標(biāo)題: Titlebook: Cancer Genomics and Proteomics; Methods and Protocol Paul B. Fisher Book 2007 Humana Press 2007 BAC.Carcinom.Chromosom.DNA.Microarray.Trans [打印本頁] 作者: 不幸的你 時(shí)間: 2025-3-21 18:50
書目名稱Cancer Genomics and Proteomics影響因子(影響力)
書目名稱Cancer Genomics and Proteomics影響因子(影響力)學(xué)科排名
書目名稱Cancer Genomics and Proteomics網(wǎng)絡(luò)公開度
書目名稱Cancer Genomics and Proteomics網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cancer Genomics and Proteomics被引頻次
書目名稱Cancer Genomics and Proteomics被引頻次學(xué)科排名
書目名稱Cancer Genomics and Proteomics年度引用
書目名稱Cancer Genomics and Proteomics年度引用學(xué)科排名
書目名稱Cancer Genomics and Proteomics讀者反饋
書目名稱Cancer Genomics and Proteomics讀者反饋學(xué)科排名
作者: 加入 時(shí)間: 2025-3-21 23:17
Cloning Differentially Expressed Genes Using Rapid Subtraction Hybridization (RaSH), differentially expressed genes represent critical elements in this process. Many techniques have been developed to facilitate achieving these objectives. Although effective in many situations, most currently described approaches are not trouble-free and have limitations, including complexity of per作者: 入會(huì) 時(shí)間: 2025-3-22 04:02
The Application of Differential Display as a Gene Profiling Tool,fications that have been described over the last several years. A highly reproducible, semihigh-throughput differential display protocol used in our laboratories is described along with an example of its successful application using pancreatic cancer cells. In addition to the work performed in our l作者: 透明 時(shí)間: 2025-3-22 06:00 作者: 劇本 時(shí)間: 2025-3-22 10:35
Gene Expression Profile Analysis of Tumors,les for all known genes in the human genome. The genome-wide analysis of the gene expression patterns of neoplastic and normal cells provides insights into: (1) the identification of previously unknown tumor subtypes; (2) the normal cellular counterparts of tumor cells; (3) the identification of cel作者: notion 時(shí)間: 2025-3-22 13:30 作者: notion 時(shí)間: 2025-3-22 20:35 作者: Adenoma 時(shí)間: 2025-3-22 22:06
Complete Open Reading Frame (C-ORF) Technique,CR. However, these protocols are not infallible and in specific instances they have proven unsuccessful, emphasizing a need for further refinement. A novel method, the complete open reading frame (C-ORF) technique, is presently described, which has proven successful in cases, where standard rapid am作者: aggrieve 時(shí)間: 2025-3-23 05:27
Chromatin Immunoprecipitation Assays,es, their downstream targets, or owing to increased proliferation, and altered apoptotic potential. Various microarray based techniques have been widely utilized to study the differential expression of genes in cancer in recent years. Along with this, attempts have been made to study the transcripti作者: Charade 時(shí)間: 2025-3-23 05:42
Manipulating Genes and Gene Copy Number by Bacterial Artificial Chromosome Transfection,nd other large genomic loci. Protocols and parameters that influence the efficient transfection of these large DNA molecules into cells in culture were described here. By carefully optimizing the conditions for the formation of compact transfection complexes, BACs can be introduced into a variety of作者: 恩惠 時(shí)間: 2025-3-23 12:54
Molecular Cytogenetic Applications in Analysis of the Cancer Genome,ation of complex and cryptic chromosomal changes is beyond the resolution of conventional banding methods. The fluorescence microscopy afforded by imaging technologies, developed recently, facilitates a precise identification of these chromosome alterations in cancer. The three most commonly utilize作者: neologism 時(shí)間: 2025-3-23 17:40 作者: 移植 時(shí)間: 2025-3-23 20:03
