標(biāo)題: Titlebook: Calpain; Methods and Protocol Jeannette S. Messer Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 proteases.en [打印本頁] 作者: 會(huì)議記錄 時(shí)間: 2025-3-21 19:35
書目名稱Calpain影響因子(影響力)
書目名稱Calpain影響因子(影響力)學(xué)科排名
書目名稱Calpain網(wǎng)絡(luò)公開度
書目名稱Calpain網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Calpain被引頻次
書目名稱Calpain被引頻次學(xué)科排名
書目名稱Calpain年度引用
書目名稱Calpain年度引用學(xué)科排名
書目名稱Calpain讀者反饋
書目名稱Calpain讀者反饋學(xué)科排名
作者: Gustatory 時(shí)間: 2025-3-21 20:41
Bacterial Expression and Purification of Calpainscation of recombinant rat calpain-2 in bacteria was essential for solving the first crystal structures of the calpains in both calcium-free and calcium-bound forms. Here we describe the production and purification of recombinant rat calpain-2 from . using anion-exchange, affinity, and size-exclusion作者: 重疊 時(shí)間: 2025-3-22 01:43 作者: 薄膜 時(shí)間: 2025-3-22 06:53 作者: 冷峻 時(shí)間: 2025-3-22 11:36
In Vivo Monitoring of Calpain Activity by Forster Resonance Energy Transferlated in pathological conditions. Some are ubiquitously expressed (CAPN1, CAPN2, CAPN4, CAPN5, CAPN7, and CAPN10), but others are thought to be localized in specific tissues. The monitoring of in vivo calpain activity is required for physiological, pathological, and therapeutic evaluations. This pas作者: Debark 時(shí)間: 2025-3-22 15:56 作者: Debark 時(shí)間: 2025-3-22 18:29
Immunohistochemical Localization of Calpains in the Amphibian ssues and provide a large amount of information in a relatively short time and at a low cost. Here we describe methods for indirect immunohistochemistry and immunofluorescence on sections of tadpoles and tissues of adult amphibians belonging to the species . The objective is to localize calpains wit作者: fatty-streak 時(shí)間: 2025-3-22 23:58
Expression and Activity of Calpain A in s cytosol-enriched fractions for measuring calpain activity with the fluorogenic substrate N-LY-AMC. With this method one can measure calpain A activity in wild-type flies and in several mutant fly backgrounds, revealing a strong correlation between in situ membrane distribution and in vitro determi作者: Intend 時(shí)間: 2025-3-23 01:48 作者: Flatter 時(shí)間: 2025-3-23 06:41 作者: foreign 時(shí)間: 2025-3-23 13:44 作者: 哄騙 時(shí)間: 2025-3-23 15:20
Identification of Calpain-Activated Protein Functionsbstrates. The cleavage of some substrates can produce active fragments that perform functions that are different from those performed by the full-length proteins. Therefore, cleavage by calpains can operate as a posttranslational modification and increase the functional diversity of target proteins.作者: finale 時(shí)間: 2025-3-23 19:36 作者: 音樂學(xué)者 時(shí)間: 2025-3-23 23:21
Isolation of Endogenous Calpastatinhibitory activity against calpain in the presence of Ca. to specifically identify the protein. Thus, the purification steps necessary to obtain the inhibitor protein were originally designed to obtain calpain from the same tissue. For this reason, in addition to calpastatin purification, we also inc作者: 易于 時(shí)間: 2025-3-24 04:54
Immunoblotting for Calpastatin Expression calpastatin isoforms can vary by tissue, can be modified by partial digestion, and can undergo posttranslational modifications. Here we describe a method for more precise evaluation of calpastatin expression by combining immunoblot analysis with an assay for the inhibitory activity of a single calp作者: 手銬 時(shí)間: 2025-3-24 10:23 作者: 束以馬具 時(shí)間: 2025-3-24 13:12
