標(biāo)題: Titlebook: Cancer Cell Culture; Methods and Protocol Dania Movia,Adriele Prina-Mello Book 2023 The Editor(s) (if applicable) and The Author(s), under [打印本頁(yè)] 作者: False-Negative 時(shí)間: 2025-3-21 19:51
書目名稱Cancer Cell Culture影響因子(影響力)
書目名稱Cancer Cell Culture影響因子(影響力)學(xué)科排名
書目名稱Cancer Cell Culture網(wǎng)絡(luò)公開度
書目名稱Cancer Cell Culture網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cancer Cell Culture被引頻次
書目名稱Cancer Cell Culture被引頻次學(xué)科排名
書目名稱Cancer Cell Culture年度引用
書目名稱Cancer Cell Culture年度引用學(xué)科排名
書目名稱Cancer Cell Culture讀者反饋
書目名稱Cancer Cell Culture讀者反饋學(xué)科排名
作者: Distribution 時(shí)間: 2025-3-21 22:35
Generation and Characterization of an Isogenic Cell Line Model of Radioresistant Esophageal Adenocar by chronic irradiation of esophageal adenocarcinoma cells with clinically relevant doses of X-ray radiation. We also characterize cell cycle, apoptosis, reactive oxygen species (ROS) production, DNA damage and repair in this model to investigate the?underlying molecular mechanisms of radioresistance in esophageal adenocarcinoma.作者: thrombus 時(shí)間: 2025-3-22 02:13 作者: 除草劑 時(shí)間: 2025-3-22 05:08
Developing an In Vitro Isogenic Model of Chemotherapy-Resistant Lung Cancerenge for oncologists. In order to study and elucidate the cellular and molecular mechanisms implicated in the development of drug resistance in cancer, the use of isogenic models provides a valuable in vitro tool for investigating novel biomarkers and the identification of potential targetable pathways involved in drug-resistant cancers.作者: Custodian 時(shí)間: 2025-3-22 12:25
Culturing Human Lung Adenocarcinoma Cells in a Serum-Free Environmentading to possible reproducibility issues in experiments and readouts. This chapter describes how to transition A549 cells to FBS-free medium and gives some insights on the further characterizations and functionality assays that would be necessary to perform for the validation of the cultured cells.作者: fallible 時(shí)間: 2025-3-22 13:05
A Method for Culturing 3D Tumoroids of Lung Adenocarcinoma Cells at the Air–Liquid Interfaceck polarity. Our method allows to overcome this limitation by enabling to grow tumoroids of lung adenocarcinoma cells and healthy lung fibroblasts at the Air–Liquid Interface (ALI). This ensures straightforward access to both the apical and basal surface of the cancer cell culture, with several advantages in drug screening applications.作者: fallible 時(shí)間: 2025-3-22 20:57
Isolation and Cryopreservation of Mononuclear Cells from Peripheral Blood and Bone Marrow of Blood Cfugation. The cells obtained using the protocol described can be further purified for a variety of cellular, immunological, molecular, and functional assays. In addition, these cells can be cryopreserved and bio-banked for future research studies.作者: 富饒 時(shí)間: 2025-3-22 23:28 作者: 親愛 時(shí)間: 2025-3-23 01:47 作者: cushion 時(shí)間: 2025-3-23 08:38
Joan P. Schwartz,Ann M. Marini,R. W. Rodieck, we describe a methodology to generate 3D liver spheres from pluripotent stem cells. Liver spheres are?composed?of?three key liver cell types (hepatic progenitor cells, endothelial cells, and hepatic stellate cells)?and?have been used to study human cancer?cell metastasis.作者: 薄荷醇 時(shí)間: 2025-3-23 11:40 作者: Adrenal-Glands 時(shí)間: 2025-3-23 17:11 作者: entreat 時(shí)間: 2025-3-23 19:30 作者: Lipoprotein(A) 時(shí)間: 2025-3-23 22:23
Serum-Free Production of Human Stem Cell-Derived Liver Spheres for Cancer Metastasis Research, we describe a methodology to generate 3D liver spheres from pluripotent stem cells. Liver spheres are?composed?of?three key liver cell types (hepatic progenitor cells, endothelial cells, and hepatic stellate cells)?and?have been used to study human cancer?cell metastasis.作者: BARB 時(shí)間: 2025-3-24 05:11 作者: ENNUI 時(shí)間: 2025-3-24 07:27 作者: 排出 時(shí)間: 2025-3-24 12:41
Book 2023ensional culturing techniques. The chapters in this book are divided into two parts. Part One discusses two-dimensional cancer cell culture, cell models at the Air-Liquid Interface, and the latest advancements in three-dimensional complex spheroid models and dedicated disease animal models. Part Two作者: exigent 時(shí)間: 2025-3-24 18:30