The Use of Phage Display Peptide Libraries for Basic and Translational Research,at proteins. The gene product is encoded by a plasmid contained within the virus, which can be recovered and sequenced, linking the genetic information to the function of the protein. Phage display offers a powerful tool for the identification of short peptides or single chain antibodies that can bi作者: PHONE 時(shí)間: 2025-3-24 02:08 作者: 改良 時(shí)間: 2025-3-24 03:48 作者: Entropion 時(shí)間: 2025-3-24 09:51
Surface-Epitope Masking (SEM),ntibodies (MAbs) reacting with both known and unknown molecules expressed on the cell surface. The tenet underlying SEM involves blocking (masking) of shared antigens between two target populations, a “driver” and a “tester,” and using appropriately modified surface-masked “tester” cells to generate作者: 公共汽車 時(shí)間: 2025-3-24 11:53
Approaches for Monitoring Signal Transduction Changes in Normal and Cancer Cells,ism examined the activities of several highly specific protein and carbohydrate kinases in cell lysates or isolated cell fractions. As more protein kinases were discovered in the 1980s and 1990s, coupled with the development of immunoprecipitating antibodies, in vitro kinase assays of isolated kinas作者: 乏味 時(shí)間: 2025-3-24 18:15
PKR in Innate Immunity, Cancer, and Viral Oncolysis,ed immune program. Key components of innate immunity are the interferons (IFNs), a family of related cytokines with potent antimicrobial and immuno-modulatory activities. The IFNs exert their effects through the induction of numerous genes, one of which is the double-stranded RNA-dependent protein k作者: freight 時(shí)間: 2025-3-24 22:58
Analysis of Transformation and Tumorigenicity Using Mouse Embryonic Fibroblast Cells,ellular senescence that imposes a finite number of divisions in culture. Primary mouse embryonic fibroblast (MEF) cells have a limited growth capacity and on prolonged passaging spontaneously immortalize at a low frequency. In contrast to transformation of primary MEF cells that requires the presenc作者: Malaise 時(shí)間: 2025-3-25 01:35 作者: Directed 時(shí)間: 2025-3-25 06:35 作者: GROUP 時(shí)間: 2025-3-25 10:59
Financial and Monetary Policy Studiesfications that have been described over the last several years. A highly reproducible, semihigh-throughput differential display protocol used in our laboratories is described along with an example of its successful application using pancreatic cancer cells. In addition to the work performed in our l作者: 一美元 時(shí)間: 2025-3-25 13:02 作者: insurgent 時(shí)間: 2025-3-25 19:25 作者: TSH582 時(shí)間: 2025-3-25 22:34 作者: Abduct 時(shí)間: 2025-3-26 01:42
Arto K?li?,Mari Honkanen,Jukka Lahdensivuue-based microarray approaches have made possible another scientific approach. This is one that interrogates comprehensively the complex profile of mRNA or protein expression present in normal, preneoplastic, or malignant cells and tissues. This in turn can uncover critical targets for cancer pharma作者: 玩笑 時(shí)間: 2025-3-26 05:00 作者: Circumscribe 時(shí)間: 2025-3-26 10:16
https://doi.org/10.1007/978-981-32-9588-9es, their downstream targets, or owing to increased proliferation, and altered apoptotic potential. Various microarray based techniques have been widely utilized to study the differential expression of genes in cancer in recent years. Along with this, attempts have been made to study the transcripti作者: Mingle 時(shí)間: 2025-3-26 14:27 作者: 障礙 時(shí)間: 2025-3-26 20:11
Renu Jatana,Mehjabeen Barodawalaation of complex and cryptic chromosomal changes is beyond the resolution of conventional banding methods. The fluorescence microscopy afforded by imaging technologies, developed recently, facilitates a precise identification of these chromosome alterations in cancer. The three most commonly utilize作者: 克制 時(shí)間: 2025-3-26 21:18 作者: mortuary 時(shí)間: 2025-3-27 01:49
