標題: Titlebook: Cancer Vaccines; Methods and Protocol Michael J.P. Lawman,Patricia D. Lawman Book 2014 Springer Science+Business Media New York 2014 Cancer [打印本頁] 作者: MIFF 時間: 2025-3-21 16:33
書目名稱Cancer Vaccines影響因子(影響力)
書目名稱Cancer Vaccines影響因子(影響力)學科排名
書目名稱Cancer Vaccines網(wǎng)絡公開度
書目名稱Cancer Vaccines網(wǎng)絡公開度學科排名
書目名稱Cancer Vaccines被引頻次
書目名稱Cancer Vaccines被引頻次學科排名
書目名稱Cancer Vaccines年度引用
書目名稱Cancer Vaccines年度引用學科排名
書目名稱Cancer Vaccines讀者反饋
書目名稱Cancer Vaccines讀者反饋學科排名
作者: Forage飼料 時間: 2025-3-21 22:54
Generation of Multiple Peptide Cocktail-Pulsed Dendritic Cells as a Cancer Vaccineer immunotherapy is the generation of antigen-specific cytotoxic T lymphocyte (CTL) response. Tumor-associated antigens (TAA) and DC play pivotal roles in this process. DCs are well known to be the most potent antigen-presenting cells and have the most powerful antigen-presenting capacity. DCs pulse作者: Inculcate 時間: 2025-3-22 03:32 作者: Spongy-Bone 時間: 2025-3-22 05:21 作者: 有幫助 時間: 2025-3-22 11:04
Ex Vivo Loading of Autologous Dendritic Cells with Tumor Antigensr antigens is a strategy to arm DC against tumor without human leukocyte antigen (HLA) restriction. Furthermore, this approach allows the presentation of a full antigen range to the immune system. We describe the methods to obtain whole antigens from autologous tumor tissues in order to load DC gene作者: LANCE 時間: 2025-3-22 15:03 作者: LANCE 時間: 2025-3-22 19:32 作者: Iatrogenic 時間: 2025-3-22 23:26 作者: Counteract 時間: 2025-3-23 05:16 作者: 清唱劇 時間: 2025-3-23 08:22 作者: ferment 時間: 2025-3-23 09:56 作者: 昏睡中 時間: 2025-3-23 15:32
Fast Monocyte-Derived Dendritic Cell-Based Immunotherapyn vitro culture (fast-DC). Mature fast-DC are as effective as mature standard-DC (generated in 7–10 days of in vitro culture) in priming and propagation of antigen-specific T-cell responses. The use of fast-DC not only reduces labor and supply cost, as well as workload and time, but also increases t作者: Expediency 時間: 2025-3-23 21:20
Intratumoral Injection of BCG-CWS-Pretreated Dendritic Cells Following Tumor Cryoablation induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wa作者: CURL 時間: 2025-3-24 02:13
Exploiting the CD1d-iNKT Cell Axis for Potentiation of DC-Based Cancer Vaccinesr cells by pro-inflammatory cytokines. Evidence is accumulating that the CD1d-iNKT cell axis can be effectively used to potentiate DC-based cancer vaccines. Here, we provide a detailed methodology for the generation of (CD1d-expressing) monocyte-derived DC (moDC) and their subsequent loading with th作者: 氣候 時間: 2025-3-24 03:15 作者: TAG 時間: 2025-3-24 10:03
Genetic Modification of Mouse Effector and Helper T Lymphocytes Expressing a Chimeric Antigen Receptmunotherapy strategies. However, limitations in current protocols for generating highly pure and sufficient numbers of enriched effector and helper CAR. T cell subsets remain problematic. Here, we describe a new retroviral transduction protocol for successfully generating transduced CD8. and CD4. T 作者: Mast-Cell 時間: 2025-3-24 11:51 作者: micturition 時間: 2025-3-24 18:34
1064-3745 ail essential for reproducible results.Contains key notes an.Cancer Vaccines: Methods and Protocols. explores the manipulation and modification of immune cells, the manipulation and modification of tumor cells as well as the manipulation of immune/tumor interactions and various delivery mechanisms, 作者: 接合 時間: 2025-3-24 22:37
https://doi.org/10.1007/978-981-10-3872-3 of a full antigen range to the immune system. We describe the methods to obtain whole antigens from autologous tumor tissues in order to load DC generated ex vivo from patients with gastrointestinal cancer.作者: 粉筆 時間: 2025-3-25 02:59
https://doi.org/10.1007/978-981-10-3872-3r designing an effective tumor vaccine protocol. Here, we describe the stimulation of purified splenic- or bone marrow-derived DC with recombinant interleukin-15 (IL-15) in the presence of intact soluble antigen from metastatic lymphoma tumor cells in an experimental animal model.作者: Commemorate 時間: 2025-3-25 06:44
Systems of Dynamic Simultaneous Equationsasmic degradation pathway and can be presented by DC through class I major histocompatibility complex (MHC)-I, thus inducing specific T cell cytotoxic responses. In this chapter, we present a protocol to transfect murine dendritic cells with tumor mRNA by means of electroporation.作者: Flinch 時間: 2025-3-25 08:53
https://doi.org/10.1007/978-0-387-72596-3cines. Here, we provide a detailed methodology for the generation of (CD1d-expressing) monocyte-derived DC (moDC) and their subsequent loading with the iNKT cell agonist α-galactosylceramide (α-GalCer) or their direct ligation by agonistic anti-CD1d monoclonal antibodies.作者: frivolous 時間: 2025-3-25 14:38
Paola Brighi,Giuseppe TorluccioR. T cell subsets remain problematic. Here, we describe a new retroviral transduction protocol for successfully generating transduced CD8. and CD4. T lymphocytes for in vitro and in vivo characterization.作者: DUST 時間: 2025-3-25 16:38
Ex Vivo Loading of Autologous Dendritic Cells with Tumor Antigens of a full antigen range to the immune system. We describe the methods to obtain whole antigens from autologous tumor tissues in order to load DC generated ex vivo from patients with gastrointestinal cancer.作者: ITCH 時間: 2025-3-25 22:12 作者: 史前 時間: 2025-3-26 04:08
Antigen-Specific mRNA Transfection of Autologous Dendritic Cellsasmic degradation pathway and can be presented by DC through class I major histocompatibility complex (MHC)-I, thus inducing specific T cell cytotoxic responses. In this chapter, we present a protocol to transfect murine dendritic cells with tumor mRNA by means of electroporation.作者: 截斷 時間: 2025-3-26 05:03 作者: 思想 時間: 2025-3-26 08:50 作者: Motilin 時間: 2025-3-26 15:40
Apes, Humans, and Molecular Clocksimulatory molecules, including CD70, caTLR4, and CD40L (TriMix-DC), leads to fully potent antigen-presenting DC able to generate a broad immune response..Here we describe the in vitro transcription of the mRNA and the subsequent generation and electroporation of autologous DC used for the treatment of melanoma patients.作者: 玩忽職守 時間: 2025-3-26 20:44 作者: jealousy 時間: 2025-3-26 21:15
VAR Processes with Parameter Constraintsony-stimulating factor (GM-CSF), anti-DEC-205 monoclonal antibodies, flagellin, and heat shock proteins (HSP), function as promising intermolecular adjuvants. Herein, we describe in vitro assays on human DC pulsed with HSP fusion proteins, which might be useful in preclinical studies for the screening and assessment of candidate cancer vaccines.作者: Gleason-score 時間: 2025-3-27 02:21 作者: 燒瓶 時間: 2025-3-27 05:27
Examining Diversity in Islamic Schools,ll fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.作者: 上下倒置 時間: 2025-3-27 11:54 作者: 思想上升 時間: 2025-3-27 17:34
Book 2014as well as the manipulation of immune/tumor interactions and various delivery mechanisms, with the overall end goal of evoking a tumor-specific response and overcoming the immuno-evasive mechanisms employed by the tumor cells.?This detailed volume also covers the subject of cancer vaccines in a more作者: Hyperlipidemia 時間: 2025-3-27 19:55
Single-Step Antigen Loading and Maturation of Dendritic Cells Through mRNA Electroporation of a Tumoimulatory molecules, including CD70, caTLR4, and CD40L (TriMix-DC), leads to fully potent antigen-presenting DC able to generate a broad immune response..Here we describe the in vitro transcription of the mRNA and the subsequent generation and electroporation of autologous DC used for the treatment of melanoma patients.作者: 持久 時間: 2025-3-28 00:35
Pulsing Dendritic Cells with Whole Tumor Cell Lysatesations must be taken into account to obtain a lysate with appropriate biological activity, such as cell line harvest and the method to lyse the cells. In this chapter, we describe the steps to obtain whole tumor cell lysates from human tumor cell lines by repetitive freeze–thaw cycles in sufficient amount and quality to pulse DC.作者: 圖表證明 時間: 2025-3-28 03:07 作者: 我吃花盤旋 時間: 2025-3-28 08:20 作者: 發(fā)酵劑 時間: 2025-3-28 13:23
Intratumoral Injection of BCG-CWS-Pretreated Dendritic Cells Following Tumor Cryoablationll fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.作者: 不整齊 時間: 2025-3-28 15:32
Modification of T Lymphocytes to Express Tumor Antigensuch as interleukin-7 (IL-7) and interleukin-12 (IL-12). Here, we describe the technique of preparing activated human TAPC pulsed with TAA peptides for the induction of tumor antigen-specific T cell immunity in vitro.作者: Hormones 時間: 2025-3-28 19:04
Estimation of Vector Error Correction Modelsis chapter, we outline a functionally closed, good manufacturing protocol for the differentiation of monocytes into DCs and transduction by Ad vectors. Basic functional and phenotypic release assays are provided, as well as contrasting research approaches for Ad-transduced DC-based vaccines.作者: sleep-spindles 時間: 2025-3-29 01:09 作者: Lumbar-Stenosis 時間: 2025-3-29 05:16 作者: indicate 時間: 2025-3-29 07:25
Fast Monocyte-Derived Dendritic Cell-Based Immunotherapyn sources, and transduction with adenoviral vector of Mo-derived mature fast-DC as well as using of fast-DC for priming and propagation of antigen-specific cytotoxic T-cell effectors will be described here.作者: 思想上升 時間: 2025-3-29 11:58
Michael J.P. Lawman,Patricia D. LawmanIncludes cutting-edge methods and protocols to support the creation of future safe and efficacious cancer vaccines.Provides step-by-step detail essential for reproducible results.Contains key notes an作者: NIB 時間: 2025-3-29 18:23 作者: 某人 時間: 2025-3-29 20:39 作者: obnoxious 時間: 2025-3-30 00:08
Apes, Humans, and Molecular Clocksyte fraction of the peripheral blood. They are the professional antigen-presenting cells, and electroporation of mRNA-encoding tumor antigens is a very efficient and a relatively simple way to load the DC with antigen. The co-electroporation of a tumor antigen of choice and the combination of 3 cost作者: 后退 時間: 2025-3-30 05:12
M. Goodman,M. L. Baba,L. L. Dargaer immunotherapy is the generation of antigen-specific cytotoxic T lymphocyte (CTL) response. Tumor-associated antigens (TAA) and DC play pivotal roles in this process. DCs are well known to be the most potent antigen-presenting cells and have the most powerful antigen-presenting capacity. DCs pulse作者: Receive 時間: 2025-3-30 09:52
https://doi.org/10.1007/978-981-10-3872-3roaches have been used to obtain and load tumor antigens in DC. One such technique is to use whole tumor cell lysate from one or more tumor cell lines of the tumor type to be treated. The advantage of applying this method is that it provides a large spectrum of tumor antigens. However, some consider作者: 知識 時間: 2025-3-30 14:10
https://doi.org/10.1007/978-981-10-3872-3ls. Therefore, DC are being extensively evaluated as vehicles for antigen delivery in immunotherapies for the treatment of patients with cancer. Many techniques have been used to load DC with tumor-associated antigens (TAA), including pulsing with synthetic peptides that represent T cell epitopes. T作者: 蒸發(fā) 時間: 2025-3-30 17:44
https://doi.org/10.1007/978-981-10-3872-3r antigens is a strategy to arm DC against tumor without human leukocyte antigen (HLA) restriction. Furthermore, this approach allows the presentation of a full antigen range to the immune system. We describe the methods to obtain whole antigens from autologous tumor tissues in order to load DC gene作者: 尖 時間: 2025-3-30 23:35
https://doi.org/10.1007/978-981-10-3872-3essential components like genes encoding cytokines, chemokines, and other molecules or stimulation with recombinant cytokines is a potential method for designing an effective tumor vaccine protocol. Here, we describe the stimulation of purified splenic- or bone marrow-derived DC with recombinant int作者: Vldl379 時間: 2025-3-31 02:18
VAR Processes with Parameter Constraintscapable of stimulating or targeting dendritic cells (DC), the most important antigen-presenting cells for inducing CD8. cytotoxic T lymphocytes (CTL) which can efficiently kill tumor cells expressing the tumor antigen. The DC-stimulating or DC-targeting proteins, including granulocyte/macrophage col作者: FLINT 時間: 2025-3-31 08:02 作者: Relinquish 時間: 2025-3-31 11:09 作者: CLAN 時間: 2025-3-31 16:58
Estimation of Vector Error Correction Modelsthe unique capacity to stimulate an antigen-specific T-cell responses by na?ve T cells. Adenoviruses (Ad) have high transduction efficiency for many cell types including cells of hematopoietic origin independent of their mitotic status, and replication-defective Ad have demonstrated a safety profile作者: Sarcoma 時間: 2025-3-31 19:40 作者: Heterodoxy 時間: 2025-3-31 22:29 作者: 健談 時間: 2025-4-1 02:47
Examining Diversity in Islamic Schools, induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wa作者: 行乞 時間: 2025-4-1 06:25 作者: 寬容 時間: 2025-4-1 10:34 作者: HACK 時間: 2025-4-1 14:20
Paola Brighi,Giuseppe Torlucciomunotherapy strategies. However, limitations in current protocols for generating highly pure and sufficient numbers of enriched effector and helper CAR. T cell subsets remain problematic. Here, we describe a new retroviral transduction protocol for successfully generating transduced CD8. and CD4. T 作者: 鴿子 時間: 2025-4-1 20:27 作者: 進入 時間: 2025-4-1 23:41
https://doi.org/10.1007/978-1-4939-0345-0Cancer immunotherapies; Delivery mechanisms; Dendritic cells; Immune cell manipulation; Immune system; Im
