The low-temperature-cultivations showed a lower growth rate and a lower glucose consumption rate and, therefore, less lactate production. On the other hand, the maximum cell density and productivity seemed not to be affected by the temperature reduction.作者: wangle 時(shí)間: 2025-3-22 23:05 作者: 向外供接觸 時(shí)間: 2025-3-23 05:22 作者: pulmonary 時(shí)間: 2025-3-23 08:24 作者: Dealing 時(shí)間: 2025-3-23 11:19 作者: 取之不竭 時(shí)間: 2025-3-23 16:19
Dan Tzivoni,Jesaia Benhorin,Shlomo Sternver, FAs have other effects on cells that may be less familiar. Polyunsaturated FAs of dietary origin (.-6 and .-3) cannot be synthesized by mammals, and are termed ‘essential’ because they are required for the optimal biologic function of specialized cells and tissues. However, they do not appear t作者: negligence 時(shí)間: 2025-3-23 19:52 作者: STALE 時(shí)間: 2025-3-23 23:24 作者: recession 時(shí)間: 2025-3-24 02:56
Dan Tzivoni,Jesaia Benhorin,Shlomo Stern(FCS) concentration. The specific growth rate varied with the initial FCS concentration in a manner which could be well fitted by the Monod model. The .. and μ. values were found to be 0.771% (v/v) serum and 0.031 h. respectively. The average specific IFN-γ production rates during the exponential ph作者: extinct 時(shí)間: 2025-3-24 07:22 作者: 侵略主義 時(shí)間: 2025-3-24 11:53 作者: 猛擊 時(shí)間: 2025-3-24 14:56 作者: Flatter 時(shí)間: 2025-3-24 22:37
Observatories in Earth Orbit and Beyondl growth halted and cell viability dropped. This was found to be associated with depletion of insulin from the medium early during the affected passage. Insulin depletion was found to be primarily the result of insulin degrading activity released by the cells during the preceding passage.作者: analogous 時(shí)間: 2025-3-25 00:42 作者: circuit 時(shí)間: 2025-3-25 03:24
Observatories in Earth Orbit and Beyondspontaneously in late exponential phase of batch cultures. Until lately, no specific triggering factors had been identified. Recently, we observed that glutamine, cystine or glucose deprivation induced apoptosis in both hybridoma and myeloma cell lines whereas accumulation of toxic metabolites induc作者: Phenothiazines 時(shí)間: 2025-3-25 09:38 作者: 新手 時(shí)間: 2025-3-25 14:31
The Topology of Galaxy Clustering,t S2 cell line is simple and quick, and in batch fermentations the cells have a doubling time of 20 hours until reaching a plateau density of 20 million cells/ml. Protein expression is driven by the . Metaliothionein promoter which is tightly regulated. When expressed in S2 cells, the extracellular 作者: aggressor 時(shí)間: 2025-3-25 19:32
The Topology of Galaxy Clustering,new constructions of adenovirus vectors, yielded as much as 10 to 20% recombinant protein with respect to the total cellular protein content. Until recently, high specific productivity of recombinant protein was limited to low cell density infected cultures of no more than 5 × 10. cells/mL. In this 作者: indignant 時(shí)間: 2025-3-25 21:09 作者: Lipoprotein 時(shí)間: 2025-3-26 04:06 作者: 材料等 時(shí)間: 2025-3-26 05:27 作者: Alopecia-Areata 時(shí)間: 2025-3-26 09:37 作者: hemorrhage 時(shí)間: 2025-3-26 15:43 作者: chlorosis 時(shí)間: 2025-3-26 19:22
1386-2928 ding of the molecular biology, the development ofnovel vaccines, approaches to bioreactor design and operation,monitoring techniques in process control and quality related topics.The work was carefully put together as one result of the Cell CultureEngineering IV Meeting held in San Diego, U.S.A. in 作者: Gingivitis 時(shí)間: 2025-3-26 22:02 作者: 密碼 時(shí)間: 2025-3-27 04:19 作者: 防水 時(shí)間: 2025-3-27 06:00 作者: alliance 時(shí)間: 2025-3-27 11:48 作者: dermatomyositis 時(shí)間: 2025-3-27 16:05
Recombinant protein expression in a , cell line: comparison with the baculovirus system,eceptor integrates correctly in the S2 membrane, binds [.H]spiperone with high affinity and exhibits pharmacological characteristics identical to that of the receptor expressed in Sf9 and mammalian cells. The general implications for large scale production of recombinant proteins are discussed.作者: BURSA 時(shí)間: 2025-3-27 17:59 作者: 過(guò)份艷麗 時(shí)間: 2025-3-27 22:55
Book 1995ver, thebook may not be perceived as a proceedings volume - thecriteria of the book series apply. .For cell biologists, biochemists, molecular biologists, immunologistsand other disciplines related to cell culture engineering, working inthe academic environment, as well as in (biotechnology orpharmaceutical) industry.作者: CHAR 時(shí)間: 2025-3-28 04:14
Dan Tzivoni,Jesaia Benhorin,Shlomo Sternof the culture except the lag phase and it was clearly demonstrated that IFN-γ production was growth associated. Specific glucose and glutamine utilisation rates were inversely related to specific growth rates.作者: 混沌 時(shí)間: 2025-3-28 07:57 作者: creditor 時(shí)間: 2025-3-28 11:07