Diana Kopeva,Iliya Kereziev,Nikolay Sterevat proteins. The gene product is encoded by a plasmid contained within the virus, which can be recovered and sequenced, linking the genetic information to the function of the protein. Phage display offers a powerful tool for the identification of short peptides or single chain antibodies that can bi作者: 珊瑚 時(shí)間: 2025-3-27 08:38
Sayed Mohamed Saeed,Adel M. Sareadely used to identify and explore protein-protein interactions. By using various two-hybrid systems, numerous protein interactions that regulate apoptosis signaling have been discovered that reveal unexpected functions of cancer-relevant proteins. Methods for performing two-hybrid experiments using 作者: 有雜色 時(shí)間: 2025-3-27 11:25 作者: 名詞 時(shí)間: 2025-3-27 15:24
F. Pervin Bilir,Seyhan Bilir Gülerntibodies (MAbs) reacting with both known and unknown molecules expressed on the cell surface. The tenet underlying SEM involves blocking (masking) of shared antigens between two target populations, a “driver” and a “tester,” and using appropriately modified surface-masked “tester” cells to generate作者: 畏縮 時(shí)間: 2025-3-27 20:08 作者: Acupressure 時(shí)間: 2025-3-28 00:46 作者: Commemorate 時(shí)間: 2025-3-28 04:56 作者: Uncultured 時(shí)間: 2025-3-28 08:45 作者: Evacuate 時(shí)間: 2025-3-28 14:03
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/221118.jpg作者: Brain-Waves 時(shí)間: 2025-3-28 17:33
https://doi.org/10.1007/978-1-59745-335-6BAC; Carcinom; Chromosom; DNA; Microarray; Translation; gene expression; genes; hybridization; signal transdu作者: 善變 時(shí)間: 2025-3-28 21:35 作者: Bridle 時(shí)間: 2025-3-29 01:28
Cloning Differentially Expressed Genes Using Rapid Subtraction Hybridization (RaSH),ance or sensitivity to HIV-1 in human T cells and gene expression changes following infection of normal human fetal astrocytes with HIV-1 or treatment with neutrotoxic agents. Based on its simplicity of performance and high frequency of genuine differential gene identification, the rapid subtraction作者: 魔鬼在游行 時(shí)間: 2025-3-29 04:33 作者: 嘴唇可修剪 時(shí)間: 2025-3-29 09:57
Gene Profiling Uncovers Retinoid Target Genes,ell differentiation, induce growth suppression, trigger apoptosis or affect other growth regulatory pathways. Pathways involved in the regulation of protein stability will be highlighted as these play a critical role in mediating pharmacological effects of the retinoids in cancer therapy or chemopre作者: 使隔離 時(shí)間: 2025-3-29 15:06 作者: ANNUL 時(shí)間: 2025-3-29 17:48 作者: 簡(jiǎn)潔 時(shí)間: 2025-3-29 22:55
Molecular Cytogenetic Applications in Analysis of the Cancer Genome,mosomes in one experiment permitting identification of precise chromosomal changes on metaphases derived from tumor cells. The uses of fluorescence . hybridization are diverse, including mapping of alteration in single copy genes, chromosomal regions, or entire chromosomes. The opportunities to dete作者: Pelvic-Floor 時(shí)間: 2025-3-30 00:10
Ribonomic and Short Hairpin RNA Gene Silencing Methods to Explore Functional Gene Programs Associatry of shRNAs that target p38 induced mRNAs expression libraries are utilized to “knockdown” the genes identified in the first section. Finally, this library of gene candidates is evaluated in vivo to address their functional role in the induction or maintenance of dormancy.作者: 能夠支付 時(shí)間: 2025-3-30 07:05 作者: Ceramic 時(shí)間: 2025-3-30 09:13
PKR in Innate Immunity, Cancer, and Viral Oncolysis,nases, in addition to PKR, following exposure to such cellular stresses as amino acid deprivation and the presence of misfolded proteins in the endoplasmic reticulum. Indeed, it has been demonstrated that disruption of the eIF2α checkpoint can lead to the transformation of immortalized rodent and hu作者: MAIZE 時(shí)間: 2025-3-30 12:29