1064-3745 ation advice from the experts.This volume explores the latest collection of cell models that are used in preclinical cancer research, and covers both two-dimensional and three-dimensional culturing techniques. The chapters in this book are divided into two parts. Part One discusses two-dimensional c作者: 無(wú)脊椎 時(shí)間: 2025-3-24 21:17 作者: apiary 時(shí)間: 2025-3-25 01:27 作者: GRIN 時(shí)間: 2025-3-25 06:49
A Short History of Neuroscience,lfare..The aim of this chapter is not to list and address all animal models used in cancer research. Instead, the authors would like to guide experimenters in the strategies to adopt in both planning and performing in vivo experimental procedures, including the choice of cancer animal models.作者: 蚊帳 時(shí)間: 2025-3-25 08:38
A Short History of Neuroscience,enge for oncologists. In order to study and elucidate the cellular and molecular mechanisms implicated in the development of drug resistance in cancer, the use of isogenic models provides a valuable in vitro tool for investigating novel biomarkers and the identification of potential targetable pathways involved in drug-resistant cancers.作者: 清醒 時(shí)間: 2025-3-25 14:46
A Short History of Neuroscience,ading to possible reproducibility issues in experiments and readouts. This chapter describes how to transition A549 cells to FBS-free medium and gives some insights on the further characterizations and functionality assays that would be necessary to perform for the validation of the cultured cells.作者: 織布機(jī) 時(shí)間: 2025-3-25 19:45
The Brain Positioning Software,ck polarity. Our method allows to overcome this limitation by enabling to grow tumoroids of lung adenocarcinoma cells and healthy lung fibroblasts at the Air–Liquid Interface (ALI). This ensures straightforward access to both the apical and basal surface of the cancer cell culture, with several advantages in drug screening applications.作者: 使殘廢 時(shí)間: 2025-3-25 20:43 作者: OVER 時(shí)間: 2025-3-26 03:36
https://doi.org/10.1007/978-3-031-62336-3without adding extracellular matrix components, and the elimination of the critique handling process needed for transferring spheroids. Although this protocol exemplifies the determination of percentage cell viability in PC-3 prostate tumor spheroids, it can be adapted and optimized for other prostate cell lines and other types of cancers.作者: 法律的瑕疵 時(shí)間: 2025-3-26 05:59
Cancer Cell Culture: The Basics and Two-Dimensional Culturesn this fast-paced industry. From basic monolayer cultures and functional assays to more recent and ever-advancing cell-based cancer interventions, 2D cell culture plays a crucial role in cancer diagnosis, prognosis, and treatment. Research and development in this field call for a great deal of optim作者: 新奇 時(shí)間: 2025-3-26 12:01 作者: 惡名聲 時(shí)間: 2025-3-26 13:18 作者: Noisome 時(shí)間: 2025-3-26 18:47 作者: 繁忙 時(shí)間: 2025-3-27 00:57
Generation of Radioresistant Prostate Cancer Cellsate the mechanisms of radioresistance in cancer cells. Owing to the complex nature of the biological effect of ionizing radiation, the generation and validation of these models requires the careful consideration of radiation exposure protocols and cellular endpoints. This chapter presents a protocol作者: neoplasm 時(shí)間: 2025-3-27 04:50
Generation and Characterization of an Isogenic Cell Line Model of Radioresistant Esophageal Adenocarlay treatment resistance. The molecular pathways leading to treatment resistance in cancer have been subject to research for many years. Isogenic cell lines with divergent radiosensitivities are an extremely useful tool to study the molecular mechanisms that underpin radioresistance in cancer resear作者: RADE 時(shí)間: 2025-3-27 06:05 作者: 最小 時(shí)間: 2025-3-27 10:31 作者: Ossification 時(shí)間: 2025-3-27 15:53
A Method for Culturing 3D Tumoroids of Lung Adenocarcinoma Cells at the Air–Liquid Interfacein the investigation of tumor growth and proliferation, invasion, and drug screening. However, 3D tumor spheroids and tumoroids cannot fully mimic the architecture of the human lung adenocarcinoma tissue and, in particular, the direct contact of the lung adenocarcinoma cells with the air, as they la作者: Climate 時(shí)間: 2025-3-27 19:43
Isolation and Cryopreservation of Mononuclear Cells from Peripheral Blood and Bone Marrow of Blood Cource of patient-specific cancer cells, as well as non-malignant cells, for research proposes. The simple and reproducible method presented here allows isolation of viable mononuclear cells, including malignant cells, from fresh peripheral blood or bone marrow aspirates using density gradient centri作者: 禁止 時(shí)間: 2025-3-27 23:44 作者: lambaste 時(shí)間: 2025-3-28 03:05 作者: bromide 時(shí)間: 2025-3-28 07:29 作者: Diluge 時(shí)間: 2025-3-28 10:24