Single-Step Purification of Calpain-1, Calpain-2, and Calpastatin Using Anion-Exchange Chromatographs that effectively bind with the DEAE Sephacel and separate well using a stepwise, increasing gradient of NaCl to elute the proteins. Calpastatin binds most weakly with the column matrix, so it elutes first, followed by calpain-1 and, finally, calpain-2.作者: Adenoma 時(shí)間: 2025-3-24 15:38
Casein Zymography for Analysis of Calpain-1 and Calpain-2 Activityvoid of dye when stained with Coomassie Brilliant Blue G-250. We describe here how calpains can be extracted from muscle tissue and assayed for activity using this technique. This technique is also generally applicable to lysates from other types of cells or tissues.作者: 抱狗不敢前 時(shí)間: 2025-3-24 20:14
Mara B. Antonoff,Ashok K. Salujas that effectively bind with the DEAE Sephacel and separate well using a stepwise, increasing gradient of NaCl to elute the proteins. Calpastatin binds most weakly with the column matrix, so it elutes first, followed by calpain-1 and, finally, calpain-2.作者: Gerontology 時(shí)間: 2025-3-25 01:41 作者: 機(jī)警 時(shí)間: 2025-3-25 05:48
1064-3745 ation advice from the experts.This volume brings together a plethora of protocols and experimental methods used by scientists to study calpains, their inhibitors, and their substrates. It also explores bioinformatic approaches to calpain substrate identification. The chapters in this book are divide作者: 我要沮喪 時(shí)間: 2025-3-25 08:35
,Mechanism of Action of α-Methyl-Dopa,e describe two methods to determine temporal and spatial expression of Calpain 2 during . development, namely, RT-PCR and whole-mount in situ hybridization (WISH). In addition, indirect immunofluorescence (IF) is described to determine translocation to the plasma membrane, which correlates with activity levels of Calpain 2.作者: flimsy 時(shí)間: 2025-3-25 13:10
Cystathioninuria and Vanil-lactic-acid-uria,ry and immunofluorescence on sections of tadpoles and tissues of adult amphibians belonging to the species . The objective is to localize calpains within tissues in order to understand their involvement in cellular processes.作者: 能夠支付 時(shí)間: 2025-3-25 18:02
https://doi.org/10.1007/978-3-319-24639-0ty in wild-type flies and in several mutant fly backgrounds, revealing a strong correlation between in situ membrane distribution and in vitro determined activity measurements. Here we describe the steps for tissue preparation and calpain activity measurement in the . embryo.作者: Flu表流動(dòng) 時(shí)間: 2025-3-25 22:55
Imaging of Vertebral Brucellosisam of the ./. reporter gene, you visualize when the promoter is activating expression by monitoring enzymatic activity of GUS, by detecting β-glucuronidase cleavage products. Here we describe a protocol for monitoring the . promoter activity in different tissues in . by GUS staining.作者: Asparagus 時(shí)間: 2025-3-26 00:17 作者: Heterodoxy 時(shí)間: 2025-3-26 05:07
Metabolic Correlates of Focal Ischemia,thod for more precise evaluation of calpastatin expression by combining immunoblot analysis with an assay for the inhibitory activity of a single calpastatin species isolated by SDS-PAGE and protein elution from the gel.作者: 清洗 時(shí)間: 2025-3-26 09:54 作者: 我還要背著他 時(shí)間: 2025-3-26 14:30
Immunohistochemical Localization of Calpains in the Amphibian ry and immunofluorescence on sections of tadpoles and tissues of adult amphibians belonging to the species . The objective is to localize calpains within tissues in order to understand their involvement in cellular processes.作者: 藝術(shù) 時(shí)間: 2025-3-26 17:37
