標題: Titlebook: Caveolae; Methods and Protocol Cedric M. Blouin Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 electron micro [打印本頁] 作者: 涌出 時間: 2025-3-21 17:04
書目名稱Caveolae影響因子(影響力)
書目名稱Caveolae影響因子(影響力)學科排名
書目名稱Caveolae網(wǎng)絡公開度
書目名稱Caveolae網(wǎng)絡公開度學科排名
書目名稱Caveolae被引頻次
書目名稱Caveolae被引頻次學科排名
書目名稱Caveolae年度引用
書目名稱Caveolae年度引用學科排名
書目名稱Caveolae讀者反饋
書目名稱Caveolae讀者反饋學科排名
作者: FIG 時間: 2025-3-21 23:48 作者: 憲法沒有 時間: 2025-3-22 03:08 作者: 戲法 時間: 2025-3-22 07:40
Book 2020e laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and comprehensive, .Caveolae: Methods and Protocols. is a valuable resource for both novice and expert researchers who are interested in discovering new roles or regulations of formed caveolae, and protein作者: left-ventricle 時間: 2025-3-22 09:31 作者: Aviary 時間: 2025-3-22 13:49
Bezugsrahmen und Hypothesenbildung,t of the plasma membrane. From these partitions, we estimate the relative fraction of caveolin that is punctate versus diffuse. Finally, we analyze the mobility of caveolin aggregates by tracking them and classify individual trajectories as diffusive or subdiffusive using a moment scaling spectrum a作者: Aviary 時間: 2025-3-22 18:54 作者: quiet-sleep 時間: 2025-3-22 22:12 作者: 的’ 時間: 2025-3-23 02:57 作者: 慌張 時間: 2025-3-23 05:46
978-1-0716-0734-3Springer Science+Business Media, LLC, part of Springer Nature 2020作者: 護身符 時間: 2025-3-23 12:01
Caveolae978-1-0716-0732-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 使虛弱 時間: 2025-3-23 14:17 作者: 啞劇 時間: 2025-3-23 21:18 作者: MERIT 時間: 2025-3-23 22:14
Overall Discussion and Conclusion,, transmission electron microscopy (TEM) has been the method of choice to study caveolae formation and ultrastructure and, more recently, to resolve the sub-caveolar localization of its protein components using novel protein labeling methods for TEM. This chapter describes a protocol for the selecti作者: 開始從未 時間: 2025-3-24 05:44 作者: 流浪 時間: 2025-3-24 09:05
https://doi.org/10.1007/978-3-8349-4216-6 cell surface, and that Caveolin-1 (CAV1) plays important roles in regulating ciliary membrane composition and function. Here we describe methods to analyze the localization and function of CAV1 in primary cilia of cultured mammalian cells. These include methods for culturing and transfecting mammal作者: 偽書 時間: 2025-3-24 12:16 作者: 暫時中止 時間: 2025-3-24 15:45 作者: 格子架 時間: 2025-3-24 19:02
Organisatorischer Wandel und Lernen,a membrane. Total internal reflection fluorescence microscopy exclusively illuminates molecules in the close vicinity of the glass surface, thereby reducing background fluorescence and enabling observation of the plasma membrane in the glass-attached cells with a high signal-to-noise ratio. Here, we作者: 小蟲 時間: 2025-3-24 23:41
https://doi.org/10.1007/978-3-8349-4485-6mple of a noninvasive technique that can be used to achieve this goal at nanometer resolution. FRET-based assays are dependent on the presence of fluorescent probes, such as CFP- and YFP-conjugated protein pairs. Here, we describe an experimental protocol in which live-cell FRET was used to measure 作者: 乳白光 時間: 2025-3-25 04:49
https://doi.org/10.1007/978-3-8349-4487-0proteins are known to interact with a variety of effector molecules, including G-protein-coupled receptors, Src family kinases, ion channels, endothelial nitric oxide synthase (eNOS), adenylyl cyclases, protein kinase A (PKA), and mitogen-activated PKs (MAPKs). There is, however, speculation on the 作者: accomplishment 時間: 2025-3-25 08:11 作者: 宣稱 時間: 2025-3-25 15:42 作者: 挑剔小責 時間: 2025-3-25 19:54
A Framework for Open Evaluationlae at the cell surface via coat proteins. The liposome co-sedimentation assay has been widely used for studies of protein and lipid interactions and has provided important information about binding mechanisms, lipid-binding specificity, and curvature preference of proteins. Here, we describe this t作者: 賄賂 時間: 2025-3-25 23:26 作者: Leaven 時間: 2025-3-26 00:21
https://doi.org/10.1007/978-3-8349-6165-5-stresses on the cell, endocytosis, and most importantly caveolae formation. As a consequence, there is intense interest in characterizing caveolin-1 structurally. Out of the many available structural techniques, nuclear magnetic resonance (NMR) spectroscopy is particularly well suited to investigat作者: squander 時間: 2025-3-26 06:59
Innovation and the Open Innovation concept,l as cells deleted of endogenous caveolar components. As one example, we will describe tagging of EHD2, caveolar neck protein, with Green Fluorescent protein (eGFP) from endogenous loci (knock-in, KI). As another example, we will describe deletion (knock-out, KO) of Caveolin1 (Cav1), an essential ca作者: 橫條 時間: 2025-3-26 09:53 作者: creditor 時間: 2025-3-26 12:42
