標題: Titlebook: Cartilage Tissue Engineering; Martin J. Stoddart,Elena Della Bella,Angela R. Arm Book 2023 The Editor(s) (if applicable) and The Author(s) [打印本頁] 作者: magnify 時間: 2025-3-21 18:15
書目名稱Cartilage Tissue Engineering影響因子(影響力)
作者: 錯 時間: 2025-3-21 20:38
Chondrocyte Isolation and Expansion,acted primary chondrocytes will then adhere to standard tissue culture plastics, typically in small clusters, over a period of a few days in monolayer culture. Chondrocyte populations are expanded in a basal medium containing serum, supplemented with ascorbic acid, antibiotics, and sometimes antifun作者: 喃喃訴苦 時間: 2025-3-22 03:40 作者: 飛來飛去真休 時間: 2025-3-22 04:36
Articular Cartilage Chondroprogenitors: Isolation and Directed Differentiation, maintenance, and repair. In this chapter, we show how colony-forming progenitor-like cells can be isolated from bovine articular cartilage using differential adhesion to fibronectin. Furthermore, we describe the optimal conditions and factors required to differentiate these progenitor cells to prod作者: Infiltrate 時間: 2025-3-22 09:54
Physioxic Culture of Chondrogenic Cells, between 2% and 5% oxygen. Although the literature has historically termed this level of oxygen as hypoxia, particularly when doing experiments in vitro in this range, this is actually the physiological oxygen tension experienced in vivo and is more accurately termed physioxia. In general, culture o作者: 先行 時間: 2025-3-22 16:56 作者: 先行 時間: 2025-3-22 21:02 作者: enmesh 時間: 2025-3-22 23:46 作者: Ophthalmoscope 時間: 2025-3-23 05:07
Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering. This makes them ideal candidates for treating focal cartilage defects and cartilage degeneration in osteoarthritis (OA). Observational studies have reported beneficial biological effects of EVs, such as inhibition of inflammation, enhanced extracellular matrix deposition, and reduced cartilage d作者: 斷言 時間: 2025-3-23 06:48 作者: initiate 時間: 2025-3-23 12:40
Techniques for Visualization and Quantification of Primary Cilia in Chondrocytes,disease. Despite this, the chondrocyte primary cilium and its associated role in cartilage biology remains poorly understood. Key to elucidating primary cilia structure and function in chondrocytes is the ability to visualize this unique structure. Here we describe materials and methods for immunofl作者: Cumbersome 時間: 2025-3-23 15:29
Co-culture of Human Articular Chondrocytes Seeded in Polyurethane Scaffolds and Human Mesenchymal Sn indirect co-culture technique based on encapsulation of chondrocytes and mesenchymal stromal cells in polyurethane scaffolds and alginate beads, respectively. This way, both cell populations can communicate through paracrine effects in the?absence of cell-cell contact. Due to the mechanical proper作者: arboretum 時間: 2025-3-23 22:06 作者: Graphite 時間: 2025-3-24 01:33
Modulation of MicroRNA Expression During In Vitro Chondrogenesis, proliferation, apoptosis, metabolism, and differentiation. These small non-coding RNAs function by interacting with specific regions in the 3′-untranslated region of mRNAs, thereby resulting in mRNA degradation or suppression of translation. Since miRNAs have the ability to target many mRNAs within作者: 抵消 時間: 2025-3-24 02:42 作者: 使絕緣 時間: 2025-3-24 07:24
Histological Preparation and Evaluation of Cartilage Specimens,ed on the importance to investigate cartilage and bone as a unit, which includes the transition zone of the calcified cartilage and tidemark. Reasons for the appropriate selection of histological methods are presented such as when to use (decalcified) specimens for routine paraffin embedding includi作者: 金盤是高原 時間: 2025-3-24 12:47 作者: LUT 時間: 2025-3-24 15:33 作者: superfluous 時間: 2025-3-24 20:13 作者: THE 時間: 2025-3-25 00:19 作者: grovel 時間: 2025-3-25 05:20 作者: DEFT 時間: 2025-3-25 09:05 作者: pineal-gland 時間: 2025-3-25 15:39 作者: 衰老 時間: 2025-3-25 19:41
Chondrogenic Differentiation of Human-Induced Pluripotent Stem Cells,ion. The 6-week protocol results in hiPSC-derived cartilaginous tissue that can be characterized by histology, immunohistochemistry, and gene expression or enzymatically digested to isolate chondrocyte-like cells. Investigators can use this protocol for experiments including genetic engineering, in vitro disease modeling, or tissue engineering.作者: terazosin 時間: 2025-3-25 23:30
Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineeriolation of EVs from conditioned cell culture media or biofluids. In addition, three methods for characterization of isolated EVs are suggested based on physical properties, protein profiling, and ultrastructural morphology.作者: Obscure 時間: 2025-3-26 02:23
Histological Preparation and Evaluation of Cartilage Specimens,luding open communication about difficulties related to the various techniques, also practical instructions for state-of-the-art evaluation methods and their strengths and weaknesses are given. Sample figures for scoring methods are included.作者: custody 時間: 2025-3-26 05:49
Book 2023eshooting and avoiding known pitfalls.?.Authoritative and up-to-date, .Cartilage Tissue Engineering. serves as an ideal guide for researchers working to advance the vital study of cartilage biology and repair..作者: Detonate 時間: 2025-3-26 11:43 作者: 靈敏 時間: 2025-3-26 13:13
Simulating SSTM Using Monte Carlo, expansion of chondrocytes by the Oswestry/Keele University Orthopaedic Research (OsKOR) group and John Charnley GMP and MHRA licensed laboratory, both based at the RJAH Orthopaedic Hospital, Oswestry, UK.作者: 說笑 時間: 2025-3-26 19:24 作者: –LOUS 時間: 2025-3-27 00:57 作者: Priapism 時間: 2025-3-27 03:46
Isolation of Murine Articular Chondrocytes for Single-Cell RNA or Bulk RNA Sequencing Analysis,further single-cell analysis. This protocol highlights a successful technique optimized for isolating chondrocytes from the articulated joints of rodent animal models using a series of enzymatic digestions and chondrocyte enrichment using a double negative selection process through florescence-activated cell sorting (FACS).作者: LEVER 時間: 2025-3-27 06:17 作者: 身心疲憊 時間: 2025-3-27 11:47 作者: 煩憂 時間: 2025-3-27 16:14 作者: 鞠躬 時間: 2025-3-27 18:53 作者: WAX 時間: 2025-3-27 23:44
Isolation of Chondrons from Hyaline Cartilage,matrix. Therefore, this combination of enzymes can be used to enzymatically isolate chondrons from hyaline cartilage. Chondrons have a high potential for cartilage tissue engineering. This chapter describes in detail how chondrons can be isolated from hyaline cartilage for further use.作者: 孤僻 時間: 2025-3-28 02:52
Metabolomic Profiling to Understand Chondrocyte Metabolism, quantitative measurement of thousands of small molecule metabolites that serve as both products and reactants to myriad reactions of cellular biochemistry. This protocol describes procedures to perform metabolomic profiling on chondrocytes and other tissues and fluids within the synovial joint.作者: Gum-Disease 時間: 2025-3-28 09:41
Co-culture of Human Articular Chondrocytes Seeded in Polyurethane Scaffolds and Human Mesenchymal Sties of polyurethane, this model can be employed in mechanobiology studies. The resulting engineered cultures can provide a more realistic environment, recreating the complex joints’ microenvironment and physiology.作者: 不舒服 時間: 2025-3-28 10:40 作者: DEAF 時間: 2025-3-28 18:04 作者: Formidable 時間: 2025-3-28 20:39 作者: 免費 時間: 2025-3-29 02:25 作者: Vldl379 時間: 2025-3-29 04:42 作者: 圖畫文字 時間: 2025-3-29 09:40 作者: LAIR 時間: 2025-3-29 14:26
Maxwell II. Modes and Mode Propagation,m sample preparation to parameter identification. Examples from our ongoing experiments on alginate hydrogel constructs and preserved and damaged cartilage explants obtained from human hip samples are presented.作者: magnate 時間: 2025-3-29 19:11
Cartilage Tissue Engineering: An Introduction,reas that need further research. The concept of translation and the route to clinical translation must be kept in mind if some of the promising preclinical research is to make it to routine clinical application.作者: 暗指 時間: 2025-3-29 21:21
Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation, combined with cell filtration and followed by monolayer attachment and expansion culture. Quality control requires confirmation of correct surface marker expression and multilineage differentiation potential by a trilineage differentiation assay.作者: Badger 時間: 2025-3-30 03:22
