標題: Titlebook: Carbohydrate-Protein Interactions; Methods and Protocol D. Wade Abbott,Wesley F. Zandberg Book 2023Latest edition The Editor(s) (if applica [打印本頁] 作者: Washington 時間: 2025-3-21 18:20
書目名稱Carbohydrate-Protein Interactions影響因子(影響力)
作者: Brain-Waves 時間: 2025-3-21 22:56 作者: Analogy 時間: 2025-3-22 01:23
Carbohydrate-Protein Interactions978-1-0716-3151-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: SLUMP 時間: 2025-3-22 07:22
https://doi.org/10.1007/978-94-010-1724-4activity of bacterial expansin. The first assay relies on the weakening of filter paper by expansin. The second assay is based on induction of creep (long-term, irreversible extension) of plant cell wall samples.作者: Breach 時間: 2025-3-22 10:03 作者: Forage飼料 時間: 2025-3-22 16:07 作者: Forage飼料 時間: 2025-3-22 20:39 作者: Endometrium 時間: 2025-3-22 21:24
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/221540.jpg作者: Infiltrate 時間: 2025-3-23 03:10 作者: 表兩個 時間: 2025-3-23 07:08
https://doi.org/10.1007/978-3-662-21541-8ical samples or in characterization of enzyme reactions, as new reducing ends are generated when a polysaccharide substrate undergoes hydrolytic cleavage. Presented here is an application of the method in measuring the kinetics of a glycoside hydrolase reaction, including the optimization of the DNS作者: 改正 時間: 2025-3-23 11:38 作者: defenses 時間: 2025-3-23 17:41 作者: 乳白光 時間: 2025-3-23 21:48
https://doi.org/10.1007/978-94-010-1724-4activity of bacterial expansin. The first assay relies on the weakening of filter paper by expansin. The second assay is based on induction of creep (long-term, irreversible extension) of plant cell wall samples.作者: Rebate 時間: 2025-3-23 22:27 作者: AXIS 時間: 2025-3-24 03:13 作者: GROSS 時間: 2025-3-24 08:25
Victor Blasco,Alberto Daza-Garcíalear single quantum coherence (HSQC)-based techniques described in this chapter can be used quickly and effectively to screen a set of possible carbohydrate-binding partners, to quantify the dissociation constant (.) of any identified interactions, and to the map the carbohydrate-binding site on the作者: AGATE 時間: 2025-3-24 12:31 作者: Demonstrate 時間: 2025-3-24 16:40 作者: 闡明 時間: 2025-3-24 22:52 作者: atopic-rhinitis 時間: 2025-3-25 00:38
Graded Modules over Superconformal Algebraslysaccharides and the large number of CBM families displaying specific substrate recognition reflects this complexity. The accessibility of large proteins, such as antibodies, to their cell wall epitopes may be sometimes difficult due to steric hindrance problems. Due to their smaller size, CBMs are作者: aggrieve 時間: 2025-3-25 04:33
On Deformations of ,-Lie Algebrasodulates the proximity of Siglecs to other receptors that they regulate. This proximity is key to enabling signaling motifs on the cytosolic domain of Siglecs to modulate immune responses. Given the important roles that Siglecs play in helping to maintain immune homeostasis, a better understanding o作者: Indelible 時間: 2025-3-25 08:24 作者: 簡略 時間: 2025-3-25 15:00
Conclusions and Recommendations,a fingerprint of a sample with absorption peaks corresponding to the frequency of vibrations between the bonds of the atoms making up the material. Here we describe a method focused on the use of FTIR in combination with principal component analysis (PCA) to characterize the composition of the plant作者: flavonoids 時間: 2025-3-25 19:32
R. Kannan,K. R. Shanmugam,Saumitra Bhaduris, thereby allowing high-resolution separation by electrophoresis and visualization. This method can be used for carbohydrate profiling and sequencing, as well as for determining the specificity of carbohydrate-active enzymes. Here we describe and demonstrate the use of FACE to separate and visualiz作者: GLUE 時間: 2025-3-25 22:44 作者: SEEK 時間: 2025-3-26 01:48
Quantifying CBM–Carbohydrate Interactions Using Microscale Thermophoresisy constant can be obtained for a wide range of molecules within minutes based on reactions in microliters. Here we describe the application of MST in quantifying protein–carbohydrate interactions. A CBM3a and a CBM4 are titrated with insoluble substrate (cellulose nanocrystal) and soluble oligosaccharide (xylohexaose), respectively.作者: 沖突 時間: 2025-3-26 04:41 作者: 清醒 時間: 2025-3-26 11:16 作者: 代替 時間: 2025-3-26 12:58 作者: acetylcholine 時間: 2025-3-26 19:06
Carbohydrate Depolymerization by Intricate Cellulosomal Systems in turn fostered our capacity to enhance the catalytic potential of artificial cellulolytic complexes. In this chapter, methods to produce and employ such intricate cellulosomal complexes are reported.作者: 癡呆 時間: 2025-3-27 00:38
Toka Diagana,Fran?ois Ramarosonity, whereas C4 oxidation leads to products that are inherently labile at high and low pH and that exist in a keto-gemdiol equilibrium that is strongly shifted towards the gemdiol in aqueous solutions. Partial degradation of C4-oxidized products leads to the formation of native products, which could作者: 整潔 時間: 2025-3-27 01:45
Non-Archimedean Operator Theory in turn fostered our capacity to enhance the catalytic potential of artificial cellulolytic complexes. In this chapter, methods to produce and employ such intricate cellulosomal complexes are reported.作者: hegemony 時間: 2025-3-27 06:08
1064-3745 ation advice from the expertsThis second edition provides new and updated tools for studying protein-carbohydrate interactions ranging from traditional biochemical methods to state-of-the-art techniques. This book focuses on four different research themes detailing methods for screening and quantify作者: 食品室 時間: 2025-3-27 10:02 作者: 啜泣 時間: 2025-3-27 15:45 作者: stratum-corneum 時間: 2025-3-27 18:56
Growth Functions of Jordan Algebrase surface binding sites of proteins, mostly enzymes, have also been investigated by this method. Here we describe a protocol for identifying binding interactions between enzyme catalytic modules and a variety of carbohydrate ligands.作者: 拱墻 時間: 2025-3-28 00:19
Non-Associative Algebras and Related Topicsthalpic and entropic contributions to this interaction, without the use of labeled proteins or substrates. Here we describe a standard multiple-injection titration experiment for measuring the binding energetics between a carbohydrate-binding protein and an oligosaccharide.作者: Decibel 時間: 2025-3-28 04:10 作者: troponins 時間: 2025-3-28 09:27
Graded Modules over Superconformal Algebraseins, such as antibodies, to their cell wall epitopes may be sometimes difficult due to steric hindrance problems. Due to their smaller size, CBMs are interesting alternative probes. The aim of this chapter is to describe the use of CBM as probes to explore complex polysaccharide topochemistry . and to quantify enzymatic deconstruction.作者: 揮舞 時間: 2025-3-28 10:54 作者: Homocystinuria 時間: 2025-3-28 15:21 作者: 膠狀 時間: 2025-3-28 20:49
Measuring Enzyme Kinetics of Glycoside Hydrolases Using the 3,5-Dinitrosalicylic Acid Assayage. Presented here is an application of the method in measuring the kinetics of a glycoside hydrolase reaction, including the optimization of the DNSA reagent, and the production of a standard curve of absorbance versus sugar concentration.作者: 袋鼠 時間: 2025-3-29 00:39 作者: 和藹 時間: 2025-3-29 05:51
Isothermal Titration Calorimetry for Quantification of Protein–Carbohydrate Interactionsthalpic and entropic contributions to this interaction, without the use of labeled proteins or substrates. Here we describe a standard multiple-injection titration experiment for measuring the binding energetics between a carbohydrate-binding protein and an oligosaccharide.作者: 惡名聲 時間: 2025-3-29 09:08
Surface Plasmon Resonance Analysis for Quantifying Protein–Carbohydrate Interactionsareful experimental design to avoid. Here we give an overview of each step in the SPR analysis from immobilization to data analysis, providing key points of consideration that will allow practitioners to achieve reliable and reproducible results.作者: Atheroma 時間: 2025-3-29 11:34 作者: Antigen 時間: 2025-3-29 16:24 作者: Systemic 時間: 2025-3-29 23:01
Book 2023Latest editione-of-the-art techniques. This book focuses on four different research themes detailing methods for screening and quantifying CAZyme activity, investigating the interactions between proteins, carbohydrate ligands, methods for the visualization of carbohydrates, protein-carbohydrate complexes, structu作者: BUOY 時間: 2025-3-30 02:51 作者: 公共汽車 時間: 2025-3-30 05:23
Extraction and Verification of Mouse and Human Mucins from Tissue and Fecal Materialds are unable to effectively separate these glycoproteins for analysis. We describe the procedure for making composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels, which allows for accurate verification and band separation of extracted mucins.作者: padding 時間: 2025-3-30 10:59 作者: 禍害隱伏 時間: 2025-3-30 15:27
Victor Blasco,Alberto Daza-Garcíalactosamine (GalNAc), in which we calculate the apparent dissociation of the interaction and map the GalNAc binding site onto the structure of .CBM32. This approach can be applied to other CBM– and protein–ligand systems.作者: 脾氣暴躁的人 時間: 2025-3-30 17:26
On Deformations of ,-Lie Algebrasle versions of the recombinant Siglecs in conjunction with flow cytometry. Flow cytometry has many advantages in enabling the relative levels of Siglec ligands between cell types to be rapidly quantified. Here, a step-by-step protocol is described on how to detect Siglec ligands most sensitively and accurately on cells by flow cytometry.