Choice Situation in Low-Cost Marketssing the zebrafish notochord as a manipulable experimental system. Here, the methodologies to prepare, label, and simultaneously induce and image mechanical loading on live zebrafish notochord cells via electrical stimulation are described. This approach investigates membrane mechanics in a live, ph作者: 贊成你 時間: 2025-3-26 19:32
Operationalizing Dynamic Pricing Modelsen developed to study caveolae-dependent mechanoprotection and had to be adapted to the tissue or cells studied, as these structures are found in almost every type of cells. This chapter focuses on a protocol combining the use of live-cell imaging, micropatterning, hypo-osmotic shock as a mechanical作者: indifferent 時間: 2025-3-26 23:59
https://doi.org/10.1007/978-3-8349-6184-6tients suffering from neuromuscular diseases such as “Caveolinopathies” which are caused by mutations in the . gene encoding for caveolin-3. Human caveolin-3 is a 151 amino acid sized transmembrane protein localized within caveolae, predominantly expressed in cardiac and skeletal muscle cells and in作者: 免費 時間: 2025-3-27 03:48
Selective Visualization of Caveolae by TEM Using APEX2,, transmission electron microscopy (TEM) has been the method of choice to study caveolae formation and ultrastructure and, more recently, to resolve the sub-caveolar localization of its protein components using novel protein labeling methods for TEM. This chapter describes a protocol for the selecti作者: BARGE 時間: 2025-3-27 08:25
Freeze-Fracture Replica Immunolabeling of Cryopreserved Membrane Compartments, Cultured Cells and Tfunction involving membrane compartments. Freeze-fracturing of biological membranes offers both stunning views onto integral membrane proteins and perpendicular views over wide areas of the membrane at electron microscopical resolution. This information is directly assessable for 3D analyses and qua作者: Myocarditis 時間: 2025-3-27 10:25 作者: Metamorphosis 時間: 2025-3-27 15:07
Method for Efficient Observation of Caveolin-1 in Plasma Membrane by Microscopy Imaging Analysis,immunofluorescence microscopy, caveolin-1 (CAV1) is visualized as numerous small dots, which are often distributed as a linear array or along the edge of the cell. Although its presence, as well as that of other proteins, can be detected by conventional immunofluorescence microscopy, those results d作者: COMA 時間: 2025-3-27 21:19
Quantitative Image Analysis of the Spatial Organization and Mobility of Caveolin Aggregates at the omers that further assemble into higher-order structures. Imaging fluorescently labeled caveolin at the plasma membrane with total internal reflection fluorescence (TIRF) microscopy reveals a spatially heterogeneous distribution with aggregates of various sizes. In this chapter, we present a set of 作者: MURAL 時間: 2025-3-28 01:11
Spatiotemporal Analysis of Caveolae Dynamics Using Total Internal Reflection Fluorescence Microscopa membrane. Total internal reflection fluorescence microscopy exclusively illuminates molecules in the close vicinity of the glass surface, thereby reducing background fluorescence and enabling observation of the plasma membrane in the glass-attached cells with a high signal-to-noise ratio. Here, we作者: 半身雕像 時間: 2025-3-28 04:59
Live-Cell FRET Imaging of Phosphorylation-Dependent Caveolin-1 Switch,mple of a noninvasive technique that can be used to achieve this goal at nanometer resolution. FRET-based assays are dependent on the presence of fluorescent probes, such as CFP- and YFP-conjugated protein pairs. Here, we describe an experimental protocol in which live-cell FRET was used to measure 作者: explicit 時間: 2025-3-28 08:42 作者: 高原 時間: 2025-3-28 12:20
,Biotin Proximity Labeling to Identify Protein–Protein Interactions for Cavin1,ral cellular environment. Here I describe the use of BioID in HeLa cells to identify proteins that can potentially interact with cavin1, one of the main components of caveolae. Briefly, the method consists in the transfection of the cells with the fusion constructs containing the promiscuous biotin 作者: textile 時間: 2025-3-28 16:39 作者: Engaged 時間: 2025-3-28 21:40
Analysis of Protein and Lipid Interactions Using Liposome Co-sedimentation Assays,lae at the cell surface via coat proteins. The liposome co-sedimentation assay has been widely used for studies of protein and lipid interactions and has provided important information about binding mechanisms, lipid-binding specificity, and curvature preference of proteins. Here, we describe this t作者: 乏味 時間: 2025-3-29 02:01 作者: CRANK 時間: 2025-3-29 06:41
