標(biāo)題: Titlebook: Cartilage and Osteoarthritis; Massimo Sabatini,Philippe Pastoureau,Frédéric Ceun Book 2004 Humana Press 2004 [打印本頁] 作者: dilate 時(shí)間: 2025-3-21 19:58
書目名稱Cartilage and Osteoarthritis影響因子(影響力)
書目名稱Cartilage and Osteoarthritis影響因子(影響力)學(xué)科排名
書目名稱Cartilage and Osteoarthritis網(wǎng)絡(luò)公開度
書目名稱Cartilage and Osteoarthritis網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cartilage and Osteoarthritis被引頻次
書目名稱Cartilage and Osteoarthritis被引頻次學(xué)科排名
書目名稱Cartilage and Osteoarthritis年度引用
書目名稱Cartilage and Osteoarthritis年度引用學(xué)科排名
書目名稱Cartilage and Osteoarthritis讀者反饋
書目名稱Cartilage and Osteoarthritis讀者反饋學(xué)科排名
作者: 著名 時(shí)間: 2025-3-21 22:28 作者: acrophobia 時(shí)間: 2025-3-22 02:28
The Identity, Dynamics, and Diffusion of MIS and accuracy in terms of determination of the amount of cDNA molecules. However, this method cannot account for factors such as efficiency of RNA isolation and reverse transcription conditions. Thus, normalization of the acquired data is required, with all its limitations as described.作者: Ballerina 時(shí)間: 2025-3-22 08:09
Lene Juel Rasmussen,Keshav K. Singh not limited to DNA but that essentially any molecule can be introduced into the cell using this approach. In addition to the primary protocol, we present two additional reliable, albeit less efficient backup protocols, the first using exponential decay electroporation and the second FuGENE? 6 trans作者: orient 時(shí)間: 2025-3-22 11:31 作者: inquisitive 時(shí)間: 2025-3-22 12:54 作者: inquisitive 時(shí)間: 2025-3-22 18:47 作者: 可用 時(shí)間: 2025-3-22 23:05 作者: 時(shí)間等 時(shí)間: 2025-3-23 04:23 作者: dithiolethione 時(shí)間: 2025-3-23 06:18
High-Efficiency Nonviral Transfection of Primary Chondrocytes, not limited to DNA but that essentially any molecule can be introduced into the cell using this approach. In addition to the primary protocol, we present two additional reliable, albeit less efficient backup protocols, the first using exponential decay electroporation and the second FuGENE? 6 trans作者: 昆蟲 時(shí)間: 2025-3-23 12:37
Analysis of Chondrocyte Functional Markers and Pericellular Matrix Components by Flow Cytometry,ays considered include the insulin-like growth factor-1 (IGF-1)/IGF receptor I (IGFRI), and the transforming growth factor-β1 (TGF-β1)/TGF-β receptor II (TGF-βRII) cascades, as well as the interleukin-1α/β (IL-1α/β)/interleukin-1 receptors I and II (IL-1RI and II) systems. Catabolic enzymes that med作者: CRAB 時(shí)間: 2025-3-23 15:17
A Simple and Reliable Assay of Proteoglycan Synthesis by Cultured Chondrocytes,hondrocytes, total proteoglycan synthesis (SP plus CAP) was increased 3.5-fold after the addition of 1.34 n. insulin-like growth factor-1 (IGF-1) compared with nonstimulated cells. Further addition of a fourfold molar ratio of IGF binding protein-3 completely abolished this effect. This method can b作者: degradation 時(shí)間: 2025-3-23 18:04 作者: LURE 時(shí)間: 2025-3-24 02:14 作者: Abominate 時(shí)間: 2025-3-24 05:59
Molecular Biology Intelligence Units only one cell type in both normal and diseased conditions. However, care has to be taken not to over- and misinterpret results, and some major challenges must be overcome in order to utilize the potential of this technology properly in the field of osteoarthritis.作者: GAVEL 時(shí)間: 2025-3-24 08:12 作者: 合唱隊(duì) 時(shí)間: 2025-3-24 14:05
Book 2004ularly in the weight-bearing joints. It has a stronger prevalence in women, and its incidence increases with age. OA is a major and growing health concern in developed countries, owing to steadily increasing life expectancy and the demand for better quality of life. Because of its chronic nature and作者: Inertia 時(shí)間: 2025-3-24 17:53
1543-1894 degeneration of articular cartilage, particularly in the weight-bearing joints. It has a stronger prevalence in women, and its incidence increases with age. OA is a major and growing health concern in developed countries, owing to steadily increasing life expectancy and the demand for better quality作者: perjury 時(shí)間: 2025-3-24 21:46 作者: 偶像 時(shí)間: 2025-3-25 00:11