A Method for the In Vitro Cytotoxicity Assessment of Anti-cancer Compounds and Materials Using High tial toxicity. This issue not only causes a high attrition rate for these compounds but also slows down the drug discovery process in general. To overcome this problem, robust, accurate, and reproducible methodologies for assessing anti-cancer compounds are essential. Multiparametric technique and h作者: llibretto 時(shí)間: 2025-3-28 17:57 作者: 考博 時(shí)間: 2025-3-28 21:11
Cell Viability Assay with 3D Prostate Tumor Spheroidsfor measuring the cell viability of 3D in vitro models. Here, we describe how to form 3D prostate tumor spheroids using the polyHEMA technique, apply drug treatments and WST-8 assay to these spheroids, and calculate their cell viability. The advantages of our protocol are the formation of spheroids 作者: falsehood 時(shí)間: 2025-3-29 02:35 作者: craven 時(shí)間: 2025-3-29 05:43
Ephaptic Interactions Among Axons,res of the human in vivo epithelial barriers (e.g., lung, intestine, and skin), ALI cell cultures enable proper structural architectures and differentiated functions of normal and diseased tissue barriers. Thereby, ALI models realistically resemble tissue conditions and provide in vivo-like response作者: 抵消 時(shí)間: 2025-3-29 07:22 作者: 撤退 時(shí)間: 2025-3-29 12:26
A Short History of Neuroscience,. Animals are used at multiple levels, from understanding molecular traits and pathways, to mimicking clinical aspects of tumor progression, to drug testing. In vivo approaches are not trivial and involve cross-disciplinary knowledge: animal biology and physiology, genetics, pathology, and animal we作者: IDEAS 時(shí)間: 2025-3-29 18:06 作者: Munificent 時(shí)間: 2025-3-29 19:53 作者: incontinence 時(shí)間: 2025-3-30 03:16
A Short History of Neuroscience,tin is still one of the most commonly used treatments for advanced NSCLC patients in the absence of oncogenic driver mutations or immune checkpoints. Unfortunately, as in the case of many solid tumors, acquired drug resistance is a common phenomenon in NSCLC and presents a significant clinical chall作者: curettage 時(shí)間: 2025-3-30 07:30
A Short History of Neuroscience, are frequently cultured in Ham’s F12K (Kaighn’s) or Dulbecco’s Modified Eagle’s Medium (DMEM), supplemented with glutamine and 10% fetal bovine serum (FBS). However, the use of FBS presents significant scientific concerns, such as the presence of undefined components and batch-to-batch variation le作者: Airtight 時(shí)間: 2025-3-30 09:38
The Brain Positioning Software,in the investigation of tumor growth and proliferation, invasion, and drug screening. However, 3D tumor spheroids and tumoroids cannot fully mimic the architecture of the human lung adenocarcinoma tissue and, in particular, the direct contact of the lung adenocarcinoma cells with the air, as they la作者: 健壯 時(shí)間: 2025-3-30 15:05
https://doi.org/10.1007/978-1-4899-6754-1ource of patient-specific cancer cells, as well as non-malignant cells, for research proposes. The simple and reproducible method presented here allows isolation of viable mononuclear cells, including malignant cells, from fresh peripheral blood or bone marrow aspirates using density gradient centri作者: 漫不經(jīng)心 時(shí)間: 2025-3-30 19:14
Joan P. Schwartz,Ann M. Marini,R. W. Rodieckd models provide?an alternative to cell lines, which can display limited relevance to primary cells and tissue. Historically, two-dimensional (2D) cultures have been used to model liver biology as they are easy to scale and deploy. However, 2D liver models lack functional diversity and phenotypic st作者: 編輯才信任 時(shí)間: 2025-3-31 00:11 作者: Ruptured-Disk 時(shí)間: 2025-3-31 04:07 作者: HIKE 時(shí)間: 2025-3-31 05:45 作者: Infinitesimal 時(shí)間: 2025-3-31 11:34
Neurocultural Health and Wellbeings that are stimulated by alternating magnetic fields to induce temperature elevations in tumor tissue, resulting in cell death. Magnetic hyperthermia is clinically approved for treating glioblastoma in Europe and is undergoing clinical evaluation for prostate cancer in the United States. Numerous st作者: 貪婪地吃 時(shí)間: 2025-3-31 16:04