Expression and Activity of Calpain A in ty in wild-type flies and in several mutant fly backgrounds, revealing a strong correlation between in situ membrane distribution and in vitro determined activity measurements. Here we describe the steps for tissue preparation and calpain activity measurement in the . embryo.作者: NEG 時(shí)間: 2025-3-26 21:02 作者: Palpable 時(shí)間: 2025-3-27 01:48 作者: Cardiac 時(shí)間: 2025-3-27 07:56 作者: 滴注 時(shí)間: 2025-3-27 11:31 作者: 混沌 時(shí)間: 2025-3-27 14:03 作者: Feckless 時(shí)間: 2025-3-27 20:13 作者: AORTA 時(shí)間: 2025-3-28 01:20
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/220938.jpg作者: Sigmoidoscopy 時(shí)間: 2025-3-28 02:10 作者: Dawdle 時(shí)間: 2025-3-28 09:04
Springer Science+Business Media, LLC, part of Springer Nature 2019作者: output 時(shí)間: 2025-3-28 13:52
Calpain978-1-4939-8988-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 天真 時(shí)間: 2025-3-28 14:43 作者: 土坯 時(shí)間: 2025-3-28 21:26 作者: Pelago 時(shí)間: 2025-3-29 02:19 作者: 飛鏢 時(shí)間: 2025-3-29 03:55
Techniques for Imaging with mIBG,sile bond. As with most proteases, they mainly cut unstructured or extended regions of their target proteins. The tendency for concentrated calpain to rapidly autoproteolyze when activated by calcium complicates the kinetic assessment of calpain activity. As calpain autoproteolyzes, the amount of fu作者: 幼稚 時(shí)間: 2025-3-29 09:09
Case Studies with Abnormal Uptake of mIBG,lated in pathological conditions. Some are ubiquitously expressed (CAPN1, CAPN2, CAPN4, CAPN5, CAPN7, and CAPN10), but others are thought to be localized in specific tissues. The monitoring of in vivo calpain activity is required for physiological, pathological, and therapeutic evaluations. This pas作者: Encephalitis 時(shí)間: 2025-3-29 11:29
,Mechanism of Action of α-Methyl-Dopa,pite the fact that Calpains have been identified in the . genome, their expression patterns and role have not been characterized. Therefore, herein, we describe two methods to determine temporal and spatial expression of Calpain 2 during . development, namely, RT-PCR and whole-mount in situ hybridiz作者: Fsh238 時(shí)間: 2025-3-29 19:18 作者: 施舍 時(shí)間: 2025-3-29 22:49
https://doi.org/10.1007/978-3-319-24639-0s cytosol-enriched fractions for measuring calpain activity with the fluorogenic substrate N-LY-AMC. With this method one can measure calpain A activity in wild-type flies and in several mutant fly backgrounds, revealing a strong correlation between in situ membrane distribution and in vitro determi作者: 責(zé)問 時(shí)間: 2025-3-30 01:34 作者: Obscure 時(shí)間: 2025-3-30 05:36 作者: CURB 時(shí)間: 2025-3-30 11:55 作者: GIST 時(shí)間: 2025-3-30 13:44
Excitatory Amino Acid Receptors in Brain,bstrates. The cleavage of some substrates can produce active fragments that perform functions that are different from those performed by the full-length proteins. Therefore, cleavage by calpains can operate as a posttranslational modification and increase the functional diversity of target proteins.作者: Climate 時(shí)間: 2025-3-30 17:36 作者: crease 時(shí)間: 2025-3-31 00:18
Takashi Watanabe,Takao Asano,Takao Shimizuhibitory activity against calpain in the presence of Ca. to specifically identify the protein. Thus, the purification steps necessary to obtain the inhibitor protein were originally designed to obtain calpain from the same tissue. For this reason, in addition to calpastatin purification, we also inc作者: 慟哭 時(shí)間: 2025-3-31 02:34
Metabolic Correlates of Focal Ischemia, calpastatin isoforms can vary by tissue, can be modified by partial digestion, and can undergo posttranslational modifications. Here we describe a method for more precise evaluation of calpastatin expression by combining immunoblot analysis with an assay for the inhibitory activity of a single calp作者: Offstage 時(shí)間: 2025-3-31 07:31 作者: 帽子 時(shí)間: 2025-3-31 11:17 作者: 補(bǔ)助 時(shí)間: 2025-3-31 13:49