標(biāo)題: Titlebook: Cartilage Tissue Engineering; Methods and Protocol Pauline M. Doran Book 2015 Springer Science+Business Media New York 2015 Bioreactors.Car [打印本頁] 作者: Disperse 時間: 2025-3-21 18:09
書目名稱Cartilage Tissue Engineering影響因子(影響力)
作者: Peak-Bone-Mass 時間: 2025-3-21 22:34 作者: 袖章 時間: 2025-3-22 01:57 作者: 原始 時間: 2025-3-22 07:46 作者: 脫離 時間: 2025-3-22 10:48 作者: Eulogy 時間: 2025-3-22 13:40 作者: Eulogy 時間: 2025-3-22 18:58 作者: LURE 時間: 2025-3-22 23:07
Fourier Transformation and FT-Spectroscopy,uch of our practical experience with three-dimensional cultures, tissue bioreactors, scaffold materials, stem cells, and differentiation protocols was gained using cartilage as a model system. Despite these advances, however, generation of engineered cartilage matrix with the composition, structure,作者: inquisitive 時間: 2025-3-23 02:34 作者: 悲痛 時間: 2025-3-23 06:26
https://doi.org/10.1007/978-0-387-69492-4ability and multipotent character means that mesenchymal stem cells have become a focus for the field of tissue engineering, particularly for the repair of bone and cartilage..This chapter describes the isolation of mesenchymal stem cells from human bone marrow tissue, as well as expansion of the ce作者: Obsessed 時間: 2025-3-23 10:25 作者: 多骨 時間: 2025-3-23 14:17
Maxwell II. Modes and Mode Propagation,directed differentiation of human embryonic stem (hES) cells to the chondrogenic lineage. The initial enrichment of a hemangioblast intermediate has been shown to yield a replenishable population of highly purified progenitor cells that exhibit the typical mesenchymal stem cell (MSC) surface markers作者: 公社 時間: 2025-3-23 21:18
Maxwell II. Modes and Mode Propagation, patient-specific cells for the regeneration of skeletal tissue, such as articular cartilage. Devising efficient and reproducible strategies, which closely mimic the physiological chondrogenic differentiation process, will be necessary to generate functional chondrocytes from human iPS cells. Our pr作者: PET-scan 時間: 2025-3-23 23:22 作者: nutrients 時間: 2025-3-24 02:28 作者: 滑稽 時間: 2025-3-24 06:33 作者: 其他 時間: 2025-3-24 10:58 作者: 無表情 時間: 2025-3-24 15:03 作者: 松果 時間: 2025-3-24 19:27
Gunther Spohn,Martin F. Bachmannuct for cartilage repair should provide a support for the cell and allow sustained in situ delivery of bioactive factors capable of inducing cell differentiation into chondrocytes. Pharmacologically active microcarriers (PAMs), made of biodegradable and biocompatible poly (.,.-lactide-co-glycolide a作者: Cerumen 時間: 2025-3-25 02:31
Sanjay K. Agarwal MD,R. Jeffrey Chang MDedicine. Here, we describe the production of hierarchical PMCs composed by an external multilayered membrane by layer-by-layer assembly of poly(.-lysine), alginate, and chitosan. The core of the PMCs is liquified and encapsulates human adipose stem cells and surface-functionalized collagen II-TGF-β3作者: 放牧 時間: 2025-3-25 03:35
Bioengineering and the Ovarian Follicleaterials are chosen to fulfill specific features required to mimic the complex osteochondral tissue interface, including cartilage, interlayer tissue, and subchondral bone. Here, the biomaterials and fabrication methods currently used to manufacture stratified multilayered scaffolds as well as cell 作者: 愛國者 時間: 2025-3-25 08:14
Steven F. Mullen PhD,John K. Critser PhD cartilage constructs. In this chapter, we describe valuable methods of imposing direct mechanical stimuli (compression or shear) to tissue-engineered cartilage constructs as well as some common analytical methods used to quantify the effects of mechanical stimuli after short-term or long-term loadi作者: myopia 時間: 2025-3-25 12:10
Cartilage Tissue Engineering978-1-4939-2938-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 沉積物 時間: 2025-3-25 18:05 作者: 壯麗的去 時間: 2025-3-25 19:59
https://doi.org/10.1007/978-0-387-69900-4d mechanical properties. This chapter also presents the methods to characterize these tissue engineered constructs. The resulting tissue engineered constructs can be useful for the generation of cartilage tissue both in vitro and in vivo.作者: 相同 時間: 2025-3-26 00:58
