標題: Titlebook: Cardiac Gene Expression; Methods and Protocol Jun Zhang,Gregg Rokosh Book 2007 Humana Press 2007 DNA.In silico.Microarray.PCR.Polymerase Ch [打印本頁] 作者: Ingrown-Toenail 時間: 2025-3-21 18:16
書目名稱Cardiac Gene Expression影響因子(影響力)
書目名稱Cardiac Gene Expression影響因子(影響力)學科排名
書目名稱Cardiac Gene Expression網(wǎng)絡公開度
書目名稱Cardiac Gene Expression網(wǎng)絡公開度學科排名
書目名稱Cardiac Gene Expression被引頻次
書目名稱Cardiac Gene Expression被引頻次學科排名
書目名稱Cardiac Gene Expression年度引用
書目名稱Cardiac Gene Expression年度引用學科排名
書目名稱Cardiac Gene Expression讀者反饋
書目名稱Cardiac Gene Expression讀者反饋學科排名
作者: blight 時間: 2025-3-21 21:58 作者: 偽造者 時間: 2025-3-22 02:18 作者: Myosin 時間: 2025-3-22 08:02 作者: 狗窩 時間: 2025-3-22 09:07 作者: 原告 時間: 2025-3-22 15:39 作者: 原告 時間: 2025-3-22 18:17
Quantitative (Real-Time) RT-PCR in Cardiovascular Researchtation of nucleic acids. Owing to its large dynamic range and throughput, this approach has become the chosen method for rapid quantification of mRNA levels in biological samples. The sensitivity of this method permits the reliable detection of low concentrations of initial template, while deliverin作者: Immunization 時間: 2025-3-22 21:28 作者: 模范 時間: 2025-3-23 03:00
Hybridization rapid methodology provides precious insights into the complex gene organization and expression within an heterogeneous cell population. This technique is particularly useful to elucidate the genes and pathways involved in cardiac cells processes (differentiation, proliferation, apoptosis) or in the作者: 串通 時間: 2025-3-23 08:23
Characterization of ,-Regulatory Elements and Transcription Factor Binding gel mobility shift assay (GMSA) or gel retardation assay are essential and common steps. In addition to providing a general protocol for GMSA, this chapter describes some applications of this assay to characterize cardiac-specific and ubiquitous .-acting factors bound to regulatory elements [novel 作者: Mobile 時間: 2025-3-23 10:34 作者: 吃掉 時間: 2025-3-23 14:59
Characterization of Cardiac Gene Promoter Activityhanisms that regulate gene expression in the myocardium. Initial studies focused on the regulated expression of contractile genes, since their transcripts are abundant and their cDNAs were among the first to be cloned. More recent studies have focused on the promoters of genes expressed at much lowe作者: Obsessed 時間: 2025-3-23 19:28
Comparative Genomicsstraint. Although this has proved powerful for gene identification, significant progress has also been made in uncovering gene regulatory sequences such as distant acting transcriptional enhancers. These pursuits have led to the development of a variety of valuable databases and resources that shoul作者: 肉體 時間: 2025-3-23 23:33 作者: PLIC 時間: 2025-3-24 04:13 作者: 外形 時間: 2025-3-24 08:38
https://doi.org/10.1007/978-1-59745-030-0DNA; In silico; Microarray; PCR; Polymerase Chain Reaction; Promoter; SNP; Single Nucleotide Polymorphism; g作者: 侵略 時間: 2025-3-24 13:00
Jun Zhang,Gregg RokoshResources for the analysis of gene regulation data and SNPs.Comparative genomic approach for functionally annotating human DNA.Quantitative, real-time RT-PCR in cardiovascular research.In silico analy作者: induct 時間: 2025-3-24 17:18 作者: MARS 時間: 2025-3-24 21:24 作者: 帶來 時間: 2025-3-25 00:09 作者: 有節(jié)制 時間: 2025-3-25 06:08
Inequalities for mixing processes,paration and sequencing of cDNA concatemers, but it does not require prior knowledge of the genes to be assayed (as with microarrays). Once analyzed, SAGE data provide both a qualitative and quantitative assessment of potentially every transcript present in a particular cell or tissue type. In this 作者: 流眼淚 時間: 2025-3-25 10:23
https://doi.org/10.1007/978-1-4684-0489-0tractive suppression hybridization represents a large-scale, unbiased method for detecting transcriptionally and posttranscriptionally regulated genes, both known and unknown, independently of the prevalence of these transcripts. The strength of subtractive hybridization relies on its unbiased natur作者: 草本植物 時間: 2025-3-25 14:23 作者: Proponent 時間: 2025-3-25 18:27 作者: 發(fā)芽 時間: 2025-3-25 20:44 作者: amphibian 時間: 2025-3-26 00:32 作者: prediabetes 時間: 2025-3-26 07:56 作者: VALID 時間: 2025-3-26 11:43 作者: 蚊子 時間: 2025-3-26 15:06
The local time density estimator,analysis of components controlling its expression, namely, .-acting regulatory elements and their cognate binding proteins. It also enables assembly of meaningful reporter constructs to examine promoter function in different cellular contexts. In this chapter, basic protocols for two experimental ap作者: 推遲 時間: 2025-3-26 18:39
The local time density estimator,hanisms that regulate gene expression in the myocardium. Initial studies focused on the regulated expression of contractile genes, since their transcripts are abundant and their cDNAs were among the first to be cloned. More recent studies have focused on the promoters of genes expressed at much lowe作者: 含水層 時間: 2025-3-26 21:55