David W. Gjertson,Paul I. Terasakie of glutamate. Choline independent cells were isolated from a choline limited chemostat. In an alternative approach to modifying cell behaviour, we have used recombinant DNA techniques to introduce the glutamine synthetase (GS) gene to a hybridoma. This resulted in glutamine independence and increased productivity.作者: 終點(diǎn) 時(shí)間: 2025-3-28 16:59 作者: Intellectual 時(shí)間: 2025-3-28 22:16 作者: dandruff 時(shí)間: 2025-3-29 01:46 作者: homocysteine 時(shí)間: 2025-3-29 04:21
Selecting and designing cell lines for improved physiological characteristics, natural variants with useful properties. Cholesterol independent variants of the mouse NSO myeloma cell line were isolated by cloning in a selective medium. A glutamine independent variant of a hyridoma was isolated by continuous (chemostat) culture under glutamine limited conditions in the presenc作者: 口訣 時(shí)間: 2025-3-29 10:36
Applications of improved stoichiometric model in medium design and fed-batch cultivation of animal h experiments were performed in T-flasks using this model in supplemental medium design (Xie and Wang, 1994b). In this work, the major pathways of glucose and glutamine metabolism were incorporated into the stoichiometric model. Fed-batch culture was conducted in a 2-liter bioreactor with appropriat作者: ODIUM 時(shí)間: 2025-3-29 12:39
Diverse effects of essential (,-6 and ,-3) fatty acids on cultured cells,ver, FAs have other effects on cells that may be less familiar. Polyunsaturated FAs of dietary origin (.-6 and .-3) cannot be synthesized by mammals, and are termed ‘essential’ because they are required for the optimal biologic function of specialized cells and tissues. However, they do not appear t作者: Glutinous 時(shí)間: 2025-3-29 16:45
Enhancement effects of BSA and linoleic acid on hybridoma cell growth and antibody production,c acid on its own promoted cell growth when used at concentrations below 50 mg LP., but strongly inhibited growth at a concentration of 100 mg L. on more. However, linoleic acid bound to bovine serum albumin did not inhibit cell growth, even at a concentration as high as 100 mg L.. Also, linoleic ac作者: 高談闊論 時(shí)間: 2025-3-29 22:31
Growth rate suppression of cultured mammalian cells enhances protein productivity,ion, was examined for enhancing protein production by cultured mammalian cells. Hybridoma 2E3 cells which were adapted to be interleukin-6 sensitively growth-suppressed accumulated the mRNA of IgG. which is reported stable, and IgG. production rate increased as a result when their growth was suppres作者: 舞蹈編排 時(shí)間: 2025-3-30 01:08 作者: 輕信 時(shí)間: 2025-3-30 07:30 作者: Devastate 時(shí)間: 2025-3-30 09:24
Adaptation of mammalian cells to non-ammoniagenic media,ted through storage or by cellular metabolism, leading to the formation of ammonia which can result in growth inhibition. Non-ammoniagenic alternatives to glutamine have been investigated in an attempt to develop strategies for obtaining improved cell yields for ammonia sensitive cell lines..Glutama作者: 背叛者 時(shí)間: 2025-3-30 16:11 作者: Veneer 時(shí)間: 2025-3-30 17:32
Extracellular insulin degrading activity creates instability in a CHO-based batch-refeed continuousl growth halted and cell viability dropped. This was found to be associated with depletion of insulin from the medium early during the affected passage. Insulin depletion was found to be primarily the result of insulin degrading activity released by the cells during the preceding passage.作者: 豐富 時(shí)間: 2025-3-30 21:59 作者: 財(cái)主 時(shí)間: 2025-3-31 00:56 作者: 食料 時(shí)間: 2025-3-31 05:35 作者: Offbeat 時(shí)間: 2025-3-31 09:25
Recombinant protein expression in a , cell line: comparison with the baculovirus system,t S2 cell line is simple and quick, and in batch fermentations the cells have a doubling time of 20 hours until reaching a plateau density of 20 million cells/ml. Protein expression is driven by the . Metaliothionein promoter which is tightly regulated. When expressed in S2 cells, the extracellular 作者: debouch 時(shí)間: 2025-3-31 15:27
Scale-up of the adenovirus expression system for the production of recombinant protein in human 293new constructions of adenovirus vectors, yielded as much as 10 to 20% recombinant protein with respect to the total cellular protein content. Until recently, high specific productivity of recombinant protein was limited to low cell density infected cultures of no more than 5 × 10. cells/mL. In this 作者: Innovative 時(shí)間: 2025-3-31 17:44 作者: 男學(xué)院 時(shí)間: 2025-4-1 00:19 作者: 熒光 時(shí)間: 2025-4-1 03:23 作者: 攤位 時(shí)間: 2025-4-1 06:52
Applications of improved stoichiometric model in medium design and fed-batch cultivation of animal by 8-fold compared to fed-batch culture in T-flask using the previous stoichiometric model. Ammonia formation was also decreased compared with both the batch and fed-batch cultures. Most importantly, the monoclonal antibody concentration reached 900 mg l., an increase of 17- and 1.6-fold compared wi作者: 喚起 時(shí)間: 2025-4-1 11:23 作者: FLIC 時(shí)間: 2025-4-1 17:59