Preparation of Caveolin-1 for NMR Spectroscopy Experiments,-stresses on the cell, endocytosis, and most importantly caveolae formation. As a consequence, there is intense interest in characterizing caveolin-1 structurally. Out of the many available structural techniques, nuclear magnetic resonance (NMR) spectroscopy is particularly well suited to investigat作者: 卷發(fā) 時間: 2025-3-29 09:11 作者: 大酒杯 時間: 2025-3-29 11:58
Pulling of Tethers from the Cell Plasma Membrane Using Optical Tweezers,easuring the force required to hold the membrane tether at a constant length, which is related to the cell membrane tension. Following the evolution of this force during mechanical or chemical perturbations of the cell gives insight about the regulation of cell membrane tension. By pulling very long作者: AVOID 時間: 2025-3-29 17:44
Live Confocal Imaging of Zebrafish Notochord Cells Under Mechanical Stress In Vivo,sing the zebrafish notochord as a manipulable experimental system. Here, the methodologies to prepare, label, and simultaneously induce and image mechanical loading on live zebrafish notochord cells via electrical stimulation are described. This approach investigates membrane mechanics in a live, ph作者: Silent-Ischemia 時間: 2025-3-29 22:11
Study of Caveolae-Dependent Mechanoprotection in Human Muscle Cells Using Micropatterning and Live-en developed to study caveolae-dependent mechanoprotection and had to be adapted to the tissue or cells studied, as these structures are found in almost every type of cells. This chapter focuses on a protocol combining the use of live-cell imaging, micropatterning, hypo-osmotic shock as a mechanical作者: LEER 時間: 2025-3-30 00:26 作者: Abutment 時間: 2025-3-30 06:57 作者: rectum 時間: 2025-3-30 10:28 作者: fetter 時間: 2025-3-30 16:13 作者: 才能 時間: 2025-3-30 17:53 作者: 金絲雀 時間: 2025-3-30 23:04
,Investigation of Novel Cavin-1/Suppressor of Cytokine?Signaling 3 (SOCS3) Interactions by Coimmunopon with a discrete motif within the SOCS3 SH2 domain. Here, we describe in detail three methods (coimmunoprecipitation; peptide pull-down; peptide array overlay) we have used to validate and characterize cavin-1/SOCS3 interactions in vitro.作者: 顯而易見 時間: 2025-3-31 03:06
https://doi.org/10.1007/978-3-8349-4216-6ian cells with a CAV1-encoding plasmid or small interfering RNA (siRNA), analysis of mammalian cells by immunofluorescence microscopy (IFM) with antibodies against ciliary markers and CAV1, as well as methods for analyzing ciliary CAV1 function in siRNA-treated cells by IFM and cell-based signaling assays.作者: antidote 時間: 2025-3-31 07:04 作者: 使成核 時間: 2025-3-31 13:16 作者: BUMP 時間: 2025-3-31 15:12
Discrete Customer Choice Analysis membrane tethers, one can also probe the membrane reservoir of a cell and a sudden rise in the tether force is usually due to the depletion of excess membranes stored in membrane folds or invaginations.作者: MOT 時間: 2025-3-31 17:48
Choice Situation in Low-Cost Marketsysiological setting and is thus suited for caveola research where observations within the tissues of an intact organism are increasingly relevant. This chapter also aims to introduce fundamental methodologies for the use of zebrafish in “in vivo cell biology.”作者: Coeval 時間: 2025-4-1 00:16
Operationalizing Dynamic Pricing Models stress, and dyes such as calcein-AM and propidium iodide. We used this protocol for the in vitro study of the effect of mechanical stress on membrane integrity in human muscle cells from patients bearing caveolin-3 mutations.作者: Estimable 時間: 2025-4-1 03:19
Zum Member Relationship Marketing,be an unroofing procedure that clearly reveals CAV1 localization in a single plane of the plasma membrane and also demonstrate a super-resolution structured illumination microscopy technique for observation of CAV1 in the plasma membrane.作者: strain 時間: 2025-4-1 06:13
The Design Elements of Open Evaluationavin to chemically defined liposomes. The cavin proteoliposomes can be further analyzed to gain insights into lipid binding specificity, membrane-remodeling properties, and structural characteristics of the cavin family members.作者: 不要不誠實 時間: 2025-4-1 14:13 作者: 亂砍 時間: 2025-4-1 14:53 作者: heterogeneous 時間: 2025-4-1 19:37
Method for Efficient Observation of Caveolin-1 in Plasma Membrane by Microscopy Imaging Analysis,be an unroofing procedure that clearly reveals CAV1 localization in a single plane of the plasma membrane and also demonstrate a super-resolution structured illumination microscopy technique for observation of CAV1 in the plasma membrane.作者: Increment 時間: 2025-4-1 22:54
Liposome Binding Assay to Characterize the Structure and Function of Cavin Proteins,avin to chemically defined liposomes. The cavin proteoliposomes can be further analyzed to gain insights into lipid binding specificity, membrane-remodeling properties, and structural characteristics of the cavin family members.