Tom McMaster,David Wastell,Janice I. DeGross metalloproteinase and type II collagen gene expression from chondrocytes of human origin. Specific gene expression is revealed on an ethidium bromide-containing agarose gel on an ultraviolet plate and normalized to that of a housekeeping gene.作者: Arb853 時(shí)間: 2025-3-25 06:52
The Identity, Dynamics, and Diffusion of MISartilage-characteristic mRNAs. Probes labeled with this high-energy isotope provide good resolution at the tissue level with relatively low background; as a result of the probes that can be obtained that have a higher specificity to emulsion activity, very short exposure times are required.作者: 關(guān)心 時(shí)間: 2025-3-25 10:31
Distributed Microresonator Systems, are presented along with approaches for the characterization and affinity purification of the resulting antisera. Limitations associated with the use of antipeptide antibodies to study authentic protein neoepitopes are discussed.作者: 蕁麻 時(shí)間: 2025-3-25 14:38 作者: 亞麻制品 時(shí)間: 2025-3-25 19:44 作者: Hemoptysis 時(shí)間: 2025-3-25 21:49
Gene Expression Analysis in Cartilage by , Hybridization,artilage-characteristic mRNAs. Probes labeled with this high-energy isotope provide good resolution at the tissue level with relatively low background; as a result of the probes that can be obtained that have a higher specificity to emulsion activity, very short exposure times are required.作者: finale 時(shí)間: 2025-3-26 01:37
Production of Antibodies Against Degradative Neoepitopes in Aggrecan, are presented along with approaches for the characterization and affinity purification of the resulting antisera. Limitations associated with the use of antipeptide antibodies to study authentic protein neoepitopes are discussed.作者: 悠然 時(shí)間: 2025-3-26 06:07 作者: MAPLE 時(shí)間: 2025-3-26 11:18 作者: Predigest 時(shí)間: 2025-3-26 16:22
Fabrication Techniques for Microresonators,n, the use, and the effect of the transduction of chondrocytes or synovial fibroblasts with an adenoviral vector encoding the cDNA for glutamine: fructose-6-phosphate amidotransferase (GFAT). This is intended to serve as an example of a technology that can be used to evaluate the biological effects of overexpression of other cDNAs.作者: 金桌活畫面 時(shí)間: 2025-3-26 17:39 作者: 致命 時(shí)間: 2025-3-26 23:04 作者: Estrogen 時(shí)間: 2025-3-27 04:46 作者: Diluge 時(shí)間: 2025-3-27 09:11
In Vitro Gene Transfer to Chondrocytes and Synovial Fibroblasts by Adenoviral Vectors,n, the use, and the effect of the transduction of chondrocytes or synovial fibroblasts with an adenoviral vector encoding the cDNA for glutamine: fructose-6-phosphate amidotransferase (GFAT). This is intended to serve as an example of a technology that can be used to evaluate the biological effects of overexpression of other cDNAs.作者: Torrid 時(shí)間: 2025-3-27 12:38 作者: 脆弱帶來 時(shí)間: 2025-3-27 17:11
Davide Taibi,Luigi Lavazza,Sandro Morascan this way, chondrocytes can be recovered and further separated from the matrix by centrifugation. Almost all molecular and biochemical techniques, as well as a number of biological assays, are compatible with the culture of chondrocytes in alginate.作者: 粗糙 時(shí)間: 2025-3-27 20:42
https://doi.org/10.1007/978-0-387-72486-7g any immortalizing agent. Although immortalized chondrocytes should not be considered as substitutes for primary chondrocytes, they may be useful tools for evaluating and further validating mechanisms relevant to cartilage biology.作者: 空氣 時(shí)間: 2025-3-28 01:51
Culture of Chondrocytes in Alginate Beads,n this way, chondrocytes can be recovered and further separated from the matrix by centrifugation. Almost all molecular and biochemical techniques, as well as a number of biological assays, are compatible with the culture of chondrocytes in alginate.作者: Condescending 時(shí)間: 2025-3-28 04:03
Immortalization of Human Articular Chondrocytes for Generation of Stable, Differentiated Cell Linesg any immortalizing agent. Although immortalized chondrocytes should not be considered as substitutes for primary chondrocytes, they may be useful tools for evaluating and further validating mechanisms relevant to cartilage biology.作者: Assault 時(shí)間: 2025-3-28 06:52
