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標(biāo)題: Titlebook: Capillary Electrophoresis: Instrumentation and Operation; Wim Kok Book 2000 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH, Braunschweig/Wi [打印本頁]

作者: analgesic    時(shí)間: 2025-3-21 17:45
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作者: Kidnap    時(shí)間: 2025-3-21 23:12
1615-2255 the almost 20 years since its first appearance, Capil- Writing a monograph like this is a lot of work, even more lary Electrophoresis (CE) has become a standard analy- than I anticipated. Luckily, I could fall back on the tical separation technique in many laboratories. CE is theoretical and practic
作者: LATE    時(shí)間: 2025-3-22 03:02

作者: 巧辦法    時(shí)間: 2025-3-22 06:39
https://doi.org/10.1007/978-1-4614-7726-6tion of two adjacent peaks by their resolution (. .). Occasionally the similarity between chromatography and CE is overestimated, when an efficiency is expressed as the number of plates per meter. As will be shown below, plate numbers in CE do not increase proportionally with the capillary length.
作者: 遺產(chǎn)    時(shí)間: 2025-3-22 12:15

作者: Militia    時(shí)間: 2025-3-22 13:39
A. Tasidou,P. S. Efraimidis,V. Katose(s). In other respects there are cardinal differences between the two. Amperometric detection relies on the chemical characteristics of specific analytes, while with conductivity detection a bulk property of the separation medium is monitored. The first technique is therefore selective, the second inherently non-selective.
作者: Militia    時(shí)間: 2025-3-22 18:34

作者: Inflammation    時(shí)間: 2025-3-23 00:02
Efficiency and Resolution,tion of two adjacent peaks by their resolution (. .). Occasionally the similarity between chromatography and CE is overestimated, when an efficiency is expressed as the number of plates per meter. As will be shown below, plate numbers in CE do not increase proportionally with the capillary length.
作者: SLAG    時(shí)間: 2025-3-23 04:17
Voltages and Currents,rical insulation of the various parts of the CE set-up becomes problematic, with spontaneous sparking and a risk of serious damage of sensitive electronic components as a result. A high precision and low drift of the applied voltage is of course important for the reproducibility of the migration times of analytes.
作者: aesthetic    時(shí)間: 2025-3-23 06:26

作者: 世俗    時(shí)間: 2025-3-23 10:57
Athanasios Karantjias,Nineta Polemi Figure 13.1. A racemic mixture of terbutaline was separated by CE under overloaded conditions (Figure 13.1a). The reinjection of one of the collected fractions shows the enantiomeric purity of the product (Figure 13.1b).
作者: 巡回    時(shí)間: 2025-3-23 15:35

作者: 乞丐    時(shí)間: 2025-3-23 18:32

作者: Indebted    時(shí)間: 2025-3-24 01:01
Genomic Landscape of Cancer Metastasis,first of Pyrex glass and later of fused silica. The other crucial factor for the development of CE was the availability of detectors suitable for the small volume scale employed. Jorgenson and Lukacs used oncolumn fluorescence detection.
作者: Gullible    時(shí)間: 2025-3-24 03:33
Kumari Namrata,Akshit Samadhiya,Papia Raying the separation, and to make these conditions independent of the sample composition. Only under such conditions one can expect to be able to identify a sample component by its migration time in CZE.
作者: Explosive    時(shí)間: 2025-3-24 07:38

作者: Ovulation    時(shí)間: 2025-3-24 13:43
Thermal Management,first of Pyrex glass and later of fused silica. The other crucial factor for the development of CE was the availability of detectors suitable for the small volume scale employed. Jorgenson and Lukacs used oncolumn fluorescence detection.
作者: 不能根除    時(shí)間: 2025-3-24 16:01

作者: Expertise    時(shí)間: 2025-3-24 22:24

作者: ethnology    時(shí)間: 2025-3-25 02:43
Jian Chen,Lopa Mishra,Xiaoping Suhe electroosmotic mobility is in principle governed by the chemical condition of the inner surface of the capillary and by the composition of the background electrolyte (BGE) used for the separation. Still, even with careful control of these parameters, large variations in the EOF are found in practice.
作者: lethargy    時(shí)間: 2025-3-25 03:40
Instrumental Developments,e sold (by PE Biosystems) as Genetic Analyzers. Of course, there is nothing against using these instruments in other type of applications. Still, a number of suppliers offer general-purpose CE instruments; at present (end of 1999) in Europe these companies are Agilent (formerly known as the Hewlett
作者: 裂隙    時(shí)間: 2025-3-25 09:26
1615-2255 ical techniques. This seemed about it later. Together we formed maybe not the most a good moment to summarize the instrumental possibi- famous but certainly the best-spirited CE research group lities of the technique, its accomplishments and weak in the world. T978-3-322-83135-4978-3-322-83133-0Series ISSN 1615-2255
作者: Slit-Lamp    時(shí)間: 2025-3-25 13:29
Capillary Electrophoresis: Instrumentation and Operation
作者: 褪色    時(shí)間: 2025-3-25 18:46
Book 2000son or two the establishment of analytical techniques. This seemed about it later. Together we formed maybe not the most a good moment to summarize the instrumental possibi- famous but certainly the best-spirited CE research group lities of the technique, its accomplishments and weak in the world. T
作者: Chromatic    時(shí)間: 2025-3-25 20:39