Steven F. Mullen PhD,John K. Critser PhD cartilage constructs. In this chapter, we describe valuable methods of imposing direct mechanical stimuli (compression or shear) to tissue-engineered cartilage constructs as well as some common analytical methods used to quantify the effects of mechanical stimuli after short-term or long-term loading.作者: gorgeous 時間: 2025-3-26 04:45
1064-3745 ation advice from the experts.This volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; differentiation; synthesis and application of three-dimensional scaffol作者: Intrepid 時間: 2025-3-26 11:48 作者: Cougar 時間: 2025-3-26 16:11 作者: BIDE 時間: 2025-3-26 18:31
Sanjay K. Agarwal MD,R. Jeffrey Chang MDne), alginate, and chitosan. The core of the PMCs is liquified and encapsulates human adipose stem cells and surface-functionalized collagen II-TGF-β3 poly(.-lactic acid) microparticles for cartilage tissue engineering.作者: 雪崩 時間: 2025-3-26 23:46
Bioengineering and the Ovarian Follicle and subchondral bone. Here, the biomaterials and fabrication methods currently used to manufacture stratified multilayered scaffolds as well as cell seeding techniques for their characterization are presented.作者: 巡回 時間: 2025-3-27 03:26
Human Fetal and Adult Chondrocytesngineered tissues. In this chapter, procedures are outlined for isolation of chondrocytes from human fetal and adult cartilage. Methods for expansion and cryopreservation of the cells and characterization of gene expression using quantitative polymerase chain reaction (Q-PCR) analysis are also described.作者: 承認(rèn) 時間: 2025-3-27 06:39
Mesenchymal Stem Cells Derived from Human Adipose Tissueem cells from human adult fat tissue, propagate the cells in culture, and cryopreserve the cells for tissue engineering applications. Flow cytometry methods are also described for identification and characterization of adipose-derived stem cells and for cell sorting.作者: TAIN 時間: 2025-3-27 12:18
Nanostructured Capsules for Cartilage Tissue Engineeringne), alginate, and chitosan. The core of the PMCs is liquified and encapsulates human adipose stem cells and surface-functionalized collagen II-TGF-β3 poly(.-lactic acid) microparticles for cartilage tissue engineering.作者: maladorit 時間: 2025-3-27 17:33 作者: 毛細(xì)血管 時間: 2025-3-27 21:29
Book 2015ion and expansion of chondrocytes and stem cells; differentiation; synthesis and application of three-dimensional scaffolds; design and operation of bioreactors; .in vivo. testing of engineered constructs; and molecular and functional analysis of cartilage cells and tissues. Frequently used technolo作者: faddish 時間: 2025-3-27 22:58
Fourier Transformation and FT-Spectroscopy,er construct implantation. The aim of this chapter is to distil from the large available body of literature the seminal approaches and experimental techniques developed for cartilage tissue engineering and to identify those specific areas requiring further research effort.作者: 并排上下 時間: 2025-3-28 02:07
Maxwell II. Modes and Mode Propagation,howed temporal expression of cartilage genes and the accumulation of a cartilaginous extracellular matrix in vitro. In this chapter, we provide detailed methodologies for the differentiation of human iPS cells to the chondrogenic lineage and describe protocols for the analysis of chondrogenic differentiation.作者: 遺忘 時間: 2025-3-28 10:04
Cartilage Tissue Engineering: What Have We Learned in Practice?er construct implantation. The aim of this chapter is to distil from the large available body of literature the seminal approaches and experimental techniques developed for cartilage tissue engineering and to identify those specific areas requiring further research effort.作者: Chandelier 時間: 2025-3-28 12:50
Differentiation of Human Induced Pluripotent Stem Cells to Chondrocyteshowed temporal expression of cartilage genes and the accumulation of a cartilaginous extracellular matrix in vitro. In this chapter, we provide detailed methodologies for the differentiation of human iPS cells to the chondrogenic lineage and describe protocols for the analysis of chondrogenic differentiation.作者: 植物群 時間: 2025-3-28 16:54 作者: 堅毅 時間: 2025-3-28 22:04 作者: 硬化 時間: 2025-3-29 00:15 作者: MEAN 時間: 2025-3-29 05:51
Triptolide Alters Mitochondrial Functions,ix. Hence, we employed gelatin microspheres as porogens to create a microcavitary alginate hydrogel as an interim scaffold to facilitate initial chondrocyte 3D culture and to establish a final scaffold-free living hyaline cartilaginous graft (LhCG) for cartilage tissue engineering.作者: 名義上 時間: 2025-3-29 07:43 作者: 讓步 時間: 2025-3-29 14:45 作者: 樹木中 時間: 2025-3-29 17:26 作者: 阻撓 時間: 2025-3-29 21:33 作者: cuticle 時間: 2025-3-30 03:24
Bioprinted Scaffolds for Cartilage Tissue Engineeringaintenance and load bearing, while the hydrogel provides the biological environment for artificial cartilage regeneration. This chapter introduces the procedures for printing of a 3-D scaffold using our hybrid bioprinting technology and includes the source materials for preparation of 3-D printing.作者: 明確 時間: 2025-3-30 06:11
Scaffolds for Controlled Release of Cartilage Growth Factorsoteins encapsulated in polymeric microspheres with a solid in oil in water emulsion-solvent evaporation process, and their subsequent coating with extracellular matrix protein molecules. Here, we describe their preparation process, and some of their characterization methods for an application in cartilage tissue engineering.作者: abduction 時間: 2025-3-30 11:32
Maxwell II. Modes and Mode Propagation, as well as the capacity for multilineage differentiation to bone, fat, and cartilage. Herein, we provide detailed methodologies for the derivation and characterization of potent mesenchymal-like progenitors from hES cells and describe in vitro assays for bone morphogenetic protein (BMP)-2-mediated differentiation to the chondrogenic lineage.作者: badinage 時間: 2025-3-30 12:28
Ontology Management Infrastructuresng scanning electron microscope and Picrosirius Red staining respectively. Methods to evaluate the metabolic activity and proliferation of cells (resazurin-based assay and DNA assay, respectively) and gene expression are also presented. Furthermore, histological techniques to analyze the presence of sulfated glycosaminoglycans are also described.作者: 欺騙手段 時間: 2025-3-30 20:13 作者: 解脫 時間: 2025-3-30 22:28
Human Fetal and Adult ChondrocytesHuman adult, juvenile, neonatal, and fetal chondrocytes have all been demonstrated to produce cartilage matrix components in vitro for production of engineered tissues. In this chapter, procedures are outlined for isolation of chondrocytes from human fetal and adult cartilage. Methods for expansion 作者: patriot 時間: 2025-3-31 04:46
Mesenchymal Stem Cells Derived from Human Bone Marrowability and multipotent character means that mesenchymal stem cells have become a focus for the field of tissue engineering, particularly for the repair of bone and cartilage..This chapter describes the isolation of mesenchymal stem cells from human bone marrow tissue, as well as expansion of the ce作者: 詞匯記憶方法 時間: 2025-3-31 08:45
Mesenchymal Stem Cells Derived from Human Adipose Tissuepacity of mesenchymal stem cells makes them very valuable for cell-based medical therapies. In this chapter, we describe how to isolate mesenchymal stem cells from human adult fat tissue, propagate the cells in culture, and cryopreserve the cells for tissue engineering applications. Flow cytometry m作者: 嘲弄 時間: 2025-3-31 09:49
Derivation and Chondrogenic Commitment of Human Embryonic Stem Cell-Derived Mesenchymal Progenitorsdirected differentiation of human embryonic stem (hES) cells to the chondrogenic lineage. The initial enrichment of a hemangioblast intermediate has been shown to yield a replenishable population of highly purified progenitor cells that exhibit the typical mesenchymal stem cell (MSC) surface markers