Analysis of Differential Gene Expression in Healthy and Osteoarthritic Cartilage and Isolated Chonds only one cell type in both normal and diseased conditions. However, care has to be taken not to over- and misinterpret results, and some major challenges must be overcome in order to utilize the potential of this technology properly in the field of osteoarthritis.作者: 議程 時(shí)間: 2025-3-28 13:34
Changes of Chondrocyte Metabolism In Vitro,rypsin and matrix-assisted laser desorption ionization-time of flight mass spectrometry, using peptide mass fingerprinting. Tryptic peptide masses were measured and matched against a computer-generated list from the simulated trypsin proteolysis of a protein database (SwissProt).作者: 貪婪性 時(shí)間: 2025-3-28 15:46 作者: defray 時(shí)間: 2025-3-28 21:24 作者: 藐視 時(shí)間: 2025-3-28 23:16 作者: 沙文主義 時(shí)間: 2025-3-29 03:52 作者: Largess 時(shí)間: 2025-3-29 10:35
Davide Taibi,Luigi Lavazza,Sandro Morascacollagenase/dispase digestion and mixed with a solution of 1.25% alginic acid until a homogenous suspension is obtained. The suspension is drawn into a syringe and pushed gently through a needle, so that drops fall into a solution of calcium chloride. Beads form instantaneously and further polymeriz作者: 偽證 時(shí)間: 2025-3-29 14:59 作者: cancer 時(shí)間: 2025-3-29 18:11 作者: 文件夾 時(shí)間: 2025-3-29 19:55 作者: enumaerate 時(shí)間: 2025-3-30 02:55 作者: LVAD360 時(shí)間: 2025-3-30 06:25
The Identity, Dynamics, and Diffusion of MISsts working on cartilage, in terms of outstanding sensitivity and accuracy. Four approaches to determine mRNA expression levels by PCR amplification of specific cDNA sequences are currently in use and are discussed in this chapter: conventional PCR with end-point determination, conventional PCR in t作者: 出汗 時(shí)間: 2025-3-30 10:26
Peter M. Bednar,Christine Welchxtracellular matrix rich in proteoglycans, which copurify with the RNA as they are large and negatively charged macromolecules. In our laboratory, we are interested in searching for genes differentially expressed in chondrocytes in diverse in vivo situations, for instance during maturation of chondr作者: Synovial-Fluid 時(shí)間: 2025-3-30 14:51
The Identity, Dynamics, and Diffusion of MISrotocol using double-stranded DNA or single-stranded RNA probes labeled with [.P] to localize and visualize the temporal and spatial distribution of cartilage-characteristic mRNAs. Probes labeled with this high-energy isotope provide good resolution at the tissue level with relatively low background作者: EWER 時(shí)間: 2025-3-30 20:08 作者: chondromalacia 時(shí)間: 2025-3-31 00:25
Lene Juel Rasmussen,Keshav K. Singhr the advantage of relatively simple vector design, production, and purification and, for tissue engineering applications, avoid many of the potential risks associated with virus-mediated transfection methods. Unfortunately, primary cells, and in particular chondrocytes, are notoriously refractory t作者: CRATE 時(shí)間: 2025-3-31 04:45 作者: Credence 時(shí)間: 2025-3-31 05:21 作者: conference 時(shí)間: 2025-3-31 09:42 作者: Focus-Words 時(shí)間: 2025-3-31 16:42 作者: emulsify 時(shí)間: 2025-3-31 19:55 作者: 無脊椎 時(shí)間: 2025-4-1 00:10
Distributed Microresonator Systems,eolytic cleavage sites in aggrecan is described. Principles of peptide design and methods for preparation and characterization of ovalbumin conjugates are presented along with approaches for the characterization and affinity purification of the resulting antisera. Limitations associated with the use作者: Myocarditis 時(shí)間: 2025-4-1 04:02
Springer Series in Optical Sciencesome of these assays and their applications are described in this chapter. These and other assays are commercially available. The applications of these assays are discussed with examples from recent publications.作者: STYX 時(shí)間: 2025-4-1 07:25
Springer Series in Optical Sciences chondrocytes, this chapter describes complementary techniques that may be used in combination. During apoptotic death, protein and DNA breakdown is accomplished by caspases (cysteine proteases) in a highly regulated manner. Activation of caspases occurs in the initiation and/or the execution phase 作者: ostracize 時(shí)間: 2025-4-1 13:30 作者: Hallowed 時(shí)間: 2025-4-1 16:20
Methods in Molecular Medicinehttp://image.papertrans.cn/c/image/222209.jpg