作者: LUCY    時(shí)間: 2025-3-26 02:30
Emergence of Next-Generation Sequencing,Electrophoresis is the migration of charged particles in a solution under the influence of an electric field. Different particles with different charges and/or sizes migrate with different velocities: this is the basic principle of all electrophoretic separation methods.
作者: Ordnance    時(shí)間: 2025-3-26 05:56

作者: 無能力之人    時(shí)間: 2025-3-26 11:10

作者: 兒童    時(shí)間: 2025-3-26 13:21
Sample Introduction,Basically there are two different methods to introduce the sample into the first small part of the capillary: hydrodynamic (driven by a pressure difference over the capillary) and electrokinetic. The differences between the two methods will be discussed.
作者: Medicaid    時(shí)間: 2025-3-26 19:55
Wim Kokaktuelle, wissenschaftlich hochwertige Informationen zu ausgew?hlten Themen der Kapillarelektrophorese
作者: INCH    時(shí)間: 2025-3-26 23:42
CHROMATOGRAPHIA CE-Serieshttp://image.papertrans.cn/c/image/221399.jpg
作者: 女上癮    時(shí)間: 2025-3-27 04:41

作者: 意外的成功    時(shí)間: 2025-3-27 08:22

作者: ADORN    時(shí)間: 2025-3-27 13:06
https://doi.org/10.1007/978-1-4614-7645-0urce is therefore a prerequisite for CE. Commercial instruments have sources with an upper limit of usually 30 or 35 kV With higher voltages the electrical insulation of the various parts of the CE set-up becomes problematic, with spontaneous sparking and a risk of serious damage of sensitive electr
作者: 牽索    時(shí)間: 2025-3-27 15:11
Genomic Landscape of Cancer Metastasis,ral research groups [2, 3, 4] published on “free zone electrophoresis” in narrow tubes, which is essentially the same as CE. The break-through in 1981 was achieved by the improvement of the thermal management of the system, simply by decreasing the diameter of the separation channel. This scaling-do
作者: SPER    時(shí)間: 2025-3-27 21:28
Jian Chen,Lopa Mishra,Xiaoping Sumobilities that can be handled in a single run as well as the run time. Moreover, variations in the EOF, between different capillaries and between different runs on the same capillary, are the main source of the irrepeatability and irreproducibility of the elution times so often encountered in CE. T
作者: Chromatic    時(shí)間: 2025-3-28 00:40

作者: etidronate    時(shí)間: 2025-3-28 02:14
Next Generation Smart Nano-Bio-Devicesments with a single-and/or multi-wavelength absorption detector, while other detector options, such as a lamp- or laser-induced fluorescence detector or a conductivity detector, are commercially available from only a few manufacturers. Probably over 95% of the routine applications of CE is carried o
作者: 狂熱語言    時(shí)間: 2025-3-28 06:48
Vassilis Meneklis,Christos Douligeristion with fluorescence are often two to three orders of magnitude lower than with UV absorbance detection. Since in CE with its small dimensions it is to be expected that detection sensitivity may be problematic for many applications, especially for trace analysis, it seems obvious to develop or mod
作者: 綠州    時(shí)間: 2025-3-28 13:15
Triantafillou Vasilis,Kalogeras Dimitristes that are otherwise difficult to measure with spectroscopic techniques [1,2,3,4]. Indirect detection can also be applied in various variants of liquid chromatography; there, however, it has never become popular. In CE on the other hand, indirect detection has often been applied, if only for the s
作者: seroma    時(shí)間: 2025-3-28 16:27
A. Tasidou,P. S. Efraimidis,V. Katosn. Both modes are invasive techniques, i.e., they both require a direct contact between the separation medium and the sensing element(s), the electrode(s). In other respects there are cardinal differences between the two. Amperometric detection relies on the chemical characteristics of specific anal
作者: 遭受    時(shí)間: 2025-3-28 18:44

作者: 著名    時(shí)間: 2025-3-29 02:45

作者: beta-carotene    時(shí)間: 2025-3-29 05:12

作者: LEERY    時(shí)間: 2025-3-29 10:04
978-3-322-83135-4Friedr. Vieweg & Sohn Verlagsgesellschaft mbH, Braunschweig/Wiesbaden 2000
作者: insurrection    時(shí)間: 2025-3-29 11:46

作者: Immobilize    時(shí)間: 2025-3-29 16:08

作者: 陪審團(tuán)每個(gè)人    時(shí)間: 2025-3-29 21:08
Capillary Electrophoresis: Instrumentation and Operation978-3-322-83133-0Series ISSN 1615-2255
作者: Rheumatologist    時(shí)間: 2025-3-30 02:59

作者: 用手捏    時(shí)間: 2025-3-30 05:02
Vassilis Meneklis,Christos Douligeristion with fluorescence are often two to three orders of magnitude lower than with UV absorbance detection. Since in CE with its small dimensions it is to be expected that detection sensitivity may be problematic for many applications, especially for trace analysis, it seems obvious to develop or modify a fluorescence detector for use in CE.
作者: PALMY    時(shí)間: 2025-3-30 10:27
The Short History of CE,tially the same as we know it today, can be found in 1981 with the publication of an article by Jorgenson and Lukacs, working at the University of North Carolina (USA), in Analytical Chemistry [1]. They showed the separation of dansyl and fluorescamine derivatized amino acids, peptides and amines in
作者: subacute    時(shí)間: 2025-3-30 13:27





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