Analysis of Transformation and Tumorigenicity Using Mouse Embryonic Fibroblast Cells,ovided a powerful tool to analyze the genetic regulation of these cellular processes. In this chapter, methods for analysis of cellular immortalization using the 3T3 protocol, as well as transformation of MEF cells using oncogenic retroviruses are provided. This is followed by protocols for analysis作者: 表示向前 時(shí)間: 2025-3-30 19:19 作者: Demonstrate 時(shí)間: 2025-3-30 23:45 作者: 下邊深陷 時(shí)間: 2025-3-31 01:57
Arto K?li?,Mari Honkanen,Jukka Lahdensivuell differentiation, induce growth suppression, trigger apoptosis or affect other growth regulatory pathways. Pathways involved in the regulation of protein stability will be highlighted as these play a critical role in mediating pharmacological effects of the retinoids in cancer therapy or chemopre作者: BRACE 時(shí)間: 2025-3-31 05:39
Diana Kopeva,Nikolay Sterev,Dimitar Blagoevons, in practice it has been successfully applied to clone both known and unknown genes with varying regions of GC-rich content. C-ORF does not use additional enzymes other than reverse transcriptase and . polymerase making it a cost-effective and relatively simple method that should be of general u作者: Heretical 時(shí)間: 2025-3-31 10:34 作者: ADJ 時(shí)間: 2025-3-31 14:23
Renu Jatana,Mehjabeen Barodawalamosomes in one experiment permitting identification of precise chromosomal changes on metaphases derived from tumor cells. The uses of fluorescence . hybridization are diverse, including mapping of alteration in single copy genes, chromosomal regions, or entire chromosomes. The opportunities to dete作者: Urea508 時(shí)間: 2025-3-31 20:41 作者: agenda 時(shí)間: 2025-3-31 23:12
F. Pervin Bilir,Seyhan Bilir Gülercomplicated and effective means of developing MAbs, which can also be used to identify genes, associated with important cellular processes involved in normal physiology, such as growth, aging, differentiation, and development. In addition, this strategy is amenable to produce MAbs and identify genes作者: 心胸狹窄 時(shí)間: 2025-4-1 05:00
Merve ?ztürk,Muhammet Bezirci,Yunus Cerannases, in addition to PKR, following exposure to such cellular stresses as amino acid deprivation and the presence of misfolded proteins in the endoplasmic reticulum. Indeed, it has been demonstrated that disruption of the eIF2α checkpoint can lead to the transformation of immortalized rodent and hu作者: 不滿分子 時(shí)間: 2025-4-1 09:21
Merve ?ztürk,Muhammet Bezirci,Yunus Ceranovided a powerful tool to analyze the genetic regulation of these cellular processes. In this chapter, methods for analysis of cellular immortalization using the 3T3 protocol, as well as transformation of MEF cells using oncogenic retroviruses are provided. This is followed by protocols for analysis作者: 偶然 時(shí)間: 2025-4-1 10:53
1064-3745 s, and biochemists involved in studying genes associated with and regulating important physiological processes. This volume will serve as a valuable laboratory resource for designing experiments to identify and978-1-61737-605-4978-1-59745-335-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 開始從未 時(shí)間: 2025-4-1 14:21 作者: Directed 時(shí)間: 2025-4-1 21:47 作者: AGATE 時(shí)間: 2025-4-1 23:55
The Application of Differential Display as a Gene Profiling Tool,aboratories, several examples of successful applications of differential display under a number of scenarios are reviewed. Differential display is one of several expression profiling technologies available and is compared with some of them. The future of differential display remains bright and is as applicable today as it was in 1992.作者: 前兆 時(shí)間: 2025-4-2 06:42 作者: 整理 時(shí)間: 2025-4-2 10:04