Techniques for Visualization and Quantification of Primary Cilia in Chondrocytes,ry cilia structure and function in chondrocytes is the ability to visualize this unique structure. Here we describe materials and methods for immunofluorescence labeling, microscopy, and measurement of chondrocyte primary cilia.作者: 有說服力 時間: 2025-3-30 05:28
Purification and Isolation of Proteins from Hyaline Cartilage,proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage. Cartilage tissue engineering efforts rely on the comprehensive understanding of the composition of cartilage and the function of each of the protein constituents.作者: 一致性 時間: 2025-3-30 11:38
Unconfined Compression Experimental Protocol for Cartilage Explants and Hydrogel Constructs: From Sm sample preparation to parameter identification. Examples from our ongoing experiments on alginate hydrogel constructs and preserved and damaged cartilage explants obtained from human hip samples are presented.作者: 釘牢 時間: 2025-3-30 15:50
Modulation of MicroRNA Expression During In Vitro Chondrogenesis,o chondrogenesis of human cartilage progenitor cells (CPCs). Specifically, we describe how we obtain CPCs from human articular cartilage specimens, how we generate and titrate lentivirus engineered to overexpress a precursor miRNA, how we transduce CPCs with lentivirus and differentiate them toward 作者: 嫻熟 時間: 2025-3-30 20:06
https://doi.org/10.1007/978-0-387-68979-1o chondrogenesis of human cartilage progenitor cells (CPCs). Specifically, we describe how we obtain CPCs from human articular cartilage specimens, how we generate and titrate lentivirus engineered to overexpress a precursor miRNA, how we transduce CPCs with lentivirus and differentiate them toward 作者: macular-edema 時間: 2025-3-30 22:25 作者: JIBE 時間: 2025-3-31 04:43 作者: extinct 時間: 2025-3-31 08:23
The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulfated Glycosaminoglycans,The 1,9-dimethylmethylene blue (DMMB) assay enables the detection of sulfated glycosaminoglycans (sGAGs). This assay can be used to quickly quantify the sGAG content in a large number of samples using spectrophotometry. While this widespread assay appears straightforward, there are certain pitfalls that need to be considered.作者: 謊言 時間: 2025-3-31 11:26 作者: 不利 時間: 2025-3-31 16:25
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/222207.jpg作者: conflate 時間: 2025-3-31 19:50 作者: bioavailability 時間: 2025-4-1 01:08
Simulating SSTM Using Monte Carlo,acted primary chondrocytes will then adhere to standard tissue culture plastics, typically in small clusters, over a period of a few days in monolayer culture. Chondrocyte populations are expanded in a basal medium containing serum, supplemented with ascorbic acid, antibiotics, and sometimes antifun作者: 逃避系列單詞 時間: 2025-4-1 04:06 作者: 慎重 時間: 2025-4-1 09:18
Online Business Security Systems maintenance, and repair. In this chapter, we show how colony-forming progenitor-like cells can be isolated from bovine articular cartilage using differential adhesion to fibronectin. Furthermore, we describe the optimal conditions and factors required to differentiate these progenitor cells to prod作者: cogitate 時間: 2025-4-1 12:42
Online Business Security Systems between 2% and 5% oxygen. Although the literature has historically termed this level of oxygen as hypoxia, particularly when doing experiments in vitro in this range, this is actually the physiological oxygen tension experienced in vivo and is more accurately termed physioxia. In general, culture o作者: HEDGE 時間: 2025-4-1 16:17 作者: 全部逛商店 時間: 2025-4-1 22:23 作者: CHAFE 時間: 2025-4-1 23:05
Optical Scanning Holography: Applications,ies for cartilage regeneration and high-throughput drug screening. This protocol describes chondrogenic differentiation of human-induced pluripotent stem cells (hiPSCs), which can undergo genetic modification and the capacity for extensive cell expansion. The hiPSCs are differentiated in a stepwise 作者: 舊病復發(fā) 時間: 2025-4-2 03:56