The local time density estimator,straint. Although this has proved powerful for gene identification, significant progress has also been made in uncovering gene regulatory sequences such as distant acting transcriptional enhancers. These pursuits have led to the development of a variety of valuable databases and resources that shoul作者: 思想上升 時間: 2025-3-27 01:50 作者: 左右連貫 時間: 2025-3-27 09:19
https://doi.org/10.1007/978-1-4684-0489-0abrication, as well as standardized target preparation, hybridization, and processing protocols, resulting in low technical variability and good reproducibility between experiments. This chapter describes the standard assay procedures for isolating total RNA from heart tissue, generating a biotin-la作者: Thymus 時間: 2025-3-27 11:17
Inequalities for mixing processes, that are differentially expressed as a function of time, age, genetic background or transgenic state, among other factors. SAGE is thus a powerful technique that permits a comprehensive analysis of changes in mRNA abundance. The results provide a snapshot of altered patterns of gene expression in r作者: bacteria 時間: 2025-3-27 14:46 作者: BRIDE 時間: 2025-3-27 18:11 作者: BYRE 時間: 2025-3-28 00:13
https://doi.org/10.1007/978-1-4612-1718-3 .-acting factor (antibody supershift assay), is used to identify proteins present in the DNA-protein complex. The gel-shift competition assay with an unlabeled probe containing a slightly different sequence is a powerful technique used to assess the sequence specificity and relative binding affinit作者: MOAT 時間: 2025-3-28 05:01 作者: Analogy 時間: 2025-3-28 10:19 作者: 煤渣 時間: 2025-3-28 12:54
Expression Profiling Using Affymetrix GeneChip? Probe Arraysabrication, as well as standardized target preparation, hybridization, and processing protocols, resulting in low technical variability and good reproducibility between experiments. This chapter describes the standard assay procedures for isolating total RNA from heart tissue, generating a biotin-la作者: 1分開 時間: 2025-3-28 16:13 作者: 刀鋒 時間: 2025-3-28 19:32 作者: 壓碎 時間: 2025-3-29 02:35
Measurement of Cardiac Gene Expression by Reverse Transcription Polymerase Chain Reaction (RT-PCR)r the cotransport of sodium predominantly with glutamine in exchange for hydrogen ions. The results suggested that a 20-fold increase in . expression occurs during ischemia, which reduced to baseline levels on reperfusion.作者: BOOR 時間: 2025-3-29 05:38
Characterization of ,-Regulatory Elements and Transcription Factor Binding .-acting factor (antibody supershift assay), is used to identify proteins present in the DNA-protein complex. The gel-shift competition assay with an unlabeled probe containing a slightly different sequence is a powerful technique used to assess the sequence specificity and relative binding affinit作者: Aggregate 時間: 2025-3-29 07:28
Characterization of Cardiac Gene Promoter Activity that control gene expression under different physiological stresses have been further characterized in the context of heterologous promoters to demonstrate their “stand-alone” functional properties in the absence of confounding influences from other .-elements and their cognate transcription factor作者: intercede 時間: 2025-3-29 15:17
Book 2007eir cognate cis binding elements. Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome 作者: Misnomer 時間: 2025-3-29 17:00 作者: Diastole 時間: 2025-3-29 21:43
Inequalities for mixing processes,ribes the different technical procedures that are of crucial importance to carry on sensitive and specific . hybridization experiments in heart samples. The detection of transcripts within paraformaldehyde-fixed, paraffin-embedded cardiac tissue samples is illustrated here with the detection of cardiac sphingosine-1-phosphate receptor expression.作者: cyanosis 時間: 2025-3-30 03:31 作者: Tinea-Capitis 時間: 2025-3-30 05:58
Hybridizationribes the different technical procedures that are of crucial importance to carry on sensitive and specific . hybridization experiments in heart samples. The detection of transcripts within paraformaldehyde-fixed, paraffin-embedded cardiac tissue samples is illustrated here with the detection of cardiac sphingosine-1-phosphate receptor expression.作者: 講個故事逗他 時間: 2025-3-30 09:44
Mapping Transcriptional Start Sites and , DNA Footprintingcing projects, expressed sequence tags and microarrays, combined with . analysis, such as automated sequence annotation and gene identification algorithms, now provides an alternative source of detailed information on gene structure and expression. Two approaches to the . identification of transcription factor binding sites are described.作者: 遺傳 時間: 2025-3-30 13:38
Microarray Analysis of Gene Expression in Murine Cardiac Graft Infiltrating Cellsjection by purifying subsets of infiltrating cells using GFP transgenic mice and detailed all technical experiences and issues. The purpose of this study is to assist researchers to simplify the process of analyzing large database using microarray technology.作者: mercenary 時間: 2025-3-30 20:12